清灵定眩汤治疗椎-基动脉供血不足眩晕临床分析

目的　探讨清灵定眩汤(QLDXD)治疗椎 基动脉供血不足眩晕(VBIV)的临床疗效。方法　对12 0例VBIV住院患者进行回顾性分析,其中91例为清灵定眩汤治疗组,2 9例为对照组(采用常规治疗) ,比较两组临床疗效及经颅多普勒(TCD)指标。结果　清灵定眩汤组临床疗效优于对照组(P <0 . 0 1) ,且能明显改善头痛、颈项不适、胸脘痞闷、恶心、痰多等伴随症状(P <0 .0 5 ) ,TCD指标较对照组也有明显改善(P <0 . 0 5 )。结论　清灵定眩汤对VBIV有确切的疗效,值得进一步研究和推广使用。     

葛根芩连汤不同配伍对黄芩苷、小檗碱含量的影响

目的研究葛根芩连汤在不同配伍的情况下黄芩苷、小檗碱含量的变化。方法采用反相高效液相法,选用PhenomenexC18柱(4.6mm×250mm),流动相为甲醇磷酸盐缓冲液(pH3),检测波长分别为280nm(黄芩苷)、346nm(小檗碱)。结果不同配伍对葛根芩连汤中黄芩苷、小檗碱含量影响显著。结论黄芩苷与小檗碱在煎煮后形成沉淀,从而使葛根芩连汤中的黄芩苷和小檗碱的含量大大降低。     

桃仁-红花"药对"不同配比的血府逐瘀汤对急性心肌缺血的保护作用

目的观察桃仁-红花“药对”不同配比的血府逐瘀汤抗垂体后叶素(Pit)致大鼠急性心肌缺血的作用。方法采用大鼠舌下静脉注射Pit造成急性心肌缺血模型,观察各给药组注射Pit后不同时间点Ⅱ导联心电图ST段变化以及血清乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。结果桃仁、红花不同配比的血府逐瘀汤对Pit所致缺血性ST段有明显改善作用,能明显增加血清SOD的活性,降低MDA、CK和LDH的含量,其中桃仁、红花配比为4∶3时效果最为显著。结论桃仁、红花不同配比的血府逐瘀汤均具有明显对抗Pit致大鼠急性心肌缺血的作用,其功效与桃仁-红花的不同配比之间关系不明显。     

八味地黄汤各药味对附子生物碱影响的电喷雾质谱研究

目的以八味地黄汤为研究对象,考察各药味对附子生物碱的影响规律,揭示附子配伍组方的科学内涵,为科学阐释八味地黄汤配伍规律提供化学依据。方法利用电喷雾质谱技术分别对八味地黄汤,附子加山茱萸、山药、肉桂、丹皮、泽泻、地黄、茯苓共煎液以及次乌头碱加山茱萸、山药共煎液进行系统考察。结果次乌头碱加山药、山茱萸,附子加山药等共煎液中次乌头碱含量较高;八味地黄汤、附子加山茱萸共煎液中次乌头碱含量较低;肉桂、丹皮、泽泻、地黄、茯苓等对附子生物碱的影响不明显。结论八味地黄汤中乌头碱和中乌头碱水解和溶出比较完全,次乌头碱的含量较少,次乌头碱的减少是由于水解以及溶出受到抑制导致。其中山茱萸对制附子起减毒作用,其减毒机制主要是抑制了制附子中次乌头碱的溶出。而山药则能导致附子毒性增加。     

补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究

目的:运用中药血清药理学方法研究补肾复方含药血清(BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用.方法:以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用,采用流式细胞术观察BS对谷氨酸所诱导PC12细胞凋亡的影响.结果:BS含药血清可以增强PC12细胞活力,拮抗谷氨酸的兴奋性神经毒作用且在20%浓度时作用最强,并且能够抑制谷氨酸诱导的PC12细胞早期凋亡.结论:补肾方BS可能具有神经保护作用.     

