日龄及性别对行光疗的新生儿高胆红素血症患儿血清总胆红素水平的影响

目的探讨日龄及性别对行光疗的新生儿高胆红素血症患儿血清总胆红素水平的影响。方法选取2019年1-12月医院新生儿科收治的232例行光疗的新生儿高胆红素血症患儿作为研究对象,比较不同日龄、不同性别患儿光疗前后的血清总胆红素水平变化。结果光疗后,不同日龄、不同性别患儿的血清总胆红素水平均低于光疗前,差异有统计学意义(P<0.05);不同日龄患儿光疗后的血清总胆红素水平降低程度比较,差异有统计学意义(F=3.871,P=0.022),其中日龄5~10 d患儿的血清总胆红素水平降低程度最为显著;不同性别患儿的血清总胆红素水平降低程度比较,差异无统计学意义(P>0.05)。结论出生5~10 d时,光疗干预可更有效降低新生儿高胆红素血症患儿的血清总胆红素水平,利于疾病好转,而性别差异对光疗效果影响不大。     

妊娠期甲减患者孕晚期鸢尾素水平与机体代谢指标及母婴妊娠结局的相关性分析

目的 分析妊娠期甲减患者孕晚期体内血清鸢尾素水平与机体代谢指标及母婴不良妊娠结局之间的关系。方法 研究纳入2017年5月—2018年12月共120例孕晚期甲减患者,将其分为高鸢尾素组（60例）与低鸢尾素组（60例）,同时选取100例同孕期甲状腺功能正常的孕妇作为对照。记录相关血液代谢指标和妊娠结局及胎儿或新生儿相关并发症。结果 高鸢尾素妊娠期甲减患者体重指数大于低鸢尾素组（P＜0.05）;促甲状腺激素（TSH）、甲状腺过氧化物酶抗体（TPO - Ab）高于低鸢尾素组（P＜0.05）;游离甲状腺素（FT4）低于低鸢尾素组（P＜0.05）;低密度脂蛋白胆固醇(LDL - C)、总胆固醇(TC)、三酰甘油(TG)高于低鸢尾素组（P＜0.05）;高密度脂蛋白胆固醇(HDL - C)低于低鸢尾素组（P＜0.05）。妊娠期甲减患者血清鸢尾素水平与TSH正相关（r = 0.329,P＜0.05）,而与FT4负相关（r = - 0.286,P＜0.05）。高鸢尾素妊娠期甲减患者产后出血发生率高于低鸢尾素组（P＜0.05）。同样,新生儿低体重、新生儿呼吸窘迫综合征的发生率也高于低鸢尾素组（P＜0.05）。结论 血清鸢尾素水平可反映妊娠期甲减患者病情严重程度。母婴妊娠相关并发症发生率在高鸢尾素妊娠甲减患者中较高。     

子痫前期患者血清AFP、D-二聚体、PLGF水平对母体及妊娠结局影响

目的:分析子痫前期(PE)患者血清甲胎蛋白(AFP)、D-二聚体(D-dimer)及胎盘生长因子(PLGF)水平对母体及妊娠结局的影响。方法:将本院2016年1月-2018年9月收治的330例PE患者分轻度PE组(n=117)、重度PE组(n=213),另选同期本院住院待产的正常妊娠产妇30例为对照组,检测各组孕妇血清AFP、D-dimer、PLGF水平,绘制受试者工作特征(ROC)曲线分析对PE的诊断预测效能;Spearman相关性分析各检测指标与病情的相关性;对PE患者妊娠结局的预测效能。结果:PE组与对照组AFP比较无差异(P>0.05),但D-dimer水平PE组高于对照组,PLGF低于对照组(P<0.05);D-dimer AUC值最高(387.51μg/L),预测PE的敏感度、特异度为80.6%、100.0%;PLGF AUC值次之(217.33pg/ml),预测PE的敏感度为96.7%,高于D-dimer。不同病情严重程度的PE患者血清AFP、PLGF水平未见差异(P>0.05),但D-dimer轻度PE组低于重度PE组(P<0.05)。AFP与PE病情程度未见相关性(r=0.093,P=0.078),D-dimer与PE病情程度正相关(r=0.796,P=0.000),PLGF与PE病情程度负相关(r=0.114,P=0.031)。PE患者中,母体发生并发症、胎儿结局不良者血清D-dimer水平高于无并发症、胎儿结局良好者(P<0.05),AFP、PLGF未见差异(P>0.05);D-dimer对PE患者并发症可发挥一定预测效能,以579.53μg/L为cut-off,预测PE患者并发症的敏感度、特异度为80.9%、61.9%,另两项血清指标对妊娠结局的预测效能不佳。结论:血清AFP、D-dimer、PLGF3个指标中,D-dimer对PE的预测价值及其与PE病情的相关性最为显著,用于预测PE患者母体并发症具有一定敏感度,但特异度不佳。     

Detection of Mycoplasma pneumoniae in serum specimens from patients with mycoplasma pneumonia by PCR

