杜香提取物抑制荷瘤H22小鼠生长的实验研究

目的 探讨杜香提取物熊果酸的抗肿瘤作用.方法 采用水蒸气蒸馏法制备杜香提取物—熊果酸,将其作用于H22荷瘤小鼠模型,计算肿瘤抑制率.结果 剂量分别为0.25、0.5、1.0 mg/kg的熊果酸对H22荷瘤小鼠肿瘤抑制率分别为25.93%、44.39%和65.56%,与正常对照组比较差异显著(P＜0.05).结论 杜香提取物熊果酸能够抑制肿瘤细胞的生长.     

人面子叶提取物抑菌作用研究

目的:研究人面子叶石油醚、氯仿、乙酸乙酯及正丁醇部位的抑菌作用。方法:采用纸片扩散法进行抑菌试验研究。结果:人面子叶的乙酸乙酯部位对金黄色葡萄球菌、大肠杆菌及枯草芽孢杆菌抑菌作用最强,最小抑菌浓度(MIC)为每片0.117～0.234mg;正丁醇部位抑菌作用次之,MIC为每片0.496mg。结论:人面子叶乙酸乙酯部位抑菌作用较其它提取部位强,可为其后期开发提供理论依据。     

不同厂家藿香正气水质量比较研究及分析

目的:比较目前市场上流通的5个厂家藿香正气水的质量状况。方法:对5个厂家5批次药品进行装量差异和乙醇含量等常规检查,采用改进的高效液相色谱(HPLC)法测定药品中厚朴酚与和厚朴酚的含量,色谱柱为SHIMADZU HPLC-20AT ODSC18(150mm×4.6mm)柱,流动相为乙腈∶水=60∶40(2%冰乙酸),检测波长294nm。结果:改进HPLC方法测得和厚朴酚(r=0.9998)与厚朴酚(r=0.9999)在1～100mg·L-1范围内线性关系良好,厚朴酚的平均回收率为99.02%,RSD=1.78%;和厚朴酚的平均回收率为99.15%,RSD=0.97%。不同厂家生产的藿香正气水装量差异、乙醇含量、厚朴酚与和厚朴酚总量均符合2005年版《中国药典》中相关规定,但厚朴酚与和厚朴酚的含量差异较大。结论:建议进一步研究生产工艺,稳定原料药,并制定出科学、统一的质量标准。     

藕节炭的TLC鉴别和浸出物含量测定

目的:对藕节炭药材的鉴别方法及浸出物进行研究。方法:以3-表白桦脂酸、β-谷甾醇为对照,采用薄层色谱(TLC)法对藕节炭药材进行定性鉴别;采用65%乙醇为溶剂测其浸出物的含量。结果:TLC斑点明显;浸出物测定方法可行。结论:TLC法可以鉴别藕节炭药材;不同地区市售藕节炭65%乙醇浸出物含量差异较大。     

Studies on the improvement of leucodepletion performance of the Haemonetics MCS+ for production of leucodepleted platelet concentrate

With the implementation of universal leucodepletion, an in-line, negatively charged LRF6H leucodepleting filter became an essential part of the Haemonetics MCS+ plateletpheresis system. A larger-scale (968) study using the standard protocol revealed a 2.79% leucodepletion failure rate (standard < 5 2 10 6 leucocytes per adult therapeutic dose). Factors influencing the efficacy of the filter were investigated. The pH of the filtrate was 7.0, the temperature 28°C and filtration rate 80 ml/min. Reduction of the filtration rate to 30 ml/min (784 doses) reduced leucodepletion failure to 0.38%. Measurement of the leucocyte count, pre- and post-filtration of the platelet products, revealed that donations from 1% of donors contained substantially larger numbers of leucocytes in pre-filter samples (300-1500/ w l) than in control samples (35-70/ w l). This number tends to increase progressively with subsequent donations in these individuals, leading to leucodepletion failure, whilst peripheral leucocyte counts remain normal. The new continuous filtration protocol (version C) using a less impact filter LRF-XL and a lower (7 ml/min) head pressure was also effective but failure still occurred twice on one of the donors who persistently showed high pre-filter count. We conclude that leucodepletion failures in the Haemonetics system are related to both donor leucocyte (i.e., being light and non-adherent) and operational/filter performance.     

Inhibition of Apoptosis in Human Neutrophils by Helicobacter pylori Water-Soluble Surface Proteins

