咳尔康口服液体外抑菌作用的研究

Objective To analyze the effect of Kecrkang oral Liquid on extracorporeal bacteriostasis. Methods Tube dilution method was adopted to test the effect of different concentrations of Keerkang oral liquid on bacillus coli, klebsiella pneumoniae, bacillus aeruginosus, staphylococcus aureus, staphylococcus epidermidis, β-Streptococcus hemolyticus, streptococcus pneumoniae, haemophilus influenzae and candida albicans. Results Keerkang Oral Liquid has obvious inhibited effects to all the bacteria that mentioned above. Conclusion Keerkang Oral Liquid has obvious extracorporeal bacteriostasis and resistance to fungi.     

镫骨全切除和吸引噪声对豚鼠耳蜗功能与超微结构的影响

本实验应用耳蜗电图和扫描、透射电镜等技术观察了6组(44只)豚鼠镫骨全切除后,前庭窗开放时间的长短和听泡内使用吸引器对耳蜗功能和超微结构的影响程度.结果如下:①随前庭窗开放时间的延长、复合蜗神经动作电位(compound action potential.CAP)反应阈进行性提高,开放180min可导致底回部分外毛细胞变性或破坏;②镫骨全切除后,听泡内较长时间地(60min)应用吸引器可引起CAP明显阈移和耳蜗底回部分外毛细胞的不可逆病变;③镫骨未切除时,听泡内吸引60min,仅引起CAP阈移,但外毛细胞未见损伤.结果提示:术中长时间开放前庭窗和鼓室内应用吸引器可能是引起镫骨全切除术后感音神经性聋的因素之一,但鼓室成形术中鼓室内吸引噪声可能因其强度不够大,不足以引起术后感音神经性聋.     

HPLC法测定清热解毒口服液黄芩苷含量

目的：测定清热解毒口服液中黄芩苷的含量。方法：采用HPLC法，用C18柱，甲醇-水-磷酸（50：50：0．3）为流动相，检测波长276nm。结果：在4．775～191μg/ml的浓度范围具有良好的线形关系。R=1．000（n=6），平均回收率105．6％，日内精密度为1．63％（n=5）。结论：此方法快速、专属，准确。     

复方七芍降压片治疗高血压病的临床研究

目的:探讨复方七芍降压片(三七,白芍,天麻等)治疗高血压病的临床疗效及作用机制.方法:采用随机单盲对照法设置治疗组与对照组,治疗组服用复方七芍降压片,对照组服用卡托普利.观察两组治疗前后血浆神经肽Y(NPY)的变化及血小板聚集率的变化.结果:两组均能降低血压及血浆NPY的含量,但无统计学意义;治疗组中医证候总有效率优于对照组;治疗组可降低血浆FIB的含量及改善血小板聚集率,治疗前后自身比较有统计学意义,而对照组则不显著.结论:复方七芍降压片对高血压病有较好的临床疗效且无明显毒副反应.     

Effects of etomidate on free intracellular amino acid concentrations in polymorphonuclear leucocytes in vitro

BACKGROUND: Previous studies have shown the inhibitory effects of etomidate on polymorphonuclear leucocyte (PMN) function. No reports exist, however, regarding free intracellular amino acid metabolism, although physiological cell metabolism and basic cell functions rely upon a balanced intracellular amino acid content and the cell membrane-mediated separation of cellular amino acids from the extracellular plasma amino acid pool. Thus, in the current study, we evaluated the effects of etomidate on free intracellular amino acid metabolism in PMN. METHODS: With ethics committee approval, blood was withdrawn from 35 healthy volunteers and incubated (1 h) either with 0 microg/ml, 0.0156 microg/ml, 0.0625 microg/ml or 0.5 microg/ml of etomidate as well as with its additives (propylene glycol and Lipofundin MCT 10%). The PMN were separated using standardized Percoll-gradient and centrifugation procedure before deep-freezing and lyophilization techniques were employed. All PMN samples were dissolved in methanol/H2O, and the concentrations of free intracellular amino acids were monitored using both novel advanced PMN-separation and high-performance liquid chromatography techniques. RESULTS: Etomidate influenced important free amino acid profiles in PMN in a dose-dependent manner, indicating complex changes of cellular amino acid turnover. Neither propylene glycol nor Lipofundin MCT 10% changed free amino acid concentrations in PMN. CONCLUSIONS: For the first time, the effects of etomidate on free intracellular amino acid metabolism in PMN have been investigated. Our results draw attention to the biochemical pathways which may be involved in etomidate-induced alterations in PMN function and cellular immunocompetence.     

