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31.
侯科佐 ' target='_blank'> 郑雪莹 ' target='_blank'> 包博文 ' target='_blank'> 卢文卿 ' target='_blank'> 金悦 ' target='_blank'> 王一喆 刘云鹏 ' target='_blank'> 车晓芳 ' target='_blank'> 《现代肿瘤医学》2020,(15):2639-2644
目的:探讨NFASC在胃癌中的预后意义及相关机制。方法:从人类肿瘤相关基因表达汇编（Gene Expression Omibus,GEO）数据库中下载GSE62254胃癌数据集,分析NFASC表达与胃癌临床病理学参数的相关性及对预后的影响;利用Cox模型分析影响胃癌患者总生存期的因素并建立预后预测列线图模型;采用基因集富集分析(GSEA)方法预测NFASC参与的信号通路。结果: NFASC高表达的胃癌患者总生存时间（overall survival,OS）更短（P<0.05）,NFASC表达与患者年龄、T分期及Lauren分型相关（P均<0.05）。NFASC高表达富集了TGF-β信号通路、mTOR信号通路、MAPK信号通路和Wnt信号通路等与肿瘤细胞增殖密切相关的通路（P<0.05,FDR<0.25）。结论: NFASC是胃癌的不良预后因素,其表达可能成为胃癌预后预测生物标志物。 相似文献
32.
目的:通过对癌症基因组图谱(The Cancer Genome Atlas,TCGA)、人类蛋白组图谱(The Human Protein Atlas,THPA)和人类肿瘤相关的基因表达汇编(Gene Expression Omnibus,GEO)数据库的数据挖掘,探索垂体肿瘤转化基因1（pituitary tumor-transforming gene 1,PTTG1）在乳腺癌中的表达及预后意义。方法:从TCGA、THPA和GEO数据库提取PTTG1在乳腺癌及正常组织中mRNA和蛋白水平的变化和患者临床病理资料,GEO在线预后分析数据库Kaplan-Meier Plotter(http://kmplot.com)检索PTTG1乳腺癌预后的相关性。结果:在数据库中,PTTG1 mRNA和蛋白水平在乳腺癌队列中表达显著高于正常乳腺组织(P<0.01);PTTG1 mRNA在不同的分子亚型中表达存在显著差异(P<0.05);PTTG1 mRNA在ER和PR阳性乳腺癌患者组织中表达显著低于ER和PR阴性乳腺癌患者(P<0.05);PTTG1 mRNA在进展期乳腺癌患者组织中表达显著高于早期乳腺癌患者(P<0.05);PTTG1 mRNA随着乳腺癌患者组织学分级程度升高而降低(P<0.05)。PTTG1低表达乳腺癌患者的预后显著好于其高表达患者(P<0.001);PTTG1高表达的ER和PR阳性、HER-2阴性乳腺癌患者的预后均显著差于其低表达者(P<0.05)。PTTG1高表达乳腺癌样本富集到细胞因子受体相互作用、趋化因子信号通路、细胞周期、自然杀伤细胞介导细胞毒性、Toll样受体信号通路和T细胞受体信号通路等相关基因集。结论:PTTG1 mRNA和蛋白在乳腺癌组织中高表达,PTTG1 mRNA表达与乳腺癌预后相关且在不同分子分型的乳腺癌中具有不同的预后监测意义,可以作为乳腺癌预后判断的标志物。 相似文献
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Chen Wang Jing Luo Jialing Rong Siying He Lei Zhang Fang Zheng 《Pathology, research and practice》2019,215(1):127-136
Background
The S100 protein family is implicated in tumor invasion and metastasis, but its prognostic roles in gastric cancer (GC) has not been elucidated.Materials and methods
In the current study, Kaplan-Meier plotter (KM plotter) database integrated the expression data and survival information of 1065 GC patients were downloaded from the Gene Expression Omnibus (GEO) (GSE22377, GSE14210 and GSE51105) that published by the three major cancer centers (Berlin, Bethesda and Melbourne). Then this database was used to explore the prognostic values of mRNA expression of each individual S100 in GC patients. We further assessed the prognostic value of S100 in different Lauren classifications, clinicopathological features and clinical treatment of gastric cancer.Results
Expression of 12 members of the S100 family correlated with overall survival (OS) for all GC patients. Increased expression of S100A3, S100A5, S100A7, S100A7A, S100A11, S100A13, S100Z and S100?G were found to be strongly associated with worse survival, while S100A8, S100A9, S100B and S100?P were correlated with better prognosis in all GC patients. Further assessment of prognostic values of S100 in gastric cancer with different clinical features indicated that different S100 members may interact with different signaling pathways and exerted different functions in gastric cancer development.Conclusions
Although the results should be further testified in clinical studies, our findings offer new insights into the contribution of S100 members to GC progression and might promote development of S100 targeted reagents for treating GC. 相似文献35.
