Effects of AR-L 115 BS (Sulmazol), a new cardiotonic agent, in coronary artery disease: improved ventricular wall motion, increased pump function and abolition of pacing-induced ischemia

AR-L 115 BS (Sulmazol) is a new noncatechol, nonglycosidic cardiotonic agent. In 17 patients with significant coronary artery disease, the influence of AR-L 115 BS on hemodynamics and regional wall motion was investigated under the following conditions: 1) control, 2) the immediate postpacing period without medication, and 3) the postpacing period under the peak influence of AR-L 115 BS, 2 mg/kg intravenously. During the postpacing phase without medication, all patients developed ischemia (angina, ST segment alterations, increase of mean left ventricular end-diastolic pressure from 13 to 30 mm Hg), left ventricular pump function diminished and overall regional wall motion showed a tendency to decrease (p greater than 0.05). However, during the postpacing period with AR-L 115 BS medication, ischemia was abolished (no angina; mean left ventricular end-diastolic pressure decreased to 13 mm Hg; hemodynamic variables returned to control levels and left ventricular pump function showed some improvement while overall regional wall motion showed tendencies to improve. A comparison of alterations of hemodynamics and regional wall motion during the postpacing phase without medication with those under the influence of AR-L 115 BS shows that overall left ventricular pump function and regional wall motion improved while angina and an increase in left ventricular end-diastolic pressure were prevented. It is concluded that AR-L 115 BS improves left ventricular pump function and regional wall motion in coronary artery disease without inducing ischemia, probably by means of a reduction in extravascular resistance.     

老年与成年大鼠纹状体多巴胺含量及黑质部凋亡相关蛋白表达的研究

目的 研究老年与成年SD大鼠纹状体多巴胺含量及黑质部凋亡相关蛋白Bad、Bcl-2的表达情况,并探讨两者之间的关系.方法 取老年SD大鼠6只作为老年组,成年SD大鼠6只作为成年组;采用高效液相色谱-质谱联用法测定纹状体内多巴胺含量;免疫印迹法检测黑质Bad、Bcl-2的表达.结果 老年组纹状体DA含量(4.13±0.209 ng/mg)低于成年组(5.65±0.188ng/mg)(P＜0.05);老年组黑质部抗凋亡蛋白Bcl-2的表达(1.08±0.047)低于成年组(1.36±0.085)(P＜0.05);促凋亡蛋白Bad的表达(0.79±0.051)高于成年组(0.57±0.071)(P＜0.05).结论 老年SD大鼠纹状体DA含量降低,抗凋亡蛋白Bcl-2表达降低,促凋亡Bad表达增高.     

84例染色体异常核型及临床分析——附5例世界首报染色体异常核型

目的 探讨人类染色体异常与疾病的关系,并进行临床分析.方法 2010年1-12月,对498例有不良孕产史、不孕症、习惯性流产、生育畸形、死胎、死产、智力低下、表型异常、有遗传病家族史等前来就诊和咨询者进行细胞遗传学检查.取受检者肝素抗凝外周血,无菌条件下进行淋巴细胞培养,G显带,计数30个分裂相,分析核型3个,异常者加倍分析.必要时行C和R显带分析.结果 498例染色体检查中,染色体异常84例,染色体异常检出率为16.87％.其中常染色体异常79例,占94.05％;性染色体异常5例,占5.95％;经中国医学遗传中心鉴定,世界首报染色体异常核型5例,占染色体异常的5.95％.结论 染色体核型分析可有效检出染色体病携带者,降低人群发病率,对于明确病因、干预、再次妊娠进行生育指导、治疗及预后十分必要.     

团体认知干预联合个性化营养护理对前列腺癌手术患者的影响

目的:探讨团体认知干预联合个性化营养护理对前列腺癌手术患者的影响。方法:将118例前列腺癌手术患者随机分为常规组和联合组各59例,常规组行个性化营养护理,联合组行团体认知干预联合个性化营养护理,比较两组护理效果。结果:联合组患者干预后焦虑自评量表(SAS)、抑郁自评量表(SDS)、生活质量评分及免疫功能均优于常规组(P<0.05)。结论:团体认知干预联合个性化营养护理能有效改善前列腺癌手术患者的不良情绪和生活质量,提高患者术后免疫功能。     

医护一体化干预对食管癌根治术患者疾病不确定感、不良情绪的影响

目的:探讨医护一体化干预对食管癌根治术患者疾病不确定感、不良情绪的影响。方法:选取2017年10月至2019年10月聊城市第三人民医院肿瘤科收治的食管癌根治术患者126例为研究对象。采用随机数字表法分为观察组和对照组,各63例。对照组患者给予常规食管癌根治术护理干预,观察组患者在此基础上给予医护一体化护理干预,观察并比...     

