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51.
Vladimir Kenis Eugeniy Melchenko Ilya Mazunin Minna Pekkinen Outi Mäkitie 《American journal of medical genetics. Part A》2021,185(1):112-118
Epiphyseal chondrodysplasia, Miura type (ECDM) is a skeletal dysplasia with tall stature and distinctive skeletal features caused by heterozygous NPR2 pathogenic variants. Only four families have been reported. We present a family with five affected individuals (mother, three sons, and daughter). The mother's phenotype was relatively mild: borderline tall stature and elongated halluces operated during childhood. The children were remarkably more severely affected with tall stature, scoliosis, and elongated toes and fingers leading to suspicion of Marfan syndrome. Progressive valgus deformities (at the hips, knees, and ankles) were the main complaints and necessitated orthopedic investigations and surgery. Radiographs showed coxa valga, scoliosis, multiple pseudoepiphyses of the fingers and toes with uneven elongation of the digits and ankle valgus. The two older brothers underwent osteotomies and guided growth for axial deformities and arthrodesis for elongated halluces. Genetic testing confirmed the clinical diagnosis of ECDM: all affected individuals had a heterozygous c.2647G>A (p.Val883Met) NPR2 variant in a highly conserved region in the carboxyl‐terminal guanylyl cyclase domain. This two‐generation family elucidates the clinical and radiological variability of the disease. These rare cases are important to gain further understanding of the fundamental processes of growth regulation. 相似文献
52.
Rutger L. van Bezooijen Marco C. DeRuiter Nathalie Vilain Rui M. Monteiro Annemieke Visser Lianne van der Wee‐Pals Conny J. van Munsteren Pancras C.W. Hogendoorn Michel Aguet Christine L. Mummery Socrates E. Papapoulos Peter Ten Dijke Clemens W.G.M. Löwik 《Developmental dynamics》2007,236(2):606-612
Spatial-temporal regulation of bone morphogenetic protein (BMP) and Wnt activity is essential for normal cardiovascular development, and altered activity of these growth factors causes maldevelopment of the cardiac outflow tract and great arteries. In the present study, we show that SOST, a Dan family member reported to antagonize BMP and Wnt activity, is expressed within the medial vessel wall of the great arteries containing smooth muscle cells. The ascending aorta, aortic arch, brachiocephalic artery, common carotids, and pulmonary trunk were all associated with SOST expressing smooth muscle cells, while the heart itself, including the valves, and more distal arteries, that is, pulmonary arteries, subclavian arteries, and descending aorta, were negative. SOST was expressed from embryonic day 15.5 up to the neonatal period. SOST expression, however, did not correspond with inhibition of Smad-dependent BMP activity or beta-catenin-dependent Wnt activity in the great arteries. Activity of both signaling pathways was already down-regulated before induction of SOST expression. 相似文献
53.
L. S. Jen R. D. Lund J. Boles 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1978,33(3-4):405-414
Summary An aberrant crossed corticotectal pathway can be generated by removal of one visual cortex and the contralateral superior colliculus from newborn rats. This aberrant crossed corticotectal projection arises from the pyramidal neurons located in layer V of the visual cortex and terminates in a spatially orderly manner in the appropriate laminae of the cortically deafferented contralateral colliculus. Comparable results cannot be reproduced by unilateral collicular lesions alone. The significance of these findings and the possible mechanisms involved in the formation of the aberrant pathway are discussed and compared with the retinotectal system.The research was supported by USPHS Grant EY-00596 from the National Institutes of Health 相似文献
54.
目的:从基因水平研究肝移植急性排斥反应中T淋巴细胞信号传导途径。方法:利用4096条大鼠cDNA克隆的基因芯片对从急性排斥组Wistar→SD(n=5)和对照组SD→SD(n=5)两组肝移植大鼠T细胞中抽提、纯化mRNA,扩增、逆转录成cDNA,荧光标记后与芯片杂交,扫描后筛选出差异表达的基因。结果:在4096个基因中共发现差异表达基因190条,其中ll条与细胞信号传导有关的基因差异表达明显:MHC(3条)、CD3抗原(1条)相关基因;蛋白酶类:蛋白激酶C结合蛋白、蛋白酪氨酸磷酸酯酶D型受体、磷脂酰肌醇二磷酸酶基因各l条;涉及核酸信号传导、激活、转录、翻译的基因3条。结论:T细胞在肝移植术后排斥反应中信号传导机制涉及多个基因,基因芯片技术的大规模筛选为进一步研究T细胞在排斥反应中的信号传导途径提供了客观依据和目标。 相似文献
55.
