白黎芦醇对人口腔鳞癌KB细胞增殖和凋亡的影响及其机制

目的:探讨白藜芦醇对人口腔鳞癌KB细胞的增殖及凋亡影响。方法:应用MTT比色法检测Res对KB细胞体外生长的抑制作用;流式细胞仪测定细胞的周期和凋亡;Hoechst33258荧光染料染色,显示凋亡细胞形态;Western blot方法检测survivin、Caspase-3、Smac蛋白的表达。结果:Res能以剂量和时间依赖的方式抑制KB细胞生长,以剂量依赖的方式使KB细胞周期阻滞在S期、诱导细胞凋亡;Hoechst33258染色,可见细胞呈明显的凋亡特征;Western blot方法测得Res下调KB细胞的Survivin蛋白表达水平、增加KB细胞胞浆的Smac蛋白含量(P<0.01)、同时活化Caspase-3蛋白。结论:Res可抑制KB增殖,使细胞周期呈S阻滞并可诱导细胞发生凋亡,Survivin、Caspase-3和Smac基因参与了Res诱导KB细胞凋亡的作用。     

ARMH3-mediated recruitment of PI4KB directs Golgi-to-endosome trafficking and activation of the antiviral effector STING

1. Download : Download high-res image (270KB)
2. Download : Download full-size image

     

To fuse or not to fuse: The elderly patient with lumbar stenosis and low-grade spondylolisthesis. Systematic review and meta-analysis of randomised controlled trials

BackgroundThe optimum surgical intervention for elderly patients with lumbar spinal stenosis (LSS) and low-grade degenerative-spondylolisthesis (LGDS) has been extensively debated. We conducted a systematic review and meta-analysis of randomised-controlled-trials (RCTs) comparing the effectiveness of decompression-alone against the gold-standard approach of decompression-with-fusion (D + F) in elderly patients with LSS and LGDS.MethodsA systematic literature search was performed on published databases from inception to October-2021. English-language RCTs of elderly patients (mean age over-65) with LSS and LGDS, who had undergone DA or D + F were included. The quality and weight of evidence was assessed, and a meta-analysis performed.ResultsSix RCTs (n = 531; mean age: 66.2 years; 57.8% female) were included. There was no difference in visual-analogue-scale (VAS) scores of back-pain (BP) or leg-pain (LP) at mean follow-up of 27.4 months between both DA and D + F groups (BP: mean-difference (MD)0.24, 95%CI: ?0.38–0.85; LP MD:0.39, 95%CI: ?0.34–1.11). No difference in disability, measured by Oswestry-Disability-Index scores, was found between both groups (MD:0.50, 95%CI: ?3.31–4.31). However, patients in DA group had less hospital complications and fewer adverse events (total-surgical-complications OR:0.57, 95%CI: 0.36–0.90), despite a higher rate of worsening DS (OR:3.49, 95%CI: 1.05–11.65). No difference in BP or LP was found in subgroup-analysis of open-laminectomy compared to posterolateral-fusion (PLF) (BP: MD: ?0.24, 95%CI: ?1.80–1.32; LP MD:0.80, 95%CI: ?0.95–2.55).ConclusionsDA is not inferior to D + F in elderly patients with LSS and LGDS. DA carries a lower risk of hospital complications and fewer adverse events, however, surgeons should weigh these findings with the increased risk of DS progressing post-operatively.