Different doses of partial liver irradiation promotes hepatic regeneration in rat

The aim of this study is to investigate whether partial liver irradiation promotes hepatic regeneration in rat. Left-half liver of rat was irradiated to 10 Gy, and the Right-half to 0, 5, 10 and 15 Gy, respectively. Then, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were evaluated on 0 day, 15-day, 30-day, 45-day and 60-day after liver irradiation. Next, the serum HGF, NF-κB and TGF-β1 levels were also analyzed on 60-day after liver irradiation. Lastly, the cyclinD1 protein expression was appraised by western blots on 60-day after liver irradiation. ALT, AST and ALP levels were reduced compared with that of controls. The serum HGF, NF-κB and TGF-β1 levels, and the cyclinD1 protein expression in liver irradiation group were increased compared with that of controls group. However, hepatic regeneration of higher dose-irradiated cirrhotic liver was triggered a more enhanced regeneration, compared with that of higher doses group. In summary, these results suggest that different doses of partial liver irradiation promotes hepatic regeneration in rat.     

人脱细胞软骨细胞外基质对异种软骨种子细胞的影响

目的观察人脱细胞软骨细胞外基质(hACAM)对异种兔软骨种子细胞增殖和表型的影响。方法将差速梯度离心法制作的人脱细胞软骨细胞外基质,配制成0.5%的浆料,分别铺于6孔细胞培养板和96孔细胞培养板,形成1 mm厚的薄膜,以此培养板为实验组。空白对照组培养板仅使用单纯培养液培养。在培养板上培养兔关节软骨细胞。通过hochest33258染色验证人软骨细胞外基质脱细胞完全与否;分别在第5、15天两个时间点,通过倒置显微镜、甲苯胺蓝染色观察两种培养方法的兔软骨细胞的生长形态、细胞表型和增殖情况,用CCK-8细胞增殖实验比较两种培养方法在第1、3、7、10天的增殖情况。结果通过hochest33258染色发现差速梯度离心的人软骨细胞外基质脱细胞完全。通过倒置显微镜观察第5和15天的实验组细胞增殖情况优于空白对照组,甲苯胺蓝染色的结果也证明这个观点;CCK-8细胞增殖试验显示第7天hACAM组在细胞增殖方面明显地优于单纯培养液的对照组(P=0.0298),hACAM组的软骨细胞与空白组的软骨细胞在第10天的增殖情况相比没有统计学差异。结论 hACAM免疫原性低,无细胞毒性,能够很好地促进异种软骨细胞的增殖。     

经血源性子宫内膜干细胞及其修复外周神经损伤的应用前景

外周神经损伤带给患者长期病痛的同时严重影响了患者的生活质量,目前临床上除显微外科手术外,移植施万细胞(SC)也成为有效的治疗方法。然而SC移植的先天不足限制了其在临床上的大规模使用,因此,干细胞治疗神经损伤逐渐成为近年来的研究热点。而近来发现的经血源性子宫内膜干细胞(Men ESCs)凭借其丰富的来源,无创伤的分离方式以及较高的增殖活性和分化潜能等方面的优势获得广泛关注,并在成骨、心肌、肝脏、子宫内膜及中风等疾病的治疗过程中显示了良好的效果。因此,我们在明确外周神经损伤发生机制的基础上,着重阐明Men ESCs的生物学特性及其在治疗外周神经损伤的机制,以期为Men ESCs在外周神经损伤的治疗提供借鉴。     

羊富血小板胶细胞基质在2种牙周缺损模型中的修复效果

目的 探讨羊富血小板胶-牙周膜成纤维细胞基质(PRgel-PDLFs)促进不同原因造成的牙周缺损再生的效果.方法 用新鲜全血制取富血小板血浆(PRP),并加入催化剂与成纤维细胞一起培养合成PRgel-PDLFs基质;然后将24只山羊随机分为急性牙周缺损组和慢性牙周炎缺损组,模型建成后所有动物一侧植入PRgel-PDLFs基质于牙周缺损区,另一侧为手术空白组,术后10周,进行CT影像学检查和组织学测量分析.结果 10周后CT影像学和组织学检查显示:两组均在牙根面见新生牙骨质样组织、牙周膜样组织和牙槽骨样组织,且PRgel-PDLFs组再生的牙周组织明显多于空白组(P＜0.01);急性缺损组和慢性牙周炎缺损组的组织再生量相近,差异没有统计学意义(P＞0.05).结论 PRgel-PDLFs基质能够促牙周组织再生,且对急性牙周缺损和慢性牙周炎造成的牙周缺损修复效果相近.     

牙源性干细胞来源外泌体的功能研究新进展

近年来关于间充质干细胞来源外泌体的研究已成为热点,但有关牙源性干细胞来源外泌体的研究相对较少。相比于其他间充质干细胞,牙源性干细胞因易获取,并具有更强的免疫调节功能和组织再生能力而成为学者们研究的新方向。文章总结了近几年牙源性干细胞来源外泌体功能研究新进展,主要就牙源性干细胞来源外泌体的炎症调节功能、促组织再生功能和神经保护功能3个方面做一阐述。     

帐篷钉技术在牙槽骨修复与再生中的临床应用及操作规范

牙槽骨是牙医学的基础,牙槽骨缺损的修复与再生和口腔种植、口腔正畸、牙周及口腔修复等亚专业学科关系密切.因此,对牙槽骨进行有效的修复与重建具有重要的临床意义和社会效益.随着引导骨再生(guide bone regeneration,GBR)技术的发展,基于以"稳定为核心"的牙槽骨修复与再生理念,一种全新的骨增量技术快速发...     

