Crystal-Deficient Alveolar Soft Part Sarcoma

Classically, ultrastructural examination of alveolar soft part sarcoma reveals large, dramatic, rhomboid to needlelike crystals with a characteristic substructure. In this study of four cases of alveolar soft part sarcoma, only two exhibited large crystals, which were rare. All four cases, however, exhibited round, electron-dense granules, and in the two cases without large crystals these granules rarely exhibited elongation with the characteristic substructure of alveolar soft part sarcoma that permits definitive diagnosis. Two of these cases had been previously studied at other institutions, where crystals were not identified ultrastructurally and electron microscopy was considered noncontributory. Large crystals, then, may be rare or absent in alveolar soft part sarcoma. Careful search may be necessary to find granules with the characteristic periodic substructure.     

腓肠神经营养血管皮瓣修复足背皮肤软组织缺损

目的探讨腓肠神经营养血管皮瓣修复足背皮肤软组织缺损的疗效及其适应证。方法21例腓肠神经营养血管皮瓣转位修复足背皮肤软组织缺损,其中5例皮瓣远端达到跖趾关节,皮瓣面积(6cm×4cm)～(15cm×13cm)。结果21例皮瓣中,16例皮瓣远端在跖趾关节以近者完全成活;5例皮瓣远端达到或者超过跖趾关节者有3例皮瓣远端部分坏死,2例皮瓣远端小隐静脉与足背静脉吻合者完全成活。结论腓肠神经营养血管皮瓣是一种修复足背皮肤软组织缺损的较理想的手术方法,其修复创面的最远端可达到跖趾关节。     

Analysis of Connective Tissue Progenitor Cell Behavior on Polydimethylsiloxane Smooth and Channel Micro-Textures

Growth of human connective tissue progenitor cells (CTPs) was characterized on smooth and microtextured polydimethylsiloxane (PDMS) surfaces. Human bone marrow derived cells were cultured for nine days under conditions promoting osteoblastic differentiation on Smooth PDMS and PDMS Channel microtextures (11 m high, 45 m wide channels, and separated by 5 m wide ridges). Glass tissue culture dish surfaces were used as controls. Cell numbers per colony, cell density within colonies, alignment of cells, area of colonies, and colony shapes were determined as a function of substrate surface topography. An alkaline phosphatase stain was used as a marker for osteoblastic phenotype. CTPs attached, proliferated, and differentiated on all surfaces with cell process lengths of up to 80 m. Cells on the Smooth PDMS and control surfaces spread and proliferated as colonies in proximity to other cells and migrated in random directions creating colonies that covered significantly larger areas (0.96 and 1.05 mm², respectively) than colonies formed on PDMS Channel textures (0.64 mm²). In contrast, cells on PDMS Channel textures spread, proliferated, aligned along the channel axis, and created colonies that were more dense, and with lengths of longest colony axes that were significantly longer (3252 m) than those on the Smooth PDMS (1265 m) and control surfaces (1319 m). Cells on PDMS Channel textures were aligned at an angle of 14.44° relative to the channel axis, and the resulting colonies exhibited a significantly higher aspect ratio (13.72) compared to Smooth PDMS (1.57) and control surfaces (1.51).     

Intramuscular dendritic fibromyxolipoma: Myxoid variant of spindle cell lipoma?

Dendritic fibromyxolipoma (DFML) is an uncommon, recently described, benign soft tissue lesion that shares many clinical and pathological features with myxoid variants of spindle cell lipoma (SCL). As described, DFML is distinguished from SCL by the presence of dendritic cytoplasmic processes, abundant keloidal collagen and a prominent, often plexiform vascular pattern. We describe the first known reported case of an intramuscular DFML that occurred in the right shoulder region of a 73-year-old man. The tumor displayed the typical histopathological features of DFML but also included foci of chondroid metaplasia, a previously unreported finding. This report also discusses the differential diagnosis, particularly distinguishing DFML from SCL and myxoid liposarcoma. In view of the similarities in many clinical and pathological features between SCL and DFML, we speculate that DFML probably represents an unusual variant of myxoid SCL.     

Subgrouping and grading of soft-tissue sarcomas by fine-needle aspiration cytology: a histopathologic correlation study

To evaluate the accuracy and reproducibility of subgrouping and grading soft-tissue sarcomas by fine-needle aspiration biopsy (FNAB), a blind review was conducted of 84 FNAB specimens from 77 malignant and 7 benign soft-tissue lesions. Cytomorphologic subgroups included 31 spindle-cell, 24 pleomorphic, 11 myxoid, 7 epithelioid/polygonal, 3 small round cell, and 8 nondiagnostic cases. Malignancies included one lymphoma and 41 primary, 15 recurrent, and 20 metastatic soft-tissue sarcomas. Adequacy was defined as a majority of slides with at least 5 clusters of 10 unobscured cells. Five originally false-negative cases were considered nondiagnostic on review. Sarcoma was recognized in 59 of 64 adequate cases (92%) with available histology; however, the specific histopathologic subtype was identified in only 9 cases (14%). Benign myxoid and spindle-cell lesions were difficult to separate from low-grade sarcomas in 4 cases, and a B-cell lymphoma with sclerosis mimicked a low-grade myxoid sarcoma. The assigned cytologic grade accurately reflected the histologic grade in 90% of sarcomas when segregated into high and low grades. Pleomorphic, small round cell, and epithelioid/polygonal subgroups corresponded to high-grade sarcomas in all cases with only minor noncorrelations. Major grading noncorrelations occurred in 50% of myxoid and 9% of spindle-cell sarcomas. Therefore, attention should be given to specimen adequacy, and caution should be exercised when attempting to grade myxoid and spindle-cell sarcomas by FNAB.     

