THE EFFECTS OF MA-XING-GAN-SHI-TANG ON RESPIRATORY RESISTANCE AND AIRWAY LEUKOCYTE INFILTRATION IN ASTHMATIC GUINEA PIGS

Ma-Xing-Gan-Shi-Tang (MXGST), a traditional Chinese medicine, has been used in treatment of the bronchial asthma for several centuries. However, the therapeutic mechanisms of this Chinese medicine are still far from clear. To understand the mechanism of anti-asthmatic property of MXGST, a guinea pig model of allergic asthma was used to investigate the effects of MXGST on Dermatophagoides pteronyssinus-induced early and late asthmatic responses and airway inflammation, and examine direct β₂-adrenoceptor agonist activity in guinea-pig isolated trachea. Administration of MXGST (10 g/kg) extracts significantly inhibited the antigen induced immediate asthmatic responses (IAR) in actively sensitized guinea pig. MXGST caused concentration-dependent relaxation in strips of guinea pig trachea contracted with carbachol, and ICI-118551, a selective β₂-adrenoceptor antagonist, significantly inhibit the relaxation caused by MXGST. Furthermore, examination of bronchoalveolar lavage fluid (BALF) revealed that MXGST significantly inhibited the increase in neutrophil in the airway at 1, 6 and 24 hr after antigen challenge. Histopathologic examination results showed that MXGST suppressed the neutrophil infiltration into lung tissue. In conclusion, we suggest that the anti-asthmatic effects of MXGST are mainly due to its stimulation of β₂-adrenoceptors on bronchial smooth muscle and its anti-inflammatory ability to inhibit the neutrophil into the airway. The precise mechanism of action of MXGST in asthma remains to be elucidated.     

The Effect of Jeo Dang‐Tang on Cytokines Production in the Patients with Cerebral Infarction

Abstract

The herbal formulation “Jeo Dang‐Tang” (JDT) has long been used for various cerebrovascular diseases. However, very little has scientific investigation been carried out. The aim of the present study is to investigate the effect of JDT on the production of various cytokines in the patients with cerebral infarction (CI). Peripheral blood mononuclear cells (PBMC) obtained from the patients with CI were cultured for 24 h in the presence or absence of lipopolysaccharide (LPS) or phytohemagglutinin (PHA). The amount of interleukin (IL)‐4, IL‐10 and transforming growth factor (TGF)‐1β, in culture supernatant, was significantly increased in the JDT, LPS or PHA treated cells compared to unstimulated cells (P < 0.05). We also show that increased IL‐4, and IL‐10 level by LPS or PHA was significantly inhibited by JDT in a dose‐dependent manner. Maximal inhibition rate of IL‐4 and IL‐10 production by JDT was 45 ± 2% and 51 ± 5% for LPS‐stimulated cell and 41.5 ± 3% and 70.8 ± 2% for PHA‐stimulated cells, respectively (P < 0.05). On the other hand, JDT significantly increased the LPS or PHA‐induced TGF‐β1 production (P < 0.05). These data suggest that JDT has a regulatory effect on the cytokines production, which might explain its beneficial effect in the treatment of CI.     

Effect of the herbal medicine Danggui Sini plus Wuzhuyu Shengjiang Tang on erythrocyte deformability in normal subjects: a cross-over trial

OBJECTIVE

To show whether Danggui Sini plus Wuzhuyu Shengjiang Tang (DSWST) has any transient effect on erythrocyte deformability in normal subjects.

从开阖枢理论探讨血府逐瘀汤临床验案4则

血府逐瘀汤是王清任《医林改错》中经典方剂,一般认为,本方为活血祛瘀、行气止痛之剂,主治胸中血府血瘀。从"开阖枢"理论理解此方,验之临床,疗效独特。     

血府逐瘀汤对肺心病患者的疗效及血液流变学影响简

目的 探讨血府逐瘀汤对肺心病患者的临床疗效以及对血液流变学的影响.方法 将76例患者随机分为对照组和治疗组,各38例,对照组给予西医基础治疗,治疗组在对照组基础上每日给予1剂血府逐瘀汤,分早晚2次温服,2周为1个疗程.统计数据以3个疗程为准.结果 对照组总有效率为65.79％,治疗组总有效率为89.47％,差异有统计学意义（P＜0.05）;治疗组全血高切黏度、全血低切黏度、纤维蛋白原、血浆黏度,肺功能（最大通气量、时间肺活量、残气/肺总量）以及心功能（每搏输出量、左心室射血分数、心输出量）与对照组比较,差异有统计学意义（P＜0.05）.结论 血府逐瘀汤有效改善高黏血症,对肺原性心脏病有显著疗效,具有重要临床意义,值得研究推广.     

