中医食疗配合中药内服治疗虚寒型胃溃疡18例

目的:观察中医食疗配合中药内服治疗虚寒型胃溃疡的临床效果。方法:选取本院2012年5月—2013年6月治疗的虚寒型胃溃疡患者36例,按照随机数字表法将36例患者分为观察组和对照组,每组18例。对照组给予中药内服治疗,观察组在对照组治疗的基础上给予中医食疗辅助治疗。结果:观察组有效率为94.44%,对照组有效率为66.67%,观察组优于对照组(P0.05);两组不良反应比较,差异无统计学意义(P0.05)。结论:中医食疗配合中药内服治疗虚寒型胃溃疡疗效明显。     

Altered osteoclast development and function in osteopontin deficient mice

The role of osteopontin in bone resorption was elucidated by studies of mice with knock out of the osteopontin gene generated by a different approach compared to previous models. Thus, a targeting vector with the promoter region as well as exons 1, 2, and 3 of the osteopontin gene was replaced by a loxP‐flanked Neo‐TK cassette, and this cassette was eliminated through transient expression of Cre recombinase. The recombined ES cells were used to create mice lacking expression of the osteopontin gene. Tissues from these mice were subjected structural and molecular analyses including morphometry and proteomics. The bone of the null mice contained no osteopontin but showed no significant alterations with regard to other bone proteins. The bone volume was normal in young null animals but in the lower metaphysis, the volume and number of osteoclasts were increased. Notably, the volume and length of the osteoclast ruffled border was several folds lower, indicating a lower resorptive capacity. The null mice did not develop the bone loss characteristic for osteoporosis demonstrated in old wild‐type female animals. This quantitative study demonstrates a bone phenotype in the osteopontin null mice of all ages. The data provides further evidence for a role of osteopontin in osteoclast activity. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:721–728, 2008     

Interictal lack of habituation of mismatch negativity in migraine

The aim was to study mismatch negativity features and habituation during the interictal phase of migraine. In migraine patients, a strong negative correlation has been found between the initial amplitude of long latency auditory-evoked potentials and their amplitude increase during subsequent averaging. We studied 12 outpatients with a diagnosis of migraine without aura recorded in a headache-free interval and 10 gender- and age-matched healthy volunteers not suffering from any recurrent headache. The experiment consisted of two sequential blocks of 2000 stimulations, during which 1800 (90%) recordings for standard tones and 200 (10%) for target tones were selected for averaging. The latency of the N1 component was significantly increased in migraine patients in respect of controls in both the first and second repetitions; the MMN latency was increased in the second repetition. In the control group the MMN amplitude decreased on average by 3.2 +/- 1.4 microV in the second trial, whereas in migraine patients it showed a slight increase of 0.21 +/- 0.11 microV in the second repetition. The MMN latency relieved in the second trial was significantly correlated with the duration of illness in the migraine patients (Spearman correlation coefficient: 0.69; P < 0.05). The increases in N1 latency and MMN latency and amplitude, the latter correlated with duration of illness, seemed to be due to a reduced anticipatory effect of stimulus repetition in migraine patients. This suggests that such hypo-activity of automatic cortical processes, subtending the discrimination of acoustic stimuli, may be a basic abnormality in migraine, developing in the course of the disease.     

Collection of mobilized peripheral blood stem cells from a donor with severe iron deficient anemia

We report a 45-year-old woman with iron deficient anemia (IDA) who underwent a collection of allogeneic peripheral blood stem cells (PBSCs) induced by granulocyte-colony stimulating factor (G-CSF) after a rapid improvement of IDA by iron replacement. Her peripheral red blood cells (RBCs) after iron therapy were composed of two different-sized subpopulations; one consisted of microcytes, which were iron deficient RBCs, and another of normocytes, which were produced after iron replacement. On the first day of PBSC collection, the interface setting was maintained aiming at 2% hematocrit as usual; however, PBSCs could not be collected adequately. Sedimentation of iron deficient, lighter RBCs under centrifugation within a blood cell separator could be similar to that of mononuclear cells, and the lighter RBCs could contaminate the mononuclear cell layer, resulting in the collection of the lighter layers of mononuclear cells than desired. On the second day, we succeeded in obtaining enough PBSCs by collecting heavier layers than those collected on the first day by using a 4% hematocrit and monitoring white blood cell counts of the collection line serially. It should be noted that the lighter RBCs from a donor with a history of IDA could complicate collection of PBSCs.     

