麻黄、五味子影响肺纤维化大鼠血管新生的差异性研究

目的 探讨麻黄、五味子对肺纤维化大鼠血管新生影响差异的作用机制.方法 选择SPF级Wistar雄性大鼠90只,随机设立假手术组、模型组、氢化可的松组、麻黄组、五味子组以及麻黄五味子组.采用博莱霉素A5(7 mg/kg)气管滴入方法建立肺纤维化模型,假手术组灌胃生理盐水(6 mL/kg)28 d;中药水煎液浓缩至每毫升含原药材1 g,麻黄组、五味子组分别灌胃麻黄(5g/kg)、五味子(2.5g/kg)水煎液28 d,麻黄五味子组灌胃麻黄水煎液(5g/kg)7 d后灌胃五味子水煎液(2.5g/kg)至28 d,氢化可地松组肌注氢化可地松20 mg/kg 28 d.于7、28 d放射免疫法检测各组大鼠内皮素-1(ET-1)、6-酮-前列腺素F1a(6-Keto-PGF1a)含量变化,免疫组化法观察肺组织微血管密度.结果 7 d时,模型组血浆ET-1含量升高,较假手术组差异有统计学意义(P＜O.05),6-Ke-to-PGF1a含量较假手术组有所升高,但差异无统计学意义;28 d时,麻黄五味子组与氢化可的松组血浆ET-1含量显著降低,与模型组比较差异有统计学意义(P＜0.05),各治疗组6-Keto-PGF1a含量较7 d时均有所升高.模型组肺微血管密度显著多于假手术组及各治疗组(P＜0.05),麻黄组对肺小动脉血管新生的抑制高于五味子组、麻黄五味子组.结论 麻黄对改善肺纤维化大鼠肺系数有积极作用,麻黄、五味子联合应用对肺纤维化的肺部小动脉损伤有抑制作用.     

白蒺藜有效组分对自发性高血压大鼠心肌纤维化及TGFβ1/CTGF表达的影响

目的 观察白蒺藜有效组分对自发性高血压大鼠(SHR)心肌纤维化以及相关细胞因子表达的影响,探讨白蒺藜有效组分改善心室重构、抑制高血压靶器官损伤的可能作用机制.方法 32只14周龄雄性自发性高血压大鼠随机分为模型组、卡托普利组、白蒺藜组、联用组,每组8只,同周龄雄性Wistar大鼠8只作为正常对照.卡托普利组采用卡托普利...     

鸦胆子水提组分大鼠长期毒性实验研究

目的 观察连续灌胃鸦胆子水提组分对大鼠的长期毒性,对其毒性定性、定位、定量以及与时间、剂量的相关性进行研究,明确毒性是其药效的延伸还是中药的自身毒性,以评价其长期用药的安全性.方法 80只Wistar大鼠随机分为鸦胆子水提组分高、中、低剂量组和正常对照组,大鼠连续灌胃鸦胆子水提组分27天,给药期间,每天观察动物的一般状况,每三天记录一次体重、日食量、日水量.停药后1天、27天分别检测血常规、血生化,测定主要脏器脏体比值并做病理组织检查.结果 鸦胆子水提组分灌胃27天可轻度影响大鼠的饮食和体重;对血常规无明显影响;血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平及肌酐(Cr)、尿素氮(BUN)含量均有所升高;心体比值、肾体比值也有所升高;高、中剂量组部分出现肾小球萎缩、肾小管玻璃样变等病理组织学变化.停药后27天除高剂量组血清Cr,BUN含量仍明显高于正常对照组外其他毒性改变均已恢复.结论 鸦胆子水提组分长期给药可对大鼠造成明显的肾毒性,且呈现明显的"时-毒"和"量-毒"关系,停药后毒性部分可逆.     

A traditional Chinese herbal preparation, Er-Zhi-Wan, prevent ovariectomy-induced osteoporosis in rats

Ethnopharmacological relevance

Er-Zhi-Wan (EZW), a classic Chinese formulation, which contains Fructus Ligustri Lucidi (FLL) and Herba Ecliptae (HE). EZW is widely used to prevent and treat various kidney diseases for its actions of nourishing the kidney and strengthening tendon and bone. Although recent reports indicate that EZW restrains osteoclastic bone resorption, its effects on the protection against define OVX-induced bone loss in mature rats have not been systematically investigated.

Materials and methods

Sixty 3-month-old female Sprague-Dawley rats were randomly assigned into sham-operated group (Sham) and five OVX subgroups, OVX with vehicle (OVX); OVX with Estradiol Valerate (EV, 0.4 mg/kg body weight/day); OVX with EZW of graded doses (9.0, 4.5, or 2.25 g/kg/day). Daily oral administration of EV and EZW on 5th week for 26 weeks. Bone turnover markers (Serum alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), osteocalcin (OCN), deoxypyridinoline (DPD)), other parameters, including serum calcium (S-Ca), serum phosphorus (S-P), urine calcium (U-Ca), phosphorus (U-P), and bone mineral density (BMD) of the femur, 4th lumbar vertebra and tibia, bone biomechanical properties and trabecular microarchitecture parameters were measured.

Results

Administration of EZW could significantly prevent ovariectomy-induced bone loss, biomechanical reduction, deterioration of trabecular microarchitecture and the body weight gain without affecting the weight of the uterus, and increased S-Ca, S-P levels, decreased level of bone turnover markers and U-Ca, U-P levels in ovariectomized rats.

