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131.
目的探究青蒿治疗人急性髓系白血病(acute myeloid leukemia,AML)的作用机制。方法根据口服生物利用度(OB)和类药性(DL)从中药系统药理学技术平台(TCMSP)中筛选出青蒿的活性成分、作用靶点,在GeneCards数据库中寻找急性髓系白血病相关靶点,将青蒿作用靶点与AML相关靶点取交集,筛选出青蒿治疗AML的活性成分及作用靶点。借助Cytoscape 3.7.2软件,构建青蒿活性成分-作用靶点网络,选出关键化合物。通过String平台构建靶蛋白相互作用网络(PPI),选出关键靶点。通过DAVID在线分析平台,对靶点基因进行GO富集分析和KEGG代谢通路分析。结果从青蒿中筛选获得22个有效活性成分,104个作用靶点,与AML有关的作用靶点94个,关键靶点14个,通路涉及免疫调节、细胞凋亡、氧化应激等,这些作用通路可能是青蒿防治AML的活性途径。结论初步研究了青蒿治疗AML的作用机制,青蒿具有多成分、多靶点的特点,可以通过多个靶点、多条通路来发挥治疗AML的作用。 相似文献
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133.
人RANTES基因在转基因青蒿植株中表达的测定 总被引:3,自引:0,他引:3
目的 为了增强青蒿的特异药理活性,探讨人β-趋化因子RANTES基因在青蒿细胞中表达的可能性。方法 将克隆的RANTES基因经Ti质粒衍生的双元表达载体pRPKII导入根癌农杆菌LBA4404菌株,通过叶盘共培养法已获得一批表现卡那霉素抗性的转基因青蒿植株。结果 PCR和Southern印迹杂交分析表明,RANTES基因已导入并整合到青蒿基因组中;RT-PCR、Northern印迹、Western印迹杂交结果证实,RANTES基因已在转基因青蒿植株中成功表达。结论 首次报道了RANTES基因在转基因青蒿植株中的表达,为基因工程改造青蒿打下了良好的基础。 相似文献
134.
Three eudesmanolides, barrelierin (1), artemalin (2) and barrelin (3), isolated from aerial parts of Artemisia barrelieri, and desoxyvulgarin (4) obtained from barrelin by reduction with zinc and acetic acid, reduced carrageenan-induced rat paw oedema. The results show that the α-methylene γ-lactone group is not essential for the antiinflammatory effect, but variations in the molecular structure of these sesquiterpene lactones may enhance or diminish the antiinflammatory effect. 相似文献
135.
Byong Kweon Ryu Byoung Ok Ahn Tae Young Oh Soon Hoe Kim Won Bae Kim Eun Bang Lee 《Archives of pharmacal research》1998,21(5):508-513
The hepatoprotective effect of DA-9601, a quality-controlled extract ofArtemisia asiatica, on liver damage induced by acetaminophen (APAP) and carbon tetrachloride (CCI4) was investigated by means of serum-biochemical, hepatic-biochemical, and histopathological examinations. Doses of DA-9601
(10, 30, or 100mg/kg) were administered intragastrically to each rat on three consecutive days i.e. 48 h, 24 h and 2 h before
a single administration of APAP (640 mg/kg, i.p.) or CCl4 (2 ml/kg, p.o.). Four h and 24 h after hepatotoxin treatment, the animals were sacrificed for evaluation of liver damage.
Pretreatment of DA-9601 reduced the elevation of serum ALT, AST, LDH and histopathological changes such as centrilobular necrosis,
vacuolar degeneration and inflammatory cell infiltration dose-dependently. DA-9601 also prevented APAP- and CCl4-induced hepatic glutathione (GSH) depletion and CCl4-induced increase of hepatic malondialdehyde (MDA), a parameter of lipid peroxidation, in a dose-dependent manner. These findings
suggest that pretreatment with DA-9601 may reduce chemically induced liver injury by complex mechanisms which involve prevention
of lipid peroxidation and preservation of hepatic GSH. 相似文献
136.
