Response of antioxidant enzymes, ascorbate and glutathione metabolism towards cadmium in hyperaccumulator and nonhyperaccumulator ecotypes of Sedum alfredii H

Hydroponics studies were conducted to investigate the antioxidant adaptations, ascorbate and glutathione metabolism in hyperaccumulating ecotype of Sedum alfredii (HE) exposed to high Cd environment, when compared with its nonhyperaccumulating ecotype (NHE). Exposure to Cd induced a burst of oxidative stress in both ecotypes which was evident by the sharp increase in hydrogen peroxide (H(2)O(2)) contents and lipid peroxidation. Buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, did not affect H(2)O(2) concentrations as well as growth of both ecotypes in the absence of Cd. However, compared with Cd application alone, BSO combined with Cd treatment caused a substantial augmentation of H(2)O(2) accumulation accompanied by a reduction in Cd concentrations in roots and leaves of HE at the end of treatment, which may rule out the possibility that GSH biosynthesis may play an important role as a signal of the stress regulation. No efficient and superior enzymatic antioxidant defense mechanisms against Cd-imposed oxidative stress existed in both NHE and HE, but the essential nonenzymatic components like ascorbic acid (AsA) and GSH played a prominent role in tolerance against Cd. Cadmium stimulated a notable rise in AsA concentration in both ecotypes soon after the application of treatment. A preferential Cd-stress response in HE was suggested to changes in the GSH pool, where acclimation was marked by increased GSH concentrations.     

高良姜防治糖尿病肢端疼痛的潜在靶点及作用研究

目的 用网络药理学的方法探讨高良姜治疗糖尿病肢端疼痛的分子作用机制.方法 通过中药系统药理学数据库与分析平台筛选高良姜的有效成分,用药物银行数据库、基因卡片数据库预测和筛选高良姜有效成分和糖尿病肢端疼痛相关靶点,用String数据库绘制靶点相互作用网络,借助生物学信息注释数据库对靶点进行基因本体和基于京都基因与基因组百...     

青天葵组培扩繁中的污染控制初探

目的研究青天葵组织培养中的污染问题,并提出防治措施。方法通过对污染的青天葵材料使用无菌水,生理盐水(0.9%NaCl),0.1%升汞,漂渍液和消毒片(次氯酸钠)清洗,对感染植物消毒效果进行研究,并考察了次氯酸钠的灭菌浓度及灭菌时间。结果青天葵组培物使用5%次氯酸钠清洗10min效果较好,可基本抑制污染,植物材料仍能正常生长;升汞灭菌虽可较彻底抑制污染,但对植物伤害较大,只用于对感染的球茎灭菌。结论通过污染控制研究,能提高青天葵组织培养的成功率。     

辽宁省不同地区苦碟子中总黄酮含量测定及比较研究

目的：建立苦碟子中总黄酮含量的定量方法;根据该方法测定辽宁省不同地区出产的苦碟子中总黄酮的含量并进行了比较研究。方法：紫外分光光度法。结果：该方法简单,专属性及重复性良好,可用于苦碟子中总黄酮的含量测定。结论：辽宁省朝阳、鞍山、锦州地区苦碟子中总黄酮的含量偏高,沈阳、丹东、本溪地区苦碟子中总黄酮的含量偏低。     

三叶青藤醇提物的镇痛抗炎作用

目的:研究三叶青藤醇提物的镇痛及抗炎作用。方法:采用小鼠热水缩尾法和醋酸扭体法观察镇痛作用;采用二甲苯致小鼠耳肿胀、角叉菜胶诱导大鼠足肿胀的试验模型观察抗炎作用;并测定角叉菜胶诱导大鼠足肿胀炎性渗出液中前列腺素E2(PGE2)的含量。结果:三叶青藤醇提物能明显提高小鼠的痛阈值,但未能减少小鼠的扭体次数。同时,三叶青藤醇提物能显著减轻小鼠耳肿胀和大鼠足爪肿胀反应,能明显减少大鼠角叉菜胶性炎症渗出液中PGE2含量。结论:三叶青藤醇提物没有明确的镇痛作用,但有显著的抗炎作用,其机制可能与抑制PGE2的合成有关。     

