Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein)

Background

Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy.

Relation of age and smoking to serum levels of total testosterone and dehydroepiandrosterone sulfate in aged men

Background:   Hormonal factors have been extensively investigated in the area of geriatric medicine in the search for potential anti-aging agents or useful biomarkers for senescence in men. However, inconsistent results have been published so far concerning the relation of anthropometric and life-style factors to endocrine factors. To confirm the relationships between epidemiological parameters and sex hormone levels, we examined the relation of age and smoking to serum levels of total testosterone (T) and dehydroepiandrosterone sulfate (DHEA) in aged men.
Methods:   The subjects included men aged 50–74 years, 40 current smokers and 27 never smokers. Serum levels of T and DHEA were assayed with a radioimmunoassay kit.
Results:   Serum T did not decrease with age, and was significantly higher in smokers than for non-smokers. Serum DHEA decreased with age more sharply in non-smokers than for smokers.
Conclusion:   These data suggest that serum DHEA decreases with aging, but serum T does not, and that serum levels of these hormones are influenced by cigarette smoking.     

地佐辛复合硫酸镁治疗腰硬联合麻醉中寒颤反应的观察

目的：观察地佐辛复合硫酸镁在治疗腰硬联合麻醉中寒颤反应的效果。方法以发生寒颤反应的60例患者随机分组以实验组（地佐辛复合硫酸镁）与常规治疗对照组（曲马多复合氟哌利多）进行疗效比较。结果实验组与对照组疗效接近,但不良反应较少。结论佐辛复合小剂量硫酸镁可安全用于治疗腰硬联合麻醉后患者发生寒颤且具有镇静作用较好、恶心、呕吐发生率较低等优点。     

不同浓度葡聚糖硫酸钠对小鼠炎症性肠病模型建立及其致病相关免疫因子表达的影响

目的 探讨饮用不同浓度的葡聚糖硫酸钠(dextran sulfate sodium, DSS)对于建立小鼠炎症性肠病(inflammatory bowel disease, IBD)模型及其致病相关免疫因子表达的影响。方法 雄性C57BL/6J小鼠随机分为对照组和不同浓度的DSS饮用组(3%、5%、7%)。观察小鼠的大便性状,体重和生存时间。饮用后的第6天处死各组小鼠,观察结肠大体形态并评分;取病变处进行石蜡包埋病理切片,苏木素伊红染色并进行病理组织学评分;定量PCR检测各组脾细胞免疫因子表达情况。结果 饮用3%、5%、7%浓度DSS的小鼠在第6天均有不同程度溃疡形成,成模率随着DSS浓度提升而增加,但是小鼠死亡率也相应增加。定量PCR结果表明促炎因子(TNF-α、IFN-γ和IL-17A)的表达水平与DSS浓度成正相关,而抑炎因子(IL-4和IL-10)以及调节性T细胞相关的转录因子Foxp3的表达水平与DSS浓度成负相关关系。结论 给予小鼠5%浓度的DSS溶液饮用有助于高效经济地建立小鼠IBD模型,为进一步研究IBD的发病机理、生物学特性、干预因素等打下了重要基础。     

Aluminum phosphide (celphos) poisoning in children: A 5-year experience in a tertiary care hospital from northern India

Background:

Aluminum phosphide (ALP) (celphos) is an agricultural pesticide commonly implicated in poisoning. Literature pertaining to the clinical manifestations and treatment outcome of its poisoning among children is limited.

Materials and Methods:

A retrospective chart review was conducted of the medical records of 30 children aged less than 14 years admitted to pediatric intensive care unit (PICU) of a tertiary care hospital in northern India. Demographic, clinical, and laboratory parameters were recorded. The outcome was categorized into “survivors” and “nonsurvivors.”

Results:

The Mean (SD) age of the enrolled children [19 males (63.3%)] was 8.55 (3.07) years. Among the 30 children, 14 (46.67%) were nonsurvivors and the rest 16 (53.33%) were survivors. Nonsurvivors had ingested significantly higher doses of ALP (P < 0.001), and showed higher time lag to PICU transfer (P 0.031), presence of abnormal radiological findings on chest skiagram (P = 0.007), and a higher Pediatric Risk of Mortality (PRISM) III score (P < 0.001) at admission. Use of magnesium sulfate was associated significantly with survival [odds ratio (OR) (95% CI): 0.11 (0.02-0.66); P 0.016].

Conclusion:

The present study highlights that survival among children with ALP poisoning is predicted by dose of ALP ingestion, time lag to medical attention, and higher PRISM score at admission. Use of magnesium sulfate could be associated with better survival among them.     