Mamoon Ur Rashid Muhammad Alamzeb Saqib Ali Zahoor Ullah Zafar Ali Shah Ishrat Naz Muhammad Rafiullah Khan 《Phytotherapy research : PTR》2019,33(10):2661-2684
Several reviews have been published on Artemisia's derived natural products, but it is the first attempt to review the chemistry and pharmacology of more than 80 alkaloids and allied nitrogen compounds obtained from various Artemisia species (covering the literature up to June 2018). The pharmacological potential and unique skeleton types of certain Artemisia's alkaloids provoke the importance of analyzing Artemisia species for bioactive alkaloids and allied nitrogen compounds. Among the various types of bioactive Artemisia's alkaloids, the main classes were the derivatives of rupestine (pyridine–sesquiterpene), lycoctonine (diterpene), pyrrolizidine, purines, polyamine, peptides, indole, piperidine, pyrrolidine, alkamides, and flavoalkaloids. The rupestine derivatives are Artemisia's characteristic alkaloids, whereas the rest are common alkaloids found in the family Asteraceae and chemotaxonomically links the genus Artemisia with the tribes Anthemideae. The most important biological activities of Artemisia's alkaloids are including hepatoprotective, local anesthetic, β‐galactosidase, and antiparasitic activities; treatment of angina pectoris, opening blocked arteries, as a sleep‐inducing agents and inhibition of HIV viral protease, CYP450, melanin biosynthesis, human carbonic anhydrase, [3H]‐AEA metabolism, kinases, and DNA polymerase β1. Some of the important nitrogen metabolites of Artemisia include pellitorine, zeatin, tryptophan, rupestine, and aconitine analogs, which need to be optimized and commercialized further. 相似文献
137.
《Vaccine》2017,35(7):1037-1045
Activating innate immunity by an adjuvant is required in vaccine development. The study aims to investigate adjuvant effects of aqueous extracts of Artemisia rupestris L. (AEAR) in vivo and in vitro. ICR mice were subcutaneously administered with antigen and AEAR at various doses to evaluate their immune responses of antibodies, dendritic cells (DCs), regulatory T cells (Treg), splenic lymphocyte, and cytokine. The evaluation results showed that AEAR could largely increase titers of antigen-specific antibodies (IgG, IgG1, and IgG2a) and T cell proliferation. AEAR also increased expression of IFN-γ in CD8+T cells as well as IL-4 and INF-γ expression in CD4+T cells. Expression levels of MHC-II, CD40, CD80, and CD86 on DCs were significantly elevated, whereas the Treg frequency was significantly decreased. AEAR (200 μg) showed remarkable adjuvant activity. Furthermore, AEAR enhanced MHC-II, CD40, CD80, and CD86 expression as well as the yields of TNF-α and IL-12 on DCs through toll-like receptor4 (TLR4) in vitro. Those results indicated that AEAR could serve as an efficacious immune stimulator for vaccines because it significantly enhanced specific immune responses by promoting DCs maturation and reduced Treg through TLR4 signaling pathway. 相似文献
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140.
Effects and mechanisms of Artemisia argyi essential oil on monocrotaline-induced pulmonary hypertension in rats
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In the present study, we aimed to investigate the effects of Artemisia argyi essential oil (AO) on pulmonary hypertension (PH)inducedbymonocrotaline(MCT)andtoexploretheunderlying mechanism.A total of 80 Sprague-Dawley ratswererandomlydividedintofourgroups as follows:controlgroup, modelgroup,bosentan(0.1g/kg)groupandAO(0.1g/kg)group.After30dofexperiment, hemodynamicparameters,lungandrightventricle hypertrophy indexweredetermined. HEand Immunohistochemistrystainingoflungs were performedto detectthe injuriesandprotein expressions. Theresultsshowedthatlevels of mPAP,mRVP,maxRVP,wW,LI,RV and RVHI as well asthe expressionsofNF-κBp65andα-SMA were increased,smallpulmonaryarterythickened, the cavity of the arteriole narrowed, and there was marked infiltration of inflammatory cells in lungs of rats receiving MCT compared with the normal group. After the administration of AO, the levels of mPAP and mRVP were significantly decreased,and the wW, LI, LV+S and pulmonary arterial remodeling were markedly improved. The expression levels of NF-κB p65 and α-SMAwerereduced by AOcomparedwiththe modelgroup.Ourresultssuggestedthat AOreduced the progressionof PH induced byMCTthroughinhibiting the expressions of NF-κB p65 and α-SMA. 相似文献