先天性胫骨假关节中碱性成纤维细胞生长因子（b—FGF）的免疫组化研究

目的：明确碱性成纤维细胞生长因子（b-FGF)在先天性胫肌假关节（CPT）骨膜中的表达水平，探讨b-FGF在CPT发病中的作用，方法：采用免疫组化方法对21例CPT病人的25个病变骨膜标本中b-FGF的表达进行检测。以正常骨膜（10例），创伤性假关节（TP）骨膜（20例）和纤维瘤病组织（10例）作为对照，结果：所有正常骨膜，创伤性假关节标本b-FGF染色均阴性，15例先天胫骨假关节骨膜和6例纤维瘤病病变组织中b-FGF染色阳性，两者无显著性差异（P＞0．05），正常骨膜及创伤性假关节（TP）骨膜中b-FGF表达水平与先天胫骨假关节骨膜和纤维病病变组织有显著性差异（P＜0．01），结论：b-FGF在先天性胫骨假关节（CPT）发病中起了重要作用。     

Human amniotic fluid stem cells support undifferentiated propagation and pluripotency of human embryonic stem cell without b-FGF in a density dependent manner

Human embryonic stem cells (hESCs) are pluripotent cells which can give rise to almost all adult cell lineages. Culture system of hESCs is complex, requiring exogenous b-FGF and feeder cell layer. Human mesenchymal stem cells (MSCs) not only synthesize soluble cytokines or factors such as b-FGF, but also provide other mechanism which might play positive role on sustaining hESCs propagation and pluripotency. Human amniotic fluid stem (AFS) cells, which share characteristics of both embryonic and adult stem cells, have been regarded as promising cells for regenerative medicine. Taking advantage by AFS cells, we studied the ability of AFS cells in supporting undifferentiated propagation and pluripotency of Chinese population derived X-01 hESCs. Human AF-type amniotic fluid stem cells (hAF-AFSCs) transcribed genes including Activin A, TGF-β1, Noggin and b-FGF, which involved in maintaining pluripotency and self-renewal of hESCs. Compared to mouse embryonic fibroblasts (MEFs), hAF-AFSCs secreted higher concentration of b-FGF which was important in hESCs culture (P < 0.05). The hESCs were propagated more than 30 passages on hAF-AFSCs layer with exogenous b-FGF supplementation, keeping undifferentiated status. While exogenous b-FGF was obviated, propagation of hESCs with undifferentiated status was dependent on density of hAF-AFSC feeder layer. Lower density of hAF-AFSCs resulted in rapid decline in undifferentiated clone number, while higher ones hindered the growth of colonies. The most appropriate hAF-AFSCs feeder density to maintain the X-01 hESC line without exogenous b-FGF was 15-20×10⁴/well. To the best of our knowledge, this is the first study demonstrating that hAF-AFSCs could support undifferentiated propagation and pluripotency of Chinese population derived hESCs without exogenous b-FGF supplementation.     

Comparing FK-506 with Basic Fibroblast Growth Factor (b-FGF) on the Repair of a Peripheral Nerve Defect Using an Autogenous Vein Bridge Model

ABSTRACT?

Objective: The limited availability of donor sites for nerve grafts and the morbidity associated with their harvesting serve as motivating factors to actively conduct research to find alternatives to the status quo. Experimental and clinical studies have shown that a vein segment used to bridge a peripheral nerve defect leads to a functional nerve repair. Both FK-506 and b-FGF have been reported to enhance peripheral nerve regeneration. This study compared the effects of FK-506 with that of b-FGF on peripheral nerve regeneration in a rat autogenous vein graft conduit model. Methods: The main trunk of the right sciatic nerve was transected and bridged by an autogenous vein in 30 rats. Small osmotic pumps were placed just proximal to the anastomoses. Groups of 10 rats were assigned to receive saline solution, b-FGF (2,000 units), or FK-506 (0.5 mg/kg/day) via the osmotic pumps for 2 weeks. Sciatic nerve regeneration was evaluated by sensory function, walking track analysis, electrophysiologic studies, and light microscopic evaluation. Results: On post-operative day 90, there was a statistically significant difference (p <.005) in nerve regeneration between the rats who received saline compared with those who received FK-506 or b-FGF. This was determined using sensory function tests, sciatic function index, and electrophysiologic studies. The number of nerve axons, as determined by histological analysis, revealed there were significantly more nerve fibers which were regenerated in both experimental groups (FK-506 and b-FGF) when compared with rats who received saline. There was no statistically significant difference in the number of nerve axons that regenerated in rats injected with FK-506 vs. rats injected with b-FGF. Conclusion: FK-506 and b-FGF promote similar nerve regeneration in rats compared with control.     

Promotion of tracheal cartilage growth by intra-tracheal injection of basic fibroblast growth factor (b-FGF)

Purpose

Basic fibroblast growth factor (b-FGF) is a very effective growth factor that induces the proliferation of chondrocytes. This study aimed to investigate whether intra-tracheally-injected b-FGF solution promotes the growth of tracheal cartilage.

Methods

Group 1: 500 μl of distilled water was injected at the posterior wall of the cervical trachea of New Zealand white rabbits by using a tracheoscope (n = 5). Group 2: 100 μg/500 μl of b-FGF solution was injected at the posterior wall of the cervical trachea (n = 5). Group 3: Biodegradable gelatin hydrogel microspheres incorporating 100 μg/500 μl of b-FGF solution were injected at the posterior wall of the cervical trachea (n = 5). All animals were sacrificed 4 weeks later, and the outer diameter and luminal area of the cervical trachea at the site of b-FGF injection were measured.

