五味子藤茎化学成分分析及化学模式识别

目的:对蛟河地区不同采收期的五味子藤茎化学成分进行分析,为综合开发五味子藤茎资源提供参考依据。方法:以五味子甲素,单糖和寡糖,多糖的含量为统计指标,运用聚类分析统计学方法对其进行比较。结果:综合比较,11月和7月采集的藤茎与果实三种指标含量最接近,而就单一成分来讲,7月采集的藤茎五味子甲素含量最高,11月的多糖含量最高,9月的单糖,寡糖最高。结论:本实验首次运用化学模式手段对五味子藤茎的成分含量加以分析,为确定其最佳采收期提供参考依据。     

健骨蠲痛胶囊质量标准的研究

目的:制定健骨蠲痛胶囊的简便快捷的质量标准。方法:采用薄层色谱法(TLC),在同一块薄层板上鉴别健骨蠲痛胶囊中的白芍和土茯苓,在另一块薄层板上鉴别青风藤。用高效液相色谱法(HPLC)测定制剂中芍药苷的含量。结果:通过方法学考察,芍药苷的进样量在0.01-5.0μg范围内,呈良好的线性关系。芍药苷的平均回收率为98.6%(n=9),RSD=1.0%。结论:所用TLC和HPLC方法简便、准确、重现性好、精密度高。可用于健骨蠲痛胶囊质量控制。     

川木通的研究进展

对川木通近年来本草考证、资源现状、化学成分、品质评价及药理作用等方面的研究进展作一综述,为其进一步研究与应用提供了较全面的介绍。     

鸡血藤升白汤对放疗引起的小鼠骨髓抑制的影响

目的:研究鸡血藤升白汤对放疗引起的小鼠骨髓抑制的治疗作用及其机制。方法:成年雌性Balb/c小鼠32只,全身均匀照射DT550cGy引起小鼠放疗所致的骨髓抑制模型,造模后4小时内开始服用鸡血藤升白方汤药(简称鸡血藤升白汤),随机分作4组:对照组:8只,灌胃纯净水250mg/kg体重,低剂量组:8只,灌胃鸡血藤升白汤150mg/kg体重,中剂量组:8只,灌胃鸡血藤升白汤250mg/kg体重,高剂量组:8只,灌胃鸡血藤升白汤350mg/kg体重,每日2次,早晚各1次,中间间隔10小时,连续用药7天。照射前1天、及给药后第7天、13天、19天、25天、31天分别检测小鼠外周血白细胞数量。结果:骨髓抑制后小鼠白细胞明显下降,照射后1周最为明显;服药后2～3周白细胞明显恢复,中剂量组较其他各组有显著性差异,第四周基本恢复正常,鸡血藤升白汤明显促进外周血白细胞数量恢复。结论:鸡血藤升白方对放疗引起的小鼠骨髓抑制有明显的治疗作用。     

首乌藤超微饮片的高效液相色谱指纹图谱研究

目的 研究首乌藤超微饮片的指纹图谱.方法 色谱柱为大连依利特公司Hypersil BDS C18(4.6 mm×200 mm,5μm).流动相为乙腈-1%冰醋酸,柱温为35℃,流速为1.0 ml/min,检测波长为254 nm,进样量为20μl.结果 初步建立了首鸟藤超微饮片的HPLC指纹图谱,标定了9个共有指纹峰.结论 利用首乌藤超微饮片高效液相色谱(HPLC)指纹图谱可以较好地反映首乌藤超微饮片的内在质量.     

HPLC法测定静态加压溶剂提取马兜铃属植物中马兜铃酸

目的 建立并优化从马兜铃药材中提取马兜铃酸Ⅰ和Ⅱ的静态加压溶剂提取方法,考察各因素对提取效率的影响,并测定马兜铃、关木通、青木香和广防己中两成分的量.方法 以马兜铃酸Ⅰ和Ⅱ的量为指标,采取单因素考察的方法,分别优化提取溶剂、药材粒径、提取温度、提取压力、提取时间、冲洗体积、循环数目、药材加入量等影响因素.结果 优化后的条件为:甲醇提取,药材粉末粒径100～120目,提取温度120℃,提取压力10.3 MPa,静态提取时间10 min,冲洗体积40%,1个循环,药材加入量1.00 g.结论 本法可一次将马兜铃酸Ⅰ和Ⅱ从马兜铃等4种药材中完全提取,显示了良好的适用性.经比较,该法比传统的超声和索氏提取方法提取效率更高,节约溶剂,省时省力;静态加压溶剂法更适合于青木香中马兜铃酸的提取.     

