MODIFICATION OF THE LOWRY METHOD FOR ANALYSIS OF SOLUBLE LATEX PROTEINS

Proteins from natura lrubber latex (NRL) can cause local and systemic reactions in people who are allergic to it. Reducing the total protein in NRL products to minimize the allergenic potential requires methods of accurately estimating the amount of protein. Previous work in this laboratory and others has indicated that, of the colorimetric assays, a modified Lowry method performs best. The American Society for Testing Materials (ASTM) has adopted the modified Lowry method for analysis of soluble NRL proteins.This method, although the best currently available, lacks sufficient reproducibility and sensitivity. In anattempt to improve the method, we address in this article the importance of various extraction conditions and additional approaches to eliminate interfering substances that may cause the false-positive values in the test. Depending on the chemical composition of the accelerants added during manufacturing processes, different means may be employed to remove or to account for the presence of these small organic molecules. The best general way to remove these chemical compounds is the partitioning of the latex extracts with ethyl acetate followed by acid precipitation. Although there is no one perfect method for all NRL proteins and products, the data presented here indicate that changes in the standard protocol of the Lowry method may result in more reproducible and reliable results.     

尿沉渣定量分析方法的评价及影响因素研究

目的　探讨尿沉渣定量分析方法及其影响因素。方法　选择经肾活检确诊的肾脏病患者以及肾移植患者共 10例 ,比较显微镜尿沉渣镜检染色定量计数板和尿沉渣自动定量测定仪 (UF 10 0 )对同一样本中管型和上皮细胞的检测结果 ;细胞染色采用改良巴氏染色 ;尿液细胞碎片采用流式碘化丙啶 (PI)染色检测。结果　流式细胞仪检测尿沉渣中的管型和上皮细胞较显微镜镜检结果分别增高 8.83倍和 5 .83倍 (P <0 .0 5 )。对患者同一尿沉渣分别用HDF液、正常人尿液和患者尿液进行悬浮并置 4℃保存 ,随着留置时间的延长 ,其上皮细胞数均逐渐减少 (P <0 .0 5 ) ,同时均伴有细胞碎片率逐渐增加。在 8h和 16h ,用患者尿液处置的尿沉渣细胞碎片率均明显高于HDF组 (P <0 .0 5 )。在 8h时 ,用正常人尿液处置的尿沉渣细胞碎片率明显低于患者尿液处置组(P <0 .0 5 )。而各组患者尿沉渣管型计数的差异无显著性 (P >0 .0 5 )。患者尿沉渣 (3例 )分别用HDF、丙酮、乙醇和多聚甲醛固定、置 - 2 0℃保存 1周后进行PI染色 ,其细胞碎片率分别为 (31.6± 6 .4 ) %、(6 6 .4± 2 .7) %、(4 9.6± 1.4 5 ) %和 (6 5 .9± 3.4 ) % ,HDF和乙醇组细胞碎片率低于丙酮和多聚甲醛组 (P <0 .0 5 )。结论　应用荧光流式细胞仪和显微镜镜检定量计数对     

肝纤维化的影像学定量研究进展

肝纤维化是肝组织对慢性损伤的修复反应,其特征是持续慢性炎症及胶原纤维沉积。影像学作为无创性手段,可以对肝纤维化进行全面、重复且定量的进行评估。本文综述了近年来开展的影像学定量评价肝纤维化的方法,为肝纤维化影像学定量研究提供依据。     

小细胞肺癌患者胸水、血清中NSE、 pro-GRP定量分析与免疫组化分析的诊断一致性评价

目的 评价小细胞肺癌（SCLC）患者胸水、血清中NSE、pro-GRP定量分析与免疫组化分析的诊断一致性.方法 采用免疫组化方法对病理标本的NSE、pro-GRP表达进行分析,用化学发光法对血清和胸水NSE、pro-GRP表达水平做定量分析.结果 小细胞肺癌患者血清和胸水NSE、pro-GRP表达水平明显高于非小细胞肺癌（NSCLC）患者.NSE、pro-GRP免疫组化分析与血清和胸水NSE、pro-GRP定量分析诊断阳性率无统计学差异.结论 小细胞肺癌患者手术前缺乏病理组织材料时,可定量分析血清和胸水NSE、pro-GRP表达水平获得与免疫组化分析一致的诊断效果.     

Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation

The performance of a recently developed real-time PCR system, the ExiStation HBV diagnostic system, for quantitation of hepatitis B virus (HBV) in human blood was evaluated. The detection limit, reproducibility, cross-reactivity, and interference were evaluated as measures of analytical performance. For the comparison study, 100 HBV-positive blood samples and 100 HBV-negative samples from Korean Blood Bank Serum were used, and the results of the ExiStation HBV system showed good correlation with those obtained using the Cobas TaqMan (r² = 0.9931) and Abbott real-time PCR systems (r² = 0.9894). The lower limit of detection was measured as 9.55 IU/mL using WHO standards and the dynamic range was linear from 6.68 to 6.68 × 10⁹ IU/mL using cloned plasmids. The within-run coefficient of variation (CV) was 9.4%, 2.1%, and 1.1%, and the total CV was 11.8%, 3.6%, and 1.7% at a concentration of 1.92 log₁₀ IU/mL, 3.88 log₁₀ IU/mL, and 6.84 log₁₀ IU/mL, respectively. No cross-reactivity or interference was detected. The ExiStation HBV diagnostic system showed satisfactory analytical sensitivity, excellent reproducibility, no cross-reactivity, no interference, and high agreement with the Cobas TaqMan and Abbott real-time PCR systems, and is therefore a useful tool for the detection and monitoring of HBV infection.     

微血管计数、CD44V6表达与乳腺癌临床病理因素及预后的关系

目的：探讨乳腺癌组织中微血管计数、ＣＤ４４Ｖ６表达与临床病理因素及预后的关系。方法：对经治疗后随访８年以上的乳腺癌病例，应用免疫组化法，分别检测癌组织中微血管计数及ＣＤ４４Ｖ６表达。将其结果与影响预后的临床病理因素及生存状况比较。结果：肿瘤内微血管数与组织学分级、临床分期、淋巴结转移呈正相关。死亡组癌组织内微血管数明显高于存活组。 ＣＤ４４Ｖ６在癌组织中的表达率为 ８０．４％，淋巴结阳性及组织学分级高的病例 ＣＤ４４Ｖ６阳性表达率增加，死亡组ＣＤ４４Ｖ６表达明显高于生存组。结论：微血管计数及ＣＤ４４Ｖ６可做为估计乳腺癌预后和指导临床治疗有价值的参考指标。     

应用磁共振频谱诊断脑膜瘤的进展

脑膜瘤是颅内脑外常见的脑外肿瘤,典型脑膜瘤影像诊断并不困难,但不典型者与其他颅内脑外肿瘤及部分靠近脑实质表面向脑外生长脑实质肿瘤不易鉴别,如何提高不典型脑膜瘤的诊断是一个亟待解决的问题。近年来,磁共振频谱技术的不断发展及临床经验的积累为不典型脑膜瘤的诊断提供了一种无创地、可重复地、可靠地检测方法。     

肝动注阿霉素及其脂质体在体内的定量分析

进一步研究阿霉素及其脂质体在体内的行为,需要灵敏、精确的定量分析方法,本研究建立了适用于微量阿霉素及其脂质体以及其代谢产物Adriamycinol和Adriamycinone在动物组织、血浆及尿中的高效液相分析方法。     

The correlation between HIF-1 alpha and VEGF in oral squamous cell carcinomas: Expression patterns and quantitative immunohistochemical analysis

Background

The prognostic predict biomarkers are important in oral squamous cell carcinoma (OSCC). We investigated the expression patterns and quantitation of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in OSCC patients. Among OSCC patients with recurrence and metastasis, the expression percentages of HIF-1α and VEGF also were analyzed.