金钱草壳聚糖微球制备工艺研究

目的：对金钱草壳聚糖微球的制备工艺和包封率、粒径等特性进行初步研究。方法：以生物降解材料壳聚糖为载体,采用乳化一交联法制备金钱草壳聚糖微球,并采用正交试验优化制备工艺。结果：所制备的金钱草壳聚糖微球外观圆整,平均粒径为48．34μm,载药量为15．11％,包封率为59．55％。结论：所制备的金钱草壳聚糖微球重现性良好,载药量较高,制备工艺可行。     

4种豆科植物种子中氧化苦参碱的含量分析和其他3种生物碱的定性分析比较

目的采用HPLC法对豆科植物苦豆子、苦参种子、白刺花种子和砂生槐种子中主要有效成分氧化苦参碱的含量进行分析,并对其中的氧化槐果碱、槐定碱和苦参碱进行定性分析,为上述药用植物种子资源的有效利用提供依据。方法采用Li-chrospher和Phecda C8柱(150mm×4.6mm,5μm);流动相为0.05mol.L-1磷酸二氢钠溶液(含磷酸2mL.L-1)-甲醇-高氯酸钠(850∶150∶10);检测波长215nm;体积流量1.0mL.min-1。结果氧化苦参碱在0.2～1.2μg范围内与峰面积呈良好的线性关系;苦豆子和白刺花种子中氧化苦参碱平均含量为1.5%左右;苦参种子中氧化苦参碱的平均含量为2.5%;砂生槐种子中氧化苦参碱的含量最高,平均为4.69%。结论白刺花种子和砂生槐种子是较好的生产氧化苦参碱的潜在资源。     

Chemical constituents of Hainan Syzygium myrsinifolium (Hance) Merr. et Perry based on ultra-high performance liquid chromatography-time-of-flight tandem mass spectrometry

Syzygium myrsinifolium (Hance) Merr. et Perry is a unique plant resource on Hainan Island, and there are few reports on its chemical constituents. In the present study, we identified the major chemical constituents in the leaves of Syzygium myrsinifolium (Hance) Merr. et Perry using ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). The chemical constituents in Syzygium myrsinifolium (Hance) Merr. et Perry were identified rapidly based on the accurate relative molecular mass and combined with literature data and reference substances. A total of 20 compounds, including organic acids, phenolic acids, and flavonoids, were identified. Among these 20 compounds, four organic acids, eight phenolic acids, seven flavonoids, and one coumarin were reported from the leaves of Syzygium myrsinifolium (Hance) Merr. et Perry for the first time. The established method was rapid and accurate, and some chemical constituents in the leaves of Syzygium myrsinifolium (Hance) Merr. et Perry have been identified. This research provided an experimental reference for the study of the constituents and utilization of Syzygium myrsinifolium (Hance) Merr. et Perry.     

独活与混淆品牛尾独活、短毛独活的鉴别

目的鉴别独活与混淆品牛尾独活、短毛独活。方法采用性状、显微、薄层色谱法及液相色谱法鉴别。结果独活与混淆品牛尾独活、短毛独活的性状、显微特征、薄层色谱、液相色谱均有差异。结论本实验提供的鉴别方法,能准确地把独活与牛尾独活、短毛独活予以区别。     

苦碟子注射液治疗不稳定性心绞痛临床观察

目的：观察苦碟子注射液对不稳定心绞痛的疗效。方法：60例不稳定心绞痛患者在常规治疗基础上。给予苦碟子注射液每天20ml,14d后观察治疗效果。结果：治疗后I临床症状有效率为86-7％。心电图恢复总有效率为63-3％。全血粘度（rib）、红细胞压积（Hct）、纤维蛋白原（fb）。治疗前后差异均有统计学意义（P〈0-05）。结论：苦碟子注射液是治疗不稳定心绞痛可选用的有效药物之一。