李仲愚杵针刺激大鼠"足三里”穴区对中枢单胺类神经递质含量的影响

为探讨李仲愚杵针针刺镇痛效应的中枢作用机制,了橇针对正常大鼠及腹腔注射对氯苯丙氨酸大鼠的不同脑区单胺类递质水平的影响,并与是针作比较。结果显示：杵与电针一样,在刺激大鼠“足三里”穴区后,能明显提高间脑和端脑５－羟色胺与５－羟醋酸吲哚的含量,同时也可使间脑去甲肾上腺素含量明显下降,杵针对端脑的ＮＥ以及两脑区多巴胺的影响未见明显的影响。腹腔注射ＰＣＰＡ后大,大鼠脑内Ｔ－ＨＴ、５－ＨＩＡＡ含量均明显下降     

Toxin pharmacology of the large-conductance Ca2+-activated K+ channel in the apical membrane of rabbit proximal convoluted tubule in primary culture

The patch-clamp technique was used to study the toxin pharmacology of the large-conductance Ca²⁺-activated K⁺ channel (BKCa) present in the apical membrane of rabbit proximal convoluted tubules (PCT) in primary culture. Experiments were performed with the inside-out configuration. This channel was very selective for K⁺ against Na⁺ and had a conductance of 180 pS with 140 mmol/l in the pipette and the bath. The action of toxins was studied on the extracellular side of the channel by using the pipette perfusion technique. Experimental conditions were 140 mmol/l KCl in the pipette and 140 mmol/l Nad in the bath. Pipette potential was maintained at 0 mV. Perfusion of crude venom from Leiurus quinquestriatus hebraeus inhibited reversibly the open probability (P _o) in a concentration-dependent fashion (IC₅₀=0.8 mg/l; n=3). The following synthetic or purified toxins were tested: synthetic charybdotoxin (ChTX) IC₅₀=7.3×10^–9 M (n=5); iberiotoxin (IbTX) IC₅₀=5.5×10^–7 mol/l (n=3); and kaliotoxin (KTX) IC₅₀=4.8×10^–7 mol/l (n=3). The suppression of the six first N-terminal amino-acids slightly reduced the affinity of ChTX (IC₅₀=1.2×10^–8 mol/l, n=4). Neither Dendroaspis polylepis venom nor purified dendrotoxin modified P _o even at high concentrations (20 mg/l and 10^–6 mol/l respectively). Apamin, which blocked the small-conductance K⁺ channel in cultured PCT, did not act on BKCa. These results indicate that ChTX is the most efficient known toxin against the epithelial BKCa in primary cultures of PCT. In spite of there being considerable homology of sequence between ChTX, IbTX and KTX, ChTX was about 100 times more effective than the others. Truncated ChTX kept a high affinity for this channel and could be used to obtain a labelled probe.     

Potassium-selective channels in the basolateral membrane of single proximal tubule cells of frog kidney

The membrane potential of proximal tubule cells is dominated by the potassium conductance of the basolateral membrane. In the present paper the nature of this conductance is investigated by the patch-clamp technique. Only one type of K channel was found in the basolateral membranes of freshly isolated proximal cells. In cell-attached patches, the current/voltage relationship is markedly non-linear with much larger inward (30 pS) than outward ( 6 pS) conductances, even in the presence of roughly symmetrical K concentrations. Thus the channels show inward rectification. The determination of the conductance for outward current flow is complicated since the current/voltage curves show an area of negative conductance. Nevertheless, taking the conductance for outward current flow and the density of the channels it is possible to account for all of the previously reported potassium conductance of amphibian proximal tubule cells. The open probability of the channels was found not to depend upon the membrane potential. However, the non-linearity of the current/voltage relationships will confer upon the channel the same voltage dependence as that seen in intact proximal tubules, i.e. the conductance decreases with depolarisation. Incubation of cells in Ringer with no substrates or in the presence of alanine and/or glucose showed no change in the activity of the channels. These findings suggest that, although these channels may represent the basolateral conductance of frog proximal tubule cells, they are not involved in the well-established coupling between transport rate and potassium conductance.This work was supported by the Wellcome Trust     

