Strips of rat mesenteric artery were loaded with carboxy-seminaphthorhodafluor (SNARF) to measure intracellular pH (pHi) and force simultaneously. pHi was altered by using weak acids and bases. Alkalinization produced an increase in force. For equal elevations of pHi a greater and faster increase of force was obtained in depolarized (high K+) than in non-depolarised preparations. Acidification produced little change in force unless the tissue was contracted (high-K+), in which case it elicited relaxation. Examination of the relationship between pHi and force in depolarized preparations showed that acidification produced a greater change in force than alkalinization. Removal
of weak bases produced a transient acidification that was accompanied by a fall in force in all preparations. This was followed
by a secondary contraction in depolarized preparations during the period over which pHi was acidic and being restored to resting values. Some preparations demonstrated a hysteresis in the relation between pHi and force. It is concluded that the relationship between pHi and force in mesenteric vascular smooth muscle is not constant but depends on the previous history of the preparation, and
may involve differences in the interactions between H+, Ca2+ and the contractile machinery. 相似文献
Summary Many articles have been written about wound contraction. The majority of these use animals as these experiments are impossible to repeat in humans for ethical reasons. Skin graft donor sites have been used in this study as repeatable controlled wounds. Wound contraction does occur in these wounds and it can be reduced by covering the wound with a synthetic dressing. 相似文献
The effects of prostaglandin E2 (0.2 μM) and indomethacin (50 μM) on the rebound of smooth muscle cells of the guinea-pig taenia coli were studied. Stimulation of the non-cholinergic, non-adrenergic, intramural nerves caused membrane hyperpolarizaiton, known as the inhibitory junction potential (I.J.P.). This hyperpolarization was followed by a rebound depolarization and a rather small rebound contraction in quiescent preparations; the rebound depolarization was often accompanied by action potentials, resulting in a pronounced rebound contration. The effects of prostaglandin E2 (PGE2) on the membrane were comparable with the phenomena observed during the rebound, i.e. membrane depolarization, development of action potentials and contraction of the smooth muscle cells. Furthermore, an increase in amplitude of the I.J.P., enhancement of the spike discharge and a concomitant increase in rebound contraction were observed in the presence of PGE2. Indomethacin did not modify the membrane potential or the amplitude of the I.J.P., but inhibited the rebound contraction and suppressed the development of action potentials during the rebound. The action of PGE2 on the smooth muscle cell membrane was not modified by indomethacin, but the rebound contraction in the presence of both compounds was decreased. These experimental results indicate: that the rebound contraction is accompanied by depolarization of the membrane following the hyperpolarization caused by stimulation of the non-cholinergic non-adrenergic nerves; that the rebound activity can be mimicked by PGE2; that indomethacin does not interfere with the action of PGE2 applied exogenously and that the observations are consistent with the assumption that prostaglandins might be involved in the rebound. 相似文献
Adenomyosis (AM) has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells (MSMCs) of AM.
Methods:
Uterine tissue samples from 22 patients (cases) with histologically confirmed AM and 12 (controls) with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium- and voltage-sensitive K+ channel (BKCa)-α/β subunits, voltage-gated potassium channel (Kv) 4.2, and Kv4.3. Student''s t-test was used to compare the expression.
Results:
The BKCa-α/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-α messenger RNA (mRNA) (0.62 ± 0.19-fold decrease, P < 0.05) and Kv4.3 mRNA (0.67 ± 0.20-fold decrease, P < 0.05) decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA.
Conclusions:
The BKCa-α mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain. 相似文献
To clarify whether or not reperfusion injury occurs in the permanent occlusion of a coronary artery, we analyzed quantitatively contraction band necrosis as an indicator of early recanalization, coagulation necrosis, infarct size and measured regional blood flow in dog hearts with collateral circulation. Fifty mongrel dogs were divided into four groups: 15 dogs with a 24-hour occlusion of the left anterior descending coronary artery just distal to the first diagonal branch (permanent occlusion group): 15 dogs a with 3-hour occlusion followed by 24-hour recanalization (recanalization group); 10 dogs with a 2-hour occlusion without recanalization (transient occlusion group); 10 dogs with a 4-hour occlusion without recanalization (transient occlusion group).
The regional blood flow in the subepicardium and subendocardium determined by the generated hydrogen gas clearance method was greatly decreased 30 minutes after occlusion (14 + 8%/12 ± 9%) and was relatively restored from 180 minutes (31 ± 21%/21 ± 14%) to 24 hours later (41 + 19%/26 + 16%) in spite of complete occlusion of the coronary artery. The percentage infarct area in the risk area was significantly greater in the permanent occlusion group (60 ± 26%) than in the recanalization group (35 ± 31%). Although most of the infarct was occupied by contraction band necrosis in the recanalization group (86 ± 12%), contraction band necrosis was diffusely seen even in the permanent occlusion group (54 ± 27%). In both the permanent and recanalization groups, contraction band necrosis was the main histological feature of small infarcts occupying less than 30% of the risk area, while coagulation necrosis was the main feature in very large infarcts occupying more than 80% of the risk area. In the occlusion groups without recanalization, the percentage area of contraction band necrosis in the risk area was 6 ± 8% after the 2-hour occlusion, 23 ± 17% after the 4-hour occlusion and 31 ± 21% after permanent occlusion; the difference between the 4-hour and permanent occlusion groups was not significant. In the permanent occlusion group, the percentage infarct area in the risk area was inversely correlated with regional blood flow during occlusion, an indicator of collateral flow. It was concluded that reperfusion injury occurs even in hearts without recanalization. The pathogenesis may involve reperfusion in the risk area via collateral circulation. Protection against reperfusion injury is important to minimize the infarct size even in hearts with permanent occlusion, although the presence of collateral flow is an important factor in limiting infarct size. 相似文献
ObjectiveTo investigate whether clinical tests used to detect motor control dysfunction can predict improvements in pain and disability in patients with chronic nonspecific low back pain (LBP) who have undergone an 8-week lumbar stabilization exercise program.Study DesignA prospective cohort study.SettingOutpatient physical therapy university clinic.ParticipantsSeventy people with chronic nonspecific LBP were recruited, and 64 completed the exercise program (N=64).InterventionsThe lumbar stabilization program was provided twice a week for 8 weeks.Main Outcome MeasuresPain intensity (11-point numerical rating scale) and disability (Roland Morris Disability Questionnaire) and clinical tests, such as the Deep Muscle Contraction (DMC) scale, Clinical Test of Thoracolumbar Dissociation (CTTD), and Passive Lumbar Extension (PLE) test. Univariate and multivariate linear regression models were used in the prediction analysis.ResultsMean changes in pain intensity and disability following the 8-week stabilization program were ?3.8 (95% confidence interval [CI], ?3.2 to ?4.4) and ?7.4 (95% CI, ?6.3 to ?8.5), respectively. Clinical test scores taken at baseline did not predict changes in pain and disability at 8-week follow-up.ConclusionOur findings revealed that the DMC scale, CTTD, PLE test, clinical tests used to assess motor control dysfunction, do not predict improvements in pain and disability in patients with chronic nonspecific LBP following an 8-week lumbar stabilization exercise program. 相似文献