安宫牛黄丸的实验药理学研究

目的:探讨安宫牛黄丸的实验研究内涵。方法:根据安宫牛黄丸实验研究资料分类介绍。结果:安宫牛黄丸实验研究内涵主要体现在:对方中黄芩、栀子、郁金、黄连、牛黄、麝香、朱砂、雄黄做了质量控制研究;对方中朱砂、雄黄是否必需及其药代动力学状况进行了初步研究;该药通过对急性脑损伤动物模型脑组织、脑电波、单胺类和氨基酸类神经递质、一氧化氮、氧化损伤与抗损伤因子、乳酸脱氢酶及其同工酶、肌酸激酶的调节,产生清热解毒、镇惊开窍的效用;实验还发现该药有解热、镇静、抗缺氧等作用,未发现抗惊厥作用;可试用于白血病的治疗;对肺、肝有保护作用;生理状态下较长时间服用对心肌、肾、红细胞、脾等有一定的损伤,病理状态下损伤较小。结论:这些研究成果对进一步提升该药的质量标准、推广临床应用具有重要意义。     

左归丸对大鼠视神经损伤后相关基因表达的影响

目的:观察大鼠视神经夹伤后视网膜中GAP-43和Nogo-A蛋白的动态变化,探讨左归丸对大鼠视神经部分损伤后再生修复的影响。方法:建立大鼠单侧视神经部分损伤模型,分别给予左归丸液(实验组,2.0g.kg-1.d-1)和等量生理盐水(对照组)灌胃,采用免疫组织化学技术于伤后不同时间点检测GAP-43及Nogo-A蛋白在受损眼视网膜中的表达。结果:视神经损伤后,GAP-43和Nogo-A蛋白表达先升高后下降,伤后2～4周时左归丸组GAP-43表达显著上调,而Nogo-A表达基本降至正常水平,与对照组相比,差异有显著性(P<0.01)。结论:左归丸可显著促进GAP-43的表达,同时抑制Nogo-A表达,促进受损视神经再生修复。     

左金丸合香砂平胃散治疗胆汁反流性胃炎120例

目的：观察左金丸合香砂平胃散治疗胆汁反流性胃炎的疗效。方法：120例用左金丸合香砂平胃散治疗40天。结果：治愈68例,显效22例,有效20例,无效10例,总有效率91．7％。结论：用左金丸合香砂平胃散治疗胆汁反流性胃炎疗效较好。     

肉蔻五味丸对大鼠心肌梗死后左室重构的影响

目的探讨肉蔻五味丸对心肌梗死后左室重构大鼠心肌基质金属蛋白酶（MMPs）的影响。方法 SD大鼠36只,随机分为正常对照组、心肌梗死模型组、肉蔻五味丸治疗组。模型组和治疗组大鼠腹腔注射异丙基肾上腺素（IS0）,治疗组给予肉蔻五味丸1g/kg·d灌胃,至各相应时段。称重并计算左室重量指数,免疫组化SABC法测各组不同时段心肌间质MMP-2、MMP-9表达,光镜下观察心肌病理改变。结果模型组大鼠左室重量指数高于正常对照组,心肌间质MMP-2、MMP-9表达在各时段明显高于对照组。肉蔻五味丸组左室重量指数、MMPs活性均显著低于模型组（P〈0.05或P〈0.01）。结论大鼠心肌梗死后心肌间质内的MMP-2、MMP-9表达升高,是心室重构的重要原因之一。蒙药肉蔻五味丸对MMPs活性的抑制可能是其干预治疗心肌梗死后左室重构的机制之一。     

HPLC法测定脑血栓丸(Ⅰ号)中芍药苷的含量

[目的]建立HPLC法测定脑血栓丸(Ⅰ号)中芍药苷的含量.[方法]以Inertex C18(5 μm,250 mm×4.6mm)为色谱柱;乙腈-水(15:85)为流动相;流速:1.0 mL/min;检测波长:230nm.[结果]芍药苷线性关系范围0.608～3.04μg,相关系数r=0.999 9,回收率102.7%,RSD=2.0%.[结论]此方法准确、可靠、重现性好,可适用于脑血栓丸(Ⅰ号)中芍药苷含量的测定.     

Systematic review and Meta-analysis of 26 randomized controlled clinical trials of Compound Danshen Dripping Pill for non-proliferating diabetic retinopathy

