土茯苓的化学成分研究（一）

从土茯苓中分得２个单体化合物，经光谱分析分别鉴定为３，５，４’－三羟基芪（Ｉ）和（一）表儿茶精（Ⅱ），这两个化合物系首次从该植物中分离得到。并通过２维核磁共振相关谱准确地指定了这两个化合物的碳氢归属。     

Antibacterial activity of Ailanthus excelsa (Roxb)

The antibacterial activity of different fractions of a methanol extract obtained from the dried stem bark of Ailanthus excelsa (Roxb) was studied using different bacterial strains. The ethyl acetate fraction inhibited the growth of all test bacteria. The MIC of the EA fraction was found to be 6 mg/disc. Copyright -Copyright 2001 John Wiley & Sons, Ltd.     

破骨风化学成分的研究

 目的研究破骨风的化学成分。方法破骨风全草用体积分数为95%乙醇提取物的石油醚、氯仿和乙酸乙酯部分进行色谱分离得到8个化合物,通过波谱解析和理化性质进行结构鉴定。结果分离鉴定了8个化合物:白桦脂醛(1),β-谷甾醇(2),白桦脂酸(3),齐墩果酸(4),白桦脂醇(5),胡萝卜苷(6),jasminoside(7),丁香苷(8)。结论除化合物7之外,其余7个化合物皆为首次从该植物中分离得到,化合物8为首次从该属植物中分离得到。     

土茯苓及其制剂中落新妇甙含量测定

采用紫外分光光度法对土茯苓中落新妇甙含量进行测定。实验结果表明,土茯苓中落新妇甙的平均含量为０．０２７５％,而银屑冲剂（含土茯苓）则未测出落新妇。关键词##4土茯苓;;落新妇甙;;紫外分光光度法;;银屑冲剂     

Clinicoimmunologic studies on Phoenix sylvestris Roxb. pollen: an aeroallergen from Calcutta, India

BACKGROUND: This study highlights the allergenicity and allergenic components of the pollen of Phoenix sylvestris Roxb. (PS), or date sugar palm, which is predominantly airborne in the air of Greater Calcutta. METHODS: A 2-year aerobiologic survey was performed by Burkard sampler. PS pollen extract was used in skin tests of allergic patients, fractionated by (NH4)2SO4 and the Sephacryl S-200 column. The allergenicity of each fraction was checked by skin test and IgE ELISA inhibition. The principal allergenic fraction, Fr.lla, was separated in 11% SDS-PAGE, and its allergenicity was confirmed by IgE ELISA inhibition and immunoblotting. RESULTS: PS pollen grains were found to be prevalent in the air of the suburban zone of Calcutta from January to March with a peak in February. The pollen extract showed high (44.07%) positive skin reaction on 540 respiratory allergic patients. Among the (NH4)2SO4 cut fractions, Fr.II was the most active one, and it was resolved into four subfractions in the Sephacryl S-200 column. Fr.lla was the principal allergenic fraction, showing the presence of two components of 33 and 66 kDa in SDS-PAGE. In IgE immunoblotting, both of the components were found to be allergenic. CONCLUSIONS: The PS pollen grain is an important aeroallergen from Calcutta, India. The 33- and 66-kDa components are the major allergens present in the relevant pollen extract.     

闽产九节茶浸膏的质量分析

目的探讨建立福建三明产九节茶浸膏的质量控制方法。方法采用HPLC法测定九节茶浸膏中异嗪皮啶、迷迭香酸的含量。结果异嗪皮啶、迷迭香酸在0.005～0.199μg、0.008～0.334μg范围内具有良好的线性关系;平均加样回收率分别为96.45%(RSD=2.79%,n=6),97.70%(RSD=1.82%,n=6)。结论为制定闽产九节茶浸膏的质量控制方法提供了实验依据。     