Li Gao Li-jie Zhang Sheng-hua Li Li-li Wei Bin Luo Rong-quan He Shuang Xia 《Pathology, research and practice》2018,214(5):732-749
Background
MiR-452-5p has been reported to be down-regulated in prostate cancer, affecting the development of this type of cancer. However, the molecular mechanism of miR-452-5p in prostate cancer remains unclear. Therefore, we investigated the network of target genes of miR-452-5p in prostate cancer using bioinformatics analyses.Materials and methods
We first analyzed the expression profiles and prognostic value of miR-452-5p in prostate cancer tissues from a public database. Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), PANTHER pathway analyses, and a disease ontology (DG) analysis were performed to find the molecular functions of the target genes from GSE datasets and miRWalk. Finally, we validated hub genes from the protein–protein interaction (PPI) networks of the target genes in the Human Protein Atlas (HPA) database and Gene Expression Profiling Interactive Analysis (GEPIA). Narrowing down the optimal target genes was conducted by seeking the common parts of up-regulated genes from GEPIA, down-regulated genes from GSE datasets, and predicted genes in miRWalk.Results
Based on mining of GEO and ArrayExpress microarray chips and miRNA-Seq data in the TCGA database, which includes 1007 prostate cancer samples and 387 non-cancer samples, miR-452-5p is shown to be down-regulated in prostate cancer. GO, KEGG, and PANTHER pathway analyses suggested that the target genes might participate in important biological processes, such as transforming growth factor beta signaling and the positive regulation of brown fat cell differentiation and mesenchymal cell differentiation, as well as the Ras signaling pathway and pathways regulating the pluripotency of stem cells and arrhythmogenic right ventricular cardiomyopathy (ARVC). Nine genes—GABBR, PNISR, NTSR1, DOCK1, EREG, SFRP1, PTGS2, LEF1, and BMP2—were defined as hub genes in the PPI network. Three genes—FAM174B, SLC30A4, and SLIT1—were jointly shared by GEPIA, the GSE datasets, and miRWalk.Conclusions
Down-regulated miR-452-5p might play an essential role in the tumorigenesis of prostate cancer. 相似文献36.
目的 探索免疫治疗在恶性胸膜间皮瘤（malignant pleural mesothelioma,MPM）中免疫疗效相关关键分子及其可能的机制。方法 2018年7月至2020年7月,纳入GEO数据库中GSE117358的表达谱进行分析;首先,根据是否对免疫治疗是否有效分为两组：免疫治疗反应组和免疫治疗无反应组,同时分析两组之间差异有统计学意义基因;其次,分析两组差异有统计学意义基因在基因本体（gene ontology,GO）生物学行为及京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes,KEGG）富集信号通路等方面的差异;最后,对差异有统计学意义基因构建蛋白-蛋白互作网络,筛选在生物学行为中的重要模块及关键基因,并对关键基因在TCGA数据库中进行整合分析验证。结果 在免疫治疗反应组（12个样本）及免疫治疗无反应组（12个样本）中共筛选出1 025个差异基因,相对于免疫治疗无反应组,在免疫治疗反应组中有782个上调和243个下调基因。GO和KEGG分析显示,这些差异基因的功能主要集中在细胞因子受体通路、细胞黏附通路、T细胞受体通路及... 相似文献
37.
