脉血康胶囊对脑梗死恢复期患者疗效及对凝血功能的影响

目的:观察脉血康胶囊对脑梗死恢复期患者临床疗效及其对凝血功能影响.方法:将120例恢复期脑梗死患者随机分为治疗组和对照组.对照组50例采用常规治疗,治疗组70例在常规治疗基础上加用脉血康胶囊治疗3个月,治疗前后分别测定神经功能缺损评分及凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fib)水平.结果:治疗后治疗组PT,APTT,TT较治疗前时间延长(P＜0.05),Fib、神经功能缺损评分下降(P＜0.01),且与对照组治疗后比较差异明显(P＜0.05).结论:脉血康胶囊是治疗脑梗死恢复期患者安全有效的药物之一,能改善患者的血凝状态.     

高效液相色谱梯度洗脱法同时测定复方间苯二酚乳膏中两组分含量

 目的 建立高效液相色谱梯度洗脱法同时测定复方间苯二酚乳膏中间苯二酚和醋酸曲安奈德含量的方法。方法 使用ZORBAX Eclipse XDB-C₁₈（4.6 mm×250 mm,5 μm）,流动相为乙腈（A）-0.5%醋酸铵溶液（B）,进行梯度洗脱,0～3 min (25% A),3～5 min (25% A～55% A),5～15 min (55% A),柱温40 ℃,流速1.0 mL·min^-1,检测波长240 nm。结果 间苯二酚和醋酸曲安奈德的线性范围分别为： 103.18~515.90 μg·mL^-1(r=1.000 0),1.336~6.680 μg·mL^-1(r=0.999 4);平均回收率分别为99.3%,99.1%;RSD分别为0.62%,0.73%。结论 该方法简便,快速,准确,用于实际样品的测定,结果满意。     

An endogastric capsule for measuring tumor markers in gastric juice: an evaluation of the safety and efficacy of a new diagnostic tool.

BACKGROUND: In gastric juice, high levels of the carcinoembryonic antigen (CEA) and the carbohydrate antigen 19-9 (CA 19-9) have been found to correlate with precancerous lesions and gastric cancer. So far, sampling of gastric juice has required upper endoscopy. In place of this invasive procedure, we investigated a new tool for the quantitation of tumor markers in gastric juice. MATERIALS AND METHODS: The study population consisted of healthy controls and consecutive subjects with suspected gastric cancer or dyspepsia/epigastric distress. Patients were asked to swallow a small gelatine capsule (14 mm in length and 5 mm in diameter) containing a pierced plastic cover and surrounding a piece of absorbent paper. The capsule was left in the gastric cavity for 60 min to allow saturation of the absorbent paper with gastric juice. A 45-50 cm length of nylon thread connected to the inner capsule was used to remove the device from the gastric cavity. After processing the absorbent paper for radioimmunoassay, CEA and CA 19-9 levels were correlated to the findings of upper endoscopy and biopsies of gastric mucosa or suspected lesions. RESULTS: The endogastric capsule did not cause any side-effects and 62 participants were fully compliant to the procedure. Assessable gastric juice samples were taken from 23 patients with gastric cancer, 15 patients with intestinal metaplasia or dysplasia, 12 patients with gastritis and 12 controls without gastric diseases. In the 12 samples of gastric juice from control patients, mean values of CEA and CA 19-9 were 1.1 +/- 0.9 ng/ml and 16 +/- 7.5 ng/ml, respectively. The mean levels of both markers were found to increase according to the severity of gastric lesions and in patients with cancer, mean CEA and CA 19-9 levels were 513 +/- 627 ng/ml and 545 +/- 510 ng/ml, respectively. Patients with precancerous lesions and cancer showed higher levels of CEA and CA 19-9 than patients with normal findings or gastritis (P <0.001). CONCLUSIONS: The endogastric capsule is a simple, non-invasive tool for the measurement of CEA and CA 19-9 levels in gastric juice. These values may discriminate between normal or minor pathologic changes and precancerous lesions or carcinomas. Further investigations are warranted, since this may represent a new method for gastric cancer screening.     

哺乳期桡骨茎突狭窄性腱鞘炎18例分析

治疗哺乳期桡骨茎突狭窄性腱鞘炎采用热敷法,用双虚肿痛宁搽剂外涂,曲胺奈德注射液封闭,三七血伤宁胶囊口服,共18例,1次愈者15例,2次愈2例,3次愈1例.     

Partial characterization of the fibroblast chemotactic constituents of human aqueous humour

This study demonstrates that aqueous humour derived from patients undergoing cataract extraction contains chemoattractants for ocular fibroblasts. The chemoattractant activity is independent of the surgical approach to obtaining the specimen as well as the surgeon operating. Studies in rabbits demonstate that the chemoatractant activity is not artefactually produced by aqueous sampling and confirm the integrity of the blood-aqueous barrier. Substances resonsible for this activity have molecular weights greater than 30,000; they are deactivated by low pH but retain activity at high pH. Identification of the chemoattractants in aqueous humour may allow pharmacological manipulation of would healing in relation to trabeculectomies.     

