56例成人腹股沟斜疝改良式修补术的临床分析

目的 探讨腹股沟斜疝改良式修补术的临床经验.方法 回顾性分析2001年6月至2005年6月收治的56例腹股沟斜疝患者的临床资料.结果 术侧阴囊轻微水肿12例,均在出院前治愈.56例患者全部治愈.随访6个月至5年均无复发.结论 腹股沟斜疝改良式修补术恢复了内环口的正常解剖结构,术中注意加强了对腹横筋膜缺损的修补,患者保持了腹股沟的正常解剖结构,治愈率高,有效降低了术后复发率,成本效应和生活质量优势明显,适合于基层医院对腹股沟斜疝患者的外科治疗.     

小柴胡汤对免疫抑制小鼠的免疫调节作用

目的:探讨小柴胡汤对免疫抑制小鼠免疫功能的影响.方法:BALB/c小鼠肌肉注射环磷酰胺建立免疫抑制小鼠模型,同时给予小柴胡汤,连续7日,并设免疫抑制模型及正常对照组.检测各组脾脏T淋巴细胞增殖能力及Till型(IL-2、IFN-r)和TH2(IL-4、IL-10)型细胞因子产生水平.结果:与正常对照小鼠比较,免疫抑制小鼠T细胞增殖能力明显下降,T淋巴细胞增殖能力及TH1和TH2型细胞因子产生均明显降低(P<0.01).给予小柴胡汤的正常小鼠T淋巴细胞增殖能力提高,TH1和TH2型细胞因子产生也有所增加,但与正常组比较没有显著差异(P>0.05).与免疫抑制小鼠相比较,给予小柴胡汤的免疫抑制小鼠的T淋巴细胞增殖能力及TH1和TH2型细胞因子的产生均明显增加,(P<0.01).结论:小柴胡汤可以有效地增强免疫抑制小鼠免疫功能.     

催乳方治疗产后缺乳的疗效观察

目的：观察催乳方治疗产后缺乳（气血虚弱型）的临床疗效，为催乳方的临床应用提供科学依据。方法：将108例产后缺乳的患者随机分为2组，治疗组60例以催乳方（北芪30、党参15、当归10、王不留行20（炒）、通草12、猪蹄1～2只，黄豆50、花生50）治疗；对照组48例以花生黄豆猪蹄汤治疗。1日1剂，分早、晚两次服用，7天为1疗程。对比观察两组就诊的第1天、第8天哺乳后4小时的一次泌乳量及临床疗效。结果：2组比较，治疗后第8d泌乳量（P〈0．001）及临床疗效（P〈0．05）差异均有统计学意义。结论：催乳方治疗气血虚弱型的产后缺乳具有较好疗效。     

柴芩承气汤减轻急性胰腺炎大鼠胰腺钙超载的机制研究

目的：观察柴芩承气汤（Chaiqinchengqi decoction,CQCQD）对急性胰腺炎大鼠胰腺组织肌浆网钙ATP酶（sarcoendoplasmic reticulum Ca2＋-ATPase,SERCA）mRNA表达的影响。方法：30只SD大鼠随机分成正常对照组、模型组及CQCQD组。采用蛙皮素腹腔注射法建立急性胰腺炎大鼠模型。逆转录聚合酶链式反应检测胰腺组织SERCA1和SERCA2 mRNA表达的变化;激光共聚焦显微镜检测腺泡细胞内钙离子浓度,并比较各组大鼠胰腺组织病理变化。结果：SERCA1 mRNA在正常胰腺和急性胰腺炎胰腺腺泡细胞均不表达。模型组与正常对照组比较,腺泡细胞SERCA2 mRNA表达下调,表达率为0.536（P=0.001）;CQCQD组与模型组比较,腺泡细胞SERCA2 mRNA的表达上调,表达率为1.803（P=0.035）。模型组大鼠细胞内钙离子的荧光强度高于正常对照组及CQCQD组（P〈0.05）;CQCQD组大鼠胰腺组织的病理评分低于模型组（P〈0.05）。结论：CQCQD可以通过上调SERCA2 mRNA表达,减缓胰腺细胞内钙超载,减轻胰腺组织的病理改变。     

五花舒肝汤治疗失眠52例疗效观察

[目的]观察五花舒肝汤、阿普唑仑治疗失眠的临床疗效和不良反应.[方法]将102例失眠患者随机分为二组,中药治疗者组用五花舒肝汤加减治疗,西药对照组用阿普唑仑治疗,4周为1疗程,观察二组患者失眠缓解情况、不良反应发生情况.[结果]中药治疗组治愈10例,显效14例,有效19例,无效9例;西药对照组治愈11例,显效16例,有效17例,无效6例;治疗组与对照组之间治愈率、显效率、总有效率均无明显差异(P＞0.05);不良反应中药治疗组9例,西药对照组19例,两组有明显差异(P＜0.05).[结论]中药治疗可以有效缓解失眠,与苯二氮卓类药物阿普唑仑疗效相当;但中药不良反应更少,易于被患者接受.     

