三种中草药制剂对肠道致病菌体外抗菌作用的分析

目的：探讨分析三种中草药制剂对肠道致病菌体外抗菌作用。方法以连续稀释法检测三种中草药制剂（黄柏、黄连、黄芩）的最低抑菌浓度。结果三种中草药制剂对大肠杆菌的最低抑菌浓度：黄柏为6.3 g/L,黄连为1.6 g/L,黄芩为6.3 g/L;对金黄色葡萄球菌的最低抑菌浓度：黄柏为3.2 g/L,黄连为0.8 g/L,黄芩为1.6 g/L;对副溶血性弧菌的最低抑菌浓度：黄柏为0.8 g/L,黄连为0.8 g/L,黄芩为1.6 g/L;对沙门菌的最低抑菌浓度：黄柏为3.2 g/L,黄连为3.2 g/L,黄芩为12.5 g/L;对志贺菌的最低抑菌浓度：均为3.2 g/L。结论三种中草药制剂（黄柏、黄连、黄芩）对肠道致病菌体外抗菌作用较强。     

数字化色谱指纹谱法控制半夏的质量

目的:建立半夏的数字化色谱指纹谱,以对其进行质量控制。方法:采用HPLC梯度洗脱技术进行分离分析,色谱柱:AgilentZorbaxODSC18柱;流动相:乙腈-水作梯度洗脱;检测波长:210nm;流速:0.5mL/min;柱温:25℃。结果:在选定的色谱条件下,建立了半夏的数字化色谱指纹谱,制定了控制半夏质量的指纹谱技术参数。结论:利用数字化色谱指纹谱技术控制半夏的质量是可行的。     

黔曲质量标准

目的:建立黔曲质量标准。方法:用薄层鉴别方法鉴别木香、大黄、枳实,用HPLC测定橙皮苷含量,色谱柱为Dikma Diamonsil C18(4.0 mm×250 mm,5μm),流动相乙腈-0.02%磷酸水溶液(19∶81),流速1.0 mL.min-1,检测波长283 nm。结果:定性鉴别分离度好、重复性好、专属性强;橙皮苷线性范围为10.568～528.4 mg.L-1(r=0.999 98),平均回收率为100.18%,RSD 2.73%,精密度RSD 0.37%(n=6),重复性RSD 0.88%(n=6)。结论:方法可靠、准确、专属性强,可用于该制剂的质量控制。     

大黄清胃冲剂活性成分提取工艺的均匀设计优化选择

目的 探讨大黄清胃冲剂活性成分提取分离的优化工艺条件。方法 以游离蒽醌提取率为定量指标,通过４种生长黄煎煮方法的初步筛选,确定浸渍溶剂、浸渍时间、回流溶剂及回流时间为考察因素,并为Ｕ９（９＾６）均匀表设计试验。结果 大黄提取优化工艺条件为７０％乙醇浸润１４ｈ和回流提取８０ｍｉｎ。结论 工艺条件可以用于大黄清胃冲剂的生产制备。     

骨宝对正常大鼠骨组织形态学影响的定量研究

分别用５、１５ｇ／ｋｇ的骨宝给３月龄ＳＤ大鼠灌胃、每周６次，１８０ｄ。不脱钙骨制片，用图象定量法，测量大鼠胫骨近有小梁。结果：高剂量骨宝组与对照组比较，骨吸收减少３１％，骨面积增中３９％。     

大黄地上部分化学成分与药理作用研究进展

大黄为传统大宗中药材,临床应用广泛,国内外需求量极大,但其传统药用部位为根及根茎,而占大黄总生物资源二分之一以上的地上部分作为非药用部位被丢弃,浪费资源的同时造成一定的环境压力。研究表明,大黄地上部分富含蛋白质、粗纤维、果胶、氨基酸等营养成分,以及蒽醌类、黄酮类、二苯乙烯类等化合物,具有泻下、抗炎、止血、抗氧化、降血糖、降血脂等药理活性。在查阅国内外文献的基础上,本文对大黄地上部分的化学成分和药理作用进行综述,并对其开发前景进行展望,为其深入研究和综合开发利用提供参考。     

Effects of traditional herbal medicine, Hwaotang, on atherosclerosis using the spontaneous familial hypercholesterolemia model, Kurosawa and Kusanagi-hypercholesterolemic rabbits and the venous thrombosis rats

