自拟前列汤对大鼠前列腺增生模型b-FGF表达影响的实验研究

目的:观察自拟前列汤对前列腺增生模型大鼠b-FGF表达的影响以及对前列腺湿重、前列腺指数的改变.方法:选用SD大鼠60只,给于去势加皮下注射丙酸睾丸酮,2周后分别给于相应药物灌胃,6周后再称其体重,断颈处死,取其前列腺组织,称其湿重,计算前列腺指数,并用免疫组化测b-FGF,所得数据进行比较.结果:3个治疗组的前列腺湿重、前列腺指数、及bFGF的表达明显低于模型对照组.结论:自拟前列汤可以明显抑制模型大鼠的前列腺增生,其机制可能是通过减少b-FGF的表达来实现的.     

角膜营养不良家系患病角膜细胞角蛋白及b-FGF与TGF-b1表达的研究

目的 了解深圳地区-角膜营养不良家系中患病角膜中细胞角蛋白,纤维组织相关的碱性成纤维细胞因子(b-FGF)和转化生长因子(TGF-b1)的表达改变.方法 分别按照SABC免疫组织化学方法及双夹心ELISA方法检测家系中5例受累颗粒状营养不良角膜及1例格子状角膜营养不良角膜中高、低分子量细胞角蛋白的分布表达情况,以及b-FGF和TGF-bl的表达,散发4例格子状角膜营养不良角膜及4例意外死亡者的正常角膜做为对照.结果 高、低分子量细胞角蛋白在本角膜营养不良家系患者的角膜中分别有100%及66.7%的阳性表达(阳性/弱阳性均计入阳性表达率),散发性格子状营养不良角膜均有弱阳性的表达,正常角膜则没有表达.b-FGF在各组角膜中的表达无明显差异.TGF的含量在家系颗粒状角膜营养不良的角膜中平均为1.225±0.867pg/ml,与正常角膜中的含量无明显差异;家系格子状角膜营养不良的角膜中为4.0pg/ml,比正常角膜明显增多(t检验t=36.373,P=0.017);散发格子状角膜营养不良的角膜中平均为(8.375±0.667)pg/ml,比正常角膜明显增多(t检验t=9.413,P=0.020);正常角膜中TGF的含量平均为(0.0625±0.0088)pg/ml.结论 细胞角蛋白在本家系颗粒状角膜营养不良及格子状角膜营养不良均有表达,高分子量细胞角蛋白的表达更明显,其发病与角膜上皮表达细胞角蛋白的增多有关联.家系中的主要角膜营养不良类型(颗粒型)的患病角膜组织中TGF-bl及b-FGF的含量均无明显改变.格子状角膜营养不良(包括家系及散发)的角膜组织中TGF-b1的含量明显增加,其发病与板层纤维增生有一定的关联.

Abstract：

Objective To understand the expression changes of the sick corneal keratin and b-FGF and TGF-b1 in a corneal dystrophy family in Shenzhen. Methods The expression of the high and low molecular weight distribution of cytokeratin were detected by SABC immunohistochemistry,and the b-FGF and TGF-b1 by double-sandwich ELISA method in 5 family granular cases and one lattice case of corneal dystrophy, 4 distribute lattice corneal dystrophy cases and 4 normal cornea as control. Results 100% and 66.7% of positive expression of high and low molecular weight cytokeratin in the family cases, respectively, and distributed cases were weak positive, and the normal ones were negative. The b-FGF expression was not different in all the cases. Was the TGF expression higher in family and distributed lattice corneal dystrophy cases only, with 4.0pg/ml (r=36.373, P=0.017) and 8.375 ± 0.667pg/ml (t=9.413, P=0.020), not the family granular cases of 1.225±0.867pg/ml, than the normal of 0.0625±0.0088 pg/ml. Conclusions The cytokeratins is expressed higher in family granular and lattice corneal dystrophy cases, high molecular weight cytokeratin especially, and associate with the pathogenesis. The family granular cases are not different for TGF-b1 and b-FGF of the corneal tissue. TGF-b1 increases significantly in the lattice corneal dystrophy cases including family and dissemination, and associate with the pathogenesis.     

