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111.
姜黄素对大鼠癫痫持续状态后海马神经元丢失的影响 总被引:1,自引:0,他引:1
目的:探讨姜黄素对大鼠癫痫持续状态(SE)后海马神经元丢失的影响。方法:Sprague-Dawley(SD)大鼠90只随机均分为癫痫组、姜黄素治疗组和对照组。建立氯化锂-匹罗卡品大鼠SE模型。应用尼氏染色方法动态观察SE后海马CA1区和CA3区存活神经元数量的动态变化。结果:与对照组相比,SE组CA1及CA3区存活神经元数量于SE后2h无明显变化,4h明显减少(P〈0.01),24h和72h后神经元数量减少最为明显;姜黄素组SE后6,24,72hCA3及CA1区存活神经元与对照组相比也有明显减少(P〈0.01),但较SE组多(P〈0.01)。结论:姜黄素对SE后海马神经元具有保护作用。 相似文献
112.
高效液相色谱法测定姜黄通脉胶囊中姜黄素的含量 总被引:1,自引:0,他引:1
目的建立高效液相色谱法测定姜黄通脉胶囊中姜黄素含量的方法。方法采用高效液相色谱法,使用Diamonsil-C18色谱柱,流动相为乙腈-水(用磷酸调节pH值为2.5)(48∶52),流速为1.0mL/min,检测波长为430nm。结果姜黄素在2.675~53.500μg/mL浓度范围内呈良好的线性关系(相关系数r=0.9999),平均回收率为99.78%,相对标准偏差为0.59%。结论本方法简便可靠、分离度较好、结果稳定,可用于姜黄通脉胶囊的质量控制。 相似文献
113.
姜黄素对BGC-823及MCF-7细胞内钙离子信号波动的研究 总被引:1,自引:1,他引:0
研究姜黄素诱导肿瘤细胞凋亡的可能机制 ,利用 fluo-4/AM荧光钙离子探针测定 BGC-82 3细胞与MCF-7细胞经姜黄素处理后的钙波变化 ,以及 BGC-82 3细胞经姜黄素处理后细胞内钙分布的变化。结果表明姜黄素可引起细胞内钙库的动员和钙离子的释放。提示 :细胞内源性钙离子的释放可能是姜黄素诱导细胞凋亡的信号调控机制之一 相似文献
114.
Chearwae W Anuchapreeda S Nandigama K Ambudkar SV Limtrakul P 《Biochemical pharmacology》2004,68(10):2043-2052
P-glycoprotein (Pgp, ABCB1) is an ATP-dependent drug efflux pump linked to development of multidrug resistance (MDR) in cancer cells. Previously [Biochem Pharmacol 2002;64:573-82], we reported that a curcumin mixture could modulate both function and expression of Pgp. This study focuses on the effect of three major curcuminoids--curcumin I, II and III purified from a curcumin mixture--on modulation of Pgp function in a multidrug resistant human cervical carcinoma cell line (KB-V1). The similar IC(50) values for cytotoxicity of curcuminoids of KB-V1, and KB-3-1 (parental drug sensitive cell line) suggest that these curcuminoids may not be substrates for Pgp. Treating the cells with non-toxic doses of curcuminoids increased their sensitivity to vinblastine only in the Pgp expressing drug resistant cell line, KB-V1, and curcumin I retained the drug in KB-V1 cells more effectively than curcumin II and III, respectively. Effects of each curcuminoid on rhodamine123, calcein-AM, and bodipy-FL-vinblastine accumulation confirmed these findings. Curcumin I, II and III increased the accumulation of fluorescent substrates in a dose-dependent manner, and at 15 microM, curcumin I was the most effective. The inhibitory effect in a concentration-dependent manner of curcuminoids on verapamil-stimulated ATPase activity and photoaffinity labeling of Pgp with the [(125)I]-iodoarylazidoprazosin offered additional support; curcumin I was the most potent modulator. Taken together, these results indicate that curcumin I is the most effective MDR modulator among curcuminoids, and may be used in combination with conventional chemotherapeutic drugs to reverse MDR in cancer cells. 相似文献
115.
Detection of curcumin and its metabolites in hepatic tissue and portal blood of patients following oral administration 总被引:6,自引:0,他引:6
Garcea G Jones DJ Singh R Dennison AR Farmer PB Sharma RA Steward WP Gescher AJ Berry DP 《British journal of cancer》2004,90(5):1011-1015
Studies in vitro and in animal models of colorectal and hepatocellular cancers suggest that curcumin is an effective chemopreventive agent. In this pilot trial, we investigated whether oral administration of curcumin results in concentrations of the agent in normal and malignant human liver tissue, which are sufficient to elicit pharmacological activity. In total, 12 patients with hepatic metastases from colorectal cancer received 450-3600 mg of curcumin daily, for 1 week prior to surgery. Levels of curcumin and its metabolites were measured by HPLC in portal and peripheral blood, bile and liver tissue. Curcumin was poorly available, following oral administration, with low nanomolar levels of the parent compound and its glucuronide and sulphate conjugates found in the peripheral or portal circulation. While curcumin was not found in liver tissue, trace levels of products of its metabolic reduction were detected. In patients who had received curcumin, levels of malondialdehyde-DNA (M(1)G) adduct, which reflect oxidative DNA changes, were not decreased in post-treatment normal and malignant liver tissue when compared to pretreatment samples. The results suggest that doses of curcumin required to furnish hepatic levels sufficient to exert pharmacological activity are probably not feasible in humans. 相似文献
116.
