穹隆-海马伞切断对大鼠脑内TrkA表达的影响

①目的　探讨穹隆 海马伞切断对大鼠脑内不同部位神经生长因子受体TrkA表达的影响及临床意义。②方法　成年健康雌性Wistar大鼠 10只 ,随机分为穹隆 海马伞切断模型组和假手术组。两组大鼠均常规取海马CA1区、皮质区、杏仁复合体区、基底前脑Meynert核等部位脑组织与假手术组比较TrkA阳性细胞表达情况。③结果　假手术组大脑各观察区有基础水平的TrkA表达。模型组大脑海马CA1区、大脑皮质区、杏仁复合体区以及Meynert核区TrkA阳性细胞数明显减少 (t=3.94 4～ 8.4 4 2 ,P <0 .0 5 )。 ④结论　穹隆 海马伞切断可致大鼠脑内多部位TrkA表达减少 ,其可能是导致认知和情绪损伤的原因之一。     

The effect of the fiber components cellulose and lignin on experimental colon neoplasia

Sixty Sprague-Dawley rats were pair-fed one of three nutritionally identical diets. One diet contained “low-fiber” (3.8% crude fiber); the others contained “high fiber” (28.7% crude fiber) composed of either cellulose or lignin. Although both “high fiber” diets had similar stool bulking effects, only the cellulose diet was associated with a reduction in 1,2-dimethylhydrazine (DMH)-induced colon neoplasms. The cellulose diet was also associated with distinct changes in the gut bacterial profile and with a lowered serum cholesterol. © 1993 Wiley-Liss, Inc.     

Roles of DNA polymerase β on repair of DNA damaged by γ－rays irradiation

ＲｏｌｅｓｏｆＤＮＡｐｏｌｙｍｅｒａｓｅβｏｎｒｅｐａｉｒｏｆＤＮＡｄａｍａｇｅｄｂｙγ－ｒａｙｓｉｒｒａｄｉａｔｉｏｎＣａｉＪｉａｎｍｉｎｇ（蔡建明）；ＺｈｅｎｇＸｉｕｌｏｎｇ（郑秀龙）；ＬｕｏＣｈｅｎｇｊｉ（罗成基）；ＧａｏＪｉａｎｇｕｏ（高建国...     

大鼠正中隆起及垂体结节区生长抑素免疫阳性成分的分布

应用免疫组织化学方法观察了大鼠正中隆起及垂体结节区内生长抑素免疫阳性成分的分布,新发现:在交叉后区的正中隆起前部内侧带,有少量的生长抑素免疫阳性神经元胞体;少量阳性纤维几乎垂直行向腹侧,有的可达脑膜表面。垂体结节区内可见较密集的生长抑素阳性纤维丛,其上可见较多的膨体;一些纤维同样到达腹侧表面。     

热休克反应对大鼠油酸肺保护作用的机制研究

未研究发现，经热休克处理的大鼠注油酸后，肺组织损伤明显减轻，Ｗｅｓｔｅｒｎ印迹分析显示热休克使大鼠肺组织中ＨＳＰ７０含量明显增多；Ｎｏｒｔｈｅｒｎ印迹分析证实此乃由于ＨＳＰ７０ｍＲＮＡ转录增加所致。说明热休克处理使肺组织ＨＳＰ７０基因表达增强；提示热休克反应对大鼠油酸肺的保护作用与肺组织细胞中ＨＳＰ７０基因表达增强及与ＨＳＰ７０增多有关。     

