Two-step tumour targetting in ovarian cancer patients using biotinylated monoclonal antibodies and radioactive streptavidin

A new method for intraperitoneal tumour targetting in ovarian cancer using biotinylated monoclonal antibodies (MoAb) and radioactive streptavidin is described. Fifteen patients with histologically documented ovarian carcinoma were injected intraperitoneally with 2 mg of biotinylated MoAb MOv18, followed 3–5 days later by 100–150 g of indium-111 streptavidin, at the specific activity of 280–370 MBq/mg in 500 ml of normal saline. No toxicity was observed. Tumours were imaged from 2 to 48 h after radioactivity injection by recording both planar and single photon emission tomography (SPET) data. All patients underwent surgery 1–8 days later (mean 3 days) after scanning. The resected tumour and normal tissue radioactivity were measured. On the day of surgery, the tumour to normal tissue ratio was 9:1 (range 3:1–30:1) and 45:1 (range 12:1–120:1) for intra- and extraperitoneal samples, respectively. The mean tumor to blood ratio was 14:1 (range 4:1–30:1). The injected dose (i.d.) per gram of tumour was 0.112 (range 0.01–0.3) for recurrences and 0.05 for primary tumour (range 0.005–0.2). Over 24–48 h 14% i.d. (range 8–18% i.d.) was found in the urine, 14% i.d. (range 629% i.d.) in the blood and 63% i.d. (range 56–70% i.d.) was still in the peritoneal cavity. These preliminary clinical data suggest that this two-step strategy may be superior to the conventional approach (radiolabelled antibodies) for intraperitoneal radioimmunolocalization and radioimmunotherapy of ovarian cancer. Offprint requests to: G. Paganelli     

Innervation of Entorhinal Principal Cells by Neurons of the Nucleus Reuniens Thalami. Anterograde PHA-L Tracing Combined with Retrograde Fluorescent Tracing and Intracellular Injection with Lucifer Yellow in the Rat

The innervation of dendrites of identified entorhinal principal cells by fibres originating in the nucleus reuniens thalami was studied in the rat. The lectin Phaseolus vulgaris-leucoagglutinin (PHA-L, anterograde tracer) was injected into the nucleus reuniens and the fluorescent dye Fast Blue (retrograde tracer) into the hippocampus. After survival, perfusion-fixation and the preparation of brain slices, entorhinal neurons retrogradely labelled with Fast Blue were intracellularly injected with the dye Lucifer yellow to introduce a specific marker into their dendrites. The transported PHA-L and the injected Lucifer yellow were visualized through dual peroxidase immunohistochemistry. Varicosities on PHA-L labelled reuniens fibres abut ascending and descending Lucifer yellow-filled secondary dendrites of multipolar and pyramidal principal entorhinal neurons that possess either spiny or sparsely spiny dendrites, but they do not appose the perikarya of these cells. In the electron microscope, PHA-L labelled boutons in the entorhinal cortex were observed forming asymmetrical synaptic contacts with dendritic spines (50%) or shafts (50%). The results indicate that direct thalamic input occurs on dendrites of neurons in the entorhinal cortex which project to the hippocampus.     

不同浓度依达拉酮注射液的细菌内毒素检测方法

目的:建立依达拉酮注射液的细菌内毒素检查方法。方法:用凝胶法比较不同浓度的依达拉酮注射液对细菌内毒素检测的干扰情况。结果:采用灵敏度为0.125EU·mL~(-1)的鲎试剂,将本品稀释后,浓度小于或等于0.0375g·L~(-1)时对鲎试剂与内毒素的凝集反应无干扰。结论:本品可用细菌内毒素检查法代替热原检查来控制其质量,其细菌内毒素限期值可定为3.33EU·mg~(-1)。     

罗通定中降解物巴马汀限量检测方法

目的：研究解决罗通定及硫酸罗通定注射液中降解物巴马汀的检测方法。方法：用薄层色谱法和紫外分光光度法限量检查样品中的巴马汀。结果：与对照品斑点大小、荧光强度比较和用吸收度限值控制均可对样品中的巴马汀进行限量检查，结果与原标准一致。结论：两方法均灵敏，专属，简便，快速。     