葡萄糖－6－磷酸脱氢酶缺乏红细胞脂质过氧化的研究

按硫代巴比妥酸法测定了葡萄糖－６－磷酸脱氢酶（Ｇ６ＰＤ）缺乏和正常红细胞的丙二醛（ＭＤＡ）含量，按改良Ｎｉｓｓｅｌｂａｕｍ法测定还原型辅酶ＩＩ（ＮＡＤＰＨ）的含量。Ｇ６ＰＤ缺乏红细胞的ＭＤＡ和ＮＡＤＰＨ分别为３８．７４ｎｍｏｌ／ｇＨｂ和１８０３．６μｍｏｌ／ｇＨｂ；正常红细胞中含量则分别为２５．６０ｎｍｏｌ／ｇＨｂ和２２４２．８ｔＬｍｏｌ／ｇＨｂ。在受到过氧化刺激后，Ｇ６ＰＤ缺乏红细胞的ＭＤＡ生成明显大于正常红细胞，ＮＡＤＰＨ明显减少，而正常红细胞的ＮＡＤＰＨ则增加。维生素Ｅ能保护Ｇ６ＰＤ缺乏红细胞，使其在过氧化刺激下ＭＤＡ生成不增加。研究提示，Ｇ６ＰＤ缺乏红细胞的脂质过氧化损伤比正常严重，其机制是ＮＡＤＰＨ生成不足。     

肝乐冲剂治疗乙型肝炎肝郁脾虚型60例临床观察

[目的]探讨肝乐冲剂治疗乙型肝炎肝郁脾虚型的疗效。[方法]采用随机对照方法,将60例病人分为治疗组(肝乐冲剂)与对照组(甘力欣胶囊),观察治疗前后各项相关指标的变化及临床疗效。[结果]治疗组疗效指数为93.3%,对照组的66.7%,肝功能改善情况及血清乙肝病毒指标转阴率,治疗组优于对照组,两组比较有显著性差异(P0.05)。[结论]肝乐冲剂治疗慢性乙型肝炎肝郁脾虚型疗效明显。     

多糖铁胶囊治疗妊娠合并缺铁性贫血的临床疗效

目的探讨多糖铁胶囊(商品名:红源达)治疗妊娠合并缺铁性贫血(IDA)的疗效及其安全性。方法采用多中心、临床试验,在广州市内4家医院按入选标准选择480例妊娠合并缺铁性贫血的患者,给予多糖铁胶囊治疗(口服,150mg/次,2次/d),同时服维生素C片0.3g。治疗第2、4和8周后复查血常规和铁代谢,比较治疗前后红细胞(RBC)计数、血红蛋白(Hb)、血清铁蛋白(SF)、血清铁(SI)及可溶性血清铁蛋白受体(sTfR)变化及安全性。结果多糖铁胶囊治疗2周后SF、sTfR较治疗前改善(P<0.05);治疗4周后RBC计数、Hb、SF、sTfR较治疗前显著升高(P<0.05);治疗8周后RBC计数、Hb、SF及sTfR较治疗前升高非常明显(P<0.01),但SI在各检测时间的复查结果差异无统计学意义(P>0.05)。治疗过程中患者均未发生明显的不良发应。结论多糖铁胶囊治疗妊娠合并IDA效果好,无明显不良反应。     

Lymphocytes modulate peritoneal leukocyte recruitment in peritonitis

Objective and design  To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis. Materials or subjects  RAG-1 knockout (KO) mice, NUDE mice and μMT KO mice were compared to their wild-type controls. Treatment  Mice were administered with 1 ml of Brewer’s thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment. Methods  Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array. Results  RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, μMT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines. Conclusions  These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment. J. Hughes and J.-F. Cailhier are joint senior authors.     

Granulocyte-colony stimulating factor promotes liver repair and induces oval cell migration and proliferation in rats

BACKGROUND AND AIMS: Hepatic regeneration is a heterogeneous phenomenon involving several cell populations. Oval cells are considered liver stem cells, a portion of which derive from bone marrow (BM). Recent studies have shown that granulocyte-colony stimulating factor (G-CSF) may be effective in facilitating liver repair. However, it remains unclear if G-CSF acts by mobilizing BM cells, or if it acts locally within the liver microenvironment to facilitate the endogenous restoration program. In the present study, we assessed the involvement of G-CSF during oval cell activation. METHODS: Dipeptidyl-peptidase-IV-deficient female rats received BM transplants from wild-type male donors. Four weeks later, rats were subjected to the 2-acetylaminofluorene/partial hepatectomy model of oval cell-mediated liver regeneration, followed by administration of either nonpegylated G-CSF or pegylated G-CSF. Control animals did not receive further treatments after surgery. The magnitude of oval cell reaction, the entity of BM contribution to liver repopulation, as well as the G-CSF/G-CSF-receptor expression levels were evaluated. In addition, in vitro proliferation and migration assays were performed on freshly isolated oval cells. RESULTS: Oval cells were found to express G-CSF receptor and G-CSF was produced within the regenerating liver. G-CSF administration significantly increased both the magnitude of the oval cell reaction, and the contribution of BM to liver repair. Finally, G-CSF acted as a chemoattractant and a mitogen for oval cells in vitro. CONCLUSIONS: We have shown that G-CSF facilitates hepatic regeneration by increasing the migration of BM-derived progenitors to the liver, as well as enhancing the endogenous oval cell reaction.