我国正常中老年人心率变异分析

心率变异分析（ＨｅａｒｔｒａｔｅｖａｒｉａｂｉｌｉｔｙＨＲＶ）目前被认为是一项预测心性猝死，特别是预测心肌梗死后病人猝死危险性的独立、敏感的指示。目前尚缺乏大样本ＨＲＶ时相分析正常值范围。本研究采用英国ＯＸＦＯＲＤ公司ＭｅｄｉｌｏｇＥＸＣＥＬ长程心电图分析系统，分别用标准差法（Ｓｔａｎｄａｒｄｄｅｖｉａｔｉｏｎ，ＳＤ）和ＨＲＶ指数法（ＨＲＶＩｎｄｅｘ）对１２０名（男：女＝９７：２３）４０一７０岁（平均５７岁，＜６０岁者６６人，≥６０岁者５４人）健康人的２４小时ＨＲＶ值进行测算。其结果为，ＳＤ：１１８．１５±１５．３７ｍｓ．ＨＲＶＩｎｄｅｘ１８．６２±２．３１；中年组（＜６０岁）平均ＳＤ：；１２２．０３ｍｓ．平均ＨＲＶＩｎｄｅｘ：２０．１６；老年组（≥６０岁）平均ＳＤ：１０７。９２ｍｓ．平均ＨＲＶＩｎｄｅｘ：１６．４１。两种方法的结果均显示。ＨＲＶ日间低于夜间，中年人高于老年人，３小时９时ＨＲＶ最低，与晨间冠心病急性发作事件高峰相吻合。本文并对ＨＲＶ的昼夜变化规律及其意义进行了初步探讨。     

脉冲Nd：YAG激光照射对大鼠炎症牙龈组织的作用

患实验性牙龈炎的大鼠牙龈（６例）经脉冲掺钕钇铝柘榴石激光（Ｎｄ：ＹＡＧ激光）照射后进行光镜及超微结构观察。目的在于了解低能量Ｎｄ：ＹＡＧ激光照射对牙龈炎症的治疗作用。炎症牙龈经输出能量为１．５Ｗ，脉冲数为２０ＰＰＳ的激光照射后与对照组相比较，形态学上有如下变化：上皮细胞无变性；牙龈上皮层数较炎症时减少；上皮下炎细胞浸润明显减少；上皮细胞间隙变小；上皮下结缔组织内产生大量的排列致密的胶原纤维。结果提     

Differences in the hypothermic response to ethanol in rats selectively bred for oral ethanol preference and nonpreference

The duration of retention of tolerance to ethanol was tested in the alcohol-preferring (P) and alcohol-nonpreferring (NP) rats lines, using ethanol-induced hypothermia as a measure of tolerance. Rats received two injections of ethanol (3.5 g/kg) body wt, IP) and the time between the injections was 1, 2, or 3 days. When one day separated the two injections, tolerance to the hypothermic effect of a second “test” injection was found in both lines. When 2 or 3 days separated the two injections, the P line showed a loss of tolerance and the NP line showed sensitization to ethanol. Sensitization in the NP line grew stronger when the interval between injections was increased from 2 to 3 days. The duration of retention of tolerance to ethanol-induced hypothermia in the P line was shorter than has previously been reported for motor impairment in this line. It appears that the duration of tolerance retention in the P line depends on the test used to measure tolerance. Sensitization to ethanol in the NP line may be associated with low oral ethanol intake. This research was supported, in part, by grants AA08312, AA03243, and AA07611 from the PHS     

实验性颅脑伤大鼠脑组织SOD和MDA的变化

目的：观察实验性颅脑伤大鼠脑组织超氧化物歧化酶(SOD)和丙二醛(MDA)的动态变化，探讨SOD和MDA在颅脑伤病理损伤机制中的作用。方法：采用自由落体致伤方法制备大鼠颅脑伤模型(模型组)，并以正常大鼠作对照(对照组)，分别于造模后3、5和7d处死动物取脑，测定海马和皮质中SOD活力和MDA含量。结果：模型组大鼠海马SOD活力在3个时间点均显著低于对照组，而MDA含量均显著高于对照组；模型组大鼠皮质SOD活力在造模后3、5d时显著低于对照组，而MDA含量则显著高于对照组，到7d时二者与对照组均无明显差异。结论：大鼠颅脑伤时脑组织SOD活力显著降低，而MDA含量则显著增高，提示SOD和MDA可能参与颅脑伤病理损伤机制并在其中发挥重要作用。