无疤玉润烧伤膏治疗烧烫伤的实验研究

目的:观察无疤玉润烧伤膏对烧烫伤的治疗效果。方法:采用家兔复制烧烫伤模型,观察无疤玉润烧伤膏对深Ⅱ度烫伤、浅Ⅱ度烧伤和Ⅲ度烧伤的影响;结果:创面应用无疤玉润烧伤膏可以缩短家兔深Ⅱ度烫伤、浅Ⅱ度烧伤和Ⅲ度烧伤烧伤创面愈合时间,与对照组比较有显著性差异(P<0.01);无疤玉润烧伤膏组较对照组病理损害明显减轻,疤痕减少。结论:无疤玉润烧伤膏具有改善烧伤创面皮肤的组织结构、促进创面愈合作用和减少疤痕形成作用。     

湿润烧伤膏对SD大鼠深Ⅱ度烫伤创面的影响及 机制探讨

目的　研究湿润烧伤膏对SD大鼠深Ⅱ度烫伤创面的影响及作用机制。方法　将60只SD大鼠制成深Ⅱ度烫伤模型,并按照随机数表法分为湿润烧伤膏组（30只）,创面外用湿润烧伤膏换药治疗;磺胺嘧啶银组（30只）,创面外用磺胺嘧啶银换药治疗。于烫伤后第3 天和第7天通过免疫组化法观察CD31、Ki67在创面上的表达,作为创面肉芽组织增生情况的评判指标;测定大鼠烫伤创面愈合率,作为判断创面愈合情况的指标;采用荧光定量PCR法测定两组大鼠血小板源性生长因子及表皮生长因子的表达量。结果　以愈合率达到95%为创面愈合,实验组创面平均愈合时间为17d,对照组创面平均愈合时间为20ｄ,两组创面愈合率进行配对t检验,P<0.05,差异具有统计学意义。免疫组化法观察结果显示,实验组CD31及Ki68的表达均强于对照组。Real-Time PCR 结果显示,实验组PDGF（血小板源性生长因子）在伤后1-7d的表达量均强于对照组;EGF（表皮生长因子)在伤后3-10d的表达量也均强于对照组。结论　湿润烧伤膏能通过增加PDGF、EGF的表达促进创面血管新生及细胞增殖,加速上皮化进程,达到防止创面加深、促进创面愈合的作用。     

1.5T MRI静磁场对小鼠烫伤创面修复作用初探

目的　探讨1.5T MRI 磁疗对小鼠烫伤创面修复的影响。方法　取健康雄性和雌性小鼠各14只,将砝码于沸水中加热10min后置于经脱毛处理的小鼠背部皮肤,建立深Ⅱ度烫伤模型（病理切片证实）。成模后雄性小鼠和雌性小鼠均随机分为2组,即实验组和对照组,每组7只：(1)实验组小鼠创面在涂抹凡士林的同时接受磁疗,磁场强度为1.5T,每天治疗1次,每次30min,共治疗12d;(2)对照组小鼠创面单纯外涂凡士林治疗。分别于磁疗前（第0天）及磁疗后第3、6、9、12天观察两组小鼠创面修复情况。结果　实验组和对照组小鼠创面面积在各观察时间点均无明显统计学差异,病理结果亦无明显组织学差异;而磁疗第3、6、9 天,雄性和雌性实验组小鼠创面恢复情况均存在统计学差异。结论　1.5T MRI磁疗对小鼠烫伤创面的修复作用不显著。     

A prospective randomised clinical pilot study to compare the effectiveness of Biobrane® synthetic wound dressing, with or without autologous cell suspension, to the local standard treatment regimen in paediatric scald injuries

Background

Scald is the most common cause of burn in children in Australia. The time taken by the burn wound to heal impacts on scar outcome. Commonly scald injuries are treated conservatively; in our unit the practice is that if healing does not occur within 10 days, surgery is used to aid healing with the aim of improving scar outcome. This randomised controlled pilot study compares early treatment regimens to facilitate tissue salvage and reduce the incidence of definitive surgery at 10 days following scald injury.

Methods

All paediatric patients with partial thickness scald injury were clinically assessed between July 1, 2009 and June 30, 2010. A burn of 2% TBSAB or more and deemed not to heal within 10 days, were considered for the trial. These patients were randomised to one of three treatment arms: the local standard treatment (Intrasite™, Acticoat™ and Duoderm^® dressings every 2–3 days) with surgery at 10 days, Biobrane^® only or Biobrane^® and autologous cell suspension using the ReCell^® kit. The primary outcome was surgery performed after 10 days; secondary outcomes were rates of healing, pain experienced, and scar outcomes.

Results

15% of scald presentations in the 12 month period met the eligibility criteria. 13 patients were recruited into the pilot study; early intervention was associated with a decreased time to healing with fewer dressing changes, less pain and better scar outcomes.

Conclusion

Investment of surgical resources in the acute stages within 4 days of injury saved on nursing time, dressing, analgesic and scar management costs.     

复方紫草油的烫伤治疗作用及其机制研究

目的通过复方紫草油对烧烫伤大鼠治疗作用机制的研究,为临床针对性的治疗及护理提供基础资料。方法将60只SD大鼠随机分为5组,各20只,正常对照组、单纯脱毛组、复方紫草油治疗组、京万红烫伤膏治疗组、自然恢复组。采用酶联免疫法测定血浆中脂质氧化、一氧化氮的含量变化。结果复方紫草油治疗组、京万红烫伤膏治疗组、自然恢复组脂质氧化含量都明显升高(P<0.05);京万红烫伤膏治疗组、自然恢复组一氧化氮含量在烫伤后2、4 h逐渐增加,8 h达高峰,24 h后降低,差异有统计学意义(P<0.05);复方紫草油治疗组一氧化氮含量在烫伤前后波动较小;复方紫草油治疗组愈合时间少于京万红烫伤膏治疗组和自然恢复组,差异有统计学意义(P<0.05)。结论紫草油擦剂具有促进组织再生、抗菌消炎、止痛、抑制渗出等功效,能够参与组织的修复,加快组织的再生和愈合速度。     

自制复方虎榆酊治疗烫伤的临床体会

目的：总结应用复方虎榆酊的临床经验。方法：对我院急诊门诊2009—2014年确诊为Ⅰ度及浅Ⅱ度烫伤患者64例的治疗效果进行分析,治愈率100%,创面愈合时间最短5d,最长14d。结果：复方虎榆酊具有止痛效果好,抗感染能力强,清除余热快,创面结痂快,减少瘢痕形成等功效。结论：复方虎榆酊配制方法简单,使用方便,容易掌握,效果满意,值得临床推广。     

浅析哈萨克医学肝系理论与治疗烧烫伤常用药物的关联性

通过对哈萨克医学治疗烧烫伤药物与肝系理论关联性进行研究,分析与总结治烧烫伤的常用药物,为烧烫伤的传统药物治疗提供有益探索。     

大鼠烫伤后血浆NPY与DAO含量变化的研究

目的：探讨大鼠烫伤后血浆NPY与DA0变化及其意义。方法：分别采用放射免疫分析法、分光光度法测定烫伤后不同时间点血浆NPY含量和DA活性。结果：大鼠烫伤后血浆NPY逐步升高，12小时组最高，24小时组基本恢复正常；烫伤后血浆DAo活性逐步升高，伤后12小时组最高，但24小时组乃明显高于对照组；烫伤后血浆NPY与DAo的变化呈正相关。结论：大鼠烫伤后血浆NPY和DAO均升高且呈正相关，NPY升高可能是造成肠缺血缺氧的因素之一。