Cardioprotective effect of Sida rhomboidea. Roxb extract against isoproterenol induced myocardial necrosis in rats

The present study investigates cardioprotective effect of Sida rhomboidea. Roxb (SR) extract on heart weight, plasma lipid profile, plasma marker enzymes, lipid peroxidation, endogenous enzymatic and non-enzymatic antioxidants and membrane bound ATPases against isoproterenol (IP) induced myocardial necrosis (MN) in rats. Rats treated with IP (85 mg/kg, s.c.) recorded significant (p<0.05) increment in heart weight, plasma lipid profile, plasma marker enzymes of cardiac damage, cardiac lipid peroxidation (LPO) and activity levels of Ca⁺² ATPase whereas there was significant (p<0.05) decrease in plasma HDL, cardiac endogenous enzymatic and non-enzymatic antioxidants, Na⁺-K⁺ ATPase and Mg⁺² ATPase. Pre-treatment with SR extract (400 mg/kg per day, p.o.) for 30 consecutive days followed by IP injections on days 29th and 30th, showed significant (p<0.05) decrease in heart weight, plasma lipid profile, plasma marker enzymes of cardiac damage, cardiac lipid peroxidation, Ca⁺² ATPase and significant increase in plasma HDL, cardiac endogenous enzymatic and non-enzymatic antioxidants, Na⁺-K⁺ ATPase and Mg⁺² ATPase compared to IP treated group. Hence, this study is the first scientific report on cardioprotective effect of SR against IP induced MN in rats.     

Clinicoimmunologic studies on Phoenix sylvestris Roxb. pollen: an aeroallergen from Calcutta, India

BACKGROUND: This study highlights the allergenicity and allergenic components of the pollen of Phoenix sylvestris Roxb. (PS), or date sugar palm, which is predominantly airborne in the air of Greater Calcutta. METHODS: A 2-year aerobiologic survey was performed by Burkard sampler. PS pollen extract was used in skin tests of allergic patients, fractionated by (NH4)2SO4 and the Sephacryl S-200 column. The allergenicity of each fraction was checked by skin test and IgE ELISA inhibition. The principal allergenic fraction, Fr.lla, was separated in 11% SDS-PAGE, and its allergenicity was confirmed by IgE ELISA inhibition and immunoblotting. RESULTS: PS pollen grains were found to be prevalent in the air of the suburban zone of Calcutta from January to March with a peak in February. The pollen extract showed high (44.07%) positive skin reaction on 540 respiratory allergic patients. Among the (NH4)2SO4 cut fractions, Fr.II was the most active one, and it was resolved into four subfractions in the Sephacryl S-200 column. Fr.lla was the principal allergenic fraction, showing the presence of two components of 33 and 66 kDa in SDS-PAGE. In IgE immunoblotting, both of the components were found to be allergenic. CONCLUSIONS: The PS pollen grain is an important aeroallergen from Calcutta, India. The 33- and 66-kDa components are the major allergens present in the relevant pollen extract.     

闽产九节茶浸膏的质量分析

目的探讨建立福建三明产九节茶浸膏的质量控制方法。方法采用HPLC法测定九节茶浸膏中异嗪皮啶、迷迭香酸的含量。结果异嗪皮啶、迷迭香酸在0.005～0.199μg、0.008～0.334μg范围内具有良好的线性关系;平均加样回收率分别为96.45%(RSD=2.79%,n=6),97.70%(RSD=1.82%,n=6)。结论为制定闽产九节茶浸膏的质量控制方法提供了实验依据。     

肿节风浸膏溶液对鼻咽癌细胞系CNE-2的放射增敏作用

目的：探讨肿节风浸膏对鼻咽癌细胞系CNE-2的细胞毒性作用以及对放射敏感性的影响。方法：应用克隆形成方法,观察不同浓度的肿节风浸膏对CNE-2的细胞杀灭效应,求得IC50,选择合适浓度的肿节风浸膏配合照射和单纯照射对细胞的杀伤作用,计算细胞存活率,用多靶单击数学模型进行曲线拟合作图。结果：与照射配合的肿节风浸膏的合适浓度为10mg／L。单纯照射组D0值为3．096Gy,照射加肿节风浸膏组D0值为2．441Gy,放射增敏比（SER）为1．268（3．096／2．441）。结论：肿节风浸膏具有一定的放射增敏作用。     

