Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens

BACKGROUND: Food IgE-mediated allergy to members of the Brassicaceae family has been increasingly reported. OBJECTIVE: To characterize cabbage-Brassica oleracea var capitata-allergy and its major allergens. METHODS: A prospective study was performed, recruiting 17 patients allergic to cabbage, and control subjects. Skin prick tests and double-blind placebo-controlled food challenges were performed. A major allergen was isolated from cabbage by RP-HPLC and characterized by N-terminal amino acid sequencing and matrix-assisted laser desorption/ionization mass spectrometry analysis. Specific IgE determinations, IgE immunoblots, and CAP-inhibition assays were also performed. RESULTS: Skin prick test and specific IgE were positive to cabbage in all patients. Five of them referred anaphylactic reactions when eating cabbage, and in another 5 patients, cabbage allergy was further confirmed by double-blind placebo-controlled food challenge. Most of them showed associated sensitizations to mugwort pollen, mustard, and peach. A 9-kd cabbage IgE-binding protein, Bra o 3, was identified as a lipid transfer protein (LTP) with 50% of identity to peach LTP Pru p 3. Skin prick test with Bra o 3 showed positive results in 12 of 14 cases (86%). On CAP inhibition assays, Bra o 3 managed to inhibit significantly the IgE binding to cabbage, mugwort pollen, and peach. Both Bra o 3 and Pru p 3 were recognized by IgE from the patients' sera. CONCLUSION: Bra o 3, a cabbage LTP, is a major allergen in this food, cross-reacting with mugwort pollen and with other plant foods, such as peach. CLINICAL IMPLICATIONS: Cabbage IgE-mediated allergy is a potentially severe condition that can present with other plant food and pollen allergies.     

一种基于笔形束核的快速剂量计算方法

针对传统笔形束核剂量算法计算过程复杂、速度较慢的问题,提出一种基于笔形束核的快速剂量计算方法。该方法在球壳坐标系下,利用射束与不同球壳层碰撞后轴线与体素相交情况的相似性,通过对每条射束只计算初始球壳层碰撞点处轴线与体素的相交情况,并将初始球壳层深度与其它球壳层深度的比值作为校正因子得到其他层碰撞点处轴线与体素的相交情况。避免射线追踪法的重复使用,在不影响算法精度的情况下节省大量时间。实验结果表明:在不同射野大小［（3×3）、（5×5）、（10×10） cm2］的水模体中计算深度剂量分布,两种算法的计算精度基本一致,改进算法的计算速度提升约2.7倍。同时当射野面积为（10×10） cm2时,在肺阻块模体和骨阻块模体不同深度的剖面剂量计算中,改进算法与传统算法的计算精度也基本一致,但计算速度提升约2.6倍。     

正交法优选盐制小茴香工艺

[目的]优选小茴香的最佳炮制工艺。[方法]以反式茴香醚和水溶性浸出物的含量为评价指标,选择加盐量、炒制度及炒制间为考察因素,采用正交设计L9（3^4）,优选小茴香的最佳炮制工艺。[结果]小茴香的最佳炮制工艺为：小茴香每20g加15mL盐水（含0．4g盐）,闷润1．5h,在110～120℃下炒4min。[结论]盐制小茴香的最佳炮制工艺合理可行。     

桃仁脂肪酸GC-MS指纹图谱研究

目的:对桃仁脂肪酸进行气相色谱指纹图谱研究,为桃仁的质量评价提供依据.方法:利用毛细管气相色谱对桃仁脂肪酸进行分析,并利用GC-MS技术确认指纹图谱中的特征指纹信息,进行系统聚类和相似度分析.结果:对34批不同产地、品种的桃仁进行了测定,获得了较为理想的包含特征信息的桃仁GC指纹图谱.结论:本方法重复性好,所建立的指纹图谱为桃仁药材的质量控制提供了有效手段.     

基于仿生技术对薏苡仁麸炒过程中色泽气味变化研究

目的 对薏苡仁麸炒过程中的色泽与气味进行客观量化并对色泽与气味的变化特征进行研究。方法 采用CM-5型分光测色计（电子眼）及Heracles NEO超快速气相电子鼻技术，并通过判别分析、主成分分析（principal component analysis,PCA）、判别因子分析（discriminant factor analysis,DFA）、风味热图等方法对获取数据进行处理分析。结果 随着炮制程度的加深，薏苡仁外观色泽整体上呈现变暗（L*值不断降低）、变红（a*值不断增加）、变黄（b*值不断增加）的变化趋势，基于粉末色度值L*、a*、b*所构建的判别函数实现了生薏苡仁及不同炮制程度麸炒薏苡仁饮片的区分。薏苡仁固有及在麸炒过程中共检测出34种挥发性气味物质，随着炮制程度的加深，挥发性气味物质的丰富度不断提高。热图分析结果表明1-丁烯、2-丁烯、丙烯醛、乙硫醇、2-丁醇、乙偶姻、1,4-二氧六环、丙二醇、1-甲基-1H-吡咯、甲基环己烯、丁酸乙酯、丁基二甲硫醚、糠醇、辛内酯14种成分在薏苡仁麸炒过程中变化最为明显。PCA与DFA均可实现生薏苡仁及不同炮制程度麸炒薏苡仁饮片的区分。结论 ...     