反相高效液相色谱法测定中黄胶囊中葛根素的含量

目的 :建立中黄胶囊中葛根素的含量测定方法 ,以控制该产品的质量。方法 :采用反相高效液相色谱法 ,以DiamonsilC18 为固定相的色谱柱 ,甲醇 -0 015 %磷酸溶液 (23∶77)为流动相 ,检测波长249nm。结果 :葛根素的进样量在0 020μg～0 120μg 范围内与其峰面积有良好的线性关系 (r=0 9999) ,平均加样回收率为98 54 % ,RSD为1 07 %。结论 :该方法简便易行、准确可靠 ,可用于中黄胶囊的质量控制     

口溃宁胶囊在动物体内的药理作用

目的：观察口溃宁胶囊的抗炎作用及提高机体免疫力的作用。方法：以二甲苯所制的炎症试验，以贞芪扶正冲剂阳性对照的免疫试验。结果：口溃宁胶囊高剂量对二甲苯所致小鼠耳肿胀均有明显抑制作用及对小鼠体液免疫有明显促进作用。结论：口溃宁胶囊具有抗炎及提高机体免疫力的作用。     

Prediction of individual tumor chemosensitivity in subrenal capsule assay

Fifty-three tumor specimens, including thirty-one stomach and seven esophageal cancers, were examined to determine the individual tumor sensitivity to chemotherapeutic agents. Using the subrenal capsule assay (SRC assay), tumor specimens were implanted under the renal capsule of male, 8 week old ddY mice, after cutting the tissues into 1.5 mm cubed fragments. Following the implantations, chemotherapeutic agents were injected daily for 3 days and the relative variations of tumor weights were calculated. An 84.9 per cent of the total evaluability rate was obtained and implanted tumor specimens responsed to chemotherapeutic agents in 26.7 per cent. The correlation rate between tumor sensitivity in SRC assay and clinical responses was obtained in 72.9 per cent. The predictive accuracy rate of the clinical responses was 50.0 per cent, while 100 per cent of the prediction rate of clinical resistance was obtained. With regard to upper gastro-intestinal cancers, 83.9 per cent of the evaluability rate and 18.2 per cent of response rate in SRC assay were obtained. These results indicate that this assay is equivalent to other procedures for predicting individual tumor sensitivity.     

Untersuchungen zur elektronenmikroskopischen Darstellung der Kapsel von Cryptococcus neoformans. Ultrastructural Research on the Capsule of Cryptococcus neoformans

Zusammenfassung: An drei Cryptococcus-neoformans-Stämmen wurden elektronenmikroskopische Untersuchungen zur Darstellung ihrer Kapsel durchgeführt. Mittels polykationischem Ferritin gelang die kontrastreiche Markierung der Polysaccharidkapsel auf der Basis einer zytochemisch-physikalischen Reaktion zwischen den negativen Ladungsträgern der Extrazellularschicht und dem kationischen Marker. Zusätzlich konnten extrazellulär fibrilläre Strukturen differenziert werden, an die eine Anlagerung von Ferritin nicht erfolgt war. Geringfügige methodische Variationen verringerten die Ferritinmarkierung erheblich, eine einstündige Hitzebehandlung hob sie ganz auf. Dagegen traten unmarkierte Bestandteile verstärkt in den Vordergrund. Bei dem Versuch einer Interpretation dieser elektronenoptischen Befunde wurde die Möglichkeit diskutiert, daß die Kapsel von Cryptococcus neoformans einen von dem leicht ablösbaren Polysaccharid zu unterscheidenden Anteil mit fibrillärem Aufbau enthält, der evtl. im Sinne eines Stützgerüstes für die Ein- und Anlagerung des löslichen Kapsel-Antigens fungiert. Summary: Ultrastructural research on the capsule of Cryptococcus neoformans. For ultrastructural visualization of their capsule, three Cryptococcus neoformans-strains were treated cytochemically with a polycationized derivative of ferritin. This marker binds electrostatically to the anionic sites of polysaccharide capsules. After treatment, the yeast cells were covered with a dense irregular layer of ferritin granules. Moreover, fibrillary components could be detected extracellularly, which did not bind cationic ferritin. Slight methodical variations, e. g. pretreatment of the yeasts by heat, led to an intensification of these structures, whereas binding of ferritin decreased or was abolished, respectively. These findings indicate that the capsule of Cryptococcus neoformans apparently consists of two different components: one seems to bind cationic ferritin, is easily removable, and equivalent to the soluble polysaccharide antigen found in serum and liquor during Cryptococcus-infection, and a second fibrillary one is possibly acting as a supporting framework for the soluble component.