聪灵胶囊对小鼠软脑膜微循环障碍的改善作用

目的 观察聪灵胶囊对小鼠软脑膜微循环障碍的改善作用。方法 将60只小鼠随机分为聪灵胶囊大、中、小剂量组、阳性对照组和正常对照组，分别于灌胃给药10d后，开颅窗观察小鼠软脑膜微循环，然后在软脑膜局部滴加去甲肾上腺素复制微循环障碍模型，观察微循环障碍时小鼠软脑膜微循环。结果 聪灵胶囊各剂量组均可改善去甲肾上腺素所致的小鼠软脑膜局部微循环障碍．使微动脉扩张，血流增快，每视野交织网点数增多，对血流流态也有一定的改善作用，使血色变红。9min后聪灵胶囊各剂量组软脑膜局部微循环障碍基本恢复，而空白对照组恢复不明显。结论 聪灵胶囊能改善小鼠软脑膜微循环。     

康妇炎胶囊联合米非司酮对输卵管异位妊娠患者疗效分析

目的 探讨康妇炎胶囊联合米非司酮治疗输卵管异位妊娠的临床效果和安全性。方法 选取2017年5月—2019年5月就诊于我院的84例输卵管异位妊娠患者,按随机数字表法分为两组,各42例。对照组予以米非司酮治疗,观察组加用康妇炎胶囊治疗。比较两组临床疗效、包块大小和血β-人绒毛膜促性腺激素(β-HCG)、性激素水平、不良反应情况。结果 观察组临床总有效率为92.86%,高于对照组的73.81%,差异有统计学意义(χ2=5.486,P=0.019);两组治疗后包块大小均较治疗前小,观察组治疗后包块大小为(0.84±0.12)cm,较对照组的(1.16±0.18)cm小(t=9.586,P＜0.001);两组β-HCG均较治疗前低,β-HCG为(312.04±63.65)U/L,较对照组的(435.14±68.41)U/L低,差异有统计学意义(t=8.538,P＜0.001);两组治疗后卵泡刺激素(FSH)、雌二醇(E2)和促黄体生成素(LH)水平低于治疗前,观察组治疗后FSH(12.03±2.11vs14.58±2.23,t=5.383,P＜0.001)、E2(143.69±15.62vs158.95±16.37,t=4.371,P＜0.001)、LH为(9.34±1.53vs13.05±2.18,t=9.028,P＜0.001)均低于对照组;两组不良反应相比,差异无统计学意义(χ2=0.138,P=0.457)。结论 康妇炎胶囊联合米非司酮可增强输卵管异位妊娠治疗效果,改善性激素水平,加快包块消失,促进β-HCG复常,安全可靠。     

蒙古族颅颌面软硬组织三维X线头影测量的临床意义

目的：研究中国蒙古族正常He成年人颅颌面软硬组织三维X线头影测量数据在临床中的作用。方法：选择符合正常He标准的蒙古族成年人98名（男女各半）,对颅颌面软、硬组织进行三维X线头影测量分析。结果：①颅颌面软组织的测量：蒙古族与汉族相比,面型较平直,面部较宽;与美国白人相比,蒙古族的上唇位于下唇前方,美国白人的上唇位于下唇后方。相对国人汉族而言,蒙古族上、下唇位置均靠后;蒙古族本民族的面型女性比男性宽而短。②颅颌面硬组织的测量：与蒙古族本民族相比,线距有性别差异,角度无性别差异,同一受检者左右相同项目测量,结果不完全一样。与不同民族相比,线距在矢状、横向、垂直三维方向与广东人、西安人有显著差异。结论：①蒙古族正常He成年人颅颌面左右侧的软硬组织存在着属于正常范围的不对称现象;②与不同民族和种族相比,蒙古族颅颌面软、硬组织形态具有明显的性别差异和种族特点;③本研究获取了蒙古族正常He成年人颅颌面软、硬组织的三维X线测量数据,建立了蒙古族自己的数据库;④蒙古族成年患者在正颌外科和牙齿正畸的诊断、设计和治疗中,应参考本民族颅颌面软硬组织的正常值和属于正常范围的面部左右不对称现象。     

乙肝清胶囊对实验性肝损伤的保护作用

目的：通过本实验观察乙肝清胶囊的保肝降酶作用。方法：实验采用四氯化碳（CCl4）和D－氨基半乳糖（D－Gal）,造成动物急性实验性肝损伤,测定小鼠和大鼠血清GPT和LDH,并进行组织形态学观察。结果：乙肝清胶囊灌胃给药对CCl4和D－Gal急性肝损伤所引起的小鼠和大鼠GPT和LDH升高,均具有明显的抑制作用,且对小鼠和大鼠肝组织病理损伤具有一定保护作用,其1－2g/kg与联苯双酯15mg/kg作用效果接近。结论：乙肝清胶囊对化学物质的所引起的小鼠和大鼠急性肝损伤,具有保肝降酶作用。