乳疾灵颗粒辐照前后淫羊藿苷含量变化研究

摘 要 目的： 对比乳疾灵颗粒辐照前后淫羊藿苷含量差异。方法: 采用HPLC色谱法,色谱柱为Thermo ODS-2 HYPERSIL色谱柱(250 mm×4.6 mm,5 μm);流动相为乙腈-0.033 mol·L^-1磷酸二氢钾(30∶70);检测波长为265 nm;流速为1.0 ml·min^-1;柱温30℃。结果:淫羊藿苷在浓度3.44～34.40 μg·ml^-1范围内呈现良好的线性关系,r=0.999 7(n=6);平均回收率为98.18%,RSD=0.32%。乳疾灵颗粒辐照前后淫羊藿苷含量变化差异无统计学意义(P>0.05)。结论: 常规剂量照射处理对乳疾灵颗粒中淫羊藿苷影响较小。     

高效液相色谱法测定银花感冒冲剂中绿原酸含量

目的:建立测试银花感冒冲剂中绿原酸含量的高效液相方法,并对此方法进行系统的方法学验证,以确保应用该方法测试的结果准确、可靠。方法:采用色谱柱:Agilent TC-C18柱(4.6 mm×150 mm,5μm,Agilent,美国),流动相:甲醇-0.5%磷酸溶液(15∶85),检测波长为327 nm。结果:绿原酸在25~800μg/m L范围内线性关系良好,得到线性回归方程为Y=27.88X+2.128,相关系数为0.999 8(n=6);低、中、高浓度精密度的RSD值分别为0.59%、0.58%、0.44%;重复性RSD为0.26%;低、中、高浓度的加样回收率分别为99.24%、99.43%、99.75%;供试品溶液室温放置8 h稳定,RSD为0.29%。结论:本方法测定银花感冒冲剂中绿原酸的含量准确、可靠,操作简单、用时短,可用于银花感冒冲剂中绿原酸的含量测定。     

中西医结合优化治疗方案抑制IgA肾病肾纤维化的免疫机制〖JZ)〗〖HS)〗

目的:观察中西医结合优化方案治疗IgA肾病的临床疗效及改善肾纤维化的免疫机制。方法:选取2016年6月至2018年6月上海中医药大学附属曙光医院收治的IgA肾病患者40例作为研究对象,按照随机对照原则分为观察组和对照组,每组20例,观察组采用中药颗粒剂+氯沙坦的治疗方案,对照组采用中药模拟剂+氯沙坦的治疗方案,均治疗24周,治疗前后测定24 h尿蛋白定量、尿微量白蛋白/肌酐(MA/Cr)、血肌酐(Scr)、肾小球滤过率(eGFR)、白细胞介素6(IL-6)、转化生长因子β1(TGF-β1)。结果:观察组总有效率为80.0%,对照组为35.0%,2组患者比较差异有统计学意义(P<0.05)。观察组24 h尿蛋白定量、MA/Cr、Scr、eGFR较对照组显著改善(P<0.05)。观察组营养状态稳定,相关免疫机制尿IL-6得到显著改善(P<0.05),致肾纤维化因子尿TGF-β1也显著降低(P<0.05)。结论:中西医联合观察组较单纯西药组在降低蛋白尿、保护肾功能方面有明显优势,对IL-6的调节作用是中西医结合优化治疗方案改善IgA肾病肾纤维化的重要机制。     

Coated Langerhans cell granules in histiocytosis X cells

Histiocytosis X cells (HXC) and epidermal Langerhans cells (LC) are thought to be closely related because they share morphologic features and immunologic markers. One of these common markers, the LC granule, is an acknowledged morphologic criterion for the identification of these cell types, but its function and, thus, significance are as yet unknown. In this study, we report the presence of a fuzzy coat radiating from the cytoplasmic face of the LC granule membrane in HXC. This bristly coat is indistinguishable from that of coated vesicles and pits and, thus, represents a strong morphologic clue to the function of LC granules because coated structures have been shown to be involved in the selective transport of molecules in eukaryotic cells.     

肺痿冲剂方治疗肺肾两虚,气虚血瘀型特发性肺纤维化临床观察

目的:观察肺痿冲剂方治疗特发性肺纤维化肺肾两虚,气虚血瘀证患者的临床疗效。方法:将40例患者随机分为2组,治疗组以肺痿冲剂方治疗,对照组予口服金水宝胶囊,观察2组患者临床疗效、中医单项症状积分、肺功能、HRCT评分、圣乔治生活质量问卷以及6分钟步行试验。结果:在中医单项症状疗效积分(活动后气促、疲倦乏力)、肺功能中一氧化碳弥散量、蜂窝网评分及6分钟步行试验等方面,治疗组改善情况优于对照组,6个月、3个月疗程比较上述趋势更明显。结论:肺痿冲剂方治疗特发性肺纤维化肺肾两虚,气虚血瘀证,在改善临床症状、改善肺弥散功能、延缓蜂窝肺进展、改善生活质量及活动耐力等方面有较显著疗效。     

The RNA-binding protein and stress granule component ATAXIN-2 is expressed in mouse and human tissues associated with glaucoma pathogenesis

