人与大鼠补体C5b—9复合物的提取及相应抗体的制备

目的 提取人及大鼠血清补体Ｃ５ｂ－ｐ复合物并制备抗体。方法 利用兔红细胞能直接活化人补体旁路途径的特性首先获得人或大鼠补体攻击破裂的兔红细胞膜,然后用脱氧胆酸钠溶解后的经超速离心提取Ｃ５ｂ－９复合物。结果 纯化的Ｃ５ｂ－９复合物电镜负染观察有其独特的形态。     

Application of a hybrid ion trap/time-of-flight mass spectrometer in metabolite characterization studies: Structural identification of the metabolism profile of antofloxacin in rats rapidly using MS information and accurate mass measurements

We describe herein, a very effective way in the rapid identification of metabolites for antofloxacin based on a hybrid ion trap (IT)/time-of-flight (TOF) mass spectrometer technique. The purified samples were separated by a reversed-phase C₁₈ column under a gradient elution, antofloxacin and its metabolites were detected by the on-line IT/TOF detector in scan mode. The identification of the metabolites and elucidation of their structure were performed by comparing the changes in molecular masses (ΔM), calculating compound-based component by Formula Predictor software, and defining sites of biotransformation based upon mass shifts of diagnostic fragment ions according to the accurate MSⁿ spectral information. In this case, we used such strategies for the identification of the metabolism for antofloxacin, and six metabolites of antofloxacin were found in rats for the first time.     

突发事件急救现场伤病员身份标识研究

以唯一标识突发事件伤病员为目的,概述身份标识研究现状,确定突发事件急救现场伤病员身份标识所需采集的信息以及具体流程.这对于伤病员的救治和康复以及提高应急医疗卫生救援服务水平具有重要意义.     

临床标体中钩端螺旋体及其L型的检测与鉴定

目的　为探讨钩体 Ｌ 型在钩体病患者体内是否存在及是否具有致病作用。方法　对患者血、 Ｃ Ｓ Ｆ 进行 Ｍ Ａ Ｔ；对患者血、 Ｃ Ｓ Ｆ 及尿液进行钩体及其 Ｌ 型培养； 对培养出的钩体 Ｌ 型进行免疫酶染色、免疫荧光染色、 Ｓ Ｄ Ｓ－ Ｐ Ａ Ｇ Ｅ、 Ｄ Ｉ Ｂ Ａ及电镜等方法鉴定。结果　 Ｍ Ａ Ｔ 阳性率为１９１７ ％ （６０３１３） ； 钩体普通培养阳性率１１８８ ％ （４１３４５） ； 钩体 Ｌ 型阳性率２４９３ ％ （８６３４５） 。培养出的 Ｌ 型经上述方法鉴定为钩体 Ｌ 型。结论　钩体 Ｌ 型培养应与 Ｍ Ａ Ｔ 及钩体普通培养同时进行，以防漏诊。     

南海海绵Clathria fasciculate化学成分的研究(Ⅱ)

从中国南海海绵Clathria fasciculate的乙酸乙酯相分离鉴定出4个有机化合物，经GC-MS，FAB，IR，^1HNMR,^13CNMR和COSY等波谱技术确定它们分别是：胆甾-4-烯-3-酮（Ⅰ）；豆甾-4-烯-3-酮（Ⅱ）；（Z）-17-二十四碳烯-1-醇（Ⅲ）和1-（17Z-二十四碳烯基）-甘油醚（Ⅳ），为首次有关该种属海绵化学成分研究系列的第二次报道。     

戊己丸的双波长薄层扫描鉴定

中成药戍己丸是由黄连、吴茱萸、白芍三味药制成的水泛丸。经乙醇提取,以硅胶 G 为固定相,正丁醇-醋酸-水为流动相,薄层分离之后,用双波长扫描法得到扫描图谱,然后用标准药材做阴阳性对照,从而鉴别出戊己丸中各单味药。方法简便、灵敏可靠、重现性好。     

近年来西洋参鉴别方法研究进展

对近年来西洋参的各种鉴别方法的研究概况进行了综述，为西洋参的真伪鉴别和深入研究提供了资料。     

ATB细菌鉴定仪与手工法鉴定结果比较分析

目的通过分析比较细菌鉴定仪与手工法鉴定结果，以期建立一套现实可行的仪器与手工相结合的鉴定方法。方法用ATB细菌鉴定仪与手工法分别鉴定29株来自质控及临床标本菌株。结果鉴定29株细菌其中有2株细菌仪器与手工法的鉴定结果不符。结论合理应用传统的手工法不失为仪器鉴定的一种辅助方法。手工法也可将细菌准确地鉴定到种，为没有细菌自动鉴定仪的基层医院增强了细菌鉴定的信心。     

Facial recognition and laser surface scan: a pilot study

Surface scanning of the face of a suspect is presented as a way to better match the facial features with those of a perpetrator from CCTV footage. We performed a simple pilot study where we obtained facial surface scans of volunteers and then in blind trials tried to match these scans with 2D photographs of the faces of the volunteers. Fifteen male volunteers were surface scanned using a Polhemus FastSCAN Cobra Handheld Laser Scanner. Three photographs were taken of each volunteer’s face in full frontal, profile and from above at an angle of 45° and also 45° laterally. Via special software (MIMICS^® and Photoshop^®) the surface scans were matched with the photographs in blind trials. The matches were graded as: a good fit; possible fit; and no fit. All the surface scans and photos were matched correctly, although one surface scan could be matched with two angled photographs, meaning that the discriminatory value was 86.7%. We also tested the surface scanner in terms of reliability in establishing point measures on skulls, and compared with physical measurements performed by calipers. The variation was on average 1 mm for five cranial measures. We suggest how surface scanning might be applied in forensic facial identification.