HPLC测定整肠正气丸中和厚朴酚及厚朴酚的含量

目的:建立整肠正气丸中和厚朴酚及厚朴酚的检测方法。方法:色谱条件为C18色谱柱;柱温为室温;流动相:甲醇-水(75∶25);流速:1.0mL·min-1;检测波长:294nm。结果:和厚朴酚及厚朴酚线性范围分别为12.15～121.50μg·mL-1和22.25～222.50μg·mL-1,r分别为0.9998,0.9995;和厚朴酚加样回收率为98.47%(RSD=0.72%,n=6);厚朴酚加样回收率为98.30%(为RSD=0.88%,n=6)。结论:该方法简便,准确度高,可有效控制整肠正气丸的质量。     

Electrochemistry of magnolol and interaction with DNA

The electrochemical behaviors of magnolol have been studied at glassy carbon electrode using cyclic voltammetry, linear sweep voltammetry and chronocoulometry. Moreover, its interaction with DNA was investigated in solution by electrochemical methods and ultraviolet–visible spectroscopy. The experiment results indicated that the electrochemical oxidation of magnolol was an irreversible process with one proton and one electron transfer. The electron transfer coefficient (α) was calculated to be 0.441 ± 0.001. At the scan rate from 100 mV/s to 450 mV/s, the electrode process was controlled by the adsorption step and at the range of 600–950 mV/s the electrochemical oxidation was diffusion controlled process. The corresponding electrochemical rate constant (k_s) was 0.0760 ± 0.0001 s⁻¹. Through chronocoulometry experiment, the diffusion coefficient (D) and the surface concentration (Γ) were obtained as (3.76 ± 0.01) × 10⁻⁷ cm²/s and (2.98 ± 0.01) × 10⁻¹⁰ mol/cm². In addition, the interaction of magnolol and DNA was ascribed to be electrostatic interaction and the calculated association constant (β) and Hill coefficient (m) were 1.14 × 10⁵ M⁻¹ and 0.973. At last a sensitive and convenient electrochemical method was proposed for the determination of magnolol.     

Antioxidative and hepatoprotective effects of magnolol on acetaminophen-induced liver damage in rats

Acute liver failure (ALF), an often fatal condition characterized by massive hepatocyte necrosis, is frequently caused by drug poisoning, particularly with acetaminophen (N-acetyl-p-aminophenol/APAP). Hepatocyte necrosis is consecutive to glutathione (GSH) depletion and mitochondrial damage caused by reactive oxygen species (ROS) overproduction. Magnolol, one major phenolic constituent of Magnolia officinalis, have been known to exhibit potent antioxidative activity. In this study, the anti-hepatotoxic activity of magnolol on APAP-induced toxicity in the Sprague-Dawley rat liver was examined. After evaluating the changes of several biochemical parameters in serum, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) were elevated by APAP (500 mg/kg) intraperitoneal administration (8 and 24 h) and reduced by treatment with magnolol (0.5 h after APAP administration; 0.01, 0.1, and 1 μg/kg). Histological changes around the hepatic central vein, lipid peroxidation (thiobarbituric acid-reactive substance/TBARS), and GSH depletion in liver tissue induced by APAP were also recovered by magnolol treatment. The data show that oxidative stress followed by lipid peroxidation may play a very important role in the pathogenesis of APAP-induced hepatic injury; treatment with lipid-soluble antioxidant, magnolol, exerts anti-hepatotoxic activity. Our study points out the potential interest of magnolol in the treatment of toxic ALF. Yen-Yi Ho and Ming-Tsung Lai contributed equally to this work.     

Anti-platelet effect of the constituents isolated from the barks and fruits of<Emphasis Type="Italic">Magnolia obovata</Emphasis>

In the course of our work on anti-platelet constituents from plants, five phenolic compounds, magnolol, honokiol, obovatol, methyl caffeate, and syringin, were isolated from the methanol extracts of the barks and fruits of Magnolia obovata. The compounds were identified based on the spectroscopic data. Methyl caffeate was isolated for the first time from the genus Magnolia and it showed 3 approximately 4-folds higher potency than ASA. The activities of obovatol and honokiol were comparable to ASA. Magnolol and syringin showed only very mild inhibitory effects to all the stimulators.     

