A strategy for rapid discovery of traceable chemical markers in herbal prod-ucts using MZmine 2 data processing toolbox: A case of Jing Liqueur

Objective: The quality evaluation of herbal products remains a big challenge. Traceable markers are the core concept of the authentication of herbal products. However, the discovery of traceable markers is labor-intensive and time-consuming. The aim of this study is to develop a convenient approach to rapidly screen the traceable markers for herbal product authentication. Methods: Commercial Jing Liqueur and its 22 species of herbal ingredients were analyzed using HPLC-QTOF-MS and GC-MS to characterize nonvolatile and volatile chemicals. The acquired data were imported into MZmine 2 software for mass detection, chromatogram building, deconvolution and alignment. The aligned data were exported into a csv file and then traceable markers were selected using the built-in filter function in Excel. Finally, the traceable markers were identified by searching against online databases or publications, some of which were confirmed by reference standards. Results: A total of 288 chemical features transferred from herbal materials to Jing Liqueur product were rapidly screened out. Among them, 52 markers detected by HPLC-QTOF-MS were annotated, while nine volatile markers detected by GC-MS were annotated. Moreover, 30 of these markers were confirmed by comparing with reference standards. A chemical fingerprint consisting of traceable markers was finally generated to ensure the authentication and quality consistency of Jing Liqueur. Conclusion: A strategy for rapid discovery of traceable markers in herbal products using MZmine 2 software was developed.     

针灸名家指力练习的启示

针刺基本功是提高手法操作、取得临床疗效的关键环节,其主要内容就是指力。指力需要掌握恰当的方法,勤学苦练才能获得。笔者结合针灸名家贺普仁、彭静山及个人领悟,介绍指力的练习方法。     

盆炎净胶囊中忍冬藤药材马钱苷的定性与定量分析

目的:建立盆炎净胶囊中忍冬藤药材马钱苷的定性与定量检测方法,并制定质量标准。方法:采用薄层色谱法,以忍冬藤对照药材、马钱苷为参照,以乙酸乙酯-冰醋酸-水(12∶3∶5)上层溶液为展开剂,建立其定性鉴别方法;采用高效液相色谱法,以马钱苷为对照,WatersT3 C18(4.6 mm×250 mm,5μm)为色谱柱,乙睛-0.5%醋酸水溶液(13∶87)为流动相,流速1.0 mL/min,检测波长为235 nm,柱温25℃,测定盆炎净胶囊中马钱苷的含量。结果:TLC法斑点清晰,分离效果好;马钱苷在3.66~32.90μg/mL范围内呈良好的线性关系,r=0.9998;平均回收率为100.08%、RSD为0.22%。结论:本法简捷、快速、可行、重复性好,可作为盆炎净胶囊中忍冬藤药材马钱苷的质量控制提供依据。     

生津颗粒治疗消渴病的临床和实验研究

目的 探讨生津颗粒治疗消渴病的临床和实验疗效。方法 用生津颗粒治疗３０例阴虚热盛消渴病患者，总有效率达７６．７％，结果 生津颗粒有较明显的降糖作用。结论生津颗粒对消渴病患者有较好疗效，降血糖作用明显，且有价廉。口感好，服用方便，药源丰富等优点。     

自然病理实验揭示血管球体与井穴和宣穴高度相关

十二井穴与十宣穴虽然属于人体不同的经络和部位，但都有相同的清热、醒神等特殊作用，提示这些穴位可能有相似的解剖结构和针灸靶点。本研究首次采用了“针灸自然病理实验”（NPEA）的方法探索针灸原理，提出了“证实解剖结构与针灸靶点相关的标准”。通过分析人体手足常见的15种软组织肿瘤，发现血管球瘤（Glomus tumor）的分布与井宣穴相关性最高。对近10年中国知网中671例血管球瘤病报告分析表明，发生在手足的占比87%；在手足血管球瘤中，发生在甲下及甲旁肿瘤占比85%，与井宣穴的分布高度一致。结果提示产生血管球瘤的正常解剖结构，血管球体（Glomus body）可能是针灸的靶点。支持这个假说的证据包括，血管球体的解剖分布、生理功能、病理变化及临床特征等均同井宣穴高度相关。目前有关人体血管球体的分子生物学原理所知甚少，深入研究不但会有益于解释体温和血液循环的周围调节机制，还可能发现针灸井穴和宣穴的特异靶点和机制，进而提高临床治疗的效果。