急性脑梗死患者治疗前后血浆Hcy、CGRP和ET-1检测的临床意义

目的：探讨了急性脑梗死患者治疗前后血浆Hcy、CGRP和ET-1水平的变化及意义。方法：应用放免法和ELISA对33例急性脑梗死患者进行了治疗前后血浆Hcy、CGRP和ET-1的检测,并与35名正常健康人作比较。结果：在治疗前,急性脑梗死患者血浆Hcy、ET-1水平非常显著地高于正常人组（P〈0.01）,而CGRP水平则显著地低于正常人组（P〈0.01）;治疗后,则与正常人组比较无显著性差异（P〉0.05）。结论：检测急性脑梗死患者血浆Hcy、CGRP和ET-1水平的变化对了解病情、观察预后均有重要的临床价值。     

早期糖尿病肾病患者血清同型半胱氨酸和尿微量白蛋白的相关性研究

[目的]测定2型糖尿病早期肾病患者血清同型半胱氨酸（Hcy）水平,探讨其与尿微量白蛋白的相关性。[方法]应用东芝120全自动生化分析仪用循环酶法检测62例糖尿病患者的血清Hcy浓度,其中早期糖尿病肾病组32例,单纯糖尿病组30例,并以30例健康体检者作为对照组。采用免疫比浊法测定尿微量白蛋白水平。[结果]早期糖尿病肾病组及单纯糖尿病组血清Hcy水平高于对照组（P〈0．05）,早期糖尿病肾病组血清Hcy水平与单纯糖尿病组比较有统计学差异（P〈0．05）,早期糖尿病肾病组血清Hcy与尿微量白蛋白呈正相关。[结论]血清Hcy对糖尿病早期肾损伤具有重要的诊断价值。     

Fortification of flours with folic acid reduces homocysteine levels in Brazilian women

Our hypothesis is that the fortification of flour with folic acid contributes to the reduction of plasma homocysteine (Hcy). We conducted a cross-sectional study covering 2 periods, before and after fortification (2002-2003 and 2008-2009, respectively), to assess the influence of the consumption of corn and wheat flours prefortification and postfortification with folic acid on Hcy levels and other biomarkers. In the total, 93 women (38 prefortification and 55 postfortification) were included. Levels of lipids and glucose, total Hcy and serum folate, and cobalamin were determined using commercial kits by colorimetric method, competitive immunoassay, and chemiluminescence, respectively The participants' average age was 48.1 ± 9.5 years for the prefortification group and 39.1 ± 4.1 years for the postfortification group (P < .001) but adjusted statistical tests by age. Both groups presented obesity class 1. In the prefortification group, 71.1% (n = 27) of women had a dietary intake of folate, which was lower than the current recommended for adults (<400 μg/d), whereas in the postfortification group, only 16.4% (n = 9) of women had lower intakes than recommended. In the prefortification group, 42.1% (n = 16) of women had hyperhomocysteinemia (>10 mmol/L) compared with only 9.1% (n = 5) in the postfortification group. Moreover, statistically significant differences were found between the 2 groups in total cholesterol, high-density lipoprotein, triglycerides, and dietary fiber. Our findings corroborate the hypothesis that fortification of wheat and corn flours with folic acid can possibly be associated with lower concentrations of plasma Hcy, providing probable greater cardiovascular protection in this group.     

益肾活血胶囊对不稳定型心绞痛患者Hcy及ET、TNF-α、NO的影响

观察益肾活血胶囊对不稳定型心绞痛患者血液同型半胱氨酸(Hcy)、内皮素(ET)、肿瘤坏死因子α(TNF-α)、一氧化氮(NO)水平的影响。方法:将40例患者随机分为治疗组和对照组各20例,两组患者均采用常规西药治疗,治疗组同时加用益肾活血胶囊,对照组加用维生素B12和叶酸,疗程均为4周。结果:治疗组可显著降低冠心病患者Hcy、ET、TNF-α水平,并升高NO水平(P#0.05,P#0.01)。结论:益肾活血胶囊不仅能够降低不稳定型心绞痛患者Hcy水平,而且能够改善不稳定型心绞痛患者内皮功能,对于高半胱氨酸所致心绞痛的治疗效果明显高于对照组。     

血浆同型半胱氨酸、脂蛋白（a）水平与冠心病的相关性研究

目的探讨血浆同型半胱氨酸(HCY)、脂蛋白(a)[LP(a)]的变化与冠心病(CHD)的关系。方法将233例CHD患者分为三组,无症状型CHD组55例,稳定型心绞痛组95例,心肌梗死组83例,50名健康者作为对照组,同时根据冠脉造影进一步将观察对象分为一支病变组(93例),双支病变组(81例)和多支病变组(59例)分别在HITACHI-7600-020全自动生化分析仪上测定血浆Hcy及LP(a)的水平。结果冠心病患者血浆Hcy、LP(a)的水平均比健康对照组明显增高,二者有显著差异(P<0.01,P<0.05),急性心肌梗死组高于稳定型心绞痛组,稳定型心绞痛组高于无症状型CHD组;且血浆Hcy、LP(a)水平随病变支数增加而增高。结论CHD患者血浆Hcy、LP(a)水平明显增高,且与冠状动脉病变程度及冠脉病变支数呈正相关,是冠心病的独立危险因素。     

Analysis of cell-cycle kinetics and sulfur amino acid metabolism in methionine-dependent tumor cell lines; the effect of homocysteine supplementation

