N-马来酰-L-缬氨酸酯姜黄素对胃癌MGC-803细胞周期的影响

目的:研究N-马来酰-L-缬氨酸酯姜黄素(MVC)对胃癌MGC-803细胞周期的作用机制。方法:采用MTT法检测MVC对肿瘤细胞的生长抑制作用;采用流式细胞术检测MVC对MGC-803细胞周期的影响;采用Western blot印迹法检测MVC对MGC-803细胞周期相关基因蛋白p21WAF1/CIP1,Cdc 2和Cyclin B1表达的影响。结果:20μmol.L-1 MVC可明显抑制MGC-803细胞生长,可明显诱导MGC-803细胞S,G/M期阻滞。20μmol.L-1MCV作用MGC-803细胞24 h,p21WAF1/CIP1蛋白表达增高,Cdc 2蛋白表达减少。结论:MVC可通过增加细胞周期相关基因p21WAF1/CIP1蛋白的表达和下调Cdc 2蛋白表达,使MGC-803细胞的周期阻断在S,G2/M期,从而抑制MGC-803细胞的生长。     

姜黄素对STS诱导大鼠海马神经元损伤影响

目的 探讨姜黄素对星形孢菌素(STS)诱导的大鼠海马神经元损伤的保护作用。方法 运用乳鼠海马神经元原代培养细胞,采用STS诱导建立神经细胞损伤模型,实验设4组,对照组、模型组、姜黄素组(20 μmol/L)和姜黄素预处理组(姜黄素和STS分别为20 μmol/L),镜下观察神经细胞形态学变化;噻唑蓝(MTT)法测定细胞活性,乳酸脱氢酶(LDH)释放率检测细胞毒性,免疫荧光染色法检测活性氧(ROS)水平;western blot检测细胞中active caspase-3、p-AKT蛋白表达。结果 与模型组比较,姜黄素预处理组神经细胞损伤程度明显减轻;姜黄素预处理组细胞活性(0.877±0.016)较模型组(0.625±0.007)升高(t=3.47,P<0.01),LDH释放量(0.383±0.025)低于模型组(0.582±0.051)(t=3.25,P<0.01);模型组ROS阳性细胞数多于姜黄素预处理组;姜黄素预处理组active caspase-3表达量(0.951±0.089)低于模型组(1.370±0.131)(t=3.64,P<0.01),p-AKT表达量(1.107±0.025)高于模型组(0.611±0.002)(t=5.85,P<0.01)。结论 姜黄素通过抑制细胞ROS释放、下调active caspase-3和上调p-AKT蛋白表达发挥对STS所致大鼠海马神经元损伤的保护作用。     

姜黄素聚合物胶束的制备及体外评价

目的:制备姜黄素的Soluplus聚合物胶束,并对其进行体外评价。方法:采用薄膜分散法制备姜黄素聚合物胶束;采用粒径测定仪、透射电镜、X-射线衍射(XRD)对其进行表征;采用紫外分光光度法测定胶束的包封率和载药量;采用动态膜透析法考察载药胶束的体外释药特性。结果:薄膜分散法制备的胶束呈球形或类球形,平均粒径为(65.54±2.57)nm,平均包封率为(87.73±2.94)%,平均载药量(7.96±2.13)%;XRD结果表明姜黄素以无定型状态或分子状态包载在聚合物胶束中;体外释放结果表明姜黄素的soluplus聚合物胶束具有缓释作用。结论:该胶束制备工艺简单,其粒径、包封率、载药量可控,具有缓释作用。     

姜黄素对5-Fu化疗大鼠肠黏膜屏障保护作用的实验研究

目的：观察姜黄素在利用5-Fu化疗期间对大鼠肠黏膜屏障的保护功能的研究。方法：健康Wister大鼠60只随机分成Control组（20只）,5-Fu组（20只,5-氟尿嘧啶）,5-Fu＋Cur.组（20只,5-氟尿嘧啶＋姜黄素）,在实验的第2、4、6天监测各组大鼠的体重、内毒素、DAO、D-乳酸水平,第7天处死大鼠后取出大鼠回肠,并在光镜下观察肠黏膜组织结构病理变化。结果：与正常组相比,化疗组的体重明显下降（P〈0.05）;内毒素、血浆DAO、D-乳酸水平明显增高（P〈0.05）;肠组织病理学观察可见：肠黏膜明显萎缩,绒毛脱落,并伴有大量炎性细胞浸润。结论：姜黄素对5-Fu化疗期间肠黏膜屏障具有保护作用。     

姜黄素抗大鼠肝星状细胞氧应激脂质过氧化作用的研究

目的:探讨姜黄素(Curcumin,Cur)对大鼠肝星状细胞(hepatic stellate cells,HSC)氧应激所致脂质过氧化的作用.方法:HSC与FeNTA共同培养产生氧应激.以MTT法检测姜黄素对HSC增殖的效应,LDH法检测姜黄素对HSC的毒性作用,以ELISA法测定细胞培养液中Ⅰ型胶原的水平,以试剂盒分别测定细胞培养液中MDA、GSH、GSH-PX、SOD水平.结果:HSC与FeNTA共同培养,细胞内MDA、GSH水平明显升高,GSH-PX、SOD活力明显降低.FeNTA与HSC共同培养,能明显促进HSC增殖和提高细胞培养上清中Ⅰ型胶原的水平.姜黄素可以逆转上述所有FeNTA所致效应.结论:姜黄素抗肝纤维化作用可能与其抗脂质过氧化作用有关.     