The effect of SLActive surface in guided bone formation in osteoporotic-like conditions

Objectives: The aim of the study was to evaluate new bone formation under etched titanium (SLA) and modified‐etched hydrophilic titanium (modSLA) domes placed on the calvarium of healthy, osteoporotic and osteoporotic treated with bisphosphonates rabbits. Methods: Experimental osteoporosis was induced by ovariectomy (OV) and calcium‐deficient diet in 24 New Zealand female rabbits. Twelve OV rabbits were treated with weekly dozes of alendronate (Fosamax^®) (B) while 12 OV rabbits received no treatment (O). Another 12 rabbits were sham operated and used as healthy controls (C). At 6 weeks following OV, one modSLA and one SLA titanium dome were placed in the parietal bones of each rabbit. The animals were sacrificed at 30 and 120 days following the dome placement. Various histomorphometric measurements were performed in the most central of the undecalcified sections produced. Results: After 30 days of healing, in the C group, the total bone (TB) area was 37.6% and 37.0% under the modSLA and SLA domes, respectively. In the O group, the TB was 35.7% and 24.8%. In the B group, TB was 37.0% and 32.1%, respectively. After 120 days of healing, in the C group TB was 40.1% and 36.4%, respectively. In the O group, TB was 29.6% and 27.9%, respectively. In the B group, TB was 49.7% and 42.5%, respectively. Hierarchical analysis of variance showed that the type of titanium dome significantly influenced new bone and the amount of new bone being in contact with inner surface of the dome (BIC) independently of the observation period and group (P<0.05). The administration of bisphosphonates influenced the BIC (P<0.05). Conclusion: The use of modSLA surface may promote bone healing and osseointegration in osteoporotic rabbits, whereas administration of bisphosphonates may compromise the osseointegration of the newly formed bone at the early healing period. To cite this article:
Mardas N, Schwarz F, Petrie A, Hakimi A‐R, Donos N. The effect of SLActive surface in guided bone formation in osteoporotic‐like conditions.
Clin. Oral Impl. Res. 22 , 2011; 406–415.
doi: 10.1111/j.1600‐0501.2010.02094.x     

细胞膜片技术在牙周、牙髓牙本质再生中的应用研究

细胞膜片技术作为一种细胞移植的新方法,能够最大程度保留细胞外基质和细胞间黏附蛋白,减少细胞流失、避免外源性生物材料的应用且提高细胞植活率,被广泛关注。本文就近年来细胞膜片技术在牙周、牙髓牙本质再生中的应用研究作一综述。     

血管内皮细胞生长因子在颞下颌关节骨关节炎中的研究进展

颞下颌关节骨关节炎(temporomandibular joint osteoarthritis, TMJOA)是一种发病率高、危害大的常见颞下颌关节紊乱病,其主要病理过程包括软骨退变、滑膜炎和黏连形成。血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)是一种促进血管生长因子,近年来发现在TMJOA的关节盘、髁突软骨、滑膜组织及关节液中均有表达,故逐渐被认为是TMJOA形成的一种重要可疑因子。本文就VEGF在TMJOA发病过程中的作用及研究进展做一综述。     

载布洛芬的PLGA/PLA电纺纳米纤维GTR膜的实验研究

目的:通过静电纺丝技术制备载不同含量布洛芬(IPF)的聚乳酸-羟基乙酸共聚物(PLGA)/聚乳酸(PLA)纳米纤维引导组织再生(GTR)膜,评价其生物学性能。方法:使用静电纺丝技术制备PLA纳米纤维膜,扫描电镜观察其表面形貌,与EMT-6细胞复合培养,检测其生物相容性及机械阻挡功能;制备载不同含量布洛芬的PLGA/PLA纳米纤维膜,观察其表面形貌、MTT法检测人牙龈成纤维细胞(HGFs)在其上的增殖情况,同时检测其载药率及药物缓释情况。结果:PLA以及载入不同量IPF的电纺纤维的直径均为300 nm~5μm,载入布洛芬后,纳米纤维仍表面光滑、均一、连续;绝大多数的EMT-6细胞均黏附在PLA膜的表面,膜内部几乎没有细胞;HGFs与不同载药量的IPF-PLG/PLA电纺膜复合培养1、3、5、7 d时,各载药实验组在各时间点的OD值与空白对照组相比P>0.05;5%、10%、15%3种载药量的电纺膜的载药率均可达85%以上,在1周内的突释量分别为30%、60%、85%,突释较小,药物释放时间可持续10~28 d。结论:本实验所制备的载布洛芬PLGA/PLA电纺纳米纤维膜具有良好生物相容性、优异的阻挡功能及缓慢释药功能。