Antiobesity effects of the water-soluble fraction of the ethanol extract of Smilax china L. leaf in 3T3-L1 adipocytes

BACKGROUND/OBJECTIVESSeveral medicinal properties of Smilax china L. have been studied including antioxidant, anti-inflammatory, and anti-cancer effects. However, the antiobesity activity and mechanism by which the water-soluble fraction of this plant mediates its effects are not clear. In the present study, we investigated the lipolytic actions of the water-soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) in 3T3-L1 adipocytes.MATERIALS/METHODSThe wsSCLE was identified by measuring the total polyphenol and flavonoid content. The wsSCLE was evaluated for its effects on cell viability, lipid accumulation, glycerol, and cyclic adenosine monophosphate (cAMP) contents. In addition, western blot analysis was used to evaluate the effects on protein kinase A (PKA), PKA substrates (PKAs), and hormone-sensitive lipase (HSL). For the lipid accumulation assay, 3T3-L1 adipocytes were treated with different doses of wsSCLE for 9 days starting 2 days post-confluence. In other cell experiments, mature 3T3-L1 adipocytes were treated for 24 h with wsSCLE.RESULTSResults showed that treatment with wsSCLE at 0.05, 0.1, and 0.25 mg/mL had no effect on cell morphology and viability. Without evidence of toxicity, wsSCLE treatment decreased lipid accumulation compared with the untreated adipocyte controls as shown by the lower absorbance of Oil Red O stain. The wsSCLE significantly induced glycerol release and cAMP production in mature 3T3-L1 cells. Furthermore, protein levels of phosphorylated PKA, PKAs, and HSL significantly increased following wsSCLE treatment.CONCLUSIONThese results demonstrate that the potential antiobesity activity of wsSCLE is at least in part due to the stimulation of cAMP-PKA-HSL signaling. In addition, the wsSCLE-stimulated lipolysis induced by the signaling is mediated via activation of the β-adrenergic receptor.     

顶空固相微萃取法与水蒸气蒸馏法联合气相色谱-质谱分析肿节风挥发性成分

目的：比较顶空固相微萃取法（HS-SPME）与水蒸气蒸馏法（SD）提取肿节风中挥发性成分的差异。方法：采用气相色谱-质谱（GC-MS）联用技术分析肿节风挥发性成分。结果：采用HS-SPME-GC-MS共分离出59个色谱峰,鉴定出40个化合物,占总挥发性成分的88.47%;SD-GC-MS共分离出59个色谱峰,鉴别出38个化合物,占总挥发性成分的90.04%;2种不同前处理技术共同鉴定出的挥发性成分有11种。结论：HS-SPME与SD联合GC-MS适用于分析不同类型挥发性化学成分,将两种方法联用分析肿节风挥发性成分能够得到更加全面的信息。     

Hypokaliémie avec pseudo-hyperaldostéronisme : est-ce un syndrome de Lidl® ?

IntroductionIntoxication induced by glycyrrhizin is a common cause of hypokalaemia by pseudo-hyperaldosteronism.ObservationWe hereby present the observation of a 68-year old patient hospitalised following a full hip-prosthesis operation after a deep hypokalaemia at 2.5mM was observed, with ECG signs (flat T waves and appearance of U waves). The kaliuresis was not adapted at 8,4mmol/mmol of creatininuria. We noted a history of axonal and demyelinising polyneuropathy, of psoriasis and chronic ethylism.The evolution after intravenous potassic supplementation and then per os was favourable leading to a normalisation of the blood and urinary potassic concentrations. The blood concentrations of renin and of aldosterone upon admission were lower than the detection threshold and the tests carried out 7 days later were normal with a plasmatic renin of 35.2 pg/mL and a plasmatic aldosterone of 74 pg/mL, therefore indicating a toxic cause. It is the interview of the patient that allowed for the diagnosis, identifying a daily, prolonged and important consumption (around 1L every 2-3 days for several years) of a pastis produced by supermarket brand Lidl®. The composition of the drink mentions ‘liquorice infusion’ without giving any more information as regards to the real concentration; it was later estimated at 170 mg/L by the distributor.DiscussionThe consumption of glycyrrhizin is a well-known aetiology for pseudo-hyperaldosteronism. It is commonly mentioned amongst excessive consumers of liquorice or of non-alcoholic anise drinks. Drinks that are derived from original pastis contain varying levels of glycyrrhizin, which is used as a flavour enhancer and can become toxic in cases of prolonged and important consumption.     

