棓丙酯注射液有关物质检查中没食子酸测定方法的改进

目的:改进棓丙酯注射液有关物质检查中没食子酸含量的测定方法。方法:采用HPLC法,Agilent ZORBAX SB-C₁₈柱(4.6 mm×250 mm,5μm),以甲醇-水(用磷酸调节pH值至3.0)为流动相,梯度洗脱,流量1.0 mL·min^-1,检测波长为272nm。结果:改进后的方法能将没食子酸峰与辅料峰完全分离。结论:改进后的没食子酸测定方法更准确,可靠。     

Attenuation of beryllium induced hepatorenal dysfunction and oxidative stress in rodents by combined effect of gallic acid and piperine

We determined a minimum effective dose of gallic acid (3,4,5-trihydroxy benzoic acid; 50 mg/ kg, i.p.) and piperine (10 mg/kg, p.o.) through their therapeutic potential and further evaluated them individually and in combination against beryllium-induced biochemical alterations and oxidative stress consequences in female albino rats. The administration of beryllium altered blood biochemical variables by significantly depleting hemoglobin, albumin and urea, whereas it enhanced bilirubin and creatinine. The release of serum transaminase, lactate dehydrogenase and gamma-glutamyl transpeptidase was significantly greater, and was concomitant with a decrease in serum alkaline phosphatase. A significant increase in lipid peroxidation and a decrease in glutathione, superoxide dismutase and catalase in the liver and kidney was an indication of oxidative stress due to beryllium exposure. Individual administration of gallic acid and piperine moderately reversed the altered biochemical variables, whereas the combination of these was found to completely reverse the beryllium-induced biochemical alterations and oxidative stress consequences. We concluded that gallic acid exerts a synergistic effect when administered with piperine and provides a more pronounced therapeutic potential in reducing beryllium-induced hepatorenal dysfunction and oxidative stress consequences.     

Leaves of Cassia tora as a novel cancer therapeutic – An in vitro study

Cassia tora Linn (Leguminacea) is a medicinal plant traditionally used as laxative, for the treatment of leprosy and various skin disorders. Preliminary phytochemical analysis of leaf showed the presence of polyphenols (3.7 mg gallic acid equivalent per gram dried leaves). The presence of phenolic compound prompted us to evaluate its antioxidant and antiproliferative potential. In the present study C. tora methanolic leaf extract (CTME) was evaluated for its nitric oxide scavenging activity and reducing power assays using Rutin and BHT as standards. The extract was studied for its lipid peroxidation inhibition assay using rat liver and brain. In all assays, a correlation existed between concentration of extract and percentage inhibition of free radical, reducing power and inhibition of lipid peroxidation. The antiproliferative activity of CTME with Cisplatin, anticancer drug was studied using human cervical cancer cells (HeLa). Proliferation of HeLa was measured by MTT assay, cell DNA content by modified diphenylamine method and apoptosis by Caspase 3 activity. The plant extract induced a marked concentration dependent inhibition on proliferation, reduced DNA content and apoptosis in HeLa. These results clearly indicate that C. tora is effective against free radical mediated diseases.     

HPLC法测定黎药山苦茶中没食子酸的含量

目的:建立以高效液相色谱法测定黎药山苦茶中没食子酸含量的方法。方法:色谱柱为C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸(5:95),检测波长为272nm,柱温为30℃,流速为1.0mL.min-1;以外标法计算样品含量。结果:没食子酸检测浓度在5～60μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9992);平均回收率为99.07%,RSD=2.80%(n=9)。结论:本方法简便、快捷,可用于山苦茶中没食子酸的含量测定。     

核桃楸皮抗肿瘤化学成分研究概述

核桃树皮具有消炎、止痛、抗氧化、抗肿瘤等作用,目前对核桃楸皮化学成分、药理作用的研究已取得一定的进展,尤其在其抗肿瘤方面的作用受到医药领域的广泛关注。对核桃树皮的抗肿瘤的化学成分的理化性质和药理作用作进一步的研究将有利于开发新的药物和充分利用天然药用资源。     

RP—HPLC法测定蒙药巴斯日陶克巴中没食子酸的含量

目的：建立采用RP—HPLC法测定巴斯日陶克巴中没食子酸的含量测定方法。方法：色谱柱为Diamonsil（钻石）C18（250ram×4．5mm,5斗）;流动相为甲醇一0．1％磷酸溶液（15：85）;流速lml·min～;检测波长273nm;外标法定量。结果：没食子酸进样量在0．04915μg-0．9831μg范围内呈良好的线性关系（r=0．99996）,加样回收率为98．07％,RSD为0．49％。结论：该方法简便、准确、可靠,可作为巴斯日陶克巴质量控制方法。     

HPLC测定玫瑰花中槲皮素、没食子酸的含量

目的：建立玫瑰花中槲皮素、没食子酸的含量测定HPLC方法。方法：Kromasil C18色谱柱（250mm×4．6mm,5μm）,槲皮素流动相为甲醇-0．4％磷酸溶液（53：47）,检测波长：360nm;没食子酸流动相为乙腈-0．1％磷酸溶液（3：97）,检测波长：274nm。结果：槲皮素浓度线性范围12．2～244．4μg·ml^-1,r=0．9999;没食子酸浓度线性范围5．6～112．2μg·ml^-1,r=0．9999。平均回收率为槲皮素99．27％,RSD=2．14％（n=6）;没食子酸98．68％,RSD=1．79％（n=6）。结论：方法简便、准确、重复性好,可用于该中药的含量测定。     

正交试验优选柿蒂鞣质的提取工艺

目的：优选柿蒂中鞣质类成分的提取工艺。方法：运用正交设计方案,采用磷钼钨酸-干酪素比色法,以没食子酸为对照考察了各种提取因素对提取率的影响,优化冷浸法和回流法的工艺条件。结果：两种提取方法中,回流提取效果较冷浸提取好。最佳提取工艺条件是溶剂为70％的丙酮,料液比为1：15,回流时间1．5h,回流温度40℃。柿蒂鞣质含量约为2．04％。结论：优化出的最佳提取工艺条件是合理的,鞣质成分提取率高。     

黄柏地榆胶囊的制备及质量控制

目的：研制黄柏地榆胶囊,制定质量标准。方法：采用TLC法对黄柏、地榆、黄芪、甘草进行定性分析;同时采用HPLC法测定地榆中没食子酸含量,色谱柱为Shim—pack—ODS柱（4．6mm×250mm,5μm）,流动相为乙腈-0．05mol·L^-1醋酸钠缓冲液（4：96,冰醋酸调pH至4．0）,流速为1．0mL·min^-1,检测波长为272nm。结果：TLC图谱斑点清晰,阴性对照无干扰;没食予酸在4．88～48．80μg·mL^-1范围内线性良好（r=0．9999）,平均回收率100．4％,RSD为1．67％（n=6）。结论：该方法适用于黄柏地榆胶囊的质量控制。     

野牡丹止痢片的质量标准

目的：建立野牡丹止痢片的质量标准。方法：采用TLC法对野牡丹进行鉴别;用HPLC法对野牡丹中没食子酸进行含量测定,色谱柱：Thermo BDS HYPERSIL C18（250mm×4．6mm,5μm）,流动相：甲醇-0．1％甲酸（5：95）,检测波长：272nm,流速：1．0ml·min^-1。结果：本品鉴别薄层色谱特征明显;没食子酸线性范围0．12～1．6μg（r=0．9999）,平均回收率为100．6％（RSD=1．3％,11=9）。结论：鉴别和含量测定方法准确可行,重复性好,可作为药品标准控制野牡丹止痢片的质量。