王丽平教授应用苓桂术甘汤为主配合腹针临证经验

目的总结王丽平教授临床应用苓桂术甘汤配合腹针临证经验。方法通过苓桂术甘汤配合腹针治疗眶肌炎、结节性痒疹及干燥综合征的医案来具体阐述王丽平教授应用苓桂术甘汤及腹针治疗思路。结果苓桂术甘汤配合腹针治疗眶肌炎、结节性痒疹及干燥综合征疗效显著。结论苓桂术甘汤加减应用配合腹针治疗体现了王丽平教授"脾胃为基""三焦立论"的学术思想。     

加味芍药甘草汤配合中药熏洗治疗痉挛性肛门直肠痛疗效观察

目的探讨加味芍药甘草汤配合中药熏洗治疗痉挛性肛门直肠痛的临床效果。方法所有病例采用加味芍药甘草汤煎汤治疗,观察并记录治疗前及治疗后4周的肛门疼痛情况。结果 52例病例治疗后总有效率为90.4%,治疗前后疼痛评分积分比较差异有统计学意义。结论加味芍药甘草汤配合中药熏洗治疗痉挛性肛门直肠痛具有恢复快、疗效佳的特点。     

新血府逐瘀软胶囊对动脉粥样硬化血瘀证患者MMP-9和TIMP-1的影响

目的研究新血府逐瘀软胶囊对动脉粥样硬化患者基质金属蛋白酶-9(matrix metalloproteinase 9/MMP-9)及金属蛋白酶组织抑制剂-1(tissue inhibitor of metal protease 1/TIMP-1)的影响,并探讨其可能的作用机制。方法将60例动脉粥样硬化血瘀证患者随机分为对照组(n=30)和治疗组(n=30),对照组予阿托伐他汀钙片,治疗组予阿托伐他汀钙片和新血府逐瘀软胶囊,观察12周。对比两组治疗前后血清MMP-9、TIMP-1水平和血瘀证积分。结果两组自身前后比较,两组MMP-9及血瘀证积分水平均较本组治疗前明显下降(P0.05或P0.01);TIMP-1水平升高(P0.05或P0.01)。治疗结束后组间比较,治疗组MMP-9及血瘀证积分水平均明显低于对照组(P0.05);TIMP-1水平明显高于对照组(P0.05)。结论新血府逐瘀软胶囊能够降低血清MMP-9的水平,增高TIMP-1水平,降低血瘀证积分,改善动脉粥样硬化患者血瘀征象,具有抗动脉粥样硬化的作用,提示其可能通过降低炎症反应从而抑制动脉粥样硬化。     

中药糖通饮方治疗早期糖尿病肾病患者临床观察

[目的]通过观察中药糖通饮方对早期糖尿病肾病(DN)患者各指标的影响,评估其临床药物安全可靠性,系统量化评价其临床疗效,初步探讨其可能的作用机制,为防治DN提供一种安全、经济、有效的中药处方。[方法]将100例早期DN患者按随机数字表法随机分为对照组50例、治疗组50例,分别予以相应的方法治疗3个月,同时检测治疗前后患者各指标。[结果]两组治疗前后的血常规(BRT)、大便常规(SRT)、小便常规(URT)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、总胆红素(TBIL)、间接胆红素(IBIL)、直接胆红素(DBIL)、肌酐(Scr)、血清尿素氮(BUN)和γ-谷氨酰转肽酶(GGT)无变化(P0.05)。两组治疗前后的血浆血管内皮生长因子(VEGF)、尿微量白蛋白/肌酐(Alb/Cr)、糖化血红蛋白(HbA1c)、空腹血糖(FBG)、餐后2 h血糖(2HBG)、血清胰岛素(Insulin)均有不同程度的下降,内生肌酐清除率(Ccr)升高,差异有统计学意义(P0.05或P0.01)。治疗组较对照组VEGF、Alb/Cr、FBG、2HBG、HbA1c下降,Ccr升高,差异有统计学意义(P0.05)。[结论]中药糖通饮方临床药物安全可靠,其对早期DN患者的肾脏病变有一定的保护作用,值得临床推广。     

不同煎煮方法对血府逐瘀汤浸出物和主成分的影响及多科室普及应用研究

目的:观察和探讨不同煎煮方法对血府逐瘀汤浸出物和主成分的影响,针对固方汤剂多科室普及应用,扩大医院制剂发展提供新的思路和方法。方法:入选于本院就诊的冠心病心绞痛患者70例,下肢静脉血栓40例,慢性肾功能不全30例,将入选患者随机分为观察组和对照组,采用双盲原则进行试验研究,治疗组给予固方血府逐瘀汤治疗,对照组给予阳性对照药物干预,对两组患者治疗前后疗效性进行对比研究。结果:传统煎煮对比煎药机煎煮总浸出物含量明显较高,差异显著,P0.05;煎药机煎煮羟基红花黄色素A、芍药苷和柚皮苷含量均较传统煎煮有所提高,差异显著,P0.05;在多科室应用血府逐瘀汤进行异病同治原则下的辨证治疗,研究结果显示,冠心病心绞痛总有效率高达92.1%,下肢静脉血栓总有效率高达93.2%,慢性肾功能不全总有效率高达89.7%,组间差异均具有显著性,P0.05。结论:煎药机煎煮法浸出物含量较高,具有一定优势,将血府逐瘀汤进行多科室普及应用具有积极意义。