肝细胞癌SCT表现特征与Rb,PCNA的关系

目的　探讨肝细胞癌 (HCC)SCT (spiralCT)表现特征与癌细胞中视网膜母细胞瘤基因(Rb)、增殖细胞核抗原 (PCNA)表达的关系。方法　对 39例 (41个病灶 )经手术病理证实且行SCT动、静脉双期增强扫描的肝细胞癌病例 ,观察每个病灶的SCT表现特征 :肿瘤的大小、包膜、侵袭危险性、强化类型和肝硬化情况。用免疫组化法检测癌组织中Rb ,PCNA表达 ,分析评价SCT表现特征与蛋白之间的关系。结果　HCCSCT图像中肿瘤大小、包膜形成、侵袭危险性、强化类型和周围肝组织有无硬化与Rb蛋白之间无统计学意义 (P >0 .0 5 )。肿瘤大小、侵袭危险性、强化类型与PCNA之间有统计学意义 (P <0 .0 5 )。结论　肝细胞癌的SCT表现特征与PCNA的表达相关 ,根据其表现特征 ,可在一定程度上推测肿瘤组织PCNA表达情况 ,这有助于HCC的早期诊断及治疗方案的选择     

卫氏并殖吸虫成虫的特异性诊断抗原

目的：寻找诊断并殖吸虫病的特异性抗原。方法：应用SDS-PAGE和Western blot对卫氏并殖吸虫成虫可溶性抗原中的蛋白组分进行分析。结果：卫氏并殖吸虫成虫可溶性抗原蛋白经SDS-PAGE后显示14条蛋白带，其中相对分子质量为53000、3l000、25000、16000、11000是主带；相对分子质量为53000和34000的条带可与华支睾吸虫病患者血清反应；108000、94000、65000的条带可与血吸虫病患者血清反应；58000、53000、43000的条带可与旋毛虫感染的大鼠血清、小鼠血清及旋毛虫病患者血清反应。37000的蛋白带只能被斯氏狸殖吸虫感染的大鼠血清和并殖吸虫病患者血清所识别，而不与华支睾吸虫病、血吸虫病患者血清、旋毛虫感染的大鼠和小鼠血清、旋毛虫病患者血清、正常大鼠、小鼠血清及正常人血清发生交叉反应。结论：卫氏并殖吸虫成虫可溶性抗原中相对分子质量为37000的蛋白组分为卫氏并殖吸虫成虫的特异性抗原，可用于并殖吸虫病的免疫学诊断及血清流行病学调查．     

Familial spontaneous pneumothorax in three generations and its HLA

We experienced a case of familial spontaneous pneumothorax in three generations. Six of 13 family members had episodes of spontaneous pneumothorax. It is well established that there are some diseases associated with human leukocyte antigen (HLA). We performed HLA phenotyping for HLA of A, B and C. In our study, we detected the HLA haplotype A2, B61 in three of 4 who had episodes of spontaneous pneumothorax. The HLA haplotype A2, B70 were also detected in three of 4 who had episodes. This suggests that familial spontaneous pneumothorax might have hereditary factors.     

男科学组织工程研究前景

利用组织工程技术进行细胞移植的设想 ,已经为男科领域的组织再建工作提供了许多可能。为改善、修复或替代现有组织的功能 ,组织工程的应用研究 ,已经在睾丸间质细胞、睾丸假体、阴茎海绵体、阴茎假体等方面开展。虽然大多数再建工作仍然停留在实验阶段 ,但是有些技术也被用于临床 ,并取得满意的结果。本文简要地综述了组织工程在男科学的应用     

应用结合于内皮细胞的尿激酶预防血栓形成

提纯出受损伤内皮细胞的特异性抗原，再用免疫学方法制得相应抗体，并把它与尿激酶形成结合物。在人工造成血管内皮细胞损伤的动物中，分别用尿激酶结合物、单纯特异性抗体处理，各组动物处死后进行形态学观察。结果显示：在未用尿激酶结合物处理的动物血管内和使用结合物处理的血管内，血栓形成的程度有明显的差别，前者明显，后者轻微。这种方法既能预防血栓形成，又不会产生继发性出血的危险。     

衰老红细胞与带3蛋白

循环系统里的成熟红细胞在其生命过程里经历着氧化性衰老，相应细胞组份出现血红蛋白变性、膜蛋白交联、带3蛋白聚集以及带3蛋白降解等多种修饰。这些修饰作为红细胞膜上的衰老信号参与构成衰老细胞抗原，由此启动与IgG自身抗体(主要为带3蛋白抗体)的结合以及补体的沉淀，最终是免疫吞噬系统对异常细胞的识别清除．现就衰老红细胞与带3蛋白的关系作一综述。     

增殖细胞核抗原在鼻咽癌组织中的表达及其临床意义

目的：探讨鼻咽癌（ＮＰＣ）组织ＰＣＮＡ的表达与其临床进展、病理组织分裂的相互关系。方法：用免疫组化Ｓ－Ｐ法检测１１３例ＮＰＣ和２５例鼻咽慢性炎的ＰＣＮＡ表达。结果：１０３例ＮＰＣ和３例鼻咽慢性炎呈ＰＣＮＡ阳性。ＮＰＣ呈ＰＣＮＡ低增殖频率者，倾向于Ｉ、Ⅱ期临床分期（８７．１％）及无淋巴结转移（７０．９６％）。高增殖频率者，倾向于Ⅲ、Ⅳ期临床分期（８２．１％）及有淋巴结转移（７９．４９％）。增殖频率与     

The use of tissue expanders in syndactyly repair

The syndactyly repairs of 11 hands in seven patients (average age 20.4) who had simple complete syndactyly between the third and fourth fingers were done by the use of tissue expanders in order to obtain adequate skin closure. At the first stage, a wedge type, 7 cc tissue expander was placed beneath the dorsal skin of the syndactylous digits via a vertical dorsal hand incision under local anesthesia. Inflation was begun on the 15th postoperative day. After the inflation period (average 32 days), syndactyly repair similar to the Littler technique was performed using axillary block anesthesia. During the expansion period one tissue expander became exposed and this hand was repaired by the classical skin grafting technique. In the remaining ten hands the expanded dorsal skin ensured skin closure of the digits and web reconstruction without any need for skin grafting. No complications were encountered during the postoperative period. Active and passive abduction angles, tip-to-tip length, active range of motion of the digits, and two point discrimination tests were done at follow-up (average six months). The web appearances were normal in all patients. Active abduction angles and active range of motion of the digits was always normal at the third postoperative month. Two point discrimination was 8.7 mm on the interdigital sides of the digits and there was no tip-to-tip length differences of the digits. No contractures were seen.Presented at the International Congress of Surgery of the Hand and Upper Extremity, Izmir, Turkey, September 25, 1994.     

Pemphigoid Nodularis: A Case with 230 kDa Hemidesmosomes Antigen Associated with Bullous Pemphigoid Antigen

We report a 73-year-old woman with typical clinical, histological and immunofluorescence features of pemphigoid nodularis. Direct immunofluorescence studies of prurigo nodularis-like lesions and peribullous skin showed the linear deposition of IgG and C3 at the basement membrane zone. Circulating IgG against the basement membrane was also detected by indirect immunofluorescence. The serum from the patient was shown to contain the autoantibody against 230 kDa hemidesmosomal antigen associated with bullous pemphigoid antigen.