Hwaotang (HOT), a traditional Korean medicinal formulation, is a dried decoctum of a mixture of seven herbal medicines, consisting of Angelica gigantis Radix, Rehmanniae Radix, Paeoniae Radix, Ciniamomi Cortex, Cnidii Rhizoma, Persicae Semen and Carthami Flos. In the present study, the inhibitory effects and anti thrombic properties of HOT on the progression of atherosclerotic lesions were studied using the spontaneous familial hypercholesterolemia (FH) model, Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbits and rats. Changes in blood chemistry, pathology and low-density lipoprotein (LDL) oxidation were measured in a control and HOT group. In the control group, the area of atheromatous plaques of the aorta progressed between week 12 (36.65%) and week 14 (46.22%). This progression of atherosclerotic lesions did not occur in the HOT-treated group after 12 (24.24%) and 14 (23.34%) weeks. Antioxidative effects on LDL were seen in the HOT in weeks 12 and 14. HOT improved the hypercholesterolemia in the KHC rabbits. On the other hand, HOT and five of the seven herbs, except Cnidii Rhizoma and Carthami Flos, inhibited the endotoxin-induced hepatic venous thrombosis in high cholesterol diet-treated rats. However, Ciniamomi Cortex showed a very weak inhibitory effect on the endotoxin-induced hepatic venous thrombosis. The extract also inhibited the endotoxin-induced decrease in blood platelets and fibrinogen, and endotoxin-induced increase in fibrin degradation products (FDP) on disseminated intravascular coagulation in normal rats. In conclusion, these results suggest that HOT has inhibitory effects on the development of atheromatous plaque formation in spontaneous FH rabbits. It is also suggested that the antioxidative effects of HOT on LDL led to the beneficial effects observed in this study. The protection by HOT and its herbs on the artificially induced ischemic infarction might be related to their inhibitory effects on disseminated intravascular coagulation, platelet coagulation and thrombotic action.     

甘草炭炮制工艺及质量标准研究

目的：优选甘草炭的炮制工艺并制定其质量标准。方法：以浸出物和甘草苷、甘草酸含量为评价指标,炒炭温度、炒炭时间、翻动频率为考察因素,采用正交试验设计优选甘草炭炮制方法,对其性状、显微鉴别、薄层色谱、总灰分、浸出物进行测定,采用高效液相色谱法（HPLC）对甘草炭中的甘草苷、甘草酸进行定量分析。结果：甘草炭的最佳炮制工艺为炒炭温度360℃,炒炭时间6 min,翻动频率20 r·min–1;甘草炭总灰分为5.2%、浸出物为18.5%、甘草苷质量分数为0.066%、甘草酸质量分数为0.24%。结论：建立了甘草炭的炮制工艺及质量标准,可为甘草炭饮片生产及质量控制提供参考。     

气滞胃痛颗粒质量标准研究

目的建立气滞胃痛颗粒的质量控制方法。方法采用薄层色谱(TLC)法对气滞胃痛颗粒中的香附、枳壳和甘草进行定性鉴别。采用高效液相色谱(HPLC)法对气滞胃痛颗粒中的延胡索乙素进行含量测定,色谱柱采用Alltima C18柱(250 mm×4.6 mm,5μm),以0.1%磷酸溶液(用三乙胺调pH至6.0)-乙腈(59∶41)为流动相,流速为1.0 mL/min,检测波长为280 nm,柱温为30℃。结果枳壳、香附和甘草薄层色谱法鉴别专属性强,延胡索乙素进样量在0.050 1～1.002μg范围内与峰面积呈良好线性关系(r=0.999 9,n=7),延胡索乙素平均回收率为99.94%,RSD=1.60%(n=6)。结论所用方法可准确地定性、定量,重现性好,可用于控制气滞胃痛颗粒的质量。     

大黄紫草巴布剂基质处方研究

目的筛选大黄紫草巴布剂的基质处方。方法采用均匀设计方法,以初黏力、持黏力为评价指标,各基质及其用量为因素和水平进行均匀设计实验,采用SPSS16．0进行方程拟合,分析各因素对基质性能的影响并得出最优配比组成。结果巴布剂最佳配比为NP-700-氢氧化铝-甘油0．2％酒石酸溶液-4．0：0．2：20：10。结论该基质具有良好的外观和黏附性能,与大黄紫草提取物有良好的相容性。