Effects of "Caulis Sargentodoxae Formula" on Experimental Endometriosis in Rat

Objective To study the drug effects of"Caulis Sargentodoxae Formula" which clears away heat and blood clot on experimental endometriosis in rat, and to compare this effect with Danazol's effects.Method The model of endometriosis rat was induced by transplanting with endometrium surgically. The rats were divided into 6 groups randomly: the non-treatment group, the castrate group, Danazol group(80 mg/kg), "Caulis Sargentodoxae Formula" group (110 g/kg), the medium dose group(77.5 g/kg) and the low dose group(55 g/kg). After 3 weeks' treatment, the volume ofendometrium was detected by cutting the rats' belly open, drawing the materials from endometrium for tissue section, and doing the quantity analysis of endometrium by semiautomatic image analysis machine.Results There was no significant difference of the endometrium volumes between the low dose group and the non-treatment group(P＞0.05), other groups' endometrium volumes were significantly lower than those of the non-treatment group(P＜0.05). And apart from the low dose group, all other groups' heights of the endometrium epithelia were significantly lower than that of the non-treatment group(p＜0.01).Conclusion "Caulis Sargentodoxae Formula" can refrain the growth of the endometrium, and the effects are better than that of Danazol's.     

RP-HPLC测定首乌藤中大黄素的含量

目的建立首乌藤中大黄素的含量测定方法。方法采用RP-HPLC法,AlltimaODS柱(250mm×4.6mm,5μm),甲醇-0.1%磷酸溶液(85∶15)为流动相,流速1.0ml·min-1,检测波长256nm。结果线性范围为4.06~50.70μg·ml-1(r=0.9999),平均加样回收率为98.57%,RSD=2.52%(n=9)。结论所用方法准确、重复性好,可作为首乌藤的质量控制方法。     

Kamiuntanto increases prefrontal extracellular serotonin levels and ameliorates depression-like behaviors in mice

Kamiuntanto (KUT; Jia Wei Wen Dan Tang in Pinyin) is a traditional Japanese Kampo medicine that is used to treat psychological dysfunction. However, the mechanisms of action of KUT are not understood. To investigate the mechanisms underlying the ameliorative properties of KUT, the effects of KUT on abnormal behaviors of isolation-reared mice and on the prefrontal monoaminergic system were examined. KUT (1000 mg/kg) reversed encounter-induced hyperactivity and increased immobility in the forced swim test in isolation-reared mice, as also found for an antidepressant, fluoxetine (30 mg/kg). In vivo microdialysis showed that KUT (1000 mg/kg) transiently increased the level of extracellular serotonin (5-HT) in the prefrontal cortex. In contrast, an incomplete KUT formula excluding Bambusae Caulis (BC), a component herb of KUT, did not reverse abnormal behaviors of isolation-reared mice or increase prefrontal extracellular 5-HT. Furthermore, the antidepressant-like effect of KUT in the forced swim test was prevented by pretreatment with GR127935, a 5-HT_1B antagonist. These findings suggest that KUT may ameliorate depressive symptoms via 5-HTergic systems, and that BC plays an important role in the antidepressant-like effects of KUT.     

活血止痛制剂中三七的专属性鉴别方法研究

目的：建立活血止痛散和活血止痛胶囊中三七的专属性鉴别方法,并采用高分离度快速液相色谱-三重串联四极杆质谱（RRLC-QQQ/MS）对结果加以验证。方法：采用高效薄层色谱法研究三七与其混伪品人参、红参、三七茎叶的特征条带。以高效硅胶G薄层板为固定相,二氯甲烷-无水乙醇-水（70：45：6.5）展开,10%硫酸乙醇溶液显色,105℃加热至条带清晰。分别置紫外光灯（365 nm）和日光下检视。RRLC-QQQ/MS分析采用Agilent Rapid Resolution HT SB-C18（2.1 mm×100 mm,1.8 μm）色谱柱,以5 mmol·L^-1醋酸铵溶液-乙腈为流动相梯度洗脱。离子化模式为ESI-,碎裂电压100 V。结果：三七可检出三七皂苷R1,未检出人参皂苷Rb₃、人参皂苷Rf。人参和红参可检出人参皂苷Rb₃和人参皂苷Rf,未检出三七皂苷R1。三七茎叶可检出人参皂苷Rb₃,未检出三七皂苷R1或人参皂苷Rg₁。以三七对照药材、三七皂苷R1（应检出）和人参皂苷Rb₃（不得检出）为对照,可实现活血止痛制剂中三七的专属性鉴别和人参（红参）或三七茎叶的非法掺杂投料检查。6个厂家的93批样品均未发现人参、红参或三七茎叶冒充三七非法投料。RRLC-QQQ/MS与高效薄层分析结果一致。结论：该方法简便、快速、灵敏、准确,可为含三七中成药的质量控制提供参考。