Melatonin prevents hepatic injury-induced decrease in Akt downstream targets phosphorylations

Melatonin is a potent scavenger of reactive oxygen species and a strong antioxidant. Melatonin exerts protective effects against damage by the enhancing the Akt signal pathway, thus regulating apoptotic cell death. Akt phosphorylates pro-apoptotic proteins such as Bad and FoxO1 and inhibits the pro-apoptotic functions of these proteins. This study investigated the protective effects of melatonin through Akt and its downstream targets, Bad and FoxO1, in hepatic ischemia-reperfusion (I/R) damage. Adult mice were subjected to 1 h of hepatic ischemia and 3 h of reperfusion. Hepatic ischemia was induced by occlusions of the hepatic artery, portal vein, and bile duct. Melatonin (10 mg/kg, i.p.) or vehicle was administrated 15 min prior to ischemia and just before reperfusion. Serum aspartate aminotransferase and alanine aminotransferase levels were higher in I/R group than in sham-operated group. Melatonin attenuated increases in these levels. Moreover, melatonin attenuates injury-induced increases in positive TUNEL staining in hepatic tissues. Hepatic I/R injury induced reductions in the Akt up-stream target, PDK1 phosphorylation. The levels of phospho-Akt, phospho-Bad, and phospho-FoxO1 were decreased in vehicle-treated animals. However, melatonin prevented hepatic I/R injury-induced decreases in these proteins levels. Moreover, the interaction levels between phospho-Bad and 14-3-3 and between phospho-FoxO1 and 14-3-3 are reduced in vehicle-treated animals, and melatonin attenuated decreases in the binding levels of these proteins. 14-3-3 exerts an anti-apoptotic function by sequestration of Bad and FoxO1. These findings suggest that melatonin exerts protective effects in case of hepatic I/R damage by maintaining the binding of phospho-Bad and 14-3-3 and the binding of phospho-FoxO1 and 14-3-3, thus preventing activation of apoptotic cell death.     

Expression of the bcl-2 family of pro- and anti-apoptotic genes in multiple myeloma and normal plasma cells: regulation during interleukin-6(IL-6)-induced growth and survival

Aberrant expression of genes regulating apoptosis/survival seems to be essential in the stepwise development of human multiple myeloma (MM). In this paper we have compared the expression of bcl-2 family pro- and anti-apoptotic genes in MM cell lines, primary MM cells and normal plasma cells. The Bcl-2, Mcl-1, Bcl-xL/S, Bcl-w, Bax, Bak, and Bad were shown to be expressed in both malignant and non-neoplastic, normal plasma cells. Quantitative analysis revealed that the malignant phenotype seemed to correlate with an elevated expression of Mcl-1, a decreased expression of Bax and, to a lesser extent, an increased Bcl-2/Bax expression ratio. The possible influence of interleukin-6 (IL-6) in regulating the expression of the bcl-2-related genes was also examined. Using the IL-6-dependent MM cell lines U-1958 and U-266-1970 it was clearly shown that IL-6 deprivation induced cell cycle arrest in both cell lines, whereas apoptosis was only detected in the U-1958 cells. Furthermore, the anti-apoptotic proteins Bcl-2, Mcl-1 and Bcl-xL were down-regulated, while the expression of the pro-apoptotic Bax protein was increased. To conclude, we suggest that the expression pattern of the Bcl-2 family of proteins separates the malignant phenotype of MM from normal plasma cells, and that the protecting effect of IL-6 may be conducted via an altered balance between these proteins.     

护理干预对ICU清醒患者不良情绪的影响

目的:探讨护理干预对ICU清醒患者不良情绪的影响,并制定有利于患者健康的相应对策,为ICU临床护理提供依据.方法:将124例ICU清醒患者随机分为干预组61例和对照组63例,对照组给予常规护理,干预组在对照组基础上给予信息告知护理、社会支持护理、睡眠护理、舒适护理和非语言沟通等干预措施.应用焦虑自评量表和抑郁自评量表进行调查.结果:干预组与对照组比较,焦虑和抑郁程度差异有统计学意义(P<0.05).结论:护理干预可缓解ICU清醒患者不良情绪,护理人员应在实施护理的过程中灵活应用信息告知护理、社会支持护理、睡眠护理、舒适护理和非语言沟通等干预措施提高护理服务质量.     

缺氧后适应与心肌营养素-1对心肌细胞缺氧复氧的保护作用及机制探讨

目的 探讨缺血后适应与心肌营养素-1（CT-1）对心肌细胞缺氧复氧的保护作用及其机制.方法 将体外培养的H9C2细胞株分为正常对照组（a组）、缺氧复氧组（b组）、缺氧复氧＋缺氧后适应组（c组）、缺氧复氧＋缺氧后适应＋ CT-1组（d组）、缺氧复氧＋缺氧后适应＋CT-1＋ ERK抑制剂组（e组）、缺氧复氧＋缺氧后适应＋CT-1＋二甲基亚砜组（f组）,MTS法检测各组细胞存活率、流式细胞仪检测细胞凋亡率、Western blot检测细胞外调节激酶（ERK1/2）和磷酸化ERK1/2（p-ERK1/2）的表达、Q-PCR检测Bad mRNA的表达.结果 与a组比较,其他各组细胞存活率下降,细胞凋亡率、Bad mRNA和p-ERK1/2（除外e组）表达量增加,P均＜0.05;与b组比较,c组细胞存活率、p-ERK1/2表达量均增加,Bad mRNA表达、凋亡率下降,P均＜0.05;与c组比较,d组细胞存活率、p-ERK1/2表达量均增加,细胞Bad mRNA表达、凋亡率下降（P均＜0.05）;与d组比较,e组细胞存活率下降,细胞凋亡率、Bad mRNA表达量增加,p-ERK1/2表达量下降;d、e组各项指标指标比较差异无统计学意义.结论 缺氧后适应可减少心肌细胞缺氧复氧损伤,联合CT-1后保护作用明显加强,而ERK1/2抑制剂可阻断这种保护作用;该保护机制可能与CT-I激活ERK1/2信号通路,下调Bad mRNA的表达有关.