b-FGF对Ⅱ度烧伤创面愈合的影响

目的:观察b-FGF在Ⅱ度烧伤治疗中对创面愈合的影响。方法:全组80人,使用b-FGF47人,对照组33人,其它治疗方法相同,比较创面愈合速度。结果:使用b-FGF组创面愈合速度明显增快。结论:在常规治疗烧伤的基础上,局部使用b-FGF,可以加速创面愈合,不失为一种值得推进方法。     

Long-term follow-up of tracheal cartilage growth promotion by intratracheal injection of basic fibroblast growth factor

Background

Intratracheal injection of basic fibroblast growth factor (b-FGF) has been shown to enlarge the tracheal lumen 4?weeks after treatment. The objective of this study was to investigate the long-term effect of tracheal cartilage growth promotion by intratracheal injection of b-FGF.

TGFβ1和b-FGF在肾血管性高血压大鼠血管重塑中的作用

目的 探讨碱性成纤维细胞生长因子(b-FGF)和转化生长因子β1(TGF-β1)在肾血管性高血压大鼠血管重塑中的作用机制.方法 将8周龄健康雄性wistar大鼠40只随机分为对照组和模型组,每组各20只,按照“二肾一夹”法制备高血压大鼠模型.每周检测大鼠尾动脉收缩压1次.8周后处死取颈总动脉、腹主动脉做形态学观察,免疫组织化学法检测动脉管壁中b-FGF和TGF-β1的蛋白表达.结果 与对照组相比,模型组尾动脉收缩压增高[(149.76±7.56)mm Hg比(88.68±3.96)mm Hg,P＜0.01];动脉中膜增厚(P＜0.05);管壁b-FGF、TGF-β1表达增强(P＜0.01);b-FGF与TGF-β1表达呈正相关,在颈总动脉r=0.88,在腹主动脉r=0.87,均P＜0.01.结论 b-FGF、TGF-β1可能促进了高血压血管重塑的病理过程,二者间可能存在相互作用.     

碱性成纤维细胞生长因子与肾癌新血管形成的关系

b FGF最初从大脑和垂体中提取 ,能够刺激成纤维细胞有丝分裂。在肾癌 ,bFGF、bFGF受体、bFGFMRNA均在癌组织在表达 ,也能够在病人血、尿中测到 ,且随肿瘤的分级、分期升高而升高。它促进肾癌组织新血管形成 ,加速肾癌增殖和转移。其他生长因子和某些药物可能上调或下调bFGF表达     

Relationship of PARG with PARP, VEGF and b-FGF in colorectal carcinoma

Objective: To investigate the relationship of poly(ADP-ribose)glycohydrolase(PARG) with poly (ADP-ribose) polymerase(PARP), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor(b-FGF) in colorectal carcinoma(CRC).Methods: Immunohistochemical analysis was used to detect PARG, PARP, VEGF and b-FGF in human colorectal carcinoma. Flow cytometry was used to detect PARG and PARP in murine CT26 cell line. Gallotannin (GLTN) was served as PARG inhibitor. Results: The individual positive rates of PARG, PARP, VEGF and b-FGF were 55.81%(24/43), 97.67%(42/43), 79.07%(34/43) and 81.40%(35/43), respectively, which were significantly higher than those of control group. The positive PARG was correlated to PARP(r=0.3703, P＜0.05) and b-FGF (r=0.4838, P＜0.05). The positive PARP was correlated to VEGF (r=0.3968, P＜0.05) and b-FGF (r=0.5610, P＜0.05). Both PARG and PARP were expressed in CT26 cells. The positive staining rates of PARG and PARP in GLTN-treated group were 7.3% and 52.38%, respectively. They were markedly reduced than those of control group (55.41% and 95.28%, P＜0.01, n=10000).Conclusion: The data suggest that PARG expression probably plays a role for VEGF and b-FGF expression in colorectal carcinoma.     