平肝熄风汤对脑出血大鼠海马细胞色素C氧化酶活性和细胞凋亡影响同步研究

目的 从神经元线粒体能量代谢和细胞凋亡的角度探讨中药复方的平肝熄风汤对脑出血神经元损伤的保护作用机制。方法 采用Ⅶ型胶原酶诱导大鼠脑出血模型，以组织化学方法结合灰度值半定量测定细胞色素C氧化酶(cytochromc c oxidase，CCO)活性，采用Tunel法检测细胞凋亡结果造模12h，脑出血大鼠海马CA1区CCO活性较假手术组明显降低(P＜0.01)，凋亡细胞较假手术组明显增多(P＜0．01)随后均呈进行性加重。用平肝熄风汤治疗后町维持其CCO活性，减少细胞凋亡。结论 脑出血神经元损伤与神经元CCO活性降低及细胞凋亡有关平肝熄风汤能维持线粒体关键酶CCO活性，改善细胞有氧代谢，减少细胞凋亡，此可能为本方对脑出血继发性神经元损伤保护机制之一。     

助黄汤对小鼠胚泡着床数的影响

目的:探讨助黄汤对小鼠胚泡着床数的干预作用。方法:选用8～10周龄昆明种雌性小鼠,分别给予不同剂量的助黄汤,以及克罗米芬、助黄汤加克罗米芬,灌胃4d,于妊娠第8d处死小鼠,观察平均着床胚泡数,并进行了毒性实验。结果:助黄汤组平均着床胚泡数明显高于克罗米芬组及正常组,且药理毒性实验为阴性。结论:助黄汤能提高小鼠胚泡着床数,可间接反应该药有可能促进卵泡发育,增加排卵数量及卵的受精数目,增强小鼠的生殖功能的作用;且无毒副作用。     

Intervention Effects of Jianpi Liqi Huoxue Decoction (健脾理气活血汤) on Lipid Peroxidative Liver Injury by Alcohol

Objective: To study the intervention effects of Jianpi Liqi Huoxue Decoction ( 健脾理气活血汤, JLHD) on lipid peroxidative liver injury induced by alcohol. Methods: The rat alcoholic model of liver disease (ALD) induced by Lieber-DeCarli liquid diet was established. Thirty-two male SD rats were randomly divided into 4 groups: the normal group ( n = 5), the control group ( n = 9), the model group ( n = 9) and the JLHD group (n = 9). From the 4th week after modeling, the rats were given JLHD or distilled water by gastrogavage respectively, and the samples of blood and liver tissues were taken out from the rats for determination by the end of the 8th week. The hepatic pathological changes were observed with HE staining; the liver injury related indices, including activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, γ-glutamyl transpeptidase (γ-GT) activity and triglyceride (TG) content in liver tissues, as well as the lipid peroxidation related indices, including malonaldehyde (MDA) content and nitric oxide synthase (NOS) activity in liver tissue, serum Fe2 level, and the anti-peroxidation capacity related indices, including superoxide dismutase (SOD) activity, glutathion ( GSH) content and reactive oxygen species (antiROS) activity in liver tissues were determined. Results: ( 1 ) There were obvious figures of fatty degeneration and inflammatory infiltration in liver tissues of the model group. As compared with the control group, in the model group, the activity of ALT and AST, and Fe2 content in serum, γ-GT and NOS activity, TG and MDA content in liver tissues were significantly higher ( P＜0.01 ), while the activity of SOD, GSH and anti-ROS in liver tissues were significantly lower ( P＜0.01 ). (2) The fatty degeneration and inflammatory infiltration of liver tissues in the JLHD group were significantly lessen as compared with those in the model group; and the abnormalities of all the indexes revealed in the model rats were restored to certain extent in the JLHD group,and especially significant were the levels of ALT activity, MDA content and Fe2 , which were nearly normal.Conclusion: JLHD has significant effects against alcoholic liver injury, the acting mechanism of which is likely to be related with its anti-lipid peroxidative effect.     

活血通脉汤防治全膝关节置换术后深静脉血栓33例

目的探讨自拟中药活血通脉汤预防全膝关节置换术(TKA)后深静脉栓塞的效果。方法33例住院患者,均予以全膝关节置换术后第一天给予活血通脉汤口服每日1剂,同时进行CPM机功能锻炼和配合足底和下肢静脉泵,手术后7天做下肢彩色多普勒检查,观察血栓形成与否。结果8侧下肢彩色多普勒检查显示深静脉形成。结果33例患中6例8侧出现DVT占18·6%;比较无任何预防措施的TKA术后DVT发生率有明显降低。所有病例均无肺栓塞临床表现。结论利用中药活血通脉汤防治人工膝关节术后深静脉血栓形成有效。     

当归六黄汤加减对32例混合痔术后盗汗患者临床疗效观察

[目的]探讨当归六黄汤加减对混合痔术后盗汗的疗效。[方法]对32例混合痔术后盗汗患者采用当归六黄汤加减。[结果]治愈11例(占34·4%),显效16例(占50·0%),有效5例(占16·6%)。[结论]当归六黄汤能有效治疗混合痔术后盗汗。