Background: Helicobacter pylori infection in humans causes persistent neutrophil infiltration into the gastric mucosa. It is believed that a prolongation of neutrophil life-span could contribute to the pathogenesis of H. pylori infection. We therefore examined whether the water-soluble surface proteins of H. pylori can influence the apoptosis of neutrophils. Methods: After neutrophils were incubated with H. pylori water extract (HPWE), neutrophil apoptosis was evaluated by TUNEL assay, Hoechst 33342 staining, electron microscopy and ELISA for cytosolic oligonucleosome-bound DNA for up to 48 h. To investigate the regulatory mechanisms of neutrophil apoptosis associated with HPWE, mRNA expression and protein production of Fas, Fas ligand (FasL) and tumor necrosis factor receptor 1 (TNF-R1) were analyzed by RT-PCR, ribonuclease protection assay, Northern blot and Western blotting. Cell surface expression of these death factors was also measured by flow cytometry. Results: HPWE inhibited neutrophil apoptosis and cytotoxicity for up to 48 h. The mRNA and protein expression of FasL and the cell surface expression of Fas, FasL and TNF-R1 in HPWE-treated neutrophils were suppressed compared with the controls. Conclusion: The water-soluble surface proteins of H. pylori could suppress neutrophil apoptosis. This may be caused by the suppression of FasL expression in neutrophils and Fas, FasL and TNF-R1 expression on the surface of neutrophils.     

超高效液相色谱法同时测定藿香正气水中7种成分含量

利用超高效液相色谱同时测定藿香正气水中7种有效成分的含量。采用Acquity BEH C₁₈(2.1 mm×100 mm,1.7 μm)色谱柱,以乙腈-0.1%磷酸溶液线性梯度洗脱,柱温35 ℃,流速0.4 mL/min,分别在249,284,336 nm测定7种有效成分含量,12 min内完成测试。结果显示,甘草酸、欧前胡素、异欧前胡素、橙皮苷、厚朴酚、和厚朴酚、苍术素等7种成分在各自线性范围呈良好的线性关系(r>0.999 9),加样回收率在96.16%~103.4%之间。     

小牛血去蛋白提取物联合甲钴胺超声雾化对LASIK术后角膜神经修复的影响

目的:探讨小牛血去蛋白提取物联合甲钴胺超声雾化对LASIK术后角膜神经修复的影响。方法:将本院行LASIK术的78例(146眼)患者随机分为对照组和观察组,均给予小牛血去蛋白提取物治疗,仅观察组加用甲钴胺超声雾化治疗;观察两组的术前,术后1周、1和6个月的角膜指标(中央知觉、神经纤维密度和内皮计数),一般指标(视力、泪膜破裂时间、基础泪液分泌和前房房水的闪光)和泪液中的生化指标水平[肿瘤坏死因子(TNF-α)、白细胞介素2(IL-2)和神经生长因子(NGF)]及用药期间的不良反应。结果:观察组术后1周的闪光、TNF-α、IL-2和NGF均优于对照组;术后1个月中央知觉、神经纤维密度、视力、泪膜破裂时间、闪光和NGF均优于对照组;术后3个月的中央知觉、神经纤维密度、泪膜破裂时间、基础泪液分泌和闪光均优于对照组,以上均有统计学差异。两组的不良反应率较低,且无统计学差异。结论:小牛血去蛋白提取物联合甲钴胺超声雾化可促进LASIK术后的神经修复,尤其是术后远期的效果较好,同时还可促进视力恢复和泪膜稳定性,可能与降低炎症反应并促进NGF分泌有关。     

刺五加配伍栀子治疗老年抑郁障碍的随机双盲对照研究

目的分析刺五加配伍栀子治疗老年抑郁障碍的临床疗效。方法选取2010年4月～2012年4月收治的老年抑郁障碍患者76例作为研究对象,将其随机分为观察组与对照组,各38例。对照组患者使用常规治疗,观察组患者使用刺五加配伍栀子治疗,以6周为1个疗程。比较两组患者的抑郁评分及治疗效果、认知功能评分、活动状态和生活质量评分差异。结果观察组接受治疗后的抑郁评分及治疗效果均明显优于对照组（P〈0.05）;观察组接受治疗后的认知功能评分明显高于对照组（P〈0.05）;观察组接受治疗后的活动状态和生活质量评分明显高于对照组（P〈0.05）。结论刺五加配伍栀子治疗老年抑郁障碍可以有效降低患者的抑郁评分,提高治疗效果,改善患者认知功能、活动状态及生活质量。     

加味不换金正气散灌肠治疗溃疡性结肠炎临床疗效观察

目的：观察并评价不换金正气散灌肠治疗溃疡性结肠炎（Ulcerative Colitis,UC）的临床疗效。方法：采用随机对照试验（RCT）研究方法观察加味不换金正气散灌肠疗法对UC临床治疗效果。将60例患者随机平均分为2组,两组患者均在口服美沙拉嗪缓释片基础上,治疗组给予加味不换金正气散煎剂保留灌肠,每晚1次;对照组给予柳氮磺吡啶肠溶片保留灌肠,每晚1次;持续治疗8周。分别记录并比较两组的临床症状、粪常规、内镜下肠黏膜病变改善情况、临床缓解时间及不良反应。结果：完成试验者55例（治疗组28例,对照组27例）,不良反应发生率在治疗组和对照组中,差异无统计学意义。两组患者临床症状缓解、肠黏膜疗效比较、临床活动指数等方面治疗组均优于对照组（P〈0.01或P〈0.05）。结论：加味不换金正气散治疗溃疡性结肠炎有较好的临床疗效,而且能够显著修复肠道黏膜。