超声波提取无患子总皂苷工艺的优选研究

目的:优选超声波提取无患子中总皂苷的最佳工艺。方法:通过正交设计实验,以溶剂体积分数、超声时间、超声功率以及料液比作为影响因素,以总皂苷提取率作为参考指标,优选最佳工艺方案。结果:最佳提取工艺是90%乙醇,料液比为1∶20,90 W功率下超声40 min,其验证实验平均提取率为37.28%。结论:超声提取方法提取率高,结果稳定。     

正交法探讨复方参仲口服液制备工艺

目的:优选复方参仲口服液的制备工艺。方法:采用L9(34)正交试验对制备工艺进行优选。结果:最佳提取工艺为8倍于生药量的水煎煮3次,每次1.0 h,醇沉浓度为70%。结论:该制备工艺合理、可行。     

正交试验法优选金银花总有机酸的提取工艺条件

目的：优选并确立金银花中总有机酸的最佳提取工艺。方法以总有机酸提取率为指标,采用正交设计实验法对提取过程中的影响因素进行优选研究。结果金银花中总有机酸最佳提取工艺为：提取时间1.5 h,提取次数为3次,加水量16倍量。在此条件下,总有机酸提取率达66 mg/g。结论采用此提取方法不仅能够充分提取金银花中总有机酸,而且方法简便,适用于工业化生产。     

Effects of Ethanol Extract of Radix Sophorae Flavescentis on Activity of Colon Cancer HT29 Cells

This paper mainly studied the inhibitory effect of total ethanol extract of Radix Sophorae Flavescentis on proliferation of colon cancer HT29 cells. By reflux extraction method and with ethanol as extraction solvent, different extracts were obtained at different ethanol concentrations, different solid-liquid ratios, and at different times. And their inhibitory activities against HT29 cells were compared using MTT assay. The experimental results showed that the extraction processes under three conditions can all draw relatively high inhibition rates. The optimum ethanol extraction process conditions were as follows: a solid-liquid ratio of 1:9, 80 min of heat reflux extraction with 95% ethanol.     

光固化胶粘剂性能的初步研究

目的：制备一种光固化纳米胶粘剂,对其性能进行初步研究。方法：正交法制备出多个胶粘剂样品,讨论各个因素对材料力学性能的影响,探索合适的配方比例和工艺参数。结果：光固化胶粘剂最优配比为引发剂Darocur1173含量占1％,纳米SiO2含量占50％,基质树脂与稀释单体（Bis—GMA：TEGDMA）的比例为1：1。结论：制备的光固化胶粘剂配方的综合性能最优,可应用于临床。     

槲皮素提取条件的优选

目的优选槲皮素的提取工艺。方法采用浸渍法和回流法从仙人掌中提取槲皮素。选择溶剂种类、浓度、回流提取时间和仙人掌粉末粒度4个因素,并以L9(34)正交设计安排实验。结果最佳回流提取工艺为:过60目筛的干燥仙人掌粉末加95%甲醇回流3h。结论回流提取的效率高于浸渍提取。     

胃癌细胞体外药敏试验中的条件优化

目的:探讨胃癌细胞体外药敏试验中的适宜条件。方法:利用正交试验,取3个水平4个因素进行全面考察,筛选出最佳实验条件。结果:胃癌细胞体外药敏试验中应取(2~3)×104个/m l细胞密度,并在接种后立即加入10 PPC的抗癌药物,药物作用时间为72 h。结论:经正交试验筛选出的最佳试验条件可有效提高体外药敏试验的灵敏度,为临床个体化疗提供方法学依据。     

BCNU缓释微球制备条件的优化

目的以溶剂挥发-萃取法制备卡氮芥-聚乳酸羟基乙酸(BCNU-PLGA)缓释微球,对微球制备过程中的工艺条件进行优化选择。方法以正交实验设计对可能的影响因素,包括不同聚合度的聚乙烯醇(PVA)、PVA浓度、PLGA浓度、搅拌速度、甲基纤维素(MC)浓度进行了探讨。结果发现影响微球质量的因素,顺序从大到小依次为:PVA浓度、PLGA浓度、搅拌时间和MC浓度(A-B-D-C)。最佳实验方案为A2B1C2D3,即分散介质PVA浓度为1.5%,有机相中PLGA浓度为2%,搅拌时间为60min,MC浓度为0.05%。结论正交实验设计方法有助于选择更合适的制备条件。     

正交试验法筛选骨平巴布膏的基质处方

目的:筛选骨平巴布膏的基质处方。方法:采用正交试验法,以黏着力、剥离性、涂展性、膏体均匀性、皮肤追随性和赋形性为指标,各基质及其用量为因素和水平进行L₁₆(4⁵)试验,得出最优配比组成。结果:基质最佳配比为甘油∶明胶∶聚丙烯酸钠∶羧甲基纤维素钠∶水=5.0∶0.4∶1.4∶0.3∶4.0。结论:确定的基质处方实用可行。     

Alterations of molecular architectures in skeletal muscle plasma membranes of dehydrated and water-loaded mice

It is likely that orthogonal arrays (OAs) and caveolae seen on the replicas of freeze-fractured muscle plasma membranes are involved in maintaining osmotic homeostasis. Therefore, using the freeze-fracture technique, we examined the ultrastructural changes in OAs and caveolae of the skeletal muscle plasma membrane of dehydrated and water-loaded mice. In the muscle plasma membranes of 6 dehydrated and 6 water-loaded mice, caveolar distribution was not altered, and the densities of caveolae and OAs did not show statistically significant differences when compared with those in 6 control mice, although the skeletal muscles of water-loaded mice sometimes had muscle plasma membranes with extremely numerous OAs. In contrast, the muscle plasma membranes of dehydrated mice often revealed changes in the distribution of OAs, which existed in a group at the confined area of the muscle plasma membranes and were frequently accompanied by the aggregations of intramembranous particles (IMPs) around OAs. Thus, on the basis of the present study, we suggest that OAs in skeletal muscles as well as those in brain may play an important role in maintaining osmotic homeostasis of these organs under abnormal water balance.