早发性脊柱侧凸手术中神经功能监测的应用及意义探讨

目的探讨术中经颅电刺激运动诱发电位(transcranial electric Motor Evoked Potential,TceMEP)和体感诱发电位(somatosensory evoked potentials,SSEP)监测在早发性脊柱侧凸(early-onset scoliosis,EOS)脊柱截骨矫形手术中的应用价值,并分析此类手术中的神经电生理监测特点。方法收集2015年9月至2016年12月由北京协和医院骨科收治的20例EOS和120例特发性脊柱侧凸(adolescent idiopathic scoliosis,AIS)患者进行回顾性研究,其中20例EOS患者平均年龄(2.65±0.11)岁,120例AIS患者平均年龄(13.9±0.51)岁。TceMEP、SSEP、自由运动肌电图(free-run EMG)和标准化的麻醉方案用于术中神经功能监测的研究。结果 EOS患儿采用后路半椎体或者全脊椎切除、短节段固定融合的手术方式。其中95.0%(19/20)的患儿获得术中稳定可靠的SSEP和TceMEP监测基线。1例(2岁,9 kg)直至手术截骨时未获得有效的TceMEP基线。本研究EOS患儿平均TceMEP基线波幅为(88.7±21.9)μV(范围:27~278μV),显著低于AIS患者的(346.7±24.2)μV,差异有统计学意义(t=2.162,P<0.01)。AIS患者均采用后路内固定,脊柱侧弯矫形植骨融合的手术方式。结论 TceMEP,SSEP和free-run EMG监测方法针对EOS患儿是准确可行的。监测结果可以为手术团队提供准确的神经功能信息,防止术后神经系统并发症的发生。     

单甘酯的高压液相色谱分析

采用ＨＰＬＣ测定了由纯油酸和棕榈酸合成产物中的单甘酯含量。油酸单甘酯的ＲＦ值较棕榈酸单甘酯的略高。ＨＰＬＣ法的ＭＧ测定值与柱色谱法相接近。     

用HPLC柱切换法血浆直接进样测定法莫替丁

本文采用HPLC柱切换技术,建立了以YWG-C18(3cm)为预处理柱,乙酸(0.2mol/L)为预处理流动相,血浆直接进样。在线(on-line)固相净化血浆样品;以Shimpack CLC-ODS(15 cm)为分析柱,甲醇-0.2 mol/L乙酸铵缓冲液(14:86)为流动相,267 nm波长检测,采用外标法定量。测定H₂-受体拮抗剂法莫替丁人体血药浓度的方法。该法净化过程简便,为自动净化血浆样品提供了一选择方法。回收率好(81.10～84.75%);法莫替丁的检测限约为2.4 ng,血浆中最低检出浓度约为12 ng/ml。日内变异系数为4.1%,日间变异系数为4.9%;在浓度为15～210 ng/ml血浆范围内呈线性关系。     

Spatial organization of the peripheral input to area 1 cell columns. I. the detection of ‘segregates’

Extracellular single neuron recording methods are used to study the RFs of neurons comprising area 1 cell columns in unanesthetized Macaca fascicularis monkeys. The RF data obtained in approximately radial microelectrode penetrations demonstrate that the RFs of neurons located within the same area 1 cell columns can differ strikingly, and that it is common for neighboring neurons to possess RFs differing greatly in size or configuration. However, the RF variations detected within a typical area 1 cell minicolumn (single cell radial column) appear to be substantially less than the variations observed for nearby neurons lying in different minicolumns.The RF data obtained from arrays of penetrations suggest that the skin representation in the forelimb region of area 1 is organized in a discontinuous, steplike fashion: as a mosaic of discrete 600 μm wide radial cell columns— segregates. Although the RFs of neurons of a segregate can vary substantially in size and configuration, they all share in common a single small area on the skin. The boundaries of a segregate can be mapped precisely because, unlike the situation for neurons located within the same segregate, some of the neurons located on opposite sides of a segregate boundary (belonging to different segregates) have non-overlapping RFs. Furthermore, it appears that within any given segregate there is no systematic shift in RF location as the electrode advances through a sequence of minicolumns. Systematic RF shifts occurred only when the electrode traversed the boundary between neighboring segregates.     