稳态强噪声暴露后听神经动作电位及神经丝蛋白表达的观察

目的 观察豚鼠暴露于稳态强噪声后听神经复合动作电位(CAP)及神经丝蛋白(NFP)的变化。方法 选用20只(40耳)Preyer反射正常的健康豚鼠，分为4组，噪声暴露后存活7天组(N-7d)、14天组(N-14d)和2个月组(N-2m)各5只，空白对照组(C)5只。噪声强度130dB(A)SPL，连续暴露5小时，于暴露前、暴露后3、7、14天、暴露后2个月分别测试CAP—N1波反应阈。免疫组化反应分别检测对照组、暴露后7、14天组、暴露后2个月组动物耳蜗内NFP的表达。结果稳态强噪声暴露后CAP—N1波反应阈明显增高，于暴露后7天基本稳定。暴露后7、14天NFP表达无明显变化，暴露后2月NFP表达显著减弱。结论 130dB(A)SPL稳态强噪声暴露5小时可以导致豚鼠CAP中重度永久性阈移，噪声损伤的早期NFP改变不明显，耳蜗NFP染色反应随着存活时间延长逐渐减弱，反映了损伤后神经纤维中骨架蛋白存在变性过程。     

应用高效液相色谱法检测男性不育患者精浆丙二醛水平

目的:探讨高效液相色谱法(HPLC)检测精浆中脂质过氧化产物丙二醛(MDA)水平的意义。方法:93例不育男性分为:阻塞性无精子症组(12例);非阻塞性无精子症组(15例);少精子症组(21例);弱精子症组(19例);少弱精子症组(16例);少弱畸精子症组(10例)。18例生育男性作为对照组。采用HPLC检测生育与不育男性精浆MDA含量。结果:生育组精浆MDA值除与阻塞性无精子症组差异无显著性(P>0.05)外,与其他各组相比均有极显著性差异(P均<0.01)。各不育男性组间精浆MDA值也有统计学差异。结论:应用HPLC检测精浆MDA水平是诊断活性氧产生过高所致男性不育的一个重要指标。     

反相高效液相色谱法测定托拉噻米胶囊中托拉噻米的含量

目的建立高效液相色谱法测定托拉噻米胶囊中托拉噻米的含量。方法以岛津Sh impack CLC(M)C18(250 mm×4.6 mm,5μm)为色谱柱,以甲醇-0.02 mol.L-1磷酸二氢钾溶液(用磷酸调节pH至3.0)(65∶35)为流动相,检测波长为UV291 nm,流速为0.9 ml.min-1。结果托拉噻米在2~24μg.ml-1范围内具有良好线性关系(r=0.999 9),平均加样回收率为99.73%,相对标准差为0.17%;三批样品的标示百分含量(%)分别为99.8、100.1及100.4。结论本方法准确、灵敏度高、重现性好,可用于托拉噻米胶囊的含量测定。     

人血浆中头孢氨苄浓度高效液相色谱法测定

目的建立高效液相色谱法测定人血浆中头孢氨苄的浓度。方法采用噻克硝唑为内标,色谱柱为YMC ODS-A C18(150 mm×4.6 mm),流动相为乙腈∶甲醇∶pH 4.5的醋酸盐缓冲液(6∶10∶84),流速为1.0 mL/min,检测波长为260 nm。结果头孢氨苄血清浓度在0.101~32.100μg/mL范围内具有良好线性关系,最低定量限为0.101μg/mL,方法回收率为97.5%~103.0%,日内、日间相对标准差均<7%。结论高效液相色谱法简便、准确、灵敏,适用于头孢氨苄血药浓度监测和药代动力学研究。     

Effects of perfluorochemical evaporative properties on oxygenation during partial liquid ventilation

BACKGROUND: The physical-chemical properties of perfluorochemical (PFC) liquids have been shown to influence physiological and cellular responses during partial liquid ventilation (PLV). The aim of this study is to compare the relationship between patho-physiological endpoints and the physical properties of three PFC liquids used in treating acute lung injury. METHODS: A total of 18 juvenile rabbits were randomized into conventional mechanical ventilation or PLV groups after lung saline lavages. Three PFC liquids, including Flutec perfluoro-1,3,5-trimethylcyclohexane (PP4; vapor pressure, 28.8 mmHg at 37 degrees C), Perfluorodecalin (PFD; vapor pressure, 13.6 mmHg at 37 degrees C), and Perflubron (PFB; vapor pressure, 10.4 mmHg at 37 degrees C) were used for PLV with no replacement for 4 h. A thermal detector was used to measure PFC loss rate. Physiological measurements and evaporative loss rate of PFC were done every 30 min, and lung histology was examined. RESULTS: The mean evaporative loss rate was significantly higher in the PP4 group (4.75 +/- 0.24 mL/kg per h) than in either the PFD (1.43 +/- 0.11 mL/kg per h) or the PFB (1.18 +/- 0.05 mL/kg per h) group (P < 0.05). The oxygenation of PFD and PFB was maintained good for 4 h, however, the PP4 group showed a fast deterioration since 2 h post-treatment due to fast dropping of the residual PP4 amount in lungs. Histology showed good alveolar integrity in the PFD and PFB groups. CONCLUSIONS: The effects of PLV are directly influenced by the evaporative property of the PFC liquid. With no replacement over 4 h, PLV effects could be maintained with utilizing a PFC liquid with low, rather than high, vapor pressure. PFC with high vapor pressure has a high loss rate and low residual volume that causes poor maintenance on oxygenation during PLV. Therefore, measuring PFC loss rate is important in future studies and clinical application of PLV.