一起因误食商陆叶引起的食物中毒事件的调查

目的及时查清1起食物中毒事件发生原因,探讨致病因素,为预防类似事件提供参考。方法现场流行病学调查、简易动物毒性实验。结果6人进食商陆叶后全部发病,其中重症5例,经治疗后痊愈,无死亡病例。经调查确定为商陆叶引起的急性食物中毒事件。结论商陆为有毒植物,食用不当可引起食物中毒,此次事件发生的主要原因是当地群众对商陆的认识不足,加强宣传教育是防范类似事件发生的关键。     

Pharmacological Studies on the Effect of the Treatment of Swiss A Mice with Diospyrin,a Tumour-inhibitory Plant Product,and its Synthetic Derivatives

Diospyrin, a bisnaphthoquinonoid plant product and its derivatives have shown significant inhibitory activities against murine tumours in vivo . Studies on the haematological status, serum protein and creatinine levels, activities of several serum glycolytic enzymes, and histopathology of the mice inoculated with Ehrlich ascites carcinoma were carried out after treatment with diospyrin and four synthetic derivatives. The prognostic significance of the pharmacological parameters acting as markers of the diseased state was evident from these findings. Normal mice were also studied before and after treatment with these compounds which did not cause noticeable adverse effects on the vital parameters, thereby indicating the possibility of the utilization of diospyrin and derivatives as appropriate therapeutic agents.     

黔产红土茯苓药材质量标准初步研究

目的:建立黔产红土茯苓药材的质量标准。方法:对不同产地黔产红土茯苓药材进行性状鉴别、显微鉴别、薄层色谱鉴别;参照《中国药典》2015年版四部通则方法对黔产红土茯苓药材水分、总灰分、浸出物进行测定。结果:薄层色谱斑点清晰,分离好,专属性强,无干扰;初步规定黔产红土茯苓药材水分不得过15.0%,总灰分不得过5.0%,水溶性浸出物含量不得少于8.0%。结论:该方法操作简便、结果可靠,能够为黔产红土茯苓药材质量评价提供一定的参考。     

高效液相色谱-串联质谱法分析破骨风中裂环环烯醚萜苷类成分

目的：利用高效液相色谱-电喷雾串联质谱法（HPLC—DAD—ESI—MS^2）分析了破骨风中裂环环烯醚萜苷类成分。方法：采用该方法得到了破骨风中裂环环烯醚萜苷类成分的紫外（UV）色谱图、总离子流（TIC）色谱图和萃取离子（EIC）色谱图,以及相应色谱峰的紫外光谱图和EIC／MS^2的质谱图,结合文献对其进行解析。结果：鉴别出破骨风中6个裂环环烯醚萜苷类成分,分别为Jasminoside,Jaslanceoside A,Jaslanceoside B,Jaslanceoside C,Jaslanceoside D和Jaslanceoside E;同时发现还有4个未知结构裂环环烯醚萜苷类化合物存在,并对它们的结构进行了推测。结论：高效液相色谱-电喷雾串联质谱法可以用于分析破骨风中裂环环烯醚萜苷类成分。     

HPLC法测定草珊瑚中富马酸含量的方法学研究

目的:建立高效液相色谱测定草珊瑚中富马酸含量的方法。方法:采用高效液相色谱法,固定相为Dia-monsil C18柱(5μm,250×4.6mm),以乙腈-水-磷酸(2:98:0.05)作为流动相,检测波长为208nm。结果:富马酸在54.35~434.8μg范围内线性关系良好,r2=0.9999,平均回收率为99.95%,RSD=1.33%。结论:该方法操作简单、准确,重复性好,可适用于草珊瑚药材的质量控制。     

银苓Ⅰ号对铅中毒小鼠非特异免疫功能的影响

目的:探讨中药复方银苓Ⅰ号对铅中毒小鼠非特异免疫功能的影响。方法:小鼠自由饮用醋酸铅溶液造模,分别予银苓Ⅰ号或依地酸钠钙治疗3周;观察外周血弊细胞计数、碳粒廓清指数K及校正指数α、免疫脏器指数等非特异性免疫功能指标的变化。结果:银苓Ⅰ号对外周血白细胞计数、碳粒廓清指数K及校正指数α、免疫脏器指数有明显改善作用,而且碳粒廓清能力优于依地酸钠钙。结论:银苓号可在一定程度上对抗铅中毒所致的非特异免疫损害,效果优于依地酸钠钙。     

中药九节茶提取物对应激负荷小鼠免疫功能的改善作用

目的:研究中药九节茶对束缚应激小鼠免疫功能的改善作用.方法:实验将雄性C_(57)BL/6小鼠分为正常对照组,应激对照组,125,500 mg·kg~(-1)九节茶提取物给药组,制备脾脏淋巴细胞悬液,流式细胞术分析T淋巴细胞亚群、NK细胞和NKT细胞比例及数目.结果:与应激对照组相比,中药九节茶可以提高束缚应激小鼠脾脏淋巴细胞总数,改善T淋巴细胞亚群比例,同时增加脾脏NK细胞和NKT细胞比例及数目.结论:中药九节茶可以改善应激负荷小鼠淋巴细胞数目及淋巴细胞亚群比例.