中药治疗输卵管结扎术后遗症100例

目的：为观察和验证黄芪通脉汤对闭塞性动脉硬化（ＡＳＯ）患者血脂的调节作用。方法：选择ＡＳＯ患者３２例，应用黄芪通脉汤煎剂内服法治疗２个月，观察治疗前后血脂水平的变化。结果：治疗后患者血清高密度脂蛋白胆固醇升高、低密度脂蛋白胆固醇降低、动脉硬化指数下降（Ｐ＜０．０１）；血清胆固醇与甘油三酯、极低密度脂蛋白胆固醇、载脂蛋白（ａｐｏＡ１、ａｐｏＢ１００）均有变化（Ｐ均＜０．０５），ａｐｏＡ／ａｐｏＢ比值升高。结论：黄芪通脉汤具有明显的调脂作用。     

烧伤金黄液治疗Ⅱ度烧伤200例

烧伤金黄液治疗Ⅱ度烧伤２００例尚卫政，李燕凌，尚勇志自１９９０年９月，我们用烧伤金黄液外涂治疗Ⅱ度烧伤２００例，取得良好的效果。现报告如下。临床资料２００例患者，男１２５例，女７５例；年龄２个月～９０岁；烧伤时间２ｈ～４天，平均３２ｈ；浅Ⅱ度１２０例...     

Inhibitory Activity of Green and Black Tea in a Free Radical-generating System Using 2-Amino-3-methylimidazo[4,5-f]quinoline as Substrate

Green tea and black tea inhibit colon carcinogenesis in rats exposed to the cooked meat mutagen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ). In the present investigation, green tea, black tea and (-)-epigallocatechin gallate (EGCG) were shown to block the production of oxygen free radicals derived from IQ in the presence of NADPH-cytochrome P450 reductase. In kinetic studies using IQ as tbe substrate and DMPO as a free radical spin trap, EGCG increased the Km of the reaction without altering Vmax, suggesting competitive enzyme inhibition (Ki=9.% mUM). This was confirmed in spectrophotometric studies using cytochrome c as the substrate, in which EGCG acted as a competitive inhibitor of NADPH-cytochrome P450 reductase (Ki=9.7 μM). These results suggest that the inhibitory activities of green tea and black tea in electron spin resonance assays using IQ as the substrate for the rednctase are related to an indirect effect on the enzyme rather than via direct scavenging of the free radicals. The possible implications of these findings are discussed in the context of pathways involved in the activation and detoxification of IQ in the colon.     

Anticarcinogenic Activity of Green Tea Polyphenols

The main physiologically active polyphenol in green tea extractis (–)-epigallocatechin gallate (EGCG). Green tea extracthas an advantage over EGCG as a cancer chemopreventive agentfor humans, as is apparent from the Japanese custom of injestinggreen tea on a daily basis. Green tea extract similarly inhibitedprotein kinase C activation by teleocidin, a tumor promoter,as did EGCG. In addition, EGCG and green tea extract showedinhibitory effects on the growth of lung and mammary cancercell lines with similar potencies. An experiment using the estrogen-dependentMCF-7 cell line showed the mechanisms of action of these compoundsto be inhibiting the interaction of estrogen with its receptors.Considering our previous results of a single application ofEGCG to mouse skin inhibiting the specific binding of ³H-12-0-tetradecanoylphorbol-13-acetate(³H-TPA) and ³H-okadaic acid, we postulated that EGCG and compoundsin green tea extracts would block the interaction of tumor promoters,hormones and growth factors with their receptors: a kind ofsealing effect. The sealing effect would account for reversiblegrowth arrest, and may be induced by various kinds of compond.     

通窍活血汤对犬急性心肌缺血、心脏血流动力学及心肌耗氧量的影响

观察了通窍活血汤对犬急性心肌缺血、心脏血流动力学及心肌耗氧量的影响。结果表明：通窍活血汤可明显减轻心肌缺血程度（∑－ＳＴ），缩小经Ｎ－ＢＴ染色所显示的梗塞区；增加冠脉血流量，扩张冠脉血管；增加动脉血压、左室内压、ｄｐ／ｄｔ＿（ｍａｘ）、心输出量及左室作功，提高心肌收缩力；降低心肌耗氧量，改善氧的供需平衡。提示该方剂对心肌缺血有明显的保护作用。