Results

The cervical tracheas in the two b-FGF injection groups were spindle-shaped and had a maximum diameter at the injection site. The median outer diameter of the cervical trachea in Groups 1, 2, and 3 was 7.3, 8.0, and 8.0 mm, respectively, showing a significant difference among Groups 1, 2, and 3 (P = 0.04). The median luminal area in Groups 1, 2, and 3 was 27.4, 29.4, and 32.1 mm², respectively. The ad hoc test showed a marginally significant difference only between groups 1 and 3 (p = 0.056).

Conclusion

Intra-tracheal injection of slowly released b-FGF enlarged the tracheal lumen.     

严重心脏病手术切口脂肪液化护理

目的对严重心脏病手术切口脂肪液化进行早期专业护理。方法对危重、老年、糖尿病、慢性病及营养不良患者术后可能出现的切口脂肪液化提前介入护理,脂肪液化切口早期使用b-FGF(外用重组人碱性成纤维细胞生长因子)处理创面。结果对13例脂肪液化患者进行临床观察后证实,b-FGF可促进心脏手术切口脂肪液化的愈合,无延期溃烂,减轻护理工作量。     

纤维生长因子在牛晶状体上皮细胞中的表达

目的:晶状体上皮细胞产生纤维生长因子并表达纤维生长因子的受体。本实验的研究目的是调查和研究晶状体上皮细胞增生调节和发育的规律。方法:通过使用牛晶状体上皮细胞培养后,使用“创口愈合方法”检查晶状体上皮细胞的增生和移动,用刮胡刀片在培养的牛晶状体上皮细胞上做一单层晶状体上皮细胞,然后加入一定介质观察和测量晶状体上皮细胞的数量、DNA合成及PCR方法检测基因产量。结果:suramin, protamine和抗纤维生长因子抗体抑制晶状体上皮细胞移动,晶状体上皮细胞的DNA合成也受到了抑制。结论:晶状体组织存在着纤维生长因子的调节系统,而且,这种调节系统在晶状体上皮细胞的增生和分化中起到重要的作用。     

麻红止哮合剂对哮喘气道重塑大鼠血管内皮生长因子与碱性成纤维细胞生长因子浓度的影响

目的观察麻红止哮合剂对与哮喘气道重塑密切相关的血管内皮生长因子(VEGF)与碱性成纤维细胞生长因子(b-FGF)的影响,探讨其治疗哮喘慢性持续期的作用途径。方法SD大鼠40只,随机分为正常组、造模组、激素组和中药组。以腹腔注射100g/L卵蛋白(OVA)1mL和10g/L的OVA吸入激发制成支气管哮喘模型。激素组和中药组在每次雾化前20min给予强的松或中药干预。空白组和造模组在每次雾化前20min给予2mL生理盐水。用ELISA法测定血清VEGF与b-FGF浓度。结果造模组血清VEGF与b-FGF浓度较正常组、激素组和中药组明显增加(P<0.05)。正常组、激素组和中药组组间比较,血清VEGF浓度有显著差异(P<0.05);正常组、激素组和中药组组间比较,血清b-FGF浓度无显著性差异(P>0.05)。结论麻红止哮合剂治疗哮喘慢性持续期的作用途径,可能有一部分是通过降低血清VEGF、b-FGF浓度来延缓支气管哮喘气道重塑的形成,具有和强的松相似的效果。     

罗达灵通胶囊对前列腺增生大鼠PCNA与b-FGF的影响

目的 探讨罗达灵通胶囊对PCNA（增殖细胞核抗原，proliferating cell nuclear antigen）与b-FGF（碱性成纤维细胞生长因子，base fibroblast growth factor）在前列腺增生大鼠中表达的影响及其相关性。方法 采用去势大鼠注射丙酸睾酮的方法复制前列腺增生模型，采用灌胃的方式给予大鼠罗达灵通胶囊高、低剂量及等体积去离子水，连续15 d后处死，观察前列腺组织形态，并通过免疫组化的方法检测PCNA和b-FGF的表达情况，进行相关性分析。结果 罗达灵通胶囊对大鼠的b-FGF表达水平与PCNA指数有影响，且两者之间存在相关性（r＝0．8781，P＜0．005）。结论 b-FGF和PCNA在前列腺增生的过程中均有一定作用。     

氟伐他汀对SD大鼠高脂血症肾脏的保护作用

目的观察SD大鼠高脂血症肾组织血管细胞黏附分子1（VCAM-1）和碱性成纤维细胞生长因子（bFGF）的表达以及氟伐他汀的干预。方法24只健康雄性SD大鼠随机分为,空白对照组（A组）、高脂饮食组（B组）、氟伐他汀组（C组）,每组8只。10周后,采用BackmaanLX 20全自动生化分析仪测定血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL）的浓度;HE染色观察肾组织病理改变,并用免疫组化方法测定VCAM-1和bFGF的表达。结果同高脂饮食组相比,治疗组VCAM-1和bFGF在肾组织中的表达明显降低。结论高脂血症时,肾组织VCAM-1和bFGF表达增强;氟伐他汀可通过降低VCAM-1和bFGF在肾组织中的表达,减轻肾组织的炎症反应,抑制肾组织纤维化,从而具有延缓慢性肾衰竭进展的作用。     

剖宫产术后腹壁切口脂肪液化的防治

目的:探讨剖宫产术后腹壁切口脂肪液化的病因及防治。方法:2001年1月-2006年8月79例剖宫产术后腹壁切口脂肪液化患者以两种不同方法进行治疗,将患者分为对照组和观察组,观察两组在切口愈合时间及有无疤痕方面的差别。结果:对照组患者切口于脂肪液化后(13±3)d愈合,17例出现疤痕;观察组切口于脂肪液化后(6±2)d愈合,3例出现疤痕,两组差异有统计学意义(P<0.01)。结论:正确的治疗方法可缩短切口愈合时间且较少出现疤痕。