乙肝转阴汤1、2号的制备及应用

目的 :观察院内自制制剂乙肝转阴汤1、2号抗乙肝病毒、改善肝功能的疗效。方法 :选择慢性乙型肝炎患者100例 ,随机分成两组 ,治疗组用乙肝转阴汤1、2号 (150ml ,bid ,po) ,对照组口服保护肝功能药物 ,疗程均为6个月。结果 :治疗组谷丙转氨酶明显下降 ,HBeAg、HBV -DNA阴转率分别为44 7 %、42 1 % ,与对照组比较有显著性差异 (P<0.05)。结论 :乙肝转阴汤1、2号具有良好的抗病毒、改善肝功能的作用。     

感停抗腺病毒的实验研究

目的:探讨感停的抗病毒作用疗效。方法:采用微量细胞培养法进行抗病毒疗效和病毒滴定。结果:感停对腺病毒的最小治疗浓度为:6.5μg/ml,感停对腺病毒的治疗指数(therapeutic index,TI)等于5,经最小有效剂量感停作用后,腺病毒的滴度由105TCID50/0.1ml下降为103.5TCID50/0.1ml。结论:感停在体外对腺病毒有一定的治疗作用。     

青果中氨基酸成分的测定

用日立835氨基酸自动分析仪测定了青果中氨基酸的种类和含量,共计18种,其中包括其它植物中较少有的牛磺酸.氨基酸总量为4.45mg/g生药.     

Construction of Eukaryotic Expression Vector with mBD1-mBD3 Fusion Genes and Exploring Its Activity against Influenza A Virus

Influenza (flu) pandemics have exhibited a great threat to human health throughout history. With the emergence of drug-resistant strains of influenza A virus (IAV), it is necessary to look for new agents for treatment and transmission prevention of the flu. Defensins are small (2–6 kDa) cationic peptides known for their broad-spectrum antimicrobial activity. Beta-defensins (β-defensins) are mainly produced by barrier epithelial cells and play an important role in attacking microbe invasion by epithelium. In this study, we focused on the anti-influenza A virus activity of mouse β-defensin 1 (mBD1) and β defensin-3 (mBD3) by synthesizing their fusion peptide with standard recombinant methods. The eukaryotic expression vectors pcDNA3.1(+)/mBD1-mBD3 were constructed successfully by overlap-PCR and transfected into Madin-Darby canine kidney (MDCK) cells. The MDCK cells transfected by pcDNA3.1(+)/mBD1-mBD3 were obtained by G₄₁₈ screening, and the mBD1-mBD3 stable expression pattern was confirmed in MDCK cells by RT-PCR and immunofluorescence assay. The acquired stable transfected MDCK cells were infected with IAV (A/PR/8/34, H1N1, 0.1 MOI) subsequently and the virus titers in cell culture supernatants were analyzed by TCID₅₀ 72 h later. The TCID₅₀ titer of the experimental group was clearly lower than that of the control group (p < 0.001). Furthermore, BALB/C mice were injected with liposome-encapsulated pcDNA3.1(+)/mBD1-mBD3 through muscle and then challenged with the A/PR/8/34 virus. Results showed the survival rate of 100% and lung index inhibitory rate of 32.6% in pcDNA3.1(+)/mBD1-mBD3group; the TCID₅₀ titer of lung homogenates was clearly lower than that of the control group (p < 0.001). This study demonstrates that mBD1-mBD3 expressed by the recombinant plasmid pcDNA3.1(+)/mBD1-mBD3 could inhibit influenza A virus replication both in vitro and in vivo. These observations suggested that the recombinant mBD1-mBD3 might be developed into an agent for influenza prevention and treatment.     

有机硒潜在药物的研究进展

有机硒药物是一类新型化合物,由于其具有抗病毒、抗肿瘤以及治疗神经系统方面疾病的作用,已成为药物研究的热点。研究表明,有机硒类化合物还具有抗炎、抗衰老、防治心血管疾病及预防肝部疾病等药理作用。由于硒具有独特的生化性质和药理作用,因此开发含硒类药物极具意义。该文就近年来有机硒潜在药物的研究情况进行综述。     

利咽喷剂抗流感及腺病毒实验研究

目的探讨利咽喷剂对甲、乙型流感病毒及腺病毒4型的抗病毒作用。方法采用固定药液稀释病毒进行鸡胚尿囊腔注射法及单层细胞病变抑制法对其进行抗病毒实验。结果①甲、乙两型流感病毒对照组半数感染量(EID50)的差数均大于5,反对数大于50;②对甲、乙型流感病毒及腺病毒4型的抑制指数(TI)分别为2、2、1.9。结论利咽喷剂对甲型流感病毒,乙型流感病毒,腺病毒4型均有不同程度的抑制作用。     

龙牙楤木总甙抗病毒作用研究

结果表明龙牙木总甙具有明显的抗脊髓灰质炎病毒Ⅲ型、柯萨奇病毒Ｂ＿３和Ａ￣（１６）、单纯疱疹病毒Ⅰ型、腺病毒Ⅲ型、埃可病毒６型的活性。表现为能显著抑制细胞病变的发生，使组织培养的细胞得到保护。     

医院信息系统中数据备份和安全策略设计与实现

本文针对目前多数综合性医院HIS系统中所面对的数据备份与安全策略的问题 ,结合医院的实际情况 ,设计出了一套既实用又可靠的策略 ,并阐述了具体的设计与实现方法。     

海南省107岁高龄奥密克戎变异株BA.5.1.3感染新型冠状病毒肺炎1例

目的 总结1例海南高龄感染新型冠状病毒奥密克戎变异株BA.5.1.3的新型冠状病毒肺炎（简称新冠肺炎）患者的临床特征和诊疗经验。方法 回顾分析2022年8月15日海口定点医院收治的1例高龄新冠病毒Omicron变异株BA.5.1.3感染患者的临床资料及治疗经过。结果 患者女性,107岁,因“发热、咳嗽1 d”入院。其2位家属感染新冠病毒。患者病初出现发热,伴咳嗽、咳痰,咳少许白色粘痰,伴咽痛、肌肉酸痛、乏力。咽拭子核酸检测阳性,为Omicron变异株BA.5.1.3感染。确诊为新型冠状病毒肺炎轻型,给予抗病毒、中药调理、抗凝、调节电解质紊乱及对症等治疗9 d,患者临床症状缓解,2次核酸检测阴性后治愈出院。1周后随访患者恢复情况良好。结论 Omicron变异株BA.5.1.3感染的新冠肺炎的传染性强,给予抗病毒、中药调理等综合治疗取得较好的疗效。对于高龄患者,要注意维持各器官功能和内环境稳定,有助于改善患者预后。     

CD4+ T cells are important for clearance of DA strain of TMEV from the central nervous system of SJL/J mice

DA and other TO subgroup strains of Theiler's murine encephalomyelitis virus synthesize a protein called L* which interferes with DA virus clearance from the central nervous system of susceptible SJL mice, thereby fostering virus persistence and a late demyelinating disease. By using CD4 (-/-) and CD8 (-/-) mice as well as adoptive transfer studies we demonstrate that susceptible mouse strains are capable of clearing DA virus through a CD4+ T cell-dependent activity that is inhibited by L*.