Ginkgo biloba extract (GBE) and the vestibular system : A craniocorpographic and electronystagmographic assessment

The effect of Ginkgo Biloba Extract (GBE) on normal volunteers and vertiginous patients in two separate, randomised trials is described. The drug was administered orally as well as intravenously. Results were monitored using both electronystagmography and cranio-corpography. An analysis of both groups indicates a beneficial, “stabilising” effect of the compound on the vestibular system.     

蛇床子有效成份的提取与检测

目的：寻找理想的蛇床子有效成份的提取方法。方法：改进经典的提取方法，并采用荧光薄层析谱检测提取物进行方法学评价。结果：蛇床子总香豆素得率为6．72％，蛇床子总香豆素中主要成份蛇床子素含量达到15．26％。结论：采用本文方法提取的蛇床子有效成份得率较高，提取方法可靠，能有效地利用蛇床子药材资源，保证中药制剂的质量。     

HPLC测定芎芷香苏散流浸膏中甘草酸的含量

本文用ＨＰＬＣ测定芎芷香苏散流浸膏中甘草酸的含量，以乙腈－１．５ｍｏｌ／Ｌ冰醋酸水溶液为流动相，ＵＶ－２８０ｎｍ，在５０－２５０μｇ／ｍｌ范围内线性关系良好，ｒ＝０．９９９９，提取和测定方法简便，回收率高。     

鸡胚脊髓背角神经营养物质的HPLC纯化分析

目的　纯化鸡胚脊髓背角内的神经营养物质。方法　采用 Sephadex G- 2 0 0凝胶过滤层析法分离鸡胚脊髓背角内的神经营养物质 ,以鸡胚背根神经节细胞培养结合 MTT法检测各洗脱峰液的神经营养活性。所得活性组分用高效液相色谱技术 (HPL C)进一步分离 ,同前用 MTT法检测各洗脱峰液的神经营养活性。结果　经层析获得三个洗脱峰 ,其中第 2洗脱峰液促进培养神经细胞生长的活性最强 (P<0 .0 1) ,提示 2峰洗脱液含有神经营养物质。 HPL C后获得 7个洗脱峰。经 MTT法测定各峰洗脱液对鸡胚 DRG神经细胞的生长活性发现 F峰有明显的神经营养活性 (P<0 .0 5 )。结论　通过分离纯化获得了有较强活性的神经营养物质。     

On the active principles in Toh's alkaline tissue extracts which cause release of serotonin from blood platelets

Summary The release of serotonin from rabbit blood platelets by the alkaline extracts of tissue, originally described byToh in 1956, was re-examined. The extracts were found not only to cause the release of serotonin on incubation with platelets, but also to inhibit strongly the uptake into platelets of the amine added to the incubation medium. Hardly any apparent damage of platelets was, however, caused by the extracts. The activity of the extracts was dependent on temperature and varied with the tissue used. Extracts of kidney showed the greatest activity. Moreover, the activity was inhibited by the presence of glucose in the incubation medium but remained unaffected after pretreatment with trypsin and chymotrypsin.Based on these results, partial purification and chemical characterization of active principles in the alkaline kidney extract was attempted using extraction with organic solvents and thin-layer chromatography. The results obtained strongly suggest that the active principles are of acidic lipid nature and it is highly probable that they are mono- and di-enoic fatty acids set free by the alkali treatment used for tissue extraction. Such a view was supported by a model experiment in which authentic oleic and linoleic acid could liberate platelet serotonin on incubation but stearic and linolenic acid failed to do so.     

Eugenol and clove leaf oil: A survey of consumer patch-test sensitization

The potential of eugenol and of clove leaf oil, which contains a high concentration of eugenol, to induce delayed skin hypersensitivity or to elicit reactions due to pre-existing skin sensitization in man was evaluated by analysing patch-test data. Results from a total of 11,632 patch tests on eugenol itself, on various consumer products containing eugenol and/or clove leaf oil, and on fragrance blends containing eugenol and/or clove leaf oil were collected from fragrance and formulation companies. One instance of induced hypersensitivity and one instance of pre-existing sensitization were observed at eugenol patch-test concentrations of 5 × 10⁻² and 9 × 10⁻²% respectively. In both cases, subsequent patch testing showed skin responses to mixtures of eugenol and isoeugenol, but no further attempt was made to define the causative agent. The survey indicates that, at the concentrations present in consumer products, eugenol alone or as part of clove leaf oil has a very low potential either to elicit pre-existing sensitization (‘elicited’ reactions) or to induce hypersensitivity (‘induced’ reactions).     

银杏叶提取物透皮吸收的研究

目的:了解银杏叶提取物(GBE)的透皮吸收变化。方法:用紫外分光光度计测定GBE经皮吸收后接收液的260nm处吸光度值,计算出不同时间GBE经皮吸收的百分比。结果:银杏叶提取物(GBE)的透皮吸收效果可靠,接收液中GBE的浓度逐渐增高。结论:GBE具有重要的药理作用,可将其作成贴剂透皮吸收给药,预防、治疗心脑血管病等。     

银杏达莫注射液联合奥扎格雷治疗冠心病心绞痛临床观察

目的观察银杏达莫注射液联合奥扎格雷中西医结合治疗冠心病心绞痛的效果。方法将60例冠心病心绞痛病人随机分为两组,观察组28例给予银杏达莫注射液15 mL加5%葡萄糖或0.9%氯化钠,静脉滴注,1次/d,连用14 d;奥扎格雷80 mg,静滴,1次/d,连用14 d。对照组22例常规服用阿司匹林、硝酸酯类。结果观察组临床总有效率为93.4%,对照组总有效率为71.3%;观察组心电图总有效率为70.1%;对照组总有效率为57%。结论银杏达莫注射液联合奥扎格雷能明显减少心绞痛的发作次数及减轻胸闷、胸痛的症状。     

杜香提取物抑制荷瘤H22小鼠生长的实验研究

目的 探讨杜香提取物熊果酸的抗肿瘤作用.方法 采用水蒸气蒸馏法制备杜香提取物—熊果酸,将其作用于H22荷瘤小鼠模型,计算肿瘤抑制率.结果 剂量分别为0.25、0.5、1.0 mg/kg的熊果酸对H22荷瘤小鼠肿瘤抑制率分别为25.93%、44.39%和65.56%,与正常对照组比较差异显著(P＜0.05).结论 杜香提取物熊果酸能够抑制肿瘤细胞的生长.     

人面子叶提取物抑菌作用研究

目的:研究人面子叶石油醚、氯仿、乙酸乙酯及正丁醇部位的抑菌作用。方法:采用纸片扩散法进行抑菌试验研究。结果:人面子叶的乙酸乙酯部位对金黄色葡萄球菌、大肠杆菌及枯草芽孢杆菌抑菌作用最强,最小抑菌浓度(MIC)为每片0.117～0.234mg;正丁醇部位抑菌作用次之,MIC为每片0.496mg。结论:人面子叶乙酸乙酯部位抑菌作用较其它提取部位强,可为其后期开发提供理论依据。