注射用盐酸丁咯地尔细菌内毒素检查法的研究

研究注射用盐酸丁咯地尔对细菌内毒素检查试验的干扰情况 ,并建立其细菌内毒素检查的方法。按《中国药典》 2 0 0 0年版二部附录ⅪE细菌内毒素检查法和附录ⅪⅩF细菌内毒素检查法应用指导原则进行试验。本品在 0 33mg·ml-1稀释浓度下无干扰作用 ,L =1 5EU·mg-1。本品可用细菌内毒素检查法替代热原检查法     

喜炎平和利巴韦林治疗急性上呼吸道感染的比较

目的 比较喜类平注射液(主要成份:穿心莲内酯)和利巴韦林注射液治疗急性上呼吸道感染的临床疗效。方法 将204例急性上呼吸道感染患儿随机分成两组,治疗组133例,给予喜炎平注射液0.2～0.4m/(kg·d)。对照组71例,给予利巴韦林注射液10～15mg/(kg·d),两组均加苯唑青霉素50mg/kg·d,静滴。结果 治疗组治愈时间5.14±1.50d,对照组5.55±1.46d,两组平均治愈时间无显著差异(P>0.05),但治疗组平均退热时间与对照组比较有显著差异(P<0.05)。结论 喜炎平注射液具有抗病毒,抗菌作用,副作用小,是治疗小儿急性上呼吸道感染较理想的药物。     

参麦注射液对培养大鼠主动脉平滑肌细胞增殖的影响和机理研究

目的 研究参麦注射液对血管平滑肌细胞增殖的影响和机理。方法 以培养的大鼠主动脉血管平滑肌细胞为模型，通过细胞计数及测量3H-胸腺嘧啶核苷掺入量观察细胞增殖情况，同时测定培养液中超氧化物歧化酶(SOD)活性、脂质过氧化物、前列环素(PGI2)的含量，比较分析加入不同剂量参麦注射液后上述指标的变化和相互关系。结果 不同剂量参麦注射液组的平滑肌细胞增殖及3H—胸腺嘧啶核苷掺入量均显著低于对照组，SOD活性及PGI2含量显著高于对照组，脂质过氧化程度显著降低。结论 参麦注射液对血管平滑肌细胞增殖具有抑制作用，并呈剂量依赖关系，其作用可能与增加SOD活性、降低脂质过氧化物水平，升高PGI2／TXA2有关。     

比色法测定消癌平注射液中总酚酸的含量

目的　建立消癌平注射液中总酚酸的含量测定方法。方法　以绿原酸为对照品 ,比色法测定。结果　绿原酸回归方程为A =35 8.1 4×C - 5 .3879(r=0 .9993,n =9)。在 2 0 .5～ 32 8μg范围内呈线性关系 ,平均回收率为 1 0 0 .4 8% ,RSD =1 .70 %。结论　所用方法简便、准确、快速 ,可作为该制剂的定量方法     

参麦注射液对内皮细胞增殖和迁移的影响

目的：探讨参麦注射液对血管生成的影响。方法：采用MTT法检测参麦注射液对牛血清和肿瘤细胞条件培养液促进的牛主动脉内皮细胞增殖的影响，采用琼脂糖刮除法检测参麦对牛血清和肿瘤条件培养液促进的牛内皮细胞迁移的影响。结果：在含10％新生小牛血清培养液中和在肿瘤细胞条件培养液中，参麦都能明显抑制牛主动脉内皮细胞增殖，且呈量效关系，对肿瘤细胞条件培养液诱导的内皮细胞迁移抑制作用显著。方法：参麦能抑制牛内细胞增殖和迁移，具有抑制血管生成的作用。     

康莱特联合化学药物动脉灌注治疗转移性肝癌的临床研究

目的：观察康莱特联合化学药物动脉灌注治疗转移性肝癌的疗效。方法：152例转移性肝癌患者随机分为治疗组(康莱特联合化学药物动脉灌注)79例、对照组(化学药物动脉灌注)73例。结果：治疗组的稳定率为87．3％，1年生存率为73．4％，2年生存率为50．6％，均明显高于对照组；并可减低血液高凝状态，提高患者的细胞免疫功能，缓解化疗药物的毒副反应，改善患者的生活质量。结论：康莱特联合化学药物动脉灌注治疗转移性肝癌有较好的疗效。