肿节风中咖啡酸衍生物及抗氧化活性

目的对肿节风中抗氧化活性成份进行分离鉴定和抗氧化活性测定。方法利用各种色谱技术进行分离纯化,利用波谱数据鉴定结构,采用DPPH自由基清除率法测定抗氧化活性。结果从肿节风中进一步得到4个化合物,分别鉴定为:4-O-咖啡酰基-奎宁酸(4-O-caffeoylquinic acid,1),3-O-咖啡酰基-奎宁酸(3-O-caffeoylquinic acid,2),4-O-咖啡酰基-奎宁酸甲酯(4-O-caffeoylquinic acidmethyl ester,3),3-O-咖啡酰基-奎宁酸甲酯(3-O-caffeoylquinic acid methyl ester,4),各化合物显示了不同强度的抗氧化活性。结论这4个化合物均为首次从该属中分离得到,各咖啡酸衍生物、丹参素衍生物,具有邻二酚羟基的黄酮类化合物是肿节风抗氧化活性部位的物质基础。     

HPLC法测定两面针中氯化两面针碱的含量

建立了两面针中氯化两面针碱的HPLC测定法，方法简便、准确。加样回收率为97.7％，RSD＝0．82％，最低检测浓度0．8μg／mL，可用于两面针的质量控制。     

土茯苓化学成分研究(IV)

Smilax glabra is a wellknown traditional Chinese medicine which has been used clinically to prevent leptospirosis, to treat syphilis, and acute bacterial dysentery, etc. Its extracts showed antitumor and antiatherosclerosis activiy. A new isoflavone, 7,6′-dihydroxy 3′-methoxy isoflavone (1), along with two known compounds taxifolin (2) and astilbin (3), have been isolated from the roots of Smilax glabra. The structures were elucidated by spectroscopic methods including 2DNMR techniques.     

HPLC法测定草珊瑚中富马酸含量的方法学研究

目的:建立高效液相色谱测定草珊瑚中富马酸含量的方法。方法:采用高效液相色谱法,固定相为Dia-monsil C18柱(5μm,250×4.6mm),以乙腈-水-磷酸(2:98:0.05)作为流动相,检测波长为208nm。结果:富马酸在54.35~434.8μg范围内线性关系良好,r2=0.9999,平均回收率为99.95%,RSD=1.33%。结论:该方法操作简单、准确,重复性好,可适用于草珊瑚药材的质量控制。     

土茯苓祛痰止咳作用药理实验研究

目的：研究土茯苓对浓氨气引起小鼠咳嗽次数与咳嗽潜伏期的影响,以及对小鼠腹腔注射酚红后引起气管酚红排泌量的影响。方法：将小鼠放入自制的诱咳仪内,注入浓氨气20s,观察记录小鼠的咳嗽潜伏期和3min内咳嗽次数;小鼠腹腔注射5％酚红溶液,测定30min后小鼠气管酚红的排泌量。结果：各组咳嗽潜伏期以及3min内咳嗽次数比较无显著性差异（P〉0．05）,土茯苓高剂量组（10g／kg）的吸光度高于空白对照组,差异有显著性（P〈0．05）。结论：单味土茯苓止咳作用尚不明显,但在高剂量时有明显祛痰作用。     

广枣总黄酮对阿霉索引起大鼠心肌过氧化损伤的保护作用

目的　通过广枣总黄酮 (TFCA)对阿霉素 (ADR)引起大鼠心肌过氧化损伤保护作用的研究 ,进一步证实广枣疗效可能与 TFCA抗氧化作用有关。方法　动物分为空白组、ADR组和实验组 (ADR TFCA10 0 ,15 0 ,2 0 0mg/ kg组 )。以“心酶谱”、抗氧化酶做为检测指标。结果　 ADR组心酶谱、MDA均比空白组高 ,而抗氧化酶活性均比空白组低。TFCA干预后“心酶谱”、MDA下降 ,抗氧化酶活性上升。结论　 TFCA能抑制 ADR所引起大鼠心肌过氧化损伤