Conclusion

The present study indicated that EZW had a definite antiosteoporotic effect without hyperplastic effect on uterus, and it might be a potential alternative medicine for treatment of postmenopausal osteoporosis.     

Fructus panax ginseng extract promotes hair regeneration in C57BL/6 mice

Ethnopharmacological relevance

Radix panax ginseng (Panax ginseng C.A. Meyer, Araliaceae, RPG) has been documented to possess hair growth activity and widely used to treat alopecia, while no report has been issued to date on the effect of Fructus panax ginseng (FPG) on hair regeneration.

Materials and methods

To investigate the effects of FPG extract on the proliferation of human hair dermal papilla cells (DPCs) and on the promotion of hair regeneration in C57BL6 mice, cell proliferation was evaluated in cultured DPC by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and measured the expressions of Bcl-2 and Bax by immunoblot assay. We also compared the effects of topical FPG extract (1 and 10 mg/ml, 100 μl/d) with the effects of minoxidil as a positive control (5%, 100 μl/d) or vehicle control (30% ethanol) on the depilation-induced hair cycling in 7 week-old-C57BL/6 mice.

Results

FPG extract significantly increased the proliferation of DPCs in dose and time dependent manners (P < 0.05, P < 0.01 and P < 0.001). FPG extract also enhanced Bcl-2 expression and decreased Bax expression compared with control (P < 0.01). Moreover, significant elongations of anagen phase during hair cycle after application of FPG were evaluated by photographical and histological observations.

Conclusions

FPG extract improves the cell proliferation of human DPCs through anti apoptotic activation. Topical administration of FPG extract might have hair regeneration activity for the treatment of hair loss.     

复方灵通胶囊稳定性试验中吴茱萸碱和吴茱萸次碱的含量变化

目的 考察复方灵通胶囊稳定性试验中吴茱萸碱和吴茱萸次碱的含量变化.方法 建立复方灵通胶囊中吴茱萸碱和吴茱萸次碱的HPLC测定方法;分别采用加速稳定性试验和长期稳定性试验,测定加速试验条件下0、1、2、3、6个月及长期稳定性条件下0、3、6、9、12个月取样点时样品中吴茱萸碱和吴茱萸次碱的量;比较长期稳定性条件下12个月...     

牛蒡ITS 序列与药材质量的相关性研究

目的 研究牛蒡ITS序列与牛蒡子药材质量相关性.方法 采集全国26个不同产地的牛蒡子药材,采用PCR扩增后测定ITS序列,HPLC法对牛蒡子药材中牛蒡苷含量进行测定,应用ClustaiX( 1.81),Mage 4.0,SPSS 13.0等统计软件进行遗传多样性、基因分型、相关性等分析.结果 测得了26份牛蒡的ITS序列全长,在GenBank中注册,获得登记号,测定牛蒡子药材中牛蒡苷含量、千粒重,统计分析牛蒡的基因分型与药材质量具有相关性.结论 为揭示牛蒡子道地药材的分子形成机制奠定了基础.     

基于动物行为学考察萸黄连“反制”前后的药性差异研究

目的:研究萸黄连"反制"前后的寒热药性的差异性。方法:选用KM小鼠,依据动物行为趋向性,分别给予黄连、吴茱萸、萸黄连,考察不同药物对小鼠温度趋向性的影响,并结合测定小鼠肝脏ATP酶的活力,探讨不同药性药物对小鼠机体能力代谢的影响。结果:吴茱萸组小鼠体现出"趋寒性",能使小鼠ATP酶活性增强;黄连和萸黄连组小鼠体现出"趋热性",并抑制小鼠ATP酶活性;黄连组和萸黄连组相比,黄连组小鼠的"趋热性"较萸黄连组强(P<0.05),萸黄连组小鼠的钠钾ATPase活力较黄连组有显著提高(P<0.05);结论:吴茱萸体现出"热性",黄连和萸黄连均体现出"寒性",但萸黄连与黄连相比,"寒性"降低。此结论与萸黄连"以热制寒"的"反制"传统炮制理论相符。     

RP-HPLC法测定余甘子中诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯的含量

目的:建立RP-HPLC法同时测定余甘子(Phyllanthus emblica L.干燥成熟果实)中诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯含量。方法:采用Diamonsil C18柱(250 mm×4.6 mm,5μm),以甲醇-0.2%醋酸水溶液(19:81,v/v)为流动相;检测波长为275 nm;流速为1 mL.min-1;柱温为30℃。结果:诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯线性范围分别为0.235～1.176μg、0.185～0.924μg、0.418～2.09μg;平均回收率(n=6)分别为101.5%,99.20%,101.5%。结论:建立的方法简便,重现性好,可用于余甘子中诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯的含量测定。     

HPLC法测定山茱萸中科罗索酸的含量

目的:建立高效液相色谱法测定山茱萸中科罗索酸的含量。方法:采用Inertsil ODS-3(150 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-水-磷酸(82∶18∶0.2),流速1.0 mL.min-1,检测波长205 nm,柱温30℃。结果:科罗索酸进样浓度在59.86～598.6μg.mL-1范围内与峰面积呈良好的线性关系,平均回收率(n=6)为98.7%(RSD=1.0%)。结论:该方法分离度好,专属性强,重复性好,简便易行,可用于山茱萸中科罗索酸的含量测定。