Odot J Albert P Carlier A Tarpin M Devy J Madoulet C 《International journal of cancer. Journal international du cancer》2004,111(3):381-387
Curcumin, the active ingredient from the spice turmeric (Curcuma longa Linn), is known to be an anti-oxidant and an anti-inflammatory agent. It has been demonstrated recently to possess anti-angiogenic effects and pro-apoptotic activities against Ehrlich ascites tumor cells. In the current study, curcumin was found to be cytotoxic in vitro for B16-R melanoma cells resistant to doxorubicin either cultivated as monolayers or grown in three-dimensional (3-D) cultures (spheroids). We have demonstrated that the cytotoxic effect observed in the 2 culture types can be related to the induction of programmed cell death. In our in vivo studies, we examined the effectiveness of a prophylactic immune preparation of soluble proteins from B16-R cells, or a treatment with curcumin as soon as tumoral appearance, alone or in combination, on the murine melanoma B16-R. The combination treatment resulted in substantial inhibition of growth of B16-R melanoma, whereas each treatment by itself showed little effect. Moreover, animals receiving the combination therapy exhibited an enhancement of their humoral anti-soluble B16-R protein immune response and a significant increase in their median survival time (> 82.8% vs. 48.6% and 45.7% respectively for the immunized group and the curcumin-treated group). Our study shows that curcumin may provide a valuable tool for the development of a therapeutic combination against the melanoma. 相似文献
117.
Alcohol is a neurotoxin associated with significant morbidity and mortality. Ethanol is found to induce a dose dependent increase in lipid peroxidation (LPO). The elevation in lipid peroxidative products and the loss of antioxidant defense potential are enhanced when alcohol is taken along with polyunsaturated fatty acid (PUFA) or heated PUFA. The present study was undertaken to evaluate the effects of curcumin and photo-irradiated curcumin on alcohol and PUFA induced LPO and lipid pro fi les in plasma. The levels of vitamin C and E were decreased significantly in alcohol + raw as well as heated PUFA groups. The treatment with curcumin and photo-irradiated curcumin (IC) increased their levels significantly. The increase was more significant in the IC group than the curcumin group. The levels of cholesterol, phospholipids (PL), triglycerides (TG), free fatty acids (FFA), thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HP) were increased significantly in alcohol + raw as well as heated PUFA groups and the treatment with curcumin and IC, brought back the levels. But the IC reduced the levels more significantly than curcumin. Thus, our results indicate that IC is a more potent antioxidant than curcumin. 相似文献
118.
本文对姜黄药材中总姜黄素的提取纯化方法进行了较深入的实验研究,采用可见-紫外分光光度法测定提取物中总姜黄素的含量。并对超声辅助提取法做了考察,采用正交实验法对超声提取法提取工艺条件进行优选,得到最佳的提取工艺条件为:姜黄粉碎成40目,75%的乙醇超声提取,提取时间为60min.,姜黄素的提取得率为4.96%。 相似文献
119.
Nidhi Bharal Agarwal Seema Jain Dheeraj Nagpal Nitin Kumar Agarwal Pramod K. Mediratta Krishna K. Sharma 《Fundamental & clinical pharmacology》2013,27(2):169-172
Contemporary research indicates promising anticonvulsant effect of curcumin. However, its poor oral bioavailability is a major hindrance toward its pharmacological action. Thus, this study was carried out to evaluate the acute effect of liposome‐entrapped curcumin on increasing current electroshock seizures (ICES) test, pentylenetetrazole (PTZ)‐induced seizures, and status epilepticus in mice. Liposome‐entrapped curcumin in doses 25 and 50 mg/kg demonstrated significant increase in seizure threshold current and latency to myoclonic and generalized seizures in ICES test and PTZ‐induced seizures, respectively. Similarly, liposomal‐entrapped curcumin also increased the latency to the onset and decreased the duration of seizures during status epilepticus in mice. To conclude, liposomal‐entrapped curcumin possesses anticonvulsant activity against status epilepticus in mice. 相似文献
120.
姜黄素是一种提取自姜黄的多酚类化合物,具有神经保护作用,可以通过血脑屏障,是治疗多种神经系统疾病候选的理想药物。在胶质瘤、脑血管病、癫痫、神经退行性疾病和创伤性脑损伤等神经系统疾病中的作用已受到广泛关注。然而,姜黄素因生物利用率低,导致应用受到限制。近年来,人们发现纳米颗粒可有效提高姜黄素生物利用率。众多前期临床试验中发现,纳米颗粒药物输送系统使姜黄素药理作用得到了良好的应用。因此,本文就姜黄素纳米颗粒对中枢神经系统各类疾病的作用及机制的最新研究进展进行综述,以期为姜黄素在神经系统的广泛应用提供参考。 相似文献