一些稀土元素与西佛碱水杨叉邻氨基苯酚配合物的研究

作者合成了西佛碱水杨叉邻氧基苯酚和某些稀土元素固态配合物,这些配合物的组成分别为 RE.HL.L(RE=Ce Pr Nd)和 R_(B2)L_3,(RE=La Sm Eu Gd Lu),根据元素分析,电导、红外光谱、核磁、电子光谱和荧光光谱对配合物进行了表征。     

Novel Detection Method for Circulating EGFR Tumor DNA Using Gravitationally Condensed Gold Nanoparticles and Catalytic Walker DNA

The detection of circulating tumor DNA is a major challenge in liquid biopsies for cancer. Conventionally, quantitative polymerase chain reactions or next-generation sequencing are used to detect circulating tumor DNA; however, these techniques require significant expertise, and are expensive. Owing to the increasing demand for a simple diagnostic method and constant monitoring of cancer, a cost-effective detection technique that can be conducted by non-experts is required. The aim of this study was to detect the circulating tumor DNA containing the epidermal growth factor receptor (EGFR) exon 19 deletion, which frequently occurs in lung cancer. By applying walker DNA to a catalytic hairpin assembly and using the differential dispersibility of gold nanoparticles, we detected EGFR exon 19 deletion mutant #2 DNA associated with lung cancer. Our sensing platform exhibited a limit of detection of 38.5 aM and a selectivity of 0.1% for EGFR exon 19 wild-type DNA. Moreover, we tested and compared EGFR exon 19 deletion mutants #1 and #3 to evaluate the effect of base pair mismatches on the performance of the said technique.     

不同种类黄芪中多糖含量的比较

目的：本文对不同品种(蒙古黄芪、膜英黄芪)黄芪中多糖成分进行含量测定。方法：运用水提醇沉法提取蒙古黄芪,膜荚黄芪,用苯酚-浓硫酸比色法测定多糖含量。结果：以葡萄糖为对照品,苯酚-浓硫酸比色法测定线性关系良好,平均回收率99．50%,RSD1．25%。结论：不同品种,不同产地黄芪根中含有的多糖存在较大差异。     

紫外/可见分光光度法测定地呋麻滴鼻液中呋喃西林和盐酸麻黄碱的含量

目的建立地呋麻滴鼻液中呋喃西林和盐酸麻黄碱的含量测定方法,为制剂的质量控制提供有效分析方法。方法采用紫外-可见分光光度法测定地呋麻滴鼻液中呋喃西林和盐酸麻黄碱的含量。结果呋喃西林和盐酸麻黄碱在紫外-可见光区内有最大吸收,辅料等其他成分对两主药无干扰。本方法用于测定呋喃西林和盐酸麻黄碱的含量,回收率分别是99.5%、99.4%,RSD小于1.0%。结论方法专属性强,操作方便,结果准确,重现性好。     

苗药金铁锁药材和植物细胞培养产生的总皂苷含量比较

目的 比较金铁锁药材和植物细胞培养产生的植物愈伤组织、毛状根中总皂苷含量.方法 采用紫外可见分光光度法,以齐墩果酸为对照品,在波长535 nm处测定样品中的总皂苷含量.结果 齐墩果酸在0.008 112～0.048 672 mg/ml范围内呈现良好的线形关系,该法平均加样回收率为100.77%,RSD=2.44%(n= 9).测得金铁锁药材,金铁锁植物愈伤组织1,金铁锁植物愈伤组织2和金铁锁毛状根中总皂苷的含量分别为0.217%,0.388%,0.221%和0.857%.结论 金铁锁悬浮培养毛状根中总皂苷含量较高,应用价值较大.     

地氯雷他定含量两种测定方法的比较

目的对地氯雷他定的含量测定两种方法进行比较。方法通过容量法(非水滴定法)及紫外分光光度法对地氯雷他定作含量方法比较。结果紫外分光光度法线性范围4.89~29.34 mg.L-1,r=0.999 99,精密度RSD为0.73%;非水滴定法精密度RSD为0.11%。结论容量法检测地氯雷他定含量操作简单,方法快速准确。     

槲皮素与黄嘌呤氧化酶相互作用的紫外光谱性质研究

目的：研究了槲皮素与黄嘌呤氧化酶相互作用的紫外光谱性质及孵育时间对酶抑制性的影响。方法：紫外光谱法。结果：槲皮素与黄嘌呤氧化酶相互作用导致了槲皮素的紫外光谱的Ⅱ带红移。吸收峰升高;I带兰移,吸收峰下降。另外,随着两者孵育时间的增加,紫外光谱吸收峰下降。结论：槲皮素对黄嘌呤氧化酶有抑制性．并随着抑制时间的增加而增加。     

Controlled activity polymers. VIII. Copolymers of acrylic acid and isomeric N-akylacrylamide with pendent β-naphthol esters moieties: Synthesis and characterization

Monomers possessing hydrolytically-labile bonds to the bioactive agent β-naphthol (βN) were prepared for incorporation into hydrophilic copolymers. β-Naphthyl acrylate was synthesized by an esterification reaction of β-naphthol with acryloyl chloride. 5-Acrylamido(β-naphthyl)valerate, 2-acrylamido(β-naphthyl)isovalerate, 3-acrylamido-3-methyl(β-naphthyl)butanoate, and 6-acrylamido(β-naphthyl)caproate were synthesized by esterification of βN with 5-acrylamidovaleric acid, 2-acrylamidovaleric acid, 3-acrylamido-3-methylbutanoic acid, and 6-acrylamidocaproic acid, respectively, utilizing 1,3-dicyclohexylcarbodiimide as a coupling agent and 4-dimethylaminopyridine as a catalyst. The βN monomers were free radically polymerized with acrylic acid, utilizing AIBN as initiator in benzene at 70°C to obtain polymer-bound allelopathic βN. Copolymer composition was determined by ¹H-NMR, ¹³C-NMR, FTIR, and UV-vis. spectroscopy. The copolymerization reactivity ratios were calculated from ¹H-NMR data using Fineman-Ross, Kelen-Tudos (K-T), and nonlinear least-squares methods. Copolymer microstructural predictions were determined from calculated K-T reactivity ratios. The resulting water soluble copolymers prepared in this work are potentially useful as plant growth regulators through the hydrolytic release of β-naphthol. These model structures have been utilized for the determination of structure/controlled release relationships reported in an accompanying publication.