HPLC法测定暑湿清胶囊中厚朴酚及和厚朴酚的含量

目的:建立HPLC法测定暑湿清胶囊中厚朴酚与和厚朴酚含量的方法。方法:采用高效液相色谱法,色谱柱:Waters C18柱(250mm×4·6mm,5μm),流动相:甲醇-水(78:22),检测波长:294nm,流速:1·0mL·min-1。结果:厚朴酚的线性范围为0·08～4·06μg,平均加样回收率为98·23%(RSD=1·28%,n=6);和厚朴酚的线性范围为0·32～16·04μg,平均加样回收率为98·10%(RSD=0·54%,n=6)。结论:本方法简便、准确、可靠,灵敏度高,重现性好,可用于暑湿清胶囊的质量控制。     

厚朴温中丸质量标准的研究

目的:建立和完善厚朴温中丸的质量标准。方法:采用薄层色谱法对方中木香、厚朴、甘草进行定性鉴别;采用HPLC法测定了方中君药厚朴中厚朴酚与和厚朴酚的总含量。结果:鉴别结果理想,阴性对照无干扰;厚朴酚平均加样回收率为99.2%,RSD=1.2%(n=6);和厚朴酚的平均加样回收率为100.0%,RSD=2.5%(n=6)。结论:本方法快速简便,准确,可控制该制剂的质量。     

厚朴正交提取工艺的初步研究

[目的]探讨厚朴的提取工艺。[方法]利用正交设计法安排试验，以厚朴酚及厚朴酚的含量和收膏率为指标，考察提取工艺。[结果]最佳的工艺条件为8倍量70％乙醇，提取两次，1h／次。[结论]本研究利用最佳工艺能充分有效的提取厚朴药材中的有效成分。     

高效液相色谱法测定上消合剂中厚朴酚与和厚朴酚的含量研究

目的:运用反相高效液相色谱法测定上消合剂中厚朴酚及和厚朴酚的含量.方法:采用ZorbaxC18柱,甲醇-水(73:27)为流动相,检测波长为294nm.结果:厚朴酚的回收率为94.88%、RSD为0.97%(n=9);和厚朴酚的回收率为94.79%、RSD为1.55%(n=9).结论:简便、快速、重现性好,可作为上消合剂的含量测定方法.     

凹叶厚朴愈伤组织的诱导及其有效成分含量的比较

目的:研究凹叶厚朴愈伤组织的诱导、生长情况并比较愈伤组织内有效成分的含量。方法:采用单因素比较、正交设计试验,研究影响凹叶厚朴愈伤组织诱导和生长的各种因素;采用高效液相色谱法测定不同来源愈伤组织内有效成分的含量。结果:外植体脱分化形成愈伤组织的能力,以顶芽及茎段最好,雌蕊次之;愈伤组织诱导的最适培养基为B5+NAA0.1mg.L-1+6-BA1.0mg.L-1,而B5+NAA0.5mg.L-1+6-BA4.0mg.L-1对愈伤组织生长促进作用明显。以WPM为基本培养基诱导愈伤组织,继代后厚朴酚与和厚朴酚含量的总和可达0.1679%~0.2344%,但愈伤组织诱导率低,为33.08%~48.65%;培养条件为MS+NAA0.5mg.L-1+6-BA1.0mg.L-1时,愈伤组织诱导率为62.12%,继代后2酚总量为0.1686%。结论:本试验结果对凹叶厚朴愈伤组织的诱导及高产细胞系的筛选具有一定的参考价值。     

用高效液相色谱法同时测定藿香正气口服液中厚朴酚和厚朴酚与橙皮苷的含量

目的测定藿香正气口服液中有效成分厚朴酚、和厚朴酚与橙皮苷的含量而用于其质量控制。方法采用高效液相色谱(HPLC)的梯度洗脱法,在ODS柱上以甲醇-水(50∶50￣80∶20)为流动相进行分离,于294nm测定。结果橙皮苷、厚朴酚与和厚朴酚进样量分别在0.4400￣0.7920μg、0.0324￣0.1620μg和0.0392￣0.1960μg范围内与峰面积线性关系良好,相关系数为0.9997、0.9990和0.9991,平均回收率为100.1%、99.1%和101.3%。结论建立的HPLC方法对于所测定的三种有效成分具有良好的色谱分离性。该方法表现出了精密度好、准确度高、简便和稳定等特点,可用于制剂的质量控制。