中药苦参提取方法的比较和工艺条件优化

目的优选苦参中氧化苦参碱的提取工艺。方法比较多种提取工艺。如水煎法、渗滤法、乙醇回流法,应用正交设计筛选苦参碱的提取工艺,采用高效液相色谱法测定该成分含量,作为评价指标。结果最佳工艺为渗滤法。最佳条件是:加10倍量65%乙醇浸泡24 h后渗滤,流速为5 m l/m in,氧化苦参碱含量与提取率均较高。结论优选得到的工艺简便易行,稳定性好。     

高效液相色谱法测定菊花中绿原酸含量的方法改进

目的对菊花中绿原酸含量的测定方法进行改进。方法采用高效液相色谱(HPLC)法测定绿原酸含量。结果菊花中绿原酸的含量按《中国药典》2005年版Ⅰ部方法实验,样品提取方法复杂,含量有损失,误差较大。结论将含量测定方法改进后,具有样品溶液提取方法简便、含量准确可靠、分离效果好、灵敏度高、稳定性好等优点,为标准的修订提供可行的依据。     

金银花产量和质量的物候学分析

不同物候期采收的金银花产量和质量存在显著差异。第一茬花产量最高,其后几茬花产量逐次降低;第一茬花千蕾重及绿原酸含量最高,第四茬花次之,第二、三茬花较低。这种差异主要与不同物候期间花芽分化历时、花枝的类型、叶面积、叶绿素含量等生物学因素有关,不同物候期的气温、日照时数、降水量等气候因子也密切影响药材质量。     

不同广豆根炮制品中苦参碱含量比较

目的：探讨不同炮制方法对广豆根中苦参碱含量的影响。方法：采用双波长薄层扫描法对不同广豆根炮制品中苦参碱进行测定。结果：广豆根不同的炮制品与生品中苦参碱比较有显著性差异。结论：不同炮制方法、辅料使广豆根炮制前后苦参碱含量发生变化。     

毛细管气相色谱法测定金银花中敌敌畏、甲基对硫磷和马拉硫磷的残留量

目的：建立测定金银花中敌敌畏、甲基对硫磷和马拉硫磷残留量的毛细管气相色谱分析方法。方法：样品以微波提取的方法进行处理。以FPD为检测器,色谱柱为HP-5石英毛细管柱（25m×0.32mm,0.17μm）,载气为高纯氮气,流速20ml/min;氢气流速45ml/min;空气流速450ml/min;程序升温：初始温度为120℃,最终温度为230℃,升温速率10℃/min。进样口温度250℃,检测器温度260℃。采用无分流进样方式。结果：敌敌畏、甲基对硫磷和马拉硫磷的测定浓度在0.25￣3.00μg/ml时,浓度与峰面积呈线性关系。当信噪比为2时,敌敌畏的最低检出量为0.3ng,甲基对硫磷的最低检出量为0.15ng,马拉硫磷的最低检出量为0.2ng。敌敌畏的平均回收率为88.7%,RSD为2.1%;甲基对硫磷平均回收率为89.7%,RSD为1.7%,马拉硫磷平均回收率为90.6%,RSD为1.1%。结论：该方法操作简便、准确、可靠,可用于金银花中敌敌畏、甲基对硫磷和马拉硫磷残留量的测定。     

白山片的薄层色谱鉴别方法研究

目的建立白山片中白鲜皮、山豆根、北豆根的鉴别方法。方法采用薄层色谱法对白山片处方中主要的3味中药白鲜皮、山豆根、北豆根进行定性鉴别。结果各供试品色谱中，在与对照色谱相应的位置上显相同颜色的斑点。色谱清晰、无杂质斑点干扰。结论本文所制定的方法专属性强，重复性好，可有效地控制白山片的质量。     

金銀花提取工艺研究

目的 研究金银花的最佳提取条件.方法 采用正交试验法以乙醇浓度(A)、乙醇用量(B)、回流时间(C)、回流次数(D)4个因素,每个因素选取3个水平进行试验.结果 因素C对结果 影响显著,因素A次之,因素B影响最小.结论 最佳提取工艺为以10倍量40%的乙醇为提取溶剂,80℃提取2次,每次1.5 h.     

复方金银花冲剂的质量分析

采用薄层层析、薄层色谱法对复方金银花冲剂中的主药金银花、连翘进行了定性定量分析。检测方法较简便、专属性强，可作为该冲剂的质量控制指标。     

Effects of a Korean herbal formulation, Silsosangami, consisting of seven medicinal herbs, and its seven herbs on endotoxin-induced experimental thrombosis in rats

A traditional Korean medicine, Silsosangami (SSG), consisting of seven different herbs of Typhae Pollen, Pteropi Faeces, Paeoniae Radicis rubra, Cnidii Rhizoma, Persicae Semen, Carthami Flos and Curcumae Tuber, has been reported to have a hypolipidemic effect in human subjects. In the present study, the inhibitory effects of SSG on a thrombosis in rats, induced by endotoxin treatment were examined. The anti-thrombic properties of SSG were also investigated with respect to blood parameters. The extracts of SSG and five of the seven herbs, except Cnidii Rhizoma and Carthami Flos, inhibited both endotoxin-induced disseminated intravascular coagulation (DIC) and thrombosis in rats. The extract also inhibited the endotoxin-induced decrease in blood platelets and fibrinogen, and the endotoxin-induced increase in fibrin degradation products (FDP) on disseminated intravascular coagulation in normal rats. In conclusion, the artificially induced, protective effects of SSG on ischemic infarction might be related to their inhibitory effects on DIC, platelet coagulation and thrombotic action.     

High performance liquid chromatographic fingerprint evaluation of the quinolizidine alkaloids from commercial Radix Sophorae Flavescentis

Fifteen commercial samples of Radix Sophorae Flavescentis were collected from different parts of China. HPLC analysis showed that the commercial samples all contained quinolizidine alkaloids and a total of nine chromatographic peaks were identified by referring to standard compounds, HPLC/MS analysis and comparison of the physicochemical data of the isolated peak with the literature. The contents of the major alkaloids were determined and the ratio of the major alkaloids contents was shown to be correlated with their source of origin. The commercial samples from China gave a distinct HPLC pattern showing the main alkaloids contents. The sample preparations were researched and the extraction conditions of the alkaloids were optimized. Reproducible HPLC fingerprints can be obtained for the quinolizidine alkaloids under the well-controlled extraction conditions. The HPLC fingerprint analysis method is suitable for the quality control of the Radix Sophorae Flavescentis and the standardization of phytomedicines.