旱田草的生药学研究

目的 对旱田草进行生药学研究.方法 鉴别旱田草的性状,并按2010年版《中国药典》附录中的方法测定旱田草的水分、总灰分、酸不溶性灰分、浸出物含量等.结果 初步建立了生药学鉴别方法,其水分不得过13.0％,总灰分小于18.0％,酸不溶性灰分低于9.0％,醇溶性浸出物大于13.0％.结论 所用方法可为制定旱田草药材质量标准提供参考.     

洁治术后抛光处理在吸烟患者牙周基础治疗中的疗效评价

目的观察吸烟患者超声洁牙及喷砂后橡皮杯抛光对菌斑指数和出血指数的影响。方法选择40例要求沽牙的吸烟患者,进行超声洁治、喷砂抛光后,采用自身对照设计,随机把同一受试者左右侧牙齿分为实验组（洁治喷砂加橡皮杯抛光）和对照组（洁治喷砂）,观察菌斑指数和出血指数的变化。结果在治疗后3个月、6个月的菌斑指数检查显示,实验组均明显低于对照组（P〈0．05）,治疗后6个月,实验组的出血指数明显低于对照组（P〈0．05）。结论洁治喷砂后用橡皮杯抛光处理更能有效提高牙面的清洁度、光滑度,不利于牙菌斑、色素的再附着。     

Tyrosine Phosphorylation of the Proto-oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT-7

The vav proto-oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte-macrophage colonystimulating factor, interleukin-3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT-7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT-7. These data suggest that tryosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells.     

鼠疫坟茔尸骨检验结果分析

目的 检测死于50年前的鼠疫病人尸骨及尸骨周围土壤是否存在鼠疫菌。方法 利用鼠疫细菌学和血清学方法对尸骨和土壤进行检验。结果 尸骨及土壤中没有分离到鼠疫菌。结论 在尸体腐烂过程中其条件不利于鼠疫菌存活，加之50～60年风干的环境亦可导致鼠疫菌的死亡。     

鸡骨香药材水分、灰分及浸出物含量测定

目的：建立鸡骨香的水分、灰分及浸出物的限度检查,为鸡骨香药材的质量标准提供依据.方法：采用2010年版《中国药典》方法对鸡骨香中的水分、灰分及浸出物进行测定,并对浸出物的提取条件进行优化.结果：测得鸡骨香药材中水分不得超过11.4％,总灰分不得超过7.0％,浸出物测定用稀乙醇做溶剂,以热浸法进行测定,所得浸出物不得低于14.0％.结论：鸡骨香的浸出物、水分、灰分含量可作为鸡骨香药材质量标准的重要指标之一.     

麻黄饮片的质量标准研究

目的比较不同产地麻黄饮片质量,制定麻黄饮片质量的参考标准.方法采用 HPLC法测定各饮片的麻黄碱和伪麻黄碱含量,依照<中国药典> 2000年版一部的方法测定各饮片的浸出物、灰分、水分、杂质及灰屑等;并采用经验法进行加速稳定性试验.结果麻黄碱含量生品为 0.995 %～ 1.589 %,蜜炙品为 0.855 %～ 1.557 %;伪麻黄碱含量生品为 0.560 %～ 2.087 %,蜜炙品为 0.508 %～ 1.902 %;水溶性浸出物生品为 8.83 %～ 18.30 %,蜜炙品为 14.81 %～ 27.45 %;醇溶性浸出物生品为 7.74 %～ 18.83 %,蜜炙品为 14.15 %～ 27.34 %;总灰分生品为 6.49 %～ 10.29 %,蜜炙品为 6.34 %～ 10.24 %;酸不溶灰分生品为 0.19 %～ 0.42 %,蜜炙品为 0.18 %～ 0.42 %;平均含水量生品为 8.10 %( s=0.3961),蜜炙品为 4.02 %( s=0.4674);平均所含杂质及灰屑量生品为 2.02 %( s=0.1954),蜜炙品为 2.01 %( s=0.2209).结论 该研究为建立完整、科学的麻黄饮片质量标准提供了参考依据.     

某纯碱生产企业职业病危害因素现状调查与评价

目的 了解某纯碱生产企业存在的职业病危害因素和采取的职业病危害防护措施,为职业病防治工作提供依据。方法 采用现场职业卫生学调查和工作场所职业病危害因素检测方法,进行职业卫生现况调查分析。结果 粉尘、氨气、碳酸钠、一氧化碳、氯化氢、硫化氢、噪声、高温为该厂存在的主要职业病危害因素,其中粉尘超标率为21.2%、氨气超标率为14.9%、碳酸钠超标率为13.8%、噪声超标率为11.8%,氯化氢、硫化氢、高温均符合国家卫生标准要求。结论 该企业采取的职业病危害防护措施基本可行,职业卫生管理体系较为完整,职业卫生管理水平较高,但某些职业病危害因素存在超标点,应着重改善作业环境,提高防护措施,控制有害因素的浓度和强度,防止职业病的发生。     

Increased Ash Contents and Estimation of Dissolution from Chemical Changes due to In-Vitro Fluoride Treatments

The in-vitro fluoride treatment technique has been introduced to investigate the composite behavior of bone tissue. Bone tissue with different mechanical properties can be obtained by varying the concentration, pH and immersion time in fluoride ion solutions. The chemical and physical changes in intact pieces of bone treated in-vitro with different concentrations of fluoride ions are studied. The amount of bone mineral that does not contribute to the mechanical behavior of bone tissue is estimated from the dissolution occurring in the fluoride treated bones. Cortical bones from 18-month-old steers were treated in-vitro with 0.145, 0.5 and 2.0M sodium fluoride (NaF) solutions for three days. The dissolved bone mineral precipitates as calcium fluoride-like (CaF 2 /P with some phosphate [P] ions) and fluorapatite(FAp)/fluorhydroxyapatite-(FHAp)-like materials within the bone tissue. The dissolution estimated from the presence of the precipitated fluoride phases is 5.6, 11.7, and 13.1% of the initial bone mineral content for the 0.145M, 0.5M, and 2.0M NaF treatments respectively. Estimates of dissolution based on the measurements of phosphate and carbonate ions are lower and higher respectively when compared to the fluoride ion measurements. The wet and dry densities decreased slightly due to dissolution and re-precipitation while the ash content (ratio of the ash weight to dry weight) increased a small amount with increasing concentration of fluoride ion treatments. The increased ash content was due to the excess loss of water in the fluoride treated bones as compare to controls (untreated bone samples) during the drying process. The increased removal of water during the drying process may explain the increased ash contents in some in-vivo treatments.