Methionine dependence is a feature unique to cancer cells, exhibited as inability to grow in a methionine-depleted environment supplemented with homocysteine, the immediate metabolic precursor of methionine. This study explores the effect of methionine depletion and homocysteine supplementation on the viability, sulfur amino acid metabolism and cell-cycle kinetics of normal and cancer cells, as well as their ability to recover from the treatments. An array of cells including hepatomas (HTC, Phi-1), prostate adenocarcinomas (PC-3) and transformed (3T3) and normal (HS-27) fibroblasts, has been used aiming to evaluate the importance of tissue specificity. All cell lines proliferated well in methionine-complete media (M+H-), whilst only the normal fibroblasts HS-27 grew in methionine-depleted homocysteine-supplemented media (M-H+). None of the tested cell lines were able to grow in media without methionine or homocysteine (M-H-). HTC was the only cell line that did not recover from the M-H+ treatment whilst PC-3 did not recover from the M-H- treatment. Methionine and homocysteine depletion (M-H+ and M-H-) were found to induce arrest at different phases of the cell cycle, depending on the cell line: the methionine-dependent HTC, PC-3 and 3T3 arrested at the S and G2/M phase, whilst Phi-1 and the methionine-independent HS-27 accumulated in the G1 phase. The cell-cycle kinetics showed that the observed blockades were reversible. The information resulting from these studies is important for not only the behavior of cancer cells, but also for appreciating the potential of developing cancer therapies based on methionine-depletion strategies.     

缺血性脑血管病患者颈动脉斑块与TXB2、OxLDL、Lp(a)、Hcy相关性分析

目的探讨缺血性脑血管病的颈动脉斑块与TXB2、OxLDL、Lp(a)、Hcy的相关性,以更好的对该病进行预防、治疗。方法35例急性缺血性卒中患者和20名正常对照组行颈动脉血管彩色多普勒超声检测颈动脉斑块,采用ELISA双抗体夹心法测定血浆TXB2、OxLDL、Lp(a),采用荧光偏振免疫分析法测定Hcy的含量,两组相比,并作相关性分析。结果斑块最大厚度、血浆TXB2、OxLDL、Lp(a)、Hcy的含量均高于正常对照组(P<0.01)。斑块最大厚度与TXB2、OxLDL、Lp(a)呈正相关。结论颈动脉斑块,血浆TXB2、OxLDL、Lp(a)、Hcy可能与缺血性脑血管病相关;TXB2、OxLDL、Lp(a)可能参与促进颈动脉粥样硬化斑块的形成。     

慢性肾功能不全与Hcy水平的关系

目的探讨慢性肾功能不全患者与血清同型半胱氨酸(Hcy)水平的关系。方法运用循环酶法技术测定46例慢性肾功能不全患者血清同型半胱氨酸(Hcy)水平,以38例正常人为对照组,进行t检验及线性相关分析。结果慢性肾功能不全组Hcy水平(24.4±10.7μmol/L)明显高于对照组(7.4±3.8μmol/L)(P<0.01),从相关分析看,慢性肾功能不全患者Hcy浓度随着BUN,CREA浓度的增高而增高。结论慢性肾功能不全患者普遍存在高Hcy血症,而且随着肾功能损伤的程度加深,并发症的不断出现,Hcy结果也逐渐增高。     

Alteration in the redox state of plasma in heart-transplant patients with moderate hyperhomocysteinemia

Hyperhomocysteinemia has recently been suggested to contribute to the progression of the so-called chronic rejection or cardiac allograft vasculopathy (CAV) in heart-transplant patients in which the major determinant of the increase in homocysteine (Hcy) was the progressive decline of renal function. The exact mechanisms of tissue injury by Hcy is unknown, but some aspects of its toxicity have been related to its capacity for altering the redox state of plasma and forming protein adducts by intermediate lactone. To study the relationships between Hcy levels and variations in the redox state governed by thiols, plasma levels of Hcy, cysteine, glutathione, cysteinylglycine, and corresponding disulfides and protein-mixed disulfides were evaluated in subjects with moderate hyperhomocysteinemia represented by heart-transplant patients with (HTRF) and without (HT) renal failure, as well as patients with renal failure of different origin (RF), and compared with those of a control group (C) of normal subjects matched for age and sex. Plasma levels of Hcy and the corresponding protein mixed disulfides increased progressively in HTs, RFs, and HTRFs with respect to control. These changes were correlated with cysteine variations (as cystine and protein-mixed disulfides) but not with glutathione or cysteinylglycine that varied only as disulfides with a similar tendency. Moreover, an alteration in the plasma redox was evidenced by the decrease in thiol/disulfide ratios of cysteine, Hcy, and cysteinylglycine. In all groups, cysteine was directly correlated with Hcy but not with glutathione or cysteinylglycine, which in turn were correlated each other. Therefore levels of plasma cysteine were more linked to Hcy than to metabolism of glutathione. The clinical meaning of cysteine changes remains undefined and requires further study.     

血浆同型半胱氨酸与2型糖尿病大血管病变相关关系的研究

目的:血浆同型半胱氨酸与2型糖尿病大血管病变相关关系的研究.方法:对22例健康成人、23例糖尿病无大血管并发症患者、31例有糖尿病大血管病变患者进行血浆同型半胱氨酸(Hcy)水平检测.结果:糖尿病各组血浆Hcy高于正常对照组,有大血管病变组又高于无大血管病变组,糖尿病伴大血管并发症组高Hcy血症发生率为61.9%,明显高于正常对照组4.55%和无大血管并发症组的21.74%.结论:空腹高Hcy血症与2型糖尿病大血管病变呈相关关系.