Objective: Diabetic retinopathy (DR) is the retinal consequence of chronic progressive diabetic microvascular leakage and occlusion. Non-proliferating diabetic retinopathy (NPDR) is the early stage of DR. It eventually occurs to some degree in all patients with diabetes mellitus. In recent years, many clinical trials have shown that Compound Danshen Dripping Pill (CDDP) may be associated with the improvement of NPDR symptoms. The aim of this study was to quantitatively summarize the association between CDDP and the therapeutic effects of NPDR. Methods: It was conducted that a systematic literature search of PubMed, Web of Science, CNKI, VIP and Wanfang Data updated in June 2020 with the following search terms: “diabetic retinopathy” or “retinopathy” or “DR” or “NPDR”, in combination with “Compound Danshen Dripping Pill” or “Salvia miltiorrhiza” or “Danshen”. Risk ratio (RR) and weighted mean difference (WMD) with their 95% confidence interval (CI) was calculated between treatment and control groups. The sensitivity analyses were undertaken by removing each individual study when high heterogeneity appeared. Subgroup analysis, Meta-regression, and publication bias analysis were also conducted. The strength of evidence was evaluated with the Grading of Recommendation, Assessment, Development, and Evaluation (GRADE) method. Results: Twenty-six RCTs involving 2047 subjects were included to conduct a Meta-analysis after screening the studies, extracting the data, and assessing the study quality. The Stata15.0 software was utilized for processing. Meta-analysis indicated that curative effects of treatment group with CDDP was significantly better than control (RR=0.54, 95% CI (0.40, 0.73); moderate-quality evidence). In addition, the results showed that CDDP was significantly associated with improving retinal hemorrhages (WMD=?0.62, 95% CI (?0.78, ?0.46); low-quality evidence), the vision (WMD=0.14, and 95% CI (0.09, 0.19), low-quality evidence), fundus fluorescence angiography (RR=0.37 and 95% CI (0.23, 0.60); low-quality evidence), reduction of retinal microaneurysm (WMD=-3.74 and 95% CI (?4.38, ?3.11); moderate-quality evidence), hemangioma volume (WMD=?3.15, 95%CI (?3.45, ?2.85); moderate-quality evidence), macular thickness (WMD=?5.52, 95%CI=(?64.27, ?48.78); low-quality evidence), mean defect (WMD=?1.65 and 95% CI (?1.95, ?1.34); very low-quality evidence), fasting blooding glucose (WMD=?0.95, 95% CI (?1.19, ?0.70); low-quality evidence), hemoglobin A1c (WMD=?0.62, 95% CI (?0.93, ?0.30); low-quality evidence), high sensitive C reaction protein (WMD=?5.66, 95% CI (?8.01, ?3.31); low-quality evidence). Sensitivity, subgroup, and meta-regression analyses were also assessed. Conclusion: The study demonstrated that CDDP has beneficial clinical effects for treating NPDR and improve the vision. Moreover, it indicated that oral CDDP in NPDR patients led to significant regulation of serum level of fasting blooding glucose, hemoglobin A1c and high sensitive C reaction protein, which was associated with the pathogenesis of NPDR. However, high-quality and large randomized clinical trials will be needed to prove the consequence in future.     

HPLC法测定鼻炎丸中黄芩苷的含量

目的建立高效液相色谱法测定鼻炎丸中黄芩苷的含量.方法高效液相色谱法条件:色谱柱以十八烷基硅烷键合硅胶为填充剂;以甲醇-水-磷酸(47∶53∶0.2)为流动相;检测波长为280nm.结果黄芩苷在进样量为0.204～1.02μg(r=0.9999)范围内与峰面积呈良好的线性关系;测定本品中黄芩苷的含量,加样回收率为99.97%(n=6),RSD为0.70%.结论所建立的方法准确可行,重复性好,可有效控制鼻炎丸的质量.     

高效液相色谱法测定全鹿丸中齐墩果酸含量

目的建立测定全鹿丸中齐墩果酸含量的高效液相色谱法。方法色谱柱采用Phenomenex C18柱(250 mm×4.6 mm,5μm),以0.045%冰醋酸溶液(用三乙胺调节pH至4.0)-乙腈(12∶88)为流动相,流速为1.0 mL/min,检测波长为210 nm,进样量为10μL,柱温为30℃。结果齐墩果酸进样量在0.268 1～8.936μg范围内与峰面积呈良好线性关系(r=0.999 8),平均加样回收率为100.26%,RSD为1.56%(n=9)。结论该方法测定全鹿丸中齐墩果酸的含量,方法简便、准确,结果稳定,可为全鹿丸的质量评价提供依据。     

固相萃取-离子色谱法测定四神丸中氯离子含量

目的建立离子色谱法测定四神丸中氯离子含量的方法,以评价制剂中补骨脂的炮制情况。方法样品水提液经固相萃取技术净化后,用离子色谱法测定氯离子含量。色谱柱为IonPacASl9柱（250mm×4mna,5μm）,淋洗液为KOH溶液梯度洗脱,检测器为电导检测器（EcD）,抑制器电流为25mA。结果氯离子质量浓度线性范围是0．001～0．051g／L,平均回收率为99．75％,RSD=5．49％（n=9）。结论该方法简便准确,能有效评价四神丸的质量。     

二十五味珊瑚丸中马兜铃酸A含量研究

目的:建立二十五味珊瑚丸中马兜铃酸A含量的HPLC测定方法,用于市场上二十五味珊瑚丸中马兜铃酸A的定性和定量检测。方法:采用kromasil C18(4.6×200mm),甲醇-0.05%冰醋酸(62:38)作为流动相,柱温30℃,流速1mL/min,250nm波长下检测。结果:马兜铃酸A在0.052mg/mL-0.0936mg/mL范围内线性关系良好,r=0.9999。平均回收率为99.67%,RSD为1.33%。结论:以高效液相色谱法检测二十五味珊瑚丸中马兜铃酸A的方法简便、快速、准确、灵敏度高,重复性良好。