Polyglutamine repeat expansions in the Ataxin-2 (ATXN2) gene were first implicated in Spinocerebellar Ataxia Type 2, a disease associated with degeneration of motor neurons and Purkinje cells. Recent studies linked single nucleotide polymorphisms in the gene to elevated intraocular pressure in primary open angle glaucoma (POAG); yet, the localization of ATXN2 across glaucoma-relevant tissues of the vertebrate eye has not been thoroughly examined. This study characterizes ATXN2 expression in the mouse and human retina, and anterior eye, using an antibody validated in ATXN2^?/? retinas. ATXN2-ir was localized to cytosolic sub compartments in retinal ganglion cell (RGC) somata and proximal dendrites in addition to GABAergic, glycinergic, and cholinergic amacrine cells in the inner plexiform layer (IPL) and displaced amacrine cells. Human, but not mouse retinas showed modest immunolabeling of bipolar cells. ATXN2 immunofluorescence was prominent in the trabecular meshwork and pigmented and nonpigmented cells of the ciliary body, with analyses of primary human trabecular meshwork cells confirming the finding. The expression of ATXN2 in key POAG-relevant ocular tissues supports the potential role in autophagy and stress granule formation in response to ocular hypertension.     

REVIEWLaboratory investigation of platelet function

Advancement in the understanding of the mechanisms of platelet activation and the development of new techniques for studying platelet function have created the challenge of a wide range of options for investigating patients with suspected platelet disorders and for studying the effects of anti-platelet drugs. Novel classes of anti-platelet drug, such as adenosine diphosphate (ADP) receptor and glycoprotein IIb/IIIa receptor antagonists, are being developed and introduced into routine clinical practice. This review presents an overview of current techniques for laboratory investigation of platelet function in the light of these recent advances.     

HPLC测定不同厂家厚朴配方颗粒中厚朴酚与和厚朴酚的含量

摘 要 目的： 建立厚朴配方颗粒厚朴酚与和厚朴酚含量测定方法,比较不同厂家厚朴配方颗粒中厚朴酚及和厚朴酚的含量。方法: 应用HPLC法测定,色谱柱：HypersiL C₁₈(250 mm×4.6 mm,5 μm);流动相：A相为乙腈,B相为水,梯度洗脱;流速：1.0 ml·min^-1,柱温：25℃,检测波长：294 nm,进样量：20 μl。结果: 厚朴酚在0.873～26.190 μg·mL^-1范围内与峰面积呈良好的线性关系（r=0.999 5）,平均回收率为99.24%,RSD=2.00%（n=6）;和厚朴酚浓度在0.732～21.980 μg·mL^-1范围内与峰面积呈良好的线性关系（r=0.999 0）,平均回收率99.89%,RSD=1.33%（n=6）。不同厂家生产的厚朴配方颗粒中厚朴酚与和厚朴酚含量存在较大差异。结论:本方法简便、准确、重复性好,可用于厚朴配方颗粒中厚朴酚与和厚朴酚含量测定,不同厂家厚朴配方颗粒的质量差异提示规范药材种植、遴选、制定科学统一的配方颗粒生产工艺和质量控制标准的必要性。     

健肝乐颗粒联合拉米夫定治疗HBeAg阳性慢性乙型肝炎疗效分析

目的研究健肝乐颗粒联合拉米夫定治疗HBeAg阳性慢性乙型肝炎的临床疗效。方法选择HBeAg阳性慢性乙型肝炎148例患者的临床资料,按照治疗完成时间分为正常治疗组患者88例和延长治疗组60例。两组患者均联合使用健肝乐颗粒、拉米夫定连续治疗12个月,正常治疗组治疗停止,延长治疗组继续进行治疗方法治疗4个月。观察两组患者治疗12个月以及治疗完成后随访6个月时肝功能,乙型肝炎DNA、HBsAg、抗-HBs等指标定量检测,抗-HBe、HBeAg等指标半定量检测结果。结果两组患者年龄、HBeAg、丙氨酸氨基转移酶(ALT)、乙肝DNA对应比较,差异均无统计学意义(P均0.05)。治疗完成后以及治疗后随访6个月,两组患者HBeAg、HBsAg、ALT、乙肝病毒DNA均有一定的改善,但延长治疗组各项指标改善均优于正常治疗组,且两组患者治疗完成后以及治疗后随访6个月HBeAg转阴率、HBsAg转阴率分别比较,差异均有统计学意义(P0.05)。结论健肝乐颗粒联合拉米夫定治疗HBeAg阳性慢性乙型肝炎疗效显著,且治疗时间越长,疗效越显著。但仍需进一步研究确定。     

五维赖氨酸颗粒治疗小儿厌食症的疗效观察

目的观察五维赖氨酸颗粒治疗小儿厌食症的临床疗效。方法选取我院及宝安区松岗人民医院自2006年1月～2009年12月收治的厌食症患儿126例随机分为观察组（五维赖氨酸颗粒治疗组）和对照组（常规治疗组）各63例,治疗周期为4周,观察两组患儿治疗效果。结果观察组显效48例,有效12例,总有效率为95.2%;对照组显效24例,有效22例,总有效率为73.0%。两组比较差异显著（P〈0.01）;两组患儿在治疗过程中均未发现不良反应。结论五维赖氨酸颗粒治疗小儿厌食症效果理想,值得临床推广使用。