Ionized magnesium and calcium concentration and their ratio in equine plasma samples as determined by a regulatory laboratory compared to a clinical reference laboratory

Magnesium sulfate (MgSO₄) was administered to calm competition horses. We evaluated the impact of regulatory requirements for the handling of blood samples on plasma ionized magnesium (iMg), ionized calcium (iCa), the iMg to iCa ratio, and pH. We hypothesized that iCa, iMg. and iMg/iCa would be similar among storage and collection methods. Four blood samples were collected from each of 50 horses on the same day: Group 1 – collection in a heparinized syringe and processed within hours in a clinical laboratory; Group 2 – collection into a plasma separator tube (PST) centrifuged just prior to analysis, and plasma processed as in (1); Group 3 – collection into a PST, refrigerated, shipped via overnight carrier to the United States Equestrian Federation (USEF) Equine Drug Testing and Research laboratory, centrifuged just prior to analysis, and plasma processed; and Group 4 – as in Group 3, but stored frozen at ?80°C for 90 days, thawed, and plasma processed as in Group 3. Results for iMg/iCa are unit‐less, adjusted iMg for potential influence of plasma protein and iCa, and highly correlated with iMg pH (r = ?.933; P < 0.01). Samples processed immediately in a clinical reference laboratory had the greatest iMg/iCa. Both iMg/iCa and pH predictably decreased after freezing (P < 0.001). These data suggest that the iMg/iCa mirrors alterations in iMg regardless of storage and collection methods. This understanding can facilitate the development of a regulatory threshold for the control of the nefarious use of magnesium sulfate in competing horses, and an understanding of potential changes to iMg/iCa with storage of B samples.     

硫酸镁联合孟鲁司特纳对重度哮喘患儿肺功能及呼吸动力学的影响

目的 分析硫酸镁联合孟鲁司特钠对重度哮喘患儿肺功能及呼吸动力学的影响。方法 选取2018 年6 月～2020 年6 月本院收治的78 例重度哮喘患者,以双盲随机抽样法分组,每组各39 例。对照组采用硫酸镁治疗,实验组采用硫酸镁联合孟鲁司特钠治疗,比较两组临床疗效、肺功能指标、呼吸动力学指标、不良反应发生率。结果 实验组临床总有效率（94.87%）较对照组（64.10%）高;治疗2 周后,实验组FEV1、PEF、FVC、Cst 均较对照组高;治疗2 周后,实验组Pawm、Plat、R 均较对照组低,差异均有统计学意义（P＜0.05）。两组不良反应总发生率比较,差异无统计学意义（P＞0.05）。结论 硫酸镁联合孟鲁司特钠可有效缓解重度哮喘患者临床症状,改善肺功能及呼吸动力学参数,且不良反应较少,值得借鉴。     

三痹汤治疗膝骨性关节炎

目的:观察三痹汤治疗膝骨性关节炎(knee osteoarthritis,KOA)的临床疗效。方法:120例KOA患者随机平均分为治疗组和对照组。治疗组给予三痹汤,对照组给予硫酸氨基葡萄糖胶囊。治疗后,比较两组患者临床疗效,临床症状疗效,视觉模拟评分(visual analogue scale,VAS),西安大略和麦克马斯特大学骨关节炎指数(Western Ontario and McMaster university of orthopedic index,WOMAC)评分,健康调查简表(the MOS item short from health survey,SF-36)评分及不良反应。结果:治疗组有效率为86.7%(52/60),对照组为60.0%(36/60),两组有效率比较,差异有统计学意义(P<0.05)。治疗后,两组临床症状评分比较,治疗组无效例数显著少于对照组,差异均有统计学意义(P<0.05)。治疗后,两组患者VAS、WOMAC评分均低于治疗前,治疗组治疗后低于同期对照组,差异均有统计学意义(P<0.05)。治疗后,两组患者SF-36评分均高于治疗前,治疗组治疗后高于同期对照组,差异均有统计学意义(P<0.05)。治疗组不良反应率为5.00%,对照组为16.67%,两组不良反应率比较,差异有统计学意义(P<0.05)。结论:三痹汤治疗KOA临床疗效显著,可显著改善患者临床症状,降低VAS、WOMAC评分,提高SF-36评分,且无明显不良反应。     

褐藻酸硫酸酯凝胶剂的质量考察及安全性研究

目的 制备褐藻酸硫酸酯凝胶剂,并对其进行质量考察及安全性评价。方法 以褐藻酸硫酸酯为有效成分制备凝胶剂,从外观、装样量、酸碱度、粘度、有效成分含量及微生物限度方面对凝胶剂质量进行考察,并对其生物安全性进行评价,包括细胞毒性、刺激性及致敏性。结论 制备得到了性状良好、性质稳定的褐藻酸硫酸酯凝胶剂,并且具有较高的生物安全性,为后期褐藻酸硫酸酯药物或医用功能制品的开发奠定了基础。