三痹汤加味配合蜂针治疗产后痹42例疗效观察

目的：观察三痹汤加味配合蜂针治疗产后痹的临床疗效。方法：选择经活蜂试针为阴性的产后痹患者42例，采用内服三痹汤加味配合蜂针治疗。结果：痊愈34例，有效6例，无效2例，总有效率95．2％。结论：三痹汤加味配合蜂针治疗产后痹能明显改善临床症状，疗效满意，操作简单方便，值得推广应用。     

齐刺、温针灸治疗肱骨外上髁炎74例临床观察

目的：观察针灸治疗肱骨外上髁炎的疗效。方法：126例患者随机分成阿是穴齐刺加温针灸组74例和单一针刺组52例，治疗2个疗程观察比较两组的疗效。结果：齐刺、温针灸组与单一针刺组疗效比较，差异有极显著性意义（P＜0．01），结论：阿是穴齐刺加温针灸治疗该病的临床疗效优于单一针灸疗法。     

同侧锁骨翻转修复肱骨近端肿瘤性骨缺损(附6例报告)

目的 观察同侧锁骨翻转修复肱骨近端肿瘤性骨缺损的临床治疗效果。方法 应用该方法治疗6例肱骨近端骨肿瘤。男2例,女4例,平均年龄28.9岁。骨巨细胞瘤3例,软骨肉瘤、转移癌和骨纤维肉瘤术后复发各1例。结果 所有患者均顺利完成手术。1例失访,5例随访6～40个月。1例死亡,1例移植锁骨骨折,3例骨愈合良好,2例显示锁骨增粗。结论 同侧锁骨翻转是修复肱骨近端肿瘤性骨缺损的一种可行的方法。     

TREATMENT OF 36 CASES OF SCAPULOHUMERAL PERIARTHRITIS MAINLY BY CONTRALATERAL NEEDLING

ScapulohumeralperiarthritisiscalledasLoujianfeng (漏肩风omalgia)orJianning (肩凝frozenshoulder)inTCMandoftenseeninpeopleatageofabout 50 years (thusthereisasayingofWushijianinChinese people) .Itchieflymanifestspainanddysfunctionoftheshoulderjointandisaverycommondiseaseinclinic.Contralateralinsertionoftheacupunctureneedle (contralateralneedling)isanancienttechniqueofacupointassociationandtreatment,whichmeanspuncturingacupointscontralateraltothesideofthebodywiththedisorder.Thisisbecauseofthatw…     

石门穴临床应用举隅

介绍石门穴的局部解剖、针灸方法、主治功效及其对尿路感染、小儿遗尿、妇女避孕等验案四则。     

缪刺考

At present, most physicians hold that Miu Ci is "left and right cross collateral prick-ing",but it does not conform to the original meaning of "Suwen·Miucilunpian"(Plain Questions ·Trea-tise on Miu Ci). Miucilanpian holds that Miu Ci is the extension and perfection of Ju Ci (contralateral me-ridian needling), which mainly pricks the collateral but is not limited to the collateral, as it also pricks the acupuncture point. As for the "left and right cross" point selection, it is one step in the procedure of the treatment in the process of contralateral collateral needling instead of being a principle.     

风门穴与肺俞穴针刺安全深度及角度的应用解剖研究

采用钢针标定法和层次解剖法,在46具成年人尸体上,研究风门穴与肺俞穴的解剖结构和针刺深度及角度。结果显示:向下直刺的解剖结构依次是皮肤、浅筋膜、斜方肌、菱形肌、上后锯肌、竖脊肌、肋提肌、肋间内膜、胸内筋膜、肋胸膜,向下直刺的平均危险深度,风门穴为49.51 mm,肺俞穴为44.88 mm;向外下斜刺,当针体与矢状面夹角在20~25°时,危险深度最小;向内下斜刺,当针体与矢状面夹角大于20°时,比较安全。