There are few data on detection of Mycoplasma pneumoniae from blood, serum or plasma, and systematic studies on this diagnostic approach in community-acquired pneumonia (CAP) are scarce. Compared to testing respiratory specimens, this approach has the advantages that it is less dependent on proper specimen collection, serum is easily stored and handled, and the pathogen is detected in a primary sterile site, where colonization can be ruled out. In this study, acute-phase serum specimens from 29 patients of Vienna University Hospital (treated between 11/1994 and 6/2004; female: 14, male: 15; median age: 31 years, range: 15-66 years) with CAP and serologically verified M. pneumoniae infection, who had not received anti-mycoplasma therapy prior to serum collection, were tested for M. pneumoniae by conventional PCR and real-time PCR. Conventional PCR yielded negative results for all specimens, but real-time PCR detected M. pneumoniae in 15/29 patient sera (52%). These findings indicate that M. pneumoniae is present in the bloodstream of a substantial proportion of patients with mycoplasma pneumonia. Despite the possible adherence of M. pneumoniae to human erythrocytes, the pathogen can be detected from serum, if a method with enhanced sensitivity is applied. However, the negative predictive value of PCR from serum with regard to etiological diagnosis is low. With regard to the potential clinical benefit of blood-based PCR diagnosis of mycoplasma pneumonia the diagnostic accuracy of this approach using either serum or whole-blood specimens should be addressed by large-scale studies.     

Effect of acute exercise on some haematological parameters and neutrophil functions in active and inactive subjects

In this work we studied the possible effects of acute exercise on some haematological parameters and on some functions of neutrophils in seven active and six inactive subjects. Physical exercise (10 min on a cycle ergometer at a heart rate of 150 beats · min^–1) induced a significant increase in total leucocyte, lymphocyte and neutrophil concentrations in active subjects; serum iron and ferritin concentrations were lower in active compared to inactive subjects. Cellular adhesion, bactericidal activity and superoxide anion production did not change after exercise, while we also observed some differences between active and inactive subjects before exercise. In particular, the neutrophils from active subjects showed a significantly higher percentage of adhesion, higher bactericidal activity and lower superoxide anion production. In conclusion, the training induced changes in some neutrophil functions, while acute exercise influenced, overall, leucocyte concentrations.     

皮肤电导率在早期糖尿病肾病筛查中的应用价值

目的 探讨皮肤电导率（ESC）在早期糖尿病肾病筛查中的应用价值。方法 选取2022年10月至2023年2月于安徽医科大学第二附属医院分泌科住院的176例2型糖尿病患者,根据尿白蛋白/肌酐比值（UACR）和肾小球滤过率（eGFR）将其分为两组,糖尿病肾病组（DKD组,n=68）和糖尿病非肾病组（non-DKD组,n=108）,所有患者均完善SUDOSCAN仪检测,获得双手、双足ESC(HESC、FESC),肾病风险分数（SUDOSCAN-DN）,采用Spearman相关性分析UACR与SUDOSCAN各指标的关系。采用受试者工作特征（ROC）曲线判断SUDOSCAN各指标对糖尿病肾病的诊断效能。结果 与non-DKD组相比,DKD组年龄较大,病程较长,收缩压较高,三酰甘油（TG）、血尿酸及肌酐、促甲状腺激素（TSH）水平较高,HbA1c水平偏低,HESC、FESC、SUDOSCAN-DN值均较低,差异有统计学意义（P<0.05）;Spearman相关分析显示,UACR与TC、LDL-C、血肌酐、TSH呈正相关（r值分别为0.202、0.207、0.378、0.174,P<0....     

Serum vasopressin (AVP) levels in polyuric brain-dead organ donors

Summary Hydromineral metabolism and serum arginine-vasopressin (AVP) levels were investigated in 11 patients who sustained brain death. They showed various degrees of polyuria with low osmolality and low fractional sodium excretion. Urine osmolality was always below that of serum, and AVP levels were between 1.3 and 50.0 pg/ml vs 0.7–8.0 pg/ml in ten normal subjects. Thus central diabetes insipidus was excluded. A renal mechanism inducing water diuresis has to be assumed. The type of renal lesion, however, remains unclear.This work was supported by the Fonds zur Förderung der wissenschaftlichen Forschung, project no. 3100, Vienna     

Increased sodium requirement following early postnatal surgical correction of congenital uropathies in infants

Serum electrolyte equilibrium and plasma aldosterone concentrations were monitored in 19 infants who had severe obstructive uropathy or grade 5 vesico-ureteral reflux and were undergoing surgical correction in the first 2 months of life. Before surgery high plasma aldosterone levels were observed in 8 patients, but serum sodium and potassium concentrations were normal. Plasma concentrations of aldosterone were elevated in all patients during the week following surgery and 7 patients developed severe hyponatraemia, hyperkalaemia and weight loss despite very high plasma aldosterone concentrations. As a consequence 5 infants were infused with sodium chloride (4 mEq/kg per day) before and for 36h after surgery; this prevented metabolic imbalance. We conclude that infants undergoing surgical correction of uropathies may require a high sodium intake to maintain electrolyte balance and adequate growth.     

91例免疫相关性皮肤病患者血清花粉IgG抗体检测结果分析

目的：检测免疫相关性皮肤病患者血清花粉IgG阳性情况。方法：采用Dot-ELISA法检测受检患者血清花粉IgG抗体。结果：56％受检患者血 清花粉IgG抗体阳性,其中荨麻疹、湿疹、接触性皮炎和过敏性紫癜等过敏性皮肤病患者血清花粉IgG抗体阳性率为83．0％,其它免疫相关性皮肤病为18．4％（P＜0．005）。血清花粉IgG抗体阳性的34例荨麻疹患者中,早春花粉抗体阳性高达91．2％,晚春花粉52．9％,夏秋花粉29．4％（P＜0．005）。结论：致敏性花粉与过敏性皮肤病有较大关系。春季花粉,特别是早春花粉,是本地区花粉相关性荨麻疹的主要致敏花粉。