白喉乌头中生物碱的研究

目的 研究白喉乌头Aconitum leucostomum Worosch.中的生物碱.方法 采用柱色谱法分离纯化得到单体化合物,通过多种波谱方法鉴定其结构.结果 分离得到了10个生物碱,分别为:高乌甲素(I)、dehydroacosanine(Ⅱ)、冉乌碱(Ⅲ)、宋果灵(Ⅳ)、乌头碱(Ⅴ)、新乌头碱(Ⅵ)、去氧刺乌头碱(Ⅶ)、spicatine A(Ⅷ)、印乌碱(Ⅸ)、puberuline C(Ⅹ).结论 化合物Ⅴ～Ⅹ为首次从该植物中分离得到.     

Genetic effects induced by neutrons in Drosophila melanogaster I. Determination of absorbed dose.

A method to obtain the absorbed dose in Drosophila melanogaster irradiated in the thermal column facility of the Triga Mark III Reactor has been developed. The method is based on the measurements of neutron activation of gold foils produced by neutron capture to obtain the neutron fluxes. These fluxes, combined with the calculations of kinetic energy released per unit mass, enables one to obtain the absorbed doses in Drosophila melanogaster.     

Prognostic Significance and Prevalence of IgG Subtypes in Rh Haemolytic Disease of Fetus and Newborn

Many authors have reported poor prognostic value of anti-D antibody titer in the setting of Hemolytic Disease of Fetus and Newborn (HDFN). According to literature, HDFN cases with IgG1 and IgG3 have more severity compared to IgG2 and IgG4.Therefore, we planned this study to evaluate the prevalence and prognostic value of IgG subtypes in the setting of Rh HDFN. This was a retrospective study performed at a tertiary care center in north India from October 2015 to November 2017. Women with anti-D antibody were included in the study and categorized on the basis of presence of specific IgG subtype. “DAT IgG1/IgG3 ID” card (BIO-RAD) was used for determining the subclass of IgG. Various clinical, laboratory & interventional parameters were used to categorize fetal outcome in severe and non-severe cases. Perinatal outcome was then compared between women with different IgG subclass profile. Subclass distribution among 80 alloimmunized women was 26.2% for IgG1, 15% for IgG3, 46.2% for IgG1 + IgG3 and the rest had neither IgG1 nor IgG3. Severity of HDFN was significantly higher when IgG1 &/or IgG3 were present alone or in combination, compared to cases with absence of IgG1 or IgG3 (p value < 0.05). Risk of severe HDFN was significantly higher in the presence of IgG1 &/or IgG3 and the severity was highest when both IgG1 and IgG3 were present. We recommend that IgG subclass determination should be included in a multi—parameter protocol for more accurate prediction HDFN severity to ensure timely referral and intervention.     

HPLC法测定7-ACT相关杂质含量

目的：建立头孢曲松钠生产中中间体7-ACT相关杂质的HPLC测定方法.方法：采用十八烷基硅烷键合硅胶色谱柱（0.15m×4.6mm×1.0μm）,紫外检测器,检测波长：270nm,流动相：3.2g四庚基溴化铵置400mL乙腈中,加入44mL pH7.0磷酸盐缓冲液和4mL pH5.0枸橼酸盐缓冲液,加水稀释至1 000mL,过滤,流速：1.0mL/min,进样量20μL,自身对照法测定7-ACT的相关杂质含量.结果：7-ACT在0.06～3.6ppm浓度范围内呈现良好的线性关系（相关系数为0.9999）,精密度RSD（n=6）为1.4％;定量限：7-ACT、7-ACA达到0.048ppm,三嗪环达到0.012ppm.结论：本方法专属性强,操作简便,结果可靠,可用于7-ACT中相关杂质的测定.     

从何首乌提取物中制备二苯乙烯苷纯品的研究

目的 用层析柱分离、逆流色谱分离过程集成方法制备二苯乙烯苷纯品.方法 从二苯乙烯苷含量小于4％的何首乌提取物中,通过层析柱分离制得20％左右纯度的样品,再通过逆流色谱分离制备二苯乙烯苷纯品.结果 制得99％以上纯度的二苯乙烯苷样品.结论 方法可靠,在避光的情况下纯品稳定.     

Column selection for pharmaceutical analyses based on a column classification using four test parameters

This paper focuses on the usability of a previously developed column classification system, applied to pharmaceutical analyses. The separation of two drugs from their respective related substances was investigated on 65 new reversed-phase liquid chromatographic columns. The chromatographic procedure for fluoxetine hydrochloride was performed according to the method prescribed in the European Pharmacopoeia monograph while the separation of gemcitabine hydrochloride was carried out according to the United States Pharmacopeia monograph. It was shown that the column ranking system is a helpful tool in the selection of a suitable column.