埋线联合盐酸依匹斯汀治疗慢性荨麻疹的临床疗效

目的：观察盐酸依匹斯汀联合穴位埋线治疗慢性荨麻疹的临床疗效。方法：140例患者随机分两组,治疗组72例采用穴位埋线联合口服依匹斯汀,对照组68例单口服盐酸依匹斯汀,60d后进行疗效评定。结果：治疗组总有效率为88.89%,对照组总有效率为75%。观察期间未见严重不良反应。结论：穴位埋线联合口服药物治疗慢性荨麻疹,疗效显著,安全可靠,副作用小。     

微球固定化蜗牛酶转化人参皂苷Rb1制备人参稀有皂苷Compound K研究

目的制备微球固定化蜗牛酶,并优选出微球固定化蜗牛酶转化人参皂苷Rb1(Rb1)制备人参稀有皂苷Compound K(CK)的最佳制备工艺。方法采用交联-包埋法制备微球固定化蜗牛酶,以酶活力回收率为考察指标,通过正交试验优选出最佳制备工艺;考察了最适反应温度、最适反应p H值、热稳定性、p H稳定性和储存稳定性等酶学性质,并通过单因素考察转化温度、底物浓度、转化时间和固定化蜗牛酶使用次数对转化率的影响,优化制备工艺。结果固定化蜗牛酶的最佳制备工艺:海藻酸钠质量浓度2%,Ca Cl2质量浓度2%,Si O2与蜗牛酶质量比1∶1,在此条件下固定化蜗牛酶的酶活回收率为81.94%;固定化蜗牛酶与游离蜗牛酶在热稳定性与p H值稳定性方面显示出不同的性质,其中固定化蜗牛酶的最适反应温度为60℃,最适反应p H值为5.0。固定化蜗牛酶在15℃环境下保存30 d后,酶活回收率为55.17%。固定化蜗牛酶转化Rb1制备人参稀有皂苷CK的转化条件:转化温度为55℃,底物质量浓度为1.0 mg/m L,转化时间为36 h,转化次数为5次,平均转化率为36.79%。结论蜗牛酶的固定化增强了其稳定性和使用寿命,且人参稀有皂苷CK的转化率提高,工艺简单,适合工业化生产。     

壮医药线点灸配合针刺治疗失眠症疗效观察

目的 观察壮医药线点灸配合针刺治疗失眠症的临床疗效。方法 将95例失眠症患者随机分为治疗组50例和对照组45例。治疗组采用壮医药线点灸配合针刺治疗,对照组采用单纯针刺治疗。比较两组治疗前后睡眠质量指数（PSQI）评分和临床疗效。结果 治疗组治疗1、2个疗程后PSQI评分与同组治疗前比较,差异均具有统计学意义（P＜0.05,P＜0.01）。对照组治疗2个疗程后PSQI评分与同组治疗前比较,差异具有统计学意义（P＜0.05）。治疗组治疗2个疗程后PSQI评分与对照组比较,差异具有统计学意义（P＜0.05）。治疗组总有效率为90.0%,对照组为71.1%,两组比较差异具有统计学意义（P＜0.05）。结论 壮医药线点灸配合针刺是一种治疗失眠症的有效方法。     

微创埋线用于针药复合麻醉对肺切除手术麻醉效果的影响

目的 比较在针药复合麻醉肺切除手术中运用微创埋线与全麻的麻醉效果,为针麻肺切除手术探索新的方法提供临床依据。方法 将50例肺切除手术患者随机分为治疗组和对照组,每组25例。治疗组术前予以微创埋线,取穴华佗夹脊（T4、T6、T8）与背俞穴（肺俞、心俞、膈俞）、曲池、足三里。对照组采用全麻。记录两组术中11个时间点的生理指标,计量术中麻醉药物实际使用量等指标。结果 两组术中11个时间点心率、收缩压与舒张压比较,差异均无统计学意义（P＞0.05）。治疗组术中芬太尼追加量与对照组比较,差异具有统计学意义（P＜0.05）。两组术中丙泊酚及罗库溴铵追加量比较,差异均无统计学意义（P＞0.05）。结论 微创埋线相对于全麻方案,能减少手术中镇痛药物的使用剂量,微创埋线与药物在肺切除手术中具有良好的协同作用。     

白环俞穴埋线术治疗环状混合痔术后疼痛的临床观察

目的观察白环俞穴埋线术对治疗混合痔手术后疼痛的效果。方法将本院中符合手术标准的环状混合痔患者100例,随机分为治疗组50例,术中应用白环俞穴埋线术;对照组50例,应用传统的克泽普注射液局部封闭法。分别观察3 d。结果在术后疼痛程度比较上,两组差异有统计学意义（P〈0.05）;在患者对止痛效果满意度及减少并发症发生几率上,治疗组明显优于对照组（P〈0.05）,差异有统计学意义。结论白环俞穴埋线术较传统止痛方法能更有效的减少并缓解环状混合痔术后疼痛,降低术后并发症的发生几率。     