Therapeutic Potential of Turmeric in Alzheimer's Disease: Curcumin or Curcuminoids?

Alzheimer's disease (AD) is the most common form of dementia. There is limited choice in modern therapeutics, and drugs available have limited success with multiple side effects in addition to high cost. Hence, newer and alternate treatment options are being explored for effective and safer therapeutic targets to address AD. Turmeric possesses multiple medicinal uses including treatment for AD. Curcuminoids, a mixture of curcumin, demethoxycurcumin, and bisdemethoxycurcumin, are vital constituents of turmeric. It is generally believed that curcumin is the most important constituent of the curcuminoid mixture that contributes to the pharmacological profile of parent curcuminoid mixture or turmeric. A careful literature study reveals that the other two constituents of the curcuminoid mixture also contribute significantly to the effectiveness of curcuminoids in AD. Therefore, it is emphasized in this review that each component of the curcuminoid mixture plays a distinct role in making curcuminoid mixture useful in AD, and hence, the curcuminoid mixture represents turmeric in its medicinal value better than curcumin alone. The progress in understanding the disease etiology demands a multiple‐site‐targeted therapy, and the curcuminoid mixture of all components, each with different merits, makes this mixture more promising in combating the challenging disease. Copyright © 2013 John Wiley & Sons, Ltd.     

Herbal Therapeutics that Block the Oncogenic Kinase PAK1: A Practical Approach towards PAK1‐dependent Diseases and Longevity

Over 35 years research on PAKs, RAC/CDC42(p21)‐activated kinases, comes of age, and in particular PAK1 has been well known to be responsible for a variety of diseases such as cancer (mainly solid tumors), Alzheimer's disease, acquired immune deficiency syndrome and other viral/bacterial infections, inflammatory diseases (asthma and arthritis), diabetes (type 2), neurofibromatosis, tuberous sclerosis, epilepsy, depression, schizophrenia, learning disability, autism, etc. Although several distinct synthetic PAK1‐blockers have been recently developed, no FDA‐approved PAK1 blockers are available on the market as yet. Thus, patients suffering from these PAK1‐dependent diseases have to rely on solely a variety of herbal therapeutics such as propolis and curcumin that block PAK1 without affecting normal cell growth. Furthermore, several recent studies revealed that some of these herbal therapeutics significantly extend the lifespan of nematodes (C. elegans) and fruit flies (Drosophila), and PAK1‐deficient worm lives longer than the wild type. Here, I outline mainly pathological phenotypes of hyper‐activated PAK1 and a list of herbal therapeutics that block PAK1, but cause no side (harmful) effect on healthy people or animals. Copyright © 2013 John Wiley & Sons, Ltd.     

姜黄素对大鼠血瘀性脑缺血模型血液流变学及脑匀浆LD、LDH和TchE水平的影响

目的:探讨姜黄素对血瘀性脑缺血模型的作用特点。方法:采用肌肉注射地塞米松造血瘀模型,给血瘀模型大鼠连续灌服姜黄素混悬液10天,于第11天灌相应药物后1h,大鼠两侧颈总动脉作结扎30min,造脑缺血模型;取血肝素抗凝,测血液流变学;取大鼠脑组织用生理盐水制脑匀浆,测脑匀浆LD、LDH和TchE水平。结果:肌肉注射地塞米松造血瘀模型成功,结扎双侧颈总动脉造脑缺血模型成功;与模型组比,姜黄素可显著降低全血高低切粘度、红细胞聚集指数、全血高低切还原粘度及红细胞刚性,可显著降低脑匀浆乳酸含量,可显著提高脑匀浆乳酸脱氢酶水平及胆碱酯酶水平。结论:姜黄素可显著改善大鼠血瘀性脑缺血模型血液流变学及脑匀浆生化指标。     

姜黄素对过氧化氢诱导氧化损伤的小鼠脑神经瘤细胞的保护作用

<正>姜黄素(curcumin)是从姜科植物Curcumalonga根茎中分离得到的一种脂溶性多酚类化合物,具有抗肿瘤、抗炎症、抗氧化、抗纤维化、保护心血管系统和消化系统等功能[1-2]。近来研究     

Dietary polyphenols and obesity

The prevalence of overweight and obesity and their associated metabolic disorders are considered a major threat to the public's health. While several diet and exercise programs are available for weight loss and prevention of weight regain, progress is often slow and disappointing. Recently, natural bioactive phytochemicals present in foods have been discovered for their potential health benefit effects on the prevention of chronic disorders such as cancer, cardiovascular disease, inflammatory and metabolic diseases including obesity. Polyphenols are a class of naturally-occurring phytochemicals, of which some such as catechins, anthocynines, resveratrol and curcumin have been shown to modulate physiological and molecular pathways that are involved in energy metabolism, adiposity, and obesity. The potential in vivo, beneficial effects of these polyphenols on adiposity and obesity as complementary agents in the up-regulation of energy expenditure have emerged by investigating these compounds in cell cultures, animal models of obesity and in some human clinical and epidemiological studies. In this brief review, the efficacy of the above-named polyphenols and their potential efficacy to modulate obesity and some associated disorders are discussed.