目的:探究作用于月经增殖期及分泌期子宫内内膜异位症的共享潜在致病基因。方法:利用两个原始数据集GSE7305、GSE25628,筛选出18例子宫内膜异位症患者在位子宫内膜与匹配的异位子宫内膜组织相比的差异表达基因(DEGs),并使用基因本体论分析,KEGG(Kyoto Encyclopedia of Genes and Genomes)分析,利用STRING 11.0建立蛋白质相互作用网络。利用Cytoscape MCODE软件筛选热点模块并使用BiNGO进行相关基因的GO分析。结果:两个数据集共享96个DEGs。基因本体论分析显示DEGs富集于补体激活等通路。CLU等基因可能介导补体激活参与子宫内膜异位症的发生发展。结论:CLU等基因可能通过参与补体激活参与子宫内膜异位症的发生发展。 相似文献
38.
目的研究IGFBP5基因在结直肠癌中的表达水平及临床意义,探讨IGFBP5在结直肠癌发生发展中的作用及其分子机制。方法使用RT-qPCR实验检测IGFBP5在结直肠癌组织和癌旁组织中的表达水平;分别从GEPIA2和Linked Omics数据库分析IGFBP5在结直肠癌组织中的表达与临床病理参数之间的相关性和对预后的影响;使用Linked Omics数据库导出结直肠癌中与IGFBP5相关的前1000位相关基因,并使用DAVID网站分析IGFBP5的分子功能和相关信号通路;使用STRING网站和Cytoscape软件预测与其可能与IGFBP5发生相互作用的蛋白网络。结果RT-qPCR结果显示:IGFBP5基因在结直肠癌组织中表达水平显著性升高(P<0.05);IGFBP5表达水平与结直肠癌N分期呈正相关(P<0.05),而与结直肠癌病理分级、T分期和M分期均无显著相关性(P均>0.05);IGFBP5表达水平越高,患者的总生存期和无病生存期越差(P均<0.05);GO分析结果显示:IGFBP5在结直肠癌中与细胞黏附、血管生成和血管内皮生长因子等多种促癌功能有关(P均<0.05);KEGG分析结果显示:IGFBP5基因与PI3K-Akt信号通路激活有关(P均<0.05);蛋白互作网络图结果显示:在结直肠癌中IGF1、IGF2、SPP1、LTBP1和FAM20C基因与IGFBP5基因关联最紧密。结论IGFBP5基因在结直肠癌组织中表达水平升高,并且IGFBP5的表达与结直肠癌淋巴结转移正相关,与临床预后负相关,因此IGFBP5可作为结直肠癌初步诊断和判断预后的标志物。 相似文献
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40.
Chang-Kyu Oh Mihyang Ha Myoung-Eun Han Hye J. Heo Kyungjae Myung Yoonsung Lee Sae-Ock Oh Yun Hak Kim 《Hematological oncology》2020,38(3):381-389
Accurate prediction of malignancies is important in choosing therapeutic strategies. Although there are many genetic and cytogenetic prognostic factors for acute myeloid leukemia (AML), prognosis is difficult to predict because of the heterogeneity of AML. Prognostic factors, including messenger RNA (mRNA) expression, have been determined for other malignancies, but not for AML. A total of 402 patients from The Cancer Genome Atlas, GSE12417 (GPL96, 97), and GSE12417 (GPL570) were included in this study. In Kaplan-Meier curve analyses, high expression of family with sequence similarity 213 member A (FAM213A), which activates antioxidant proteins, was associated with worse prognosis of AML. Similar to the results of the survival curve, C-indices and area under the curve values were high. Current prognostic factors of AML, unlike those of other cancers, do not take mRNA expression into consideration. Thus, the development of mRNA-based prognostic factors would be beneficial for accurate prediction of the survival of AML patients. Additionally, in vivo validation using zebrafish revealed that fam213a is important for myelopoiesis at the developmental stage and is a negative regulator of the p53 tumor suppressor gene. The findings implicate fam213a as a novel prognostic factor for AML patients. 相似文献