DNA and RNA Isolated from tissues processed by microwave-accelerated apparatus MFX-800-3 are suitable for subsequent PCR and Q-RT-PCR amplification

Over the past decade, methods of molecular biology have appeared in diagnostic pathology and are routinely applied on formalin-fixed, paraffin-embedded histological samples, processed via conventional embedding methods. Due to its reagent- and cost-effectiveness, embedding techniques that utilize microwave acceleration in one or more steps of histoprocessing are increasingly used by numerous laboratories. The demand arises that tissues processed this way should also be suitable for the requirements of molecular pathology. In this study, both conventionally embedded and MFX-800-3 machine-processed tissue samples from the same source were used for isolation of DNA and RNA and for performing PCR and real-time PCR. PCR amplification of the β-globin gene, as well as the real-time PCR amplification of the ABL mRNA was successful in all cases. Our conclusion is that samples processed by the vacuum assisted automatic microwave histoprocessor MFX-800-3 are perfectly applicable for DNA and RNA isolation and provide appropriate templates for further PCR and realtime PCR studies.     

Development of a new flat needle and a reduced surface coating thread for endoscopic suturing

BACKGROUND: Intracorporeal suturing during endoscopic surgery is a difficult procedure. The curved needle is the most difficult to position properly within the jaws of the needle driver. The low friction suture materials exhibit good knot run-down, but at the expense of increased tendency for slipping. It is therefore necessary to invent a new needle and a new thread to facilitate intracorporeal suturing. MATERIALS AND METHODS: We made a new flat needle (F-needle) to facilitate grasping and a new reduced silicone-coated thread to prevent the knots from loosening. The time required to grasp the F-needle and the round needle (R-needle) by 10 surgeons was measured. The angle between the needle and the needle driver was measured. The minimum number of intertwining knots required for the knot to hold was counted, and the minimum number of superimposed knots that prevented the knots from coming undone when the testing machine attempted to release them was counted. RESULTS: The F-needle was grasped in less time than the R-needle. The error in yaw angle with the F-needle was smaller than in the R-needle. The minimum number of knots needed to hold against the untying force with the reduced silicone-coated thread was less than with the conventional silicone-coated thread. CONCLUSIONS: The new flat needle was grasped more accurately and quickly than the round needles, and the thread with reduced surface coating was less likely to loosen. Therefore, the combination of the new flat needle and thread with reduced surface coating is useful for endoscopic suturing.     

穿刺小标本改良石蜡块制作方法及评价

目的: 改良现有琼脂预包埋石蜡包埋法,评价该包埋法对空芯针穿刺活检小标本组织完整性、组织学形态、蛋白质和DNA检测的影响。方法: 取10例口腔黏膜鳞癌空芯针穿刺活检标本,分别进行成型包埋模具的改良琼脂预包埋石蜡包埋法及常规石蜡包埋法,前者缩短脱水时间至3.5 h,后者为12 h。样本处理后,分别行H-E染色、组织学形态、免疫组织化学（IHC）和DNA荧光原位杂交（FISH）等检测,并对检测结果进行对比分析。采用GraphPad Prism 9软件包对数据进行组间t检验。结果: 改良琼脂预包埋法较琼脂预包埋法降低了操作难度,易于推广。与常规石蜡包埋法相比,组织脱水时间显著缩短（P<0.001）,对镜下组织学形态及后续IHC和FISH检测无影响。结论: 改良琼脂预包埋石蜡包埋法满足临床病理诊断对组织处理的要求,是值得推广的空芯针穿刺活检标本组织包埋法。     

Eyebrow lifting using multidirectional absorbable thread

Background

Thread lifting is a common procedure in minimally invasive esthetic techniques and can also be used to raise the drooping soft tissue of the forehead by vertically inserting threads into the forehead.

Aims

This study aimed to examine the effect of forehead thread lifting on enhancing upper eyelid opening.

Methods

Fifteen patients were included in this study, all of whom underwent eyebrow thread lifting with MINT LIFT® UP. Photographs of the patients were taken before, immediately after, and 1 and 12 weeks after surgery. Changes in the position of the eyebrows and eyelids were measured. The paired t-test was used to determine the statistical significance of differences.

Results

At 1 week after surgery, the eyebrows were at a lower level compared with before surgery, and no significant changes in eye-opening were observed. However, at 12 weeks after surgery, the eyebrows and upper eyelids were both significantly elevated when compared to the preoperative state.

Conclusions

Eyebrow lifting can be performed using multidirectional thread lifting.