丹参种子航天搭载的诱变效应研究

目的探讨丹参种子航天搭载的诱变效应。方法利用我国第二十颗返地式卫星搭载丹参种子,落地后田间种植观测。结果航天搭载可提高种子的出苗率,促进了幼苗的生长发育,植株的开花期提前1周左右。降低了植株的单株结实率,增加了单株籽粒重,显著提高了种子的千粒重。显著增加了地上的分支数和主果穗长度,提高了根鲜重。结论丹参种子航天搭载诱变的生物学效应,为丹参种质的改良和品种选育提供新的途径。     

基于《素问》运气七篇概念体系的运气理论范畴研究

范畴（理论范围与基本概念）是《内经》成功建构中医理论体系的关键。作为《内经》重要内容之一的运气理论，长期存在广义与狭义范畴理解的认识分歧。本研究以对《素问》运气七篇概念体系的梳理研究为基础，进行运气理论范畴讨论，认为《素问》运气七篇所述，虽以五运、六气、五六相合为标志和重点，但仍是对道即自然规律（包括生命与疾病规律）的阐述，用以探讨自然对生命影响的规律。因此，运气理论的范围涵盖广泛，涉及各种自然变化规律及其对生命（包括人与其他生命体）的影响，其知识领域的基本概念为运气七篇的核心概念，是对自然变化规律的普遍本质的高度概括和集中反映，包括道、五运、六气、五六相合以及形、气、象、数、正、化等。     

Particle Size Measurement Using Dynamic Light Scattering at Ultra-Low Concentration Accounting for Particle Number Fluctuations

Dynamic light scattering (DLS) is a popular method of particle size measurement, but at ultra-low particle concentrations, the occurrence of number concentration fluctuations limits the use of the technique. Number fluctuations add a non-Gaussian term to the scattered light intensity autocorrelation function (ACF). This leads to an inaccurate particle size distribution (PSD) being recovered if the normal DLS analysis model is used. We propose two methods for inverting the DLS data and recovering the PSDs when number fluctuations are apparent. One is to directly establish the relationship between the non-Gaussian ACF and the PSD by the kernel function reconstruction (KFR) method while including the non-Gaussian term to recover the PSD. The other is to remove the effect of the non-Gaussian term in the ACF by the baseline reset (BR) method. By including the number fluctuation term, the ideal recovered PSD can be obtained from the simulated data, but this will not happen in the experimental measurement data. This is because the measured intensity ACF contains more noise than the simulated ACF at ultra-low concentration. In particular, the baseline noise at the tail of long delay time of ACF overwhelms the number fluctuation term, making it difficult to recover reliable PSD data. Resetting the baseline can effectively remove the digital fluctuation term in ACF, which is also a feasible method to improve PSD recovery under ultra-low concentration. However, increasing noise at ultra-low concentrations can lead to errors in determining an effective baseline. This greatly reduces the accuracy of inversion results. Results from simulated and measured ACF data show that, for both methods, noise on the ACF limits reliable PSD recovery.     

用能量沉积核函数方法计算60Co照射野的吸收剂量

目的 介绍了用能量沉积核函数方法计算＾６０Ｃｏ照射野吸收剂理的方法。方法 能量沉积核函数方法将吸收剂量的贡献分为３部分：原射线、单次散射和多次散射。它使用基本的剂量学数据,如射野中心轴百分深度剂量、离轴比和淮直系统散射输出因子等,这些数据在Ｆｙｃ５０Ｈ治疗机上用方形照射野测量得到,再用能量沉积核函数计算吸收剂量,并讨论了散射线对吸收剂量的影响。结果 从测量数据得到了原射线和散射线的能量沉积核函数,并利用能量沉积核函数计算＾６０Ｃｏ照射野的主要剂量学参数。计算值和测量值是一致的;不规则照射野的吸收剂量及其分布的计算结果也和测量结果符合得很好。结论 能量沉积核函数方法适用于较精确地计算＾６０Ｃｏ不规则照射野的吸收剂量。