Serum VEGF and b-FGF profiles after tension-free or conventional hernioplasty

Background Angiogenesis is strongly influenced by vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF), whose production is also regulated by interferon (IFN)- and interleukin (IL)-10. The aim of this study was to evaluate the modifications of serum VEGF, b-FGF, IFN- and IL-10 levels in patients with inguinal hernia undergoing hernioplasty with the Lichtenstein technique (LH) using polypropylene mesh or with Bassini open conventional inguinal hernia repair (BH).Materials and methods Randomly, 16 patients underwent BH, and 16 were treated with the LH technique using polypropylene mesh. Blood samples were collected 24 h prior to surgery and then 6, 24, 48 and 168 h postoperatively. The serum concentrations of VEGF, b-FGF, IFN- and IL-10 were evaluated.Results In BH patients, a peak of VEGF synthesis at 6 h with a normalization of this parameter 24 h after surgery has been observed. In the same subjects, b-FGF synthesis increased after surgery reaching significant levels 48 h later. On the contrary, in LH patients, a decrease in the serum VEGF and b-FGF concentrations was detected after surgery and their increase afterwards. IL-10 was increased in both groups 6 h after operation and declined to preoperative levels 24 h afterwards. IFN- enhanced in LH patients 6 h after surgery, whereas no modifications were detected in BH subjects.Conclusions This preliminary study shows that VEGF and b-FGF modifications, associated with alterations of cytokine secretion, are detectable in human undergoing hernioplasty, and suggests that they could somehow influence in the wound-healing process.     

Inhibitory effects of pioglitazone on alkaline burninduced corneal neovascularization in rats

AIM: To investigate the effects of pioglitazone on corneal neovascularization（CNV）induced by alkaline burn in rats and on expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF). ·METHODS: CNV was induced by alkaline burn in rats which were then administered pioglitazone subconjunctivally. Corneal angiogenesis was evaluated and observed with a slit lamp microscope and the VEGF and b-FGF level were determined by immunohistochemistry and Western blot at different intervals. ·RESULTS: The CNV of the rats was shown two days after alkaline burn, and reached its highest level in 7-10 days. The mean values of the area and length of neovascularization and the VEGF and b-FGF levels in the pioglitazone-treated group were significantly lower than those in the control group at various time. · CONCLUSION: Local application of pioglitazone could remarkably inhibit CNV, probably by means of downregulation of VEGF and b-FGF expression.     

万托林与普米克对慢性阻塞性肺疾病气道重塑患者血清血管内皮生长因子、神经生长因子、碱性成纤维细胞生长因子的影响

目的分析研究万托林与普米克对慢性阻塞性肺疾病（COPD）气道重塑患者血清血管内皮生长因子（VEGF）、神经生长因子（NGF）、碱性成纤维细胞生长因子（b-FGF）的影响。方法选择我院2009年1月～2011年6月收治的110例COPD气道重塑患者,随机分为A组（万托林治疗组）和B组（普米克治疗组）,每组各55例。所有患者均采用综合疗法,包括合理搭配营养、低流量给氧、祛痰、局部雾化、舒适护理等。在此基础上,A组患者给予万托林吸入治疗（200μg,2次／d）;B组患者给予普米克吸入治疗（200μg,2次／d）。对比分析两组患者的VEGF、NGF、b-FGF水平。结果经治疗,所有患者VEGF、NGF、b—FGF水平得到有效的控制（t=5．14、4．92、4．51,P〈0．05）。B组VEGF、NGF、b—FGF水平明显低于A组（t=4．29、4．12、3．84,P〈0．05）。结论万托林、普米克均能显著改善COPD患者的气道重塑作用,调节患者的VEGF、NGF、b—FGF水平。普米克的临床疗效较万托林更为确切,值得在临床推广。