白饭树水提取物对肝纤维化大鼠血清学的影响

目的:研究白饭树水提取物对肝纤维化(HF)大鼠血清学的影响。方法:采用皮下注射(SC)四氯化碳(CCl4)油溶液造成HF模型,以秋水仙碱(colchicine)为阳性对照,观察白饭树水提取物对血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)含量的影响。结果:白饭树水提取物能降低HF模型大鼠血清中ALT、AST含量,提升ALB含量,并且有效降低HF模型大鼠血清中HA、LN、PCⅢ的含量,高剂量组总体效果优于低剂量组。结论:白饭树水提取物对HF大鼠有保肝降酶、改善蛋白脂质、抗HF作用,值得深入研究。     

HPLC法测定独子藤中雷公藤红素的含量

目的建立高效液相色谱法测定独子藤中雷公藤红素的含量,为雷公藤红素的药用植物资源开发提供依据。方法采用Supelco Discovery C18柱（25 cm×4.6 mm,5μm）,流动相为甲醇-5%（体积分数）H3PO4水溶液（体积比80∶20）,流速为1.0 mL.min-1,检测波长为426 nm。结果雷公藤红素在40～200μg.mL-1范围内与峰面积呈良好线性关系（r=0.999 1）。独子藤中雷公藤红素的平均质量分数为0.055 5%,雷公藤红素的平均回收率为97.6%,RSD=1.78%（n=6）。结论该方法简便、准确、灵敏度高、重现性好,可用于独子藤中雷公藤红素的含量测定;独子藤中雷公藤红素含量较高,可作为雷公藤红素的资源植物加以开发利用。     

两面针中木脂素化合物结晶-8对伤害性疼痛大鼠c-fos基因表达的影响

摘要目的研究两面针中木脂素化合物结晶8（简称结晶8）对甲醛所致伤害性疼痛大鼠的即早基因（c fos）表达的影响。方法SD雌性大鼠34只,随机分为5组：0.9%氯化钠溶液组、模型组、阳性对照组（罗通定注射液2 mg·kg－1）、结晶 8高剂量（2 mg·kg－1）组各7只,结晶 8低剂量（1 mg·kg－1）组6只。采用免疫组化染色观察疼痛大鼠c fos基因的表达情况。结果模型组大鼠脑干、下丘脑及脊髓视野中Fos阳性颗粒分别为（20.33±2.63）,（19.28±2.42）,（18.36±5.11）颗;结晶 8高剂量组分别为（10.76±3.66）,（12.49±2.80）,（6.87±2.10）颗。结论结晶 8的镇痛机制可能与抑制中枢FOS蛋白的表达有关。     

Cardioprotective effect of Sida rhomboidea. Roxb extract against isoproterenol induced myocardial necrosis in rats

The present study investigates cardioprotective effect of Sida rhomboidea. Roxb (SR) extract on heart weight, plasma lipid profile, plasma marker enzymes, lipid peroxidation, endogenous enzymatic and non-enzymatic antioxidants and membrane bound ATPases against isoproterenol (IP) induced myocardial necrosis (MN) in rats. Rats treated with IP (85 mg/kg, s.c.) recorded significant (p<0.05) increment in heart weight, plasma lipid profile, plasma marker enzymes of cardiac damage, cardiac lipid peroxidation (LPO) and activity levels of Ca⁺² ATPase whereas there was significant (p<0.05) decrease in plasma HDL, cardiac endogenous enzymatic and non-enzymatic antioxidants, Na⁺-K⁺ ATPase and Mg⁺² ATPase. Pre-treatment with SR extract (400 mg/kg per day, p.o.) for 30 consecutive days followed by IP injections on days 29th and 30th, showed significant (p<0.05) decrease in heart weight, plasma lipid profile, plasma marker enzymes of cardiac damage, cardiac lipid peroxidation, Ca⁺² ATPase and significant increase in plasma HDL, cardiac endogenous enzymatic and non-enzymatic antioxidants, Na⁺-K⁺ ATPase and Mg⁺² ATPase compared to IP treated group. Hence, this study is the first scientific report on cardioprotective effect of SR against IP induced MN in rats.