Dissecting natural killer cell activation pathways through analysis of genetic mutations in human and mouse 总被引:2,自引:1,他引:2
Summary: Natural killer (NK) cell cytotoxicity is mediated by multiple germ line-encoded activating receptors that recognize specific ligands expressed by tumor cells and virally infected cells. These activating receptors are opposed by NK inhibitory receptors, which recognize major histocompatibility complex class I molecules on potential targets, raising the threshold for NK cell activation. Once an abnormal cell has been detected, NK cells are the sentinel source of cytolytic mediators, such as granzymes and perforins, as well as interferon-γ, which can polarize the immune response to a T-helper 1 cell type. Activation signals are transmitted by adhesion-dependent pathways, immunoreceptor tyrosine-based activation motif (ITAM)-dependent pathways, DAP10 ITAM-independent pathways, and by signaling through immunoreceptor tyrosine-based switch motifs. These pathways activate downstream signaling partners to trigger NK cell cytotoxicity. Some of these downstream molecules are unique to the various pathways, and some of these molecules are shared. Because of the complexity of signals involved in NK cell–target cell interaction, the generation of mice with targeted mutations in signaling molecules involved in adhesion, activation, or inhibition is essential for a precise dissection of the mechanisms regulating NK cell effector functions. Here we review recent advances in the genetic analysis of the signaling pathways that mediate NK cell killing. 相似文献
56.
目的 进一步研究NOD小鼠T细胞应答改变机理。方法 用抗TCR抗体、ConA激活NOD小鼠胸腺细胞,分析TCR介导的信号通路的水平。结果 与Balb/c小鼠胸腺细胞相比,抗TCR抗体诱导的增殖应答较弱,与年龄及NOD胸腺CD4^ CD8^-和CD4^-CD8^ SP细胞有关;rIL-2能部分恢复对TCR抗体应答的缺乏。NOD小鼠对PMA IONO和PMA anti—TCR-mAb应答正常,但对anti-TCRmAb IONO应答缺乏。结论 与年龄有关的NOD小鼠胸腺细胞对TCR抗体应答的缺乏与T细胞激活时上游PKC信号通路的缺乏有关。 相似文献
57.
58.
Antibody content against rabbit red blood cells (anti-RaRBC) in murine sera of different strains (Swiss, CBA, C57BL/6, AKR, BALB/c) and activity of complement alternative pathway (AP) were investigated. In contrast to the CBA and C57BL/6, random-bred Swiss strain and inbred BALB/c and AKR strains are good producers of these natural antibodies. There is no correlation between AP activity and anti-RaRBC content. Isolated human anti-RaRBC antibodies, IgM and IgG classes, lead to the enhancement of APhu and APmo activity, contrary to the murine anti-RaRBC which belong solely to IgM class, and do not express this capability. 相似文献
59.
L. D. Luk'yanova G. N. Chernobaeva V. E. Romanova 《Bulletin of experimental biology and medicine》1989,107(4):482-484
Institute of Pharmacology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. V. Val'dman.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 107, No. 4, pp. 431–433, April, 1989. 相似文献
60.
浸润和转移是恶性肿瘤的重要特征,也给肿瘤的治疗带来困难,是预后不良的重要因素.转移抑制因子23(non-metastasis,NM23)基因是最早发现的抗肿瘤转移基因之一.现在已经发现NM23是一个基因家族,包括NM23-H1、NM23-H2等重要的基因家族成员.研究表明NM23基因表达与实体瘤转移抑制有关,在很多实体瘤中可以作为进展和预后的分子标记.随着对NM23基因调控肿瘤转移的分子机制的研究的进一步开展,已经发现了一些NM23肿瘤转移抑制通路上下游的相关调控分子,为进一步的信号通路研究创造了条件.本文概述了近年来对NM23基因转移抑制通路研究的新近